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A comparison of 13 guinea pig and human anti-tissue transglutaminase antibody ELISA kits.

AIMS: Tissue transglutaminase (tTG) is a major autoantigen recognised by IgA anti-endomysial antibodies (IgA EMA). Enzyme linked immunosorbent assays (ELISA) for IgA anti-tissue transglutaminase antibodies (IgA tTG) have therefore been developed as an alternative serological screening test to IgA EMA for coeliac disease (CD). The use of human tTG (h-tTG), as opposed to guinea pig liver tTG (gpl-tTG), in these assays has been reported to produce superior results. This study compared 13 commercial IgA tTG ELISA kits to ascertain their performance characteristics in the diagnosis of CD in patient

Cardiomyopathy associated with celiac disease in childhood.

Celiac disease is predominantly a disease of the small intestine characterized by chronic malabsorption in genetically susceptible individuals who ingest grains containing gluten, such as wheat, barley, and rye. Although previously believed to be uncommon, celiac disease may be present in up to 1% of the adult and children population. Celiac disease is associated frequently with iron-deficiency anemia, dermatitis herpetiformis, selective IgA deficiency, thyroid disorders, diabetes mellitus, and various connective tissue disorders but is rarely associated with cardiomyopathy.

Microecology of the gastrointestinal tract and the immunological status under food allergy.

In children suffering from food allergy the microecology of the gut and the immune status were examined. As a rule, sensitization due to the penetration of the epithelial barrier of the intestinum by macromolecules of the food is not accompanied by deficiency of secretory IgA. In all cases dysbacteriosis of different degree was observed with a characteristic picture of deficiency of Bifidobacteria and Lactobacilli combined with rising numbers of Enterobacteriaceae. Correlations to an altered immune response are obvious.

Respiratory tract epithelial cells express retinaldehyde dehydrogenase ALDH1A and enhance IgA production by stimulated B cells in the presence of vitamin A.

Morbidity and mortality due to viral infections are major health concerns, particularly when individuals are vitamin A deficient. Vitamin A deficiency significantly impairs mucosal IgA, a first line of defense against virus at its point of entry. Previous reports have suggested that CD11c(Hi) dendritic cells (DCs) of the gastrointestinal tract produce retinaldehyde dehydrogenase (ALDH1A), which metabolizes vitamin A precursors to retinoic acid to support normal mucosal immunity. Given that the upper respiratory tract (URT) and gastrointestinal tract share numerous characteristics, we asked if

Mode of presentation and performance of serology assays for diagnosing celiac disease: A single-center study in the United Arab Emirates.

OBJECTIVE: To characterize patients with celiac disease (CD), examines the clinical spectrum of CD, and evaluate the performance of serologic tests used for CD screening, in the United Arab Emirates (UAE). METHODS: Medical charts of patients received at the Digestive Diseases Institute of Cleveland Clinic Abu Dhabi from January 2015 to December 2020 were reviewed. Patients who were screened for four serologic biomarkers (anti-tissue transglutaminase IgA [Anti-tTG-IgA], anti-tissue transglutaminase IgG [Anti-TtG-IgG], anti-deamidated gliadin peptide IgG [Anti-DGP-IgG], and anti-deamidated glia

Salivary Immunoglobulins in Individuals with Common Variable Immunodeficiency.

Oral manifestations of common variable immunodeficiency (CVID) are rare, have rarely been studied and have given controversial results. There are few data about IgA, IgG, and IgM antibody salivary levels in the literature, and there are few papers about the clinical impact of antibody deficiencies and CVID on the oral health of such patients. The aim of this study was to measure serum and salivary IgA, IgG, and IgM levels in CVID participants and controls, and to associate immunoglobulin levels with caries and periodontal disease. This was a case-control study involving 51 CVID individuals and

Tissue transglutaminase antibodies in patients with end-stage heart failure.

OBJECTIVE: For celiac disease (CD), screening a trend has recently emerged to measure tissue transglutaminase antibodies (tTGA) by immunoass … atients with end-stage heart failure and 60 blood donors. No subject had clinical evidence of CD or IgA deficiency, and all were EmA negative. Serum IgA and IgG tTGA were measured by means of commercial kits using as substrate, either guinea pig or recombinant human tTG. Blocking studies and Western blots were also performed using recombinant human tTG. RESULTS …

Pediatric IgA-Dominant Infection-Related Glomerulonephritis.

The concept of infection-related glomerulonephritis (IRGN) has been introduced as adults diagnosed with glomerulonephritis often have coexisting active infections. Furthermore, IgA-dominant IRGN is associated with staphylococcal infections in adults with comorbidities, which often progress to end-stage renal disease. Little is known about IgA-dominant IRGN in children, and no consensus for a management strategy of this condition has been reached. We describe the case of a 9-year-old boy with IgA-dominant IRGN that was diagnosed using specific staining for nephritis-associated plasmin receptor

IgG3 deficiency: common in obstructive lung disease. Hereditary in families with immunodeficiency and autoimmune disease.

Among 313 patients with serum IgG deficiency, selective IgG3 deficiency was found in 59.5%, combined IgG3 deficiency together with IgG1 defi … . IgG3 deficiency was also found in relatives of patients with common variable immunodeficiency and IgA deficiency. In one family both parents and all four children showed IgG3 deficiency, some of them also C2 deficiency. In another family 2 siblings with diabetes mellitus showed IgG3 deficiency. In still another family the mother and her daughter both with asth …

Immune dysregulation, apoptosis impairment, and enhanced seroreactivity to Anisakis simplex in Crohn's disease: interplay of IL-7/IL-7R signalling and CD132 deficiency.

BACKGROUND: In previous studies, we identified a deficiency of γδ T cells and an increased prevalence of anti-Anisakis simplex antibodies in patients with Crohn's disease (CD). Additionally, decreased gene expression of the interleukin 2 (IL-2) receptor γ subunit (CD132) was observed in tissues from CD patients. OBJECTIVE: To analyse the gene expression of IL-7 and its receptors in tissues from CD patients and to explore its relationship with anti-A. simplex antibodies. METHODS: 52 patients diagnosed with CD were compared with a control group of 52 healthy individuals. Peripheral blood sampl

AGA Clinical Practice Update on Diagnosis and Monitoring of Celiac Disease-Changing Utility of Serology and Histologic Measures: Expert Review.

PURPOSE: The purpose of this clinical practice update is to define key modalities in the diagnosis and monitoring of celiac disease (CD) in … enal biopsies may then be performed for purposes of differential diagnosis. Best Practice Advice 3: IgA deficiency is an infrequent but important explanation for why patients with CD may be negative on IgA isotype testing despite strong suspicion. Measuring total IgA levels, IgG deamidated gliadin antibody tests, and TG2-IgG testing in that circumstance is recom …

Nutritional status in pediatric celiac disease: evaluation at diagnosis and during follow-up.

INTRODUCTION: Celiac disease (CD) is a lifelong immune-mediated condition precipitated by gluten ingestion in genetically predisposed individuals, resulting in intestinal mucosal damage, impaired growth, micronutrient deficiencies, among other gastrointestinal and extraintestinal symptoms. A gluten-free diet (GFD) contributes to the healing of the mucosa and improves nutrient absorption, although recovery patterns may vary. AIM: The present study aimed to determine changes in anthropometric parameters and micronutrient levels at diagnosis and at six and twelve months following the start of a

Primary cutaneous marginal zone lymphoma with sequential development of nodal marginal zone lymphoma in a patient with selective immunoglobulin A deficiency.

Multiple lymphoma subtypes occurring within one patient is rare in the context of B-cell lymphoma, and only few such cases have been reported in association with primary cutaneous marginal zone lymphoma (PCMZL). We herein describe the case of a 43-year-old patient who was diagnosed with PCMZL and subsequently developed a clonally unrelated nodal marginal zone lymphoma (MZL). At the time of diagnosis of PCMZL, multiple skin lesions were present. The atypical lymphoid infiltrate showed monotypic expression of immunoglobulin light chain lambda and heavy chain (IgM) on immunohistochemistry and an

[Rare hyperimmunoglobulinemia E syndromes].

Total immunoglobulin E levels are very frequently measured, particularly for allergy. Their high levels are ordinarily found in atopy and parasitosis. This article subject is to determine rare diseases where high levels of total immunoglobulin E are found except in classic diseases. These rare hyper-immunoglobulin E syndromes are more often associated with immunologic deficiency more or less intense that lead to viral, fungal and bacterial infections. Buckley syndrome: deep infections due to Staphylococcus aureus, chronic dermatitis. Netherton disease: cutaneous and hair disease, allergic mani

[FREQUENCY OF IMMUNOGLOBULIN E DEFICIENCY AMONG PATIENTS WITH IMMUNODEPENDENT DISORDERS].

Isolated IgE deficiency is one of the most common primary immunodeficiency, which is still underestimated cause of health disorders of modern man. Recent genetic studies report that the cause of the IgE deficiency is immune dysregulation caused by polymorphisms of the gene, which is responsible for the synthesis of activation-induced cytidinedeaminase (AICDA). The Institute of Immunology and Allergology at Bogomolets NMU during the years 2012-2014 were examined 5298 patients with a range of different diseases, which could be suspected violations of immunity. All patients were conducted compreh

[The hyper-IgM syndrome: 13 observations].

INTRODUCTION: The hyper-IgM syndrome (HIGM) is a rare hereditary immune deficiency, characterised by a low or nil level of IgG and IgA and a normal or increased level of IgM, predominately affecting boys. Its clinical manifestations are dominated by recurrent infection, notably of the digestive tube, the ears nose and throat and the lungs. METHODS: We conducted a retrospective study of the medical files of 13 patients with 10 boys and 3 girls from 12 different families. In all the patients, the diagnosis was established on measuring the serum immunoglobulines IgG, IgA and IgM using Mancini's

Serum sBCMA в первичном и вторичном дефиците антител.

B-клеточное антигенное созревание (BCMA) — рецептор поверхности клетки, регулирующий активацию, распространение и выживание клеток B. BCMA может быть отделена от поверхности клеток, производя растворимую BCMA (SBCMA), которая была изучена как биомаркер болезней в системном волчании эритематосуса, множестве склероза и миеломе. Снижение концентраций SBCMA связано с остротой различных основных недостатков антител. Мы изучили взаимосвязь между концентрациями SBCMA, юморальными иммунными реакциями на вакцинацию SARS-COV-2 и осложнениями при заболеваниях у 107 человек с первичным (PAD) и вторичным д

Совпадение в аннотации оригинала
B-cell maturation antigen (BCMA) is a B cell surface receptor that regulates B cell activation, proliferation and survival. BCMA can be clea … ntrations were significantly reduced in PAD compared to healthy controls and asymptomatic selective IgA deficiency. Individuals with X- linked agammaglobulinemia and common variable immunodeficiency (CVID) demonstrated the lowest serum concentrations of sBCMA. sBCMA concentrations in SAD were highly variable. Amongst individuals with CVID, peripheral blood CD19 …

[Some current aspects of diagnosis of calculous pyelonephritis].

Immunomicrobiological examination was made of 656 urine and 71 blood samples from 71 patients with chronic pyelonephritis and coral nephrolithiasis. Bacteriuria in blood agar was quantified in colony-forming cells (CFC) in 1 ml of the urine. Identification of the bacterial strains was made by conventional methods. Blood samples were examined for phagocytic activity (PA) of neutrophils and phagocytic index (PI) in incomplete (30 min) and complete (2 hours) variants (S.aureus-209P), levels of IgA, IgM, IgG (in IU/ml), complement (CH50), T- and B-lymphocytes and 0-cells. Opportunistic bacteria (O

1040 prophylactic infusions with an unmodified intravenous immunoglobulin product causing few side-effects in patients with antibody deficiency syndromes.

Thirty-two patients with common variable immunodeficiency (CVID) and two patients with IgA and IgG subclass deficiency received a total of 1,040 intravenous (i.v.) infusions during 60 patient years with 7,575 g of a new immunoglobulin (Ig) preparation. The content of prekallikrein activators and the anti-complementary activity in the tested Ig preparation was low and, in comparison to seven other commercial i.v. Igs, so was the proportion of IgG polymers and fragments. The IgA content was always less than or equal to 0.02 g/l, often less than 0.004 g/l, and it was possible to continuously give