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Plasma Proteomic Signature as a Predictor of Age Advancement in People Living With HIV.

Due to the increased burden of non-AIDS-related comorbidities in people living with HIV (PLHIV), identifying biomarkers and mechanisms underlying premature aging and the risk of developing age-related comorbidities is a priority. Evidence suggests that the plasma proteome is an accurate source for measuring biological age and predicting age-related clinical outcomes. To investigate whether PLHIV on antiretroviral therapy (ART) exhibit a premature aging phenotype, we profiled the plasma proteome of two independent cohorts of virally suppressed PLHIV (200HIV and 2000HIV) and one cohort of people…

The efficacy of teclistamab in patients with multiple myeloma and secondary plasma cell leukemia.

INTRODUCTION: Hepatitis E virus (HEV) is a global cause of acute viral hepatitis, for which there is currently no FDA-approved medication. This case report describes a unique instance of a healthy US female presenting with acute HEV, who was treated with ribavirin to address her acute liver injury. CASE PRESENTATION: A 36-year-old US female, nonpregnant, without liver disease, developed nonspecific symptoms post-travel to Indonesia, leading to acute liver injury with concern for failure. Liver biopsy showed severe hepatocellular necrosis (>50%) with mixed infiltrate; HEV PCR was positive (93,

Identifying plasma exosome antigens as a potential diagnostic biomarker for tuberculosis disease.

BACKGROUND: Exosome is a small extracellular vesicle with a diameter of 30 to 150 nm that is secreted by cells. Mtb and other bacteria can also secrete extracellular vesicles, which carry characteristics and information about the pathogen. Here, we compare the concentration of exosomes and the Mtb antigen in exosomes of tuberculosis patients aiming to evaluate whether exosomes can be used as diagnostic markers of tuberculosis at different stages. METHODS: Plasma exosomes were extracted from healthy individuals, the patients with active TB and latent TB, Rehabilitation patients. The concentrat…

A Plasma Metabolic Signature to Diagnose Pulmonary Tuberculosis and Monitor Treatment Response.

BACKGROUND: High-resolution metabolomics has shown promise for identifying blood-based biomarkers of tuberculosis (TB). We sought to discover a metabolic signature to detect pulmonary TB disease and monitor treatment response. METHODS: Plasma from Ethiopian persons with pulmonary TB at diagnosis (n = 82) was compared to household contacts with TB symptoms (n = 104) and 2, 6, and 12 months after treatment initiation. Participants were divided into training and test sets for model building, with additional validation using independent cohorts from the countries of Georgia (n = 89) and South Afr…

The gut dysbiosis and plasma lipid metabolisms signatures in children with active tuberculosis.

BACKGROUND: The human gut microbiota is an important modulator of host immune responses and has a crucial role in the development of tuberculosis (TB). Evidences suggest that metabolites may function as a bridge between gut microbiome and TB progression in children. However, the underlying interactive mechanisms are not well explored. The results may provide useful insight into the role played by the gut microbiome in pulmonary TB in children. METHODS: To explore the gut bacterial features and its interaction with plasma lipid metabolisms in children with TB. We enrolled children aged younger …

Metabolomics and lipidomics of plasma biomarkers for tuberculosis diagnostics using UHPLC-HRMS.

INTRODUCTION: Determining metabolic profiles during host-pathogen interactions is crucial for developing novel diagnostic tests and explorin … et, while 30 TB patients and 30 HCs were enrolled as the independent validation set. Metabolites in plasma samples were analyzed by high-resolution mass spectrometry. Differential metabolites were screened using principal component analysis and machine learning algorithms including LASSO, Random Forest, and XGBoost. The diagnostic accuracy of the core di …

In Vivo Evaluation of Laser-Textured Air Plasma in Osseointegration of Dental Implants.

