Результаты поиска

Inhibition of migration but stimulation of proliferation of human retinal pigment epithelial cells cultured with uniform vesicles of silicone oil.

BACKGROUND: It has been hypothesized that emulsification of silicone oil might stimulate preretinal membrane formation and proliferative vitreoretinopathy. This study aimed to test the effects of vesicles of silicone oil (VSO), which were prepared by a novel membrane emulsification technique, on the migration and proliferation of human retinal pigment epithelial (hRPE) cells in vitro. METHODS: VSO were produced by extrusion of the oil through a Supor membrane (Pall Life Sciences, Port Washington, NY, USA) with 0.45 microm pore size under a pressure of 0.1 MPa. hRPE cells were incubated with s

A method for isolation and cultivation of adult Schwann cells for nerve conduit.

BACKGROUND: It has been found that one of the methods to repair peripheral nervous system or even central nervous system injury is to use Schwann cells as nerve regeneration promoters. Therefore, it seems necessary to look for a way to obtain activated Schwann cells, with a sufficient amount of numbers and purity, in a short time for clinical applications. However, the previous methods using mitogens are not much clinically acceptable, and other methods that do not require mitogens, fail to isolate adult Schwann cells effectively or require a long period of time. METHODS: In this study, Schwa

Quantification of cerebellar hemispheric purkinje cell linear density: 32 ET cases versus 16 controls.

Although essential tremor (ET) is among the most prevalent neurological diseases, its precise pathogenesis is not understood. Purkinje cell loss has been observed in some studies and is the focus of interest and debate. Expressing these data as Purkinje cells/layer length allows one to adjust for the inherent curved nature of the cerebellar folia. Capitalizing on the Essential Tremor Centralized Brain Repository, we quantified Purkinje cell linear density in cases versus controls. Free-floating 100-μm parasagittal cerebellar hemispheric sections were subjected to rabbit polyclonal anti-Calbind

Decreased proliferative, migrative and neuro-differentiative potential of postnatal rat enteric neural crest-derived cells during culture in vitro.

A growing body of evidence supports the potential use of enteric neural crest-derived cells (ENCCs) as a cell replacement therapy for Hirsch … expansion, proliferation, migration, apoptosis, and differentiation potentials were monitored. The Cell Counting Kit-8 was used for assessment of ENCC vitality, Transwell inserts for cell migration, immunocytochemistry for cell counts and identification, and flow cytometry for apoptosis. Over six continuous generations, ENCC proliferation potency was reduce …

Putative rabbit conjunctival epithelial stem/progenitor cells preferentially reside in palpebral conjunctiva.

PURPOSE: Conjunctival epithelia play an important role in forming a physical protective barrier of the ocular surface. To protect the integrity of the ocular surface, the conjunctival epithelial cells (CECs) must be self-renewing from local stem cells. In this study, the distribution of conjunctival epithelial stem/progenitor cells was determined. METHODS: In a long-term bromodeoxyuridine (BrdU) label-retaining study, adult New Zealand white rabbits were injected intraperitoneally with BrdU 50  mg/kg/d for 7 days and follow-up for 6 weeks. In the short-term BrdU label-retaining study, rabbits

Nutritional status impact on engraftment time in hematopoietic stem cell transplants (HSCT) in Semarang, Indonesia: A cross-sectional study.

BackgroundNutritional support is a critical consideration in the care of individuals undergoing hematopoietic stem cell transplantation (HSCT), given the metabolic alterations induced by intensive treatments. This study aimed to explore the correlation between nutritional status and the time to engraftment in HSCT patients at Dr. Kariadi General Hospital, Semarang, Indonesia.MethodsThis study included adult patients with malignant diseases who underwent autologous or allogeneic HSCT at Dr. Kariadi General Hospital between 2013 and 2023. Nutritional status was evaluated using body mass index (B

Predominantly cystic glioblastoma mimicking benign cystic lesions: A radiological diagnostic challenge.

Glioblastoma multiforme (GBM) is the most common and most aggressive primary malignant brain tumor in adults. Although it typically presents … ing complete blood count, erythrocyte sedimentation rate, C-reactive protein, and total white blood cell count, were within normal limits. Magnetic resonance imaging demonstrated a large predominantly cystic intra-axial lesion in the left frontal lobe with thick irregular peripheral enhancement, extensive surrounding vasogenic edema, and marked mass effect. …

Epigallocatechin-3-Gallate, the green tea polyphenol suppresses expression of CD31 and VEGFR2 and enhances the chemotherapeutic potential of irinotecan in AOM/DSS model of colorectal cancer in mice.

