PLAQUE ASSAY PROCEDURE FOR COLORADO TICK FEVER VIRUS.
Deig, E. Frank (University of California, Berkeley), and H. M. S. Watkins. Plaque assay procedure for Colorado tick fever virus. J. Bacteriol. 88:42-47. 1964.-A reproducible plaque assay procedure is described for the quantitation of Colorado tick fever virus in a cell line established from embryonic hamster tissue. Under the best conditions, plaques approximately 4 mm in diameter were formed after incubation at 37 C of 4 to 6 days. Several environmental variables in the procedure were studied. Efficiency was increased markedly by combining the virus during adsorption with serum proteins, and …
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