The different macro and micro geometries of dental implants are parameters that directly affect osseointegration, making them an important area for research. The objective of this preclinical study was to compare, through histological and histomorphometric analyses, the biological response of two different dental implant surfaces in osseointegration. Surface morphology and chemistry were characterized by SEM/EDX, optical-emission spectroscopy, protein adsorption (BSA), and adipose-derived stem-cell morphology. For the in vivo arm, ten commercially pure titanium implants (n = 5 LS160 + 5 SBAE)

Entamoeba histolytica: Plasma membrane components and virulence factors in the invasive process.

Entamoeba histolytica, the etiological agent of amoebiasis, is a parasitic protozoan responsible for severe gastrointestinal complications, including colitis and hepatic abscesses. The parasite primarily colonizes the human large intestine, where the active stages confront a hostile environment. Specialized adaptations in the plasma membrane of E. histolytica are critical for survive in this niche and enable the parasite to persist harmlessly in asymptomatic infection or initiate pathogenic interactions that lead to tissue invasion. Early events in pathogenesis are the breaching of the intesti…

Безопасность и эффективность терапевтического плазменного обмена педиатрическими нейро-иммунологическими заболеваниями.

ЦЕЛЬ: Терапевтический плазменный обмен (TPE) является предпочтительным методом лечения различных нейро-иммунологических расстройств у педиатров. В этом исследовании дается оценка безопасности и эффективности ПТТ в этой демографической области. МАТЕРИАЛЫ И МЕТОДЫ: Было проведено одноцентральное, ретроспективное когортное исследование с участием педиатрических пациентов, которые пережили нейроиммунологические явления и прошли процедуру ТОП в третичном специализированном центре. В исследовании приняли участие 81 пациент (Guillain-Barre: 65, полиневропаты: 5, миастения гравис: 8, множественный скл

Совпадение в аннотации оригинала
PURPOSE: Therapeutic plasma exchange (TPE) is the treatment of choice in various neuro-immunological disorders in pediatric populations. This study assesses the safety and efficacy of TPE in this demographic. MATERIALS AND METHODS: A single-center, retrospective cohort study involving pediatric patients who experienced neuro-immunological events and underwe…

MYO1F positions cGAS on the plasma membrane to ensure full and functional signaling.

Cyclic guanosine monophosphate (GMP)-adenosine monophosphate (AMP) synthase (cGAS) detects viral or endogenous DNA, activating the innate immune response to infections and autoimmune diseases. Upon binding to double-stranded DNA, cGAS synthesizes 2'3' cGMP-AMP, which triggers type I interferon production. Besides its presence in the cytosol and nucleus, cGAS is found at the plasma membrane, although its significance remains unclear. Here, we report that cGAS associates with myosin 1F (MYO1F) at the plasma membrane of human and mouse macrophages. During viral infection, phosphorylation of MYO1F…

Cell-Penetrating Drug Carrier by Molecular Recognition of Sphingomyelin on Plasma Membranes.

Plasma membranes not only maintain the intracellular microenvironment through their phospholipid bilayer but also eliminate exogenous compounds outside the cell membranes. Most drugs especially with high polarity are prevented from entering into cells to exert their effects. Therefore, it is of great significance to design effective drug carriers with a penetrating ability toward plasma membranes. In this study, a dual-templated MIP (dt-MIPs) carrier with controllable microstructure and high drug loading capacity was prepared using highly expressed sphingomyelin on the plasma membrane and teno…

Effect of plasma exosome lncRNA on isoproterenol hydrochloride-induced cardiotoxicity in rats.

Isoprenaline hydrochloride (IH) is a β-adrenergic receptor agonist commonly used in the treatment of hypotension, shock, asthma, and other diseases. However, IH-induced cardiotoxicity limits its application. A large number of studies have shown that long noncoding RNA (lncRNA) regulates the occurrence and development of cardiovascular diseases. This study aimed to investigate whether abnormal lncRNA expression is involved in IH-mediated cardiotoxicity. First, the Sprague-Dawley (SD) rat myocardial injury model was established. Circulating exosomes were extracted from the plasma of rats and ide…

Discovering the Radiation Biomarkers in the Plasma of Total-Body Irradiated Leukemia Patients.

