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Circular RNA circPTPRF promotes the progression of GBM via sponging miR-1208 to up-regulate YY1.

Glioblastoma (GBM) is the most common primary malignant tumor in the brain, and its robust proliferation and invasion abilities reduce the survival time of patients. Circular RNAs (circRNAs) play an essential role in various tumors, such as regulating tumor cell proliferation, apoptosis, invasion, metastasis, and other progressive phenotypes through different mechanisms. Finding novel circRNAs may significantly contribute to the prognosis of GBM and provide the basis for the targeted therapy of GBM. In this study, we found circPTPRF is a novel circRNA that has never been studied, which was hig

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… Use IMAGE J software (National Institutes of Health, Bethesda, MD, USA) and ECL kits (#P0018S, Beyotime Biotechnology, Beijing, China) for quantification. Immunofluorescence Perform immunofluorescence as depicted previously [ 34 ]. … Xenograft experiments Inject transfected GSCs (5 × 10 4 cells per mice) orthotopically into the brains of 6-week-old female BALB/c nude mice (Beijing Vital River Laboratory Animal Technology, Beijing, China). …

ICIMH 2022 Abstracts

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Микробные профили пациентов с оптическим неуритом или миастенией гравис.

ЦЕЛЬ: Характеристика кишечного микробного состава пациентов с оптическим невритом (ON) или миастенией грависа (MG). МЕТОДЫ: Пробы стула были собраны у 45 пациентов с НО, 13 пациентов с MG и 20 здоровых методов контроля. Микробиальная геномная ДНК была извлечена, и V3-V4 области бактериальных генов 16S rRNA были расширены и секвенированы. Для сопоставления альфа-разнообразия, бета-разнообразия, таксономических задач и бактериального богатства групп был проведен биоинформатический анализ. Различия в изобилии микробных таксов были выявлены с использованием линейного анализа эффекта дискриминантов

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… Materials and methods Selection criteria for the participants We performed a prospective study that was approved by the Ethics Committee of Beijing Tongren Hospital, Capital Medical University (approval number TRECKY2018-081) and conducted in accordance with the principles of the Declaration of Helsinki … Results Study sample and stool sample collection Stool samples were collected from 173 patients (137 with ON and 36 with MG) at the Neurology Department of Beijing Tongren Hospital, Capital Medical University, between 1 February 2018 and 30 December 2020. …

Comparison of the postoperative pain change and spinal stenosis rate between percutaneous vertebroplasty combined with radiofrequency ablation and with 125I particle implantation in the treatment of metastatic spinal cord compression: A retrospective study.

BACKGROUND CONTEXT: Metastatic spinal cord compression (MSCC) seriously affects the survival rate. OBJECTIVE: The therapeutic effects of two treatment strategies for MSCC: percutaneous vertebroplasty (PVP) combined with radiofrequency ablation (RFA) and PVP combined with 125I particle implantation, were compared. STUDY DESIGN: Retrospective study. PATIENT SAMPLE: 40 patients with MSCC were divided into two groups: 19 cases in the RFA group and 21 cases in the 125I group. METHOD: All patients were accessed to determine the differences in pain, which was evaluated using the visual analog sca

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… The following materials and equipment were obtained: polymethyl methacrylate (PMMA) (Heraeus Medical GmbH, Germany), high-temperature disinfection barium sulfate (Qingdao Oriental Chemical Co., Ltd., China), three-dimensional treatment planning system (Beijing Feitianzhao Industry Co., Ltd., China), … 18G particle implant needle and device (Beijing Feitianzhao Industry Co., Ltd., China), 125 I particle (Shanghai Xinke Pharmaceutical Co., Ltd., China), digital subtraction angiography (DSA) system (GE, Innova IGS 630, USA), 3.0T MRI (GE, USA), and 64-slice spiral CT (Toshiba, Japan). …

Effects of human umbilical cord mesenchymal stem cells derived from exosomes on migration ability of endometrial glandular epithelial cells.

