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Оценка вируцидальной эффективности пероксида водорода с точки зрения воздействия вируса желтой лихорадки 17-ДДД для применения в отрасли по производству вакцин.

Цель этого исследования состояла в том, чтобы оценить эффективность ин активации пероксида водорода к штамму вакцины от вируса желтой лихорадки 17-ДДД, используемой для производства вакцины от пониженной желтой лихорадки, в двух матрицах: вакцины от желтой лихорадки (ФГВ) и вирусной подвески желтой лихорадки - активный фармацевтический ингредиент (АПИ). Образцы были высушены на нержавеющей стали и подвергались воздействию жидкости пероксида водорода (HPL) в концентрациях 30, 10, 3 и 1% в течение 20 и 60 минут; и паров пероксида водорода (HPV) в изоляторе. В результате воздействия HPL 30% и 10%

Совпадение в аннотации оригинала
The aim of this study was to evaluate the inactivation performance of hydrogen peroxide to the yellow fever virus 17DD vaccine strain, used for the production of attenuated yellow fever vaccine, in two matrixes: formulated yellow fever vaccine (FYV) and yellow fever viral suspension - active pharmaceutical ingredient (API). The samples were dried on stainles…

An Inhibitor of the Sodium-Hydrogen Exchanger-1 (NHE-1), Amiloride, Reduced Zinc Accumulation and Hippocampal Neuronal Death after Ischemia.

Acidosis in the brain plays an important role in neuronal injury and is a common feature of several neurological diseases. It has been reported that the sodium-hydrogen exchanger-1 (NHE-1) is a key mediator of acidosis-induced neuronal injury. It modulates the concentration of intra- and extra-cellular sodium and hydrogen ions. During the ischemic state, excessive sodium ions enter neurons and inappropriately activate the sodium-calcium exchanger (NCX). Zinc can also enter neurons through voltage-gated calcium channels and NCX. Here, we tested the hypothesis that zinc enters the intracellular …

Effects of hydrogen-rich water prepared by alternating-current-electrolysis on antioxidant activity, DNA oxidative injuries, and diabetes-related markers.

Hydrogen-rich water is conventionally prepared by direct current-electrolysis, but has been not or scarcely prepared by alternating current (AC)-electrolysis. The AC preparations from tap water for 20-30 minutes exhibit a dissolved hydrogen concentration of 1.55 mg/L, which was close to the theoretical maximum value of 1.6 mg/L. These preparations also displayed an oxidation-reduction potential of -270 mV (tap water: +576 mV) and pH of 7.7-7.8, being closer to physiological values of body fluids than general types of direct current-electrolytic hydrogen-rich water. We examined whether AC-elect…

Carnitine, apelin and resveratrol regulate mitochondrial quality control (QC) related proteins and ameliorate acute kidney injury: role of hydrogen peroxide.

Mitochondrial impairment is recognised as a prominent feature in kidney diseases. Therefore, we investigated whether the effects of resveratrol, L-carnitine, and apelin in the acute kidney injury model were associated with modulation of mitochondrial quality control (QC) related proteins, intra-renal renin-angiotensin (RAS) activity, adenosine triphosphate (ATP) and Na+-K+ ATPase gene expression. Rats were randomly assigned to 7 groups: Distilled water injected control group, DMSO injected control group, distilled water injected lipopolysaccharide (LPS) group, DMSO injected LPS group, resverat

Self-healing gelatin-based shape memory hydrogels via quadruple hydrogen bonding and coordination crosslinking for controlled delivery of 5-fluorouracil.

Gelatin-UPy based on gelatin with ureidopyrimidinone (UPy) side chains was prepared with varying content of UPy units. On increasing the UPy content, the glass transition temperature, crystallinity and swelling decreased. Gelatin-UPy demonstrated self-healing properties as the UPy units could reversibly form dimers. At the same time, the gelatin-UPy and gelatin-UPy hydrogels demonstrated thermal responsive shape memory behaviors. The introduction of coordination crosslinking by introducing Fe3+ in gelatin-UPy hydrogels not only enhanced the crosslinking degree of gelatin-UPy and decreased the

Exogenous Hydrogen Sulfide Regulates Mycoplasma pneumoniae Lipid-Associated Membrane Proteins to Induce Expression of Heme Oxygenase-1 and Proinflammatory Cytokines.

This study was designed to investigate the effect of exogenous hydrogen sulfide (H2S) on the secretion of Heme oxygenase (HO-1) and proinflammatory cytokines in human mononuclear cell line THP-1 stimulated by lipid-associated membrane proteins (LAMPs) prepared from Mycoplasma pneumoniae (M. pneumoniae) and explore its regulatory mechanism. Cultured cells were stimulated with M. pneumoniae LAMPs after pretreatment with H2S to analyze the production of proinflammatory cytokines and HO-1 by enzyme-linked immunosorbent assay (ELISA) and Western blot. The results showed that THP-1 cells, which were…

A highly sensitive and adjustable colorimetric assay of hydrogen sulfide by signal amplification based on G-quadruplex-Cu2+ peroxidase mimetics.

This work reports the first example of a colorimetric H2S sensor constructed through G-quadruplex-Cu2+ (G4-Cu2+) peroxidase mimetics employing Cu2+ ions and G-rich DNA with signal amplification. In the hydrogen peroxide (H2O2)-mediated oxidation of 3,3',5,5'-tetramethylbenzidine (TMB), the catalytic capacity of Cu2+ can be greatly improved in the presence of 22AG DNA, where 22AG DNA acts as a signal amplifier. However, G4-Cu2+ peroxidase mimetics lose their catalytic abilities after reacting with H2S. This is employed to develop a colorimetric assay of H2S without complex synthesis and instrum…

Fluorinated ZnFeIII Hollow Metal-Organic Framework as a 19F NMR Probe for Highly Sensitive and Selective Detection of Hydrogen Sulfide.

Hydrogen sulfide (H2S) is considered as a highly toxic environmental pollutant and an important signal transmitter in physiological processes, and the selective and reliable detection of H2S is of great concern and remains challenging. Herein, we report a smart sensitive "off-on" 19F NMR sensor for H2S by partially introducing a fluorinated ligand to construct a hollow dual metal-organic framework (MOF) nanosystem, F-ZnFeIII hMOF, in which the fluorinated ligand acts as the 19F signal source but is initially quenched due to the strong paramagnetic relaxation enhancement (PRE) effect from neigh…

Enhancing Enzyme-like Activities of Prussian Blue Analog Nanocages by Molybdenum Doping: Toward Cytoprotecting and Online Optical Hydrogen Sulfide Monitoring.

Artificial nanozymes have been designed to solve the problems of high cost and poor stability involving natural enzymes in analytical applic … vity of Nanocages was integrated with an online sensing platform for in vivo and continuous optical hydrogen sulfide monitoring in the brains of living rats. Our findings may provide possibilities for advancing the design strategy of highly active nanozymes as well as nanozyme-based in vivo detection methods and will offer unique opportunities for their in …

Maternal N-Acetylcysteine Therapy Prevents Hypertension in Spontaneously Hypertensive Rat Offspring: Implications of Hydrogen Sulfide-Generating Pathway and Gut Microbiota.

Hypertension can come from early life. N-acetylcysteine (NAC), a hydrogen sulfide (H