BACKGROUND: Conventional chemotherapeutic treatment of colorectal cancer causes adverse effects such as severe diarrhoea, mucositis and hepa … alleviation of colorectal cancer symptoms, improvement in histopathological alterations and goblet cell count with combination therapy. Suppression of key oncogenic and angiogenic markers and reduction in liver injury with combination treatment. CONCLUSION: Combined treatment of epigallocatechin-3-gallate with irinotecan enhanced the anti-tumour potential …

Lenalidomide-associated pure red cell aplasia during the treatment of POEMS syndrome: A case report and brief review of the literature.

Lenalidomide is widely used in the treatment of hematological malignancies, with bone marrow suppression being one of the most common hematological adverse effects associated with its use. However, lenalidomide-associated pure red cell aplasia (PRCA) remains exceptionally rare. The present study describes a case of PRCA secondary to lenalidomide therapy in a patient with POEMS syndrome. A 61-year-old woman diagnosed with POEMS syndrome received lenalidomide (25 mg/day) in combination with dexamethasone. Following 3 months of treatment, she developed severe anemia accompanied by a markedly redu

Совпадение в полном тексте источника
… Initial laboratory tests revealed the following: A white blood cell count (WBC) of 5.2x10 9 /l, a hemoglobin level of 86 g/l, a platelet count of 134x10 9 /l, and an absolute reticulocyte count (RET#) of 109.6x10 9 /l. …

Evaluation of Inflammatory Markers and Clinical Outcomes in Patients with Acute Pancreatitis: A Prospective Cohort Study.

BACKGROUND: Acute pancreatitis (AP) is a sudden inflammatory process of the pancreas that can lead to varying degrees of severity, ranging from mild discomfort to life-threatening conditions. Identifying key inflammatory markers at an early stage is crucial to predicting clinical outcomes and improving management. OBJECTIVE: This study aims to evaluate the association between inflammatory markers-C-reactive protein (CRP), serum amylase, and white blood cell (WBC) count-and clinical outcomes in patients with acute pancreatitis. METHODS: A prospective cohort study involving 120 patients diagno

Electroconvulsive therapy improves hematological inflammatory markers in bipolar disorder.

BACKGROUND: Immune dysfunction and inflammation participate in the pathophysiology of bipolar disorder (BD). Abnormal levels of inflammatory markers, namely, red cell distribution width (RDW) and platelet distribution width (PDW), were detected in BD. Electroconvulsive therapy (ECT) for various mental disorders including BD was linked to changes in these inflammatory mediators. Hence, we aimed to assess the impact of ECT on PDW and RDW in patients with BD. METHODS: Seventy-two patients aged ≥ 16 were enrolled in the current prospective cohort study over 6 months, diagnosed as BD based on DSM-

A retrospective evaluation of the effect of oclacitinib (Apoquel) administration on development of surgical site infection following clean orthopedic stifle surgery.

The aim of this study was to determine the effect of oclacitinib (Apoquel) on development of surgical site infections in canines following clean orthopedic stifle surgery. Medical records of dogs undergoing unilateral, clean orthopedic stifle procedures were retrospectively examined for development of post-operative surgical site infections. Data collected for statistical analysis included age, sex, body weight, current medications, anesthesia and surgery times, white blood cell count, and neutrophil count. Surgical site infections were identified in 8.7% (34/390) of stifle procedures- 8.0% (2…

Maternal blood parameters and risk of neonatal pathological jaundice: a retrospective study.

This study aims to investigate the association between maternal blood parameters and the risk of neonatal pathological jaundice. A retrospective case-control study of 1309 newborns and their mothers from 2019 to 2020 in a single-center tertiary hospital. All mothers received a complete routine blood test prior to delivery, and outcome was neonatal pathological jaundice. We performed stepwise logistic regression modeling to identify maternal blood factors associated with neonatal pathological jaundice. 258 neonates (19.71%) were diagnosed with pathological jaundice. Logistic regression results

Rare cause of voice muffling: pyogenic vallecular cyst.