The present study aimed to investigate the effects of human umbilical cord mesenchymal stem cells (Huc‑MSCs)‑derived exosomes on the migratory abilities of endometrial glandular epithelial cells, and to evaluate the underlying mechanism from the perspective of epithelial‑mesenchymal transition (EMT). Huc‑MSCs were prepared from human umbilical cord, and eutopic endometrial glandular epithelial cells were isolated from patients with endometriosis. The exosomes derived from Huc‑MSCs (Huc‑MSCs‑exo) were prepared using an exosome extraction kit. The endometrial glandular epithelial cells were rand

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… H1045; Beijing Solarbio Science & Technology Co., Ltd.) containing antibiotics, and blood vessels and impurities in the tissue were shaved using a scalpel. … One day after seeding, the cells in the lower chamber were fixed with 4% paraformaldehyde for 30 min at room temperature and stained with 1% crystal violet (Beijing Solarbio Science & Technology Co., Ltd.) for 5 min at room temperature. …

[Effect of intestinal obstruction stent combined with neoadjuvant chemotherapy on the pathological characteristics of surgical specimens in patients with complete obstructive colorectal cancer].

Objective: To compare the effects of three treatment options: emergency surgery, stent-surgery, and stent-neoadjuvant chemotherapy-surgery, on the pathological characteris- tics of surgically-resected specimens from patients with completely obstructive colorectal cancer. Methods: This was a retrospective cohort study analyzing clinicopathological data of patients with complete obstructive colorectal cancer who were admitted to the General Surgery Department of Beijing Chaoyang Hospital, Capital Medical University, between May 2012 and August 2020. The inclusion criteria were diagnosed with com…

Efficacy and safety of COVID-19 vaccines.

BACKGROUND: Different forms of vaccines have been developed to prevent the SARS-CoV-2 virus and subsequent COVID-19 disease. Several are in … ma/Pfizer), mRNA-1273 (ModernaTx), ChAdOx1 (Oxford/AstraZeneca), Ad26.COV2.S, BBIBP-CorV (Sinopharm-Beijing), and BBV152 (Bharat Biotect) reduce the incidence of symptomatic COVID-19 compared to placebo (vaccine efficacy (VE): BNT162b2: 97.84%, 95% CI 44.25% to 99.92%; 2 RCTs, 44,077 participants; mRNA-1273: 93.20%, 95% CI 91.06% to 94.83%; 2 RCTs, 31,632 …

Association of the Endothelial Activation and Stress Index (EASIX) with short-term mortality in critically ill patients with congestive heart failure: A retrospective cohort study from the MIMIC database.

The role of endothelial injury in worsening congestive heart failure (CHF) remains unquantified. This study evaluates the Endothelial Activation and Stress Index (EASIX) for predicting short-term mortality in patients with critical CHF. This was a retrospective cohort study using the Medical Information Mart for Intensive Care-IV (2008-2022). Adults with CHF admitted to the intensive care unit were stratified by ln(EASIX) quartiles. The primary endpoint was 30-day all-cause mortality. Multivariable Cox regression, restricted cubic splines, and subgroup analyses were performed. Among 4556 patie

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… All analyses were performed using R (version 4.4.2; R Foundation for Statistical Computing, Vienna, Austria) and Free Statistics software (version 2.1; FreeClinical Medical Technology Co., Ltd., Beijing, China). Statistical significance was considered when the 2-sided P value was <.05. 3. … Acknowledgments We thank Dr Liu Jie (People’s Liberation Army of China General Hospital, Beijing, China) for helping in this revision. Author contributions Methodology: Yingxiu Huang. Investigation: Ming Hu. Project administration: Ming Hu. Supervision: Peng Zhen, Xinzhen Li. …

Targeting ADAR1 Restores Interferon Signaling and Enhances Immunotherapy Response in Multiple Myeloma.

Multiple myeloma (MM) remains an incurable hematological malignancy in which tumor-intrinsic immune evasion limits the efficacy of immunotherapy. Here, we identify the RNA editing enzyme ADAR1 as a key regulator of innate immune suppression in MM. Integrative analyses of bulk and single-cell transcriptomic datasets, together with clinical validation, demonstrated that ADAR1 is upregulated in malignant plasma cells and is associated with adverse clinical outcomes and reduced CD8+ T-cell infiltration. Mechanistically, ADAR1 knockdown increased the association of endogenous dsRNA with melanoma di

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… MM cells with CD8 + T cells in the absence of HS-5 cells (NC-). 4.15. siRNA Transfection Small interfering RNAs (siRNAs) targeting ADAR1, MDA5, and STAT1, as well as a negative control siRNA (si-NC), were transfected into U266 and H929 cells using CALNP™ RNAi transfection reagent (DN001, D-Nano, Beijing … Apoptosis was evaluated using Annexin V-FITC/PI staining (SC123, Beijing, China) followed by flow cytometry analysis with FlowJo software (version 10.8.1). 4.21. …

Выявление потенциальных нервных сигналов, вызванных наркотиками, путем проведения непропорционального анализа базы данных FAERS.

Нейроргия, вызванная наркотиками, является широко распространенной и значительной негативной реакцией. В рамках этого исследования была проанализирована база данных системы представления информации о неблагоприятных явлениях (FAERS) в Соединенных Штатах Америки (продовольствие и наркотики)2004-2024) выявления соответствующих наркотиков и потенциальных механизмов. Мы провели анализ связей между наркотиками и нейронгией с использованием базы данных FAERS. Применялись методы непропорционального анализа, включая коэффициент вероятности представления отчетности (ROR), коэффициент пропорциональной о

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… Acknowledgments We would like to thank our colleagues at the Fourth Hospital of Hebei Medical University, Beijing Health Vocational College, and Beijing Jishuitan Hospital, Capital Medical University, for their valuable support and constructive suggestions throughout this research. …

Factors influencing further vertebral height loss following percutaneous vertebroplasty in osteoporotic vertebral compression fractures: A 1-year follow-up study.

BackgroundOsteoporotic vertebral compression fractures (OVCFs) contribute to back pain and functional limitations in older individuals, with percutaneous vertebroplasty (PVP) emerging as a minimally invasive treatment. However, further height loss post-PVP prompts investigation into contributing factors.AimTo investigate the factors associated with further height loss following PVP with cement augmentation in OVCF patients.MethodsA total of 200 OVCF patients who underwent successful PVP between January 2021 and December 2022 were included in this study. "Further height loss" during 1 year of f

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… MC-modified PMMA bone cement (Mendec Spine; Tecres S.P.A., Verona, Italy) consisted of 22.6 g conventional bone cement, 3.4 g MC (Beijing Allgens Medical Science and Technology Co., Ltd., Beijing, China), and 10 mL MMA monomer liquid, which were thoroughly mixed to achieve the required viscosity. …

Оценка продолжительного протеинового ингибирования с помощью перорального режима на основе ixazomib на недавно диагностированном и впервые возвращенном миеломе: исследование реальных китайских групп.

ЦЕЛЬ: Оценка эффективности, безопасности и удобства перехода в классе (ICT) от инъекционного инъекции на основе бортезомиба к пероральным режимам на основе ixazomib. МЕТОДЫ: Это ретроспективное исследование реального мира было проведено в 16 Китайские больницы в период с октября 2017 и апрель 2023 и проанализировали вновь диагностированные (NDMM) и перинатальные рецидивы нескольких пациентов миэломы (FRMM), которые получили по крайней мере частичный ответ от вводного индукционного лечения на основе бортезомиба, за которым последовал оральный режим на основе ixazomib 2 год или до развития болез

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… The study protocol was conducted in accordance with the principles of the Declaration of Helsinki and approved by the Health Human Research Ethics Committee of Beijing Chaoyang Hospital, Affiliated to Capital Medical University (approval no. 2019‐327). … ETHICAL APPROVAL The study protocol was conducted in accordance with the principles of the Declaration of Helsinki and approved by the Health Human Research Ethics Committee of Beijing Chaoyang Hospital, Affiliated to Capital Medical University (approval no.2019‐327). …

Диагностический потенциал двух новых биомаркеров для спектра нейромиелита оптики и множественной склероза

Фон: В настоящее время нет тестов, которые могут однозначно диагностировать и различать нейромиелит оптического спектра (NMOSD) от множественной скlerоза (МС). Методы: Сначала были использованы протеомные исследования спинно-мозговой жидкости (ССФ) для выявления новых биомаркеров, которые различают NMOSD от МС в группах из 10 пациентов с МС и 10 пациентов с NMOSD. Далее биомаркеры были проверены с помощью метода связывания иммунно-электрофоретической реакции и образцов ССФ и сыворотки от 20 пациентов с МС, 20 пациентов с NMOSD, 20 непатологических нейрологических контролей и 20 здоровых контро

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… Patients From 2018 to 2021, a number of individuals registered at the Beijing Tiantan Hospital of Capital Medical University and 100 were enrolled in this study. The research consisted of two phases. Initially, potential biomarkers were found using discovery samples of 10 MS and 10 NMOSD patients. … Institutional Review Board Statement The study was conducted in accordance with the Declaration of Helsinki and approved by the Institutional Review Board (or Ethics Committee) of Beijing Tiantan Hospital of Capital Medical University (protocol code KYSQ 2020-092-01, approved on 10 March 2020). …

Peripheral inflammatory T cell subsets are effective predictive factors in the development of heterotopic ossification after posttraumatic elbow surgery.

Heterotopic ossification refers to the pathological formation of extra-skeletal bone. It is a common complication of trauma or surgery that can cause disability and has no definitive cure. Furthermore, the mechanisms underlying chronic inflammation during ossification remain unclear. Therefore, this study aimed to elucidate the systemic immune microenvironment status of heterotopic ossification and identify biomarkers of therapeutic efficacy and recurrence. A combination of stereoarthrolysis with prophylactic radiotherapy and non-steroidal anti-inflammatory drugs was used to treat patients wit

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… The raw sequence data reported in this paper have been deposited in the Genome Sequence Archive (Genomics, Proteomics & Bioinformatics 2021) at the National Genomics Data Center (Nucleic Acids Res 2022), China National Center for Bioinformation/Beijing Institute of Genomics, Chinese Academy of Sciences … The following primer sequences were used (Tsingke Biotechnology, Beijing, China): OCN (forward: 5′-GCAGCTTGGTGCACACCTAG-3′, reverse: 5′-ACCTTATTGCCCTCCTGCTT-3′) and GAPDH (forward: 5′-GAAGGTGAAGGTCGGAGTC-3′, reverse: 5′-GAAGATGGTGATGGGATTTC-3′). …

Sufficiently activated mature natural killer cells derived from peripheral blood mononuclear cells substantially enhance antitumor activity.

BACKGROUND: Peripheral blood-derived natural killer (NK) cells spontaneously lyse tumor cells without prior sensitization. However, NK cells in peripheral blood (PBNK cells) are in a resting state and exhibit inhibitory phenotypes and impaired cytotoxicity. Thus, strengthening the cytotoxic effector function of PBNK cells and improving NK cell expansion in vitro for a convenient allogeneic therapy are essential. MATERIALS AND METHODS: Pure cytokine activation and expansion of NK cells (super NK [SNK]) from peripheral blood mononuclear cells were studied. Markers of activated and inhibited NK

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… The mice were housed in accordance with the requirements for specific pathogen‐free‐grade animals (SYXK [Beijing] 2019‐0028) in standard cages ( n = 6 per cage) at 23 ± 3°C on a 12/12 h light–dark cycle in a clean room with humidity levels ranging from 40% to 70%. … Ex vivo NK cell expansion protocol Sufficiently activated mature NK cells (super NK [SNK]) were expanded from healthy donor PBMC using a DCTY NK serum‐free medium culture kit (LY1201; Beijing DCTY Biotech Co., Ltd). …

Prognostic nomogram for multiple myeloma early relapse after autologous stem cell transplant in the novel agent era.

BackgroundThe present study intended to establish a predictive nomogram for early relapse (ER) (Patients and methodsThe nomogram was designed and constructed to a retrospective clinical data of newly diagnosed MM patients received novel agent induction therapy and subsequent ASCT at three centers in China from July 2007 to December 2018. The retrospective study was conducted among 294 patients in the training cohort and 126 in the validation cohort. The nomogram's predictive accuracy was evaluated by the concordance index, calibration curve and decision clinical curve.ResultsThe study cohort i

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… The Ethics Committee of Beijing Chao‐Yang Hospital approved this study following the Declaration of Helsinki. … ETHICAL APPROVAL The Ethics Committee of Beijing Chao‐Yang Hospital approved this study following the Declaration of Helsinki. Supporting information Data S1 Click here for additional data file. …

Novel Small Molecular Compound AE-848 Potently Induces Human Multiple Myeloma Cell Apoptosis by Modulating the NF-κB and PI3K/Akt/mTOR Signaling Pathways.

BackgroundWe aimed to investigate the anti-multiple myeloma (MM) activity of the new small molecular compound AE-848 (5-bromo-2-hydroxyisophthalaldehyde bis[(1-methyl-1H-benzimidazol-2-yl)hydrazone]) and its underlying anti-MM mechanism.MethodsCell viability and apoptosis were detected and quantified by using MTT and flow cytometry, respectively. JC-1 dye-related techniques were used to assess mitochondrial membrane potential (MMP). Western blotting was applied to detect the expression of NF-κB and PI3K/Akt/mTOR pathway-associated proteins. The in vivo activity of AE-848 against MM was evaluat

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… MTT was obtained from Solarbio (Beijing, China). … The study obeyed the principles of the ethical guidelines outlined by the International Council for Laboratory Animal Science (ICLAS). 15 Female NOD/SCID mice (6–8 weeks, weighing 18–20 g) were purchased from the Beijing Vital River Laboratory Animal Technology Co., Ltd (Beijing, China) and raised under …

Current landscape of innovative drug development and regulatory support in China.

The global pharmaceutical landscape remains dynamic and competitive, shaped by advancements in first-in-class therapies and breakthrough technologies. The United States has maintained its leadership in first-in-class therapies and breakthrough technologies, driven by advanced regulatory pathways, significant multinational corporation investments, a robust Research and Development (R&D) workforce, and continuous technological innovation. Additionally, global impact of the Food and Drug Administration (FDA) is further amplified through collaborations like Project Orbis, which facilitates simulta

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… TCM Innovative Drugs Ramulus Mori (Sangzhi) alkaloids tablets 03/27/2020 Beijing Wehand-Bio Type 2 diabetes First anti-diabetic TCM drug approved in the last decade. … Icariin Soft Capsules 01/10/2022 Beijing Shenogen Hepatocellular carcinoma Conditionally approved through the priority review process; Class 1.2 TCM innovative drug. …

Oral Presentations

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Контрольный показатель прогресса в борьбе с раком, обусловленным конкретными причинами, в Китае: общенациональный анализ преждевременной смертности в результате 1990 в адрес 2023.

СПРАВОЧНАЯ ИНФОРМАЦИЯ: Организация Объединенных Наций достигла цели в области устойчивого развития 3.4 (ЦУР) 3.4), цель которого заключается в сокращении за счет one- третья ранняя смертность от неинфекционных заболеваний, включая рак, 2030. Однако все еще не проведена всеобъемлющая оценка исторических тенденций и прогнозов преждевременной смертности от рака на национальном, региональном уровнях и по типам рака. Мы провели оценку преждевременной смертности в разбивке по полу, возрасту и возрасту. 33 Провинции Китая с использованием результатов глобального исследования бремени болезней 2023. Ра

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… North: Beijing, Tianjin, Hebei, Inner Mongolia, Shanxi; Northeast: Heilongjiang, Jilin, Liaoning; East: Anhui, Fujian, Jiangsu, Jiangxi, Shandong, Shanghai, Zhejiang; South: Guangdong, Guangxi, Hainan, Hong Kong, Macao; Central: Hubei, Hunan, Henan; Northwest: Ningxia, Qinghai, Shaanxi, Xinjiang, Gansu … North: Beijing, Tianjin, Hebei, Inner Mongolia, Shanxi; Northeast: Heilongjiang, Jilin, Liaoning; East: Anhui, Fujian, Jiangsu, Jiangxi, Shandong, Shanghai, Zhejiang; South: Guangdong, Guangxi, Hainan, Hong Kong, Macao; Central: Hubei, Hunan, Henan; Northwest: Ningxia, Qinghai, Shaanxi, Xinjiang, Gansu …