The increased risk of acute large-scale radiological exposure for the world's population underlines the need for optimal radiation biomarkers. Ionizing radiation triggers a complex response by the genome, proteome, and metabolome, all of which have been reported as suitable indicators of radiation-induced damage in vivo. This study analyzed peripheral blood samples from total-body irradiation (TBI) leukemia patients through mass spectrometry (MS) to identify and quantify differentially regulated proteins in plasma before and after irradiation. In brief, samples were taken from 16 leukemic pati…

Transcriptome analysis of Candida albicans planktonic cells in response to plasma medicine.

Introduction. Cold plasma is frequently utilized for the purpose of eliminating microbial contaminants. Under optimal conditions, it can function as plasma medicine for treating various diseases, including infections caused by Candida albicans, an opportunistic pathogen that can overgrow in individuals with weakened immune system.Gap Statement. To date, there has been less molecular study on cold plasma-treated C. albicans.Research Aim. The study aims to fill the gap in understanding the molecular response of C. albicans to cold plasma treatment.Methodology. This project involved testing a col…

Differential distribution of ivacaftor and its metabolites in plasma and human airway epithelia.

Ivacaftor is the first clinically approved monotherapy potentiator to treat CFTR channel dysfunction in people with cystic fibrosis. Ivacaftor (Iva) is a critical component for all current modulator therapies, including highly effective modulator therapies. Clinical studies show that CF patients on ivacaftor-containing therapies present various clinical responses, off-target effects, and adverse reactions, which could be related to metabolites of the compound. In this study, we reported the concentrations of Iva and two of its major metabolites (M1-Iva and M6-Iva) in capillary plasma and estim…

The Inflammasome Adaptor Protein ASC in Plasma as a Biomarker of Early Cognitive Changes.

Dementia is a group of symptoms including memory loss, language difficulties, and other types of cognitive and functional impairments that a … ght chain (NfL), glial fibrillary acidic protein (GFAP), and amyloid-β 42/40 (Aβ42/40) ratio in the plasma of older adults. Participants had blood drawn at baseline and underwent two annual clinical and cognitive evaluations. The groups tested either cognitively normal on both evaluations (NN), cognitively normal year 1 but cognitively impaired year 2 (N …

Changes in the plasma protein-binding rate of remifentanil during cardiopulmonary bypass.

AIMS: Cardiopulmonary bypass (CPB) reduces the plasma protein-binding rate of some anaesthetics and can enhance their pharmacological effects by increasing the unbound drug fraction. However, whether these changes occur with remifentanil remains to be explored. We investigated the changes in the protein-binding rate of remifentanil during CPB compared with propofol. METHODS: Thirteen patients (≥18 years old) who were scheduled to undergo cardiovascular surgery with CPB were included. Arterial blood samples were collected to measure the plasma concentrations of remifentanil and propofol before…

Decreased levels of phosphorylated synuclein in plasma are correlated with poststroke cognitive impairment.

JOURNAL/nrgr/04.03/01300535-202509000-00022/figure1/v/2024-11-05T132919Z/r/image-tiff Poststroke cognitive impairment is a major secondary effect of ischemic stroke in many patients; however, few options are available for the early diagnosis and treatment of this condition. The aims of this study were to (1) determine the specific relationship between hypoxic and α-synuclein during the occur of poststroke cognitive impairment and (2) assess whether the serum phosphorylated α-synuclein level can be used as a biomarker for poststroke cognitive impairment. We found that the phosphorylated α-synuc

Insufficient Plasma Melatonin and Its Association With Neuropsychiatric Impairments in Patients With T2DM.

Purpose: Type 2 diabetes mellitus (T2DM) is associated with multiple neuropsychiatric impairments, including cognitive dysfunction, and melatonin (MLT) plays a crucial role in maintaining normal neuropsychiatric functions. This study is aimed at investigating the change in plasma MLT levels and its association with neuropsychiatric impairments in T2DM patients. Methods: One hundred twenty-six T2DM patients were recruited, and their demographics and clinical data were collected. Apart from the plasma glycated hemoglobin (HbA1c) levels and other routine metabolic indicators, the plasma concentra…