A vallecular cyst is a benign laryngeal cyst that rarely causes symptoms in adults. We report a rare case of an infected vallecular cyst in a man who is in his 60s with diabetes mellitus. The patient presented to us with acute onset of voice muffling and respiratory distress. He showed signs of sepsis and upper airway obstruction. He had spikes of high-grade fever with an elevated total white cell count (predominantly neutrophil count) and C reactive protein level. Plain cervical X-ray revealed a large, smooth, lobulated mass in the vallecular area. Direct laryngoscopic findings supported the …

Severe spontaneous acute arterial subdural hematoma as an initial symptom of chronic myeloid leukemia.

Acute subdural hematoma (SDH) is a rare occurrence in chronic myeloid leukemia (CML) patients with only two cases reported in literature. However, sudden severe acute SDH caused by CML has not been reported on. Our patient was admitted for 'sudden unconsciousness for more than 1 hour'. Computed tomography (CT) angiography revealed a large amount of acute SDH on the left side. Physical exam showed the patient's left pupil was dilated and signs of cerebral herniation were present. The preoperative coagulation profile was normal. Emergency craniotomy for hematoma clearance and decompression was p

Hypercalcemia and Disseminated Osteolytic Lesions With Normal Blood Counts and Absence of Circulating Blasts: A Rare Presentation of Childhood B-Lymphoblastic Leukemia.

Hypercalcemia and disseminated osteolytic bone lesions are a rare presentation of pediatric acute lymphoblastic leukemia (ALL). The authors report a 3-year-old boy who presented with hypercalcemia and diffuse osteolytic lesions involving axial and appendicular bones. He had normal complete blood count and the absence of blasts in peripheral smear; however, bone marrow aspirate and trephine were consistent with B-cell ALL. A review of the literature highlights the variable clinical outcome of this rare presentation depending on the presence of hypercalcemia and osteolytic lesions with or withou

Paraneoplastic leukemoid reaction in a patient with sarcomatoid hepatocellular carcinoma: A case report.

BACKGROUND: Sarcomatoid hepatocellular carcinoma (SHC) combined with paraneoplastic leukemoid reaction (PLR), which is associated with a poor prognosis, is rarely seen in the clinic. Here, we report the case of a patient in the above situation. CASE SUMMARY: A 75-year-old female patient with a past medical history of hypertension and cerebral infarction paid a hospital visit as a result of right upper quadrant abdominal pain and anorexia for two months. Laboratory examination revealed a white blood cell (WBC) count of 43790/μL, which was then increased up to 77050/μL. In addition, the results

[Relevance of complete blood count parameters in the assessment of acute coronary syndromes: a combined hematological and cardiological perspective].

The aim of this review is to explore the available evidence concerning the relationship between the different parameters of the complete blood count, its pathophysiological changes and cardiovascular disease, specifically focusing on the acute ischemic setting. Erythrocytes, leukocytes and platelets undergo significant and more or less durable changes over time in response to conditions of systemic inflammatory, infectious and neoplastic disease. This is the reason why blood cell count parameters can (and should) be implemented in the global assessment of the patient with acute coronary syndro…

The Relationship of Mean Platelet Volume/Platelet Distribution Width and Duodenal Ulcer Perforation.

BACKGROUND: Duodenal ulcer perforation (DUP) is a severe acute abdominal disease. Mean platelet volume (MPV) and platelet distribution width (PDW) are two platelet parameters, participating in many inflammatory processes. This study aims to investigate the relation of MPV/PDW and DUP. METHODS: A total of 165 patients were studied retrospectively, including 21 females and 144 males. The study included two groups: 87 normal patients (control group) and 78 duodenal ulcer perforation patients (DUP group). Routine blood parameters were collected for analysis including white blood cell count (WBC),…

Comparison of the Single-Syringe Push-Pull Technique With the Discard Technique for Obtaining Blood Samples From Pediatric Central Venous Access Devices.

The discard technique is the most widely used method of obtaining blood samples from patients with central venous access devices (CVADs), bu … nificant differences for sodium, potassium, chloride, bicarbonate, creatinine, calcium, white blood cell count, hemoglobin, hematocrit, and platelet count, but these differences were not clinically significant and within the standard error of measurement for the instrument. Given the similarity in laboratory results, the push-pull method should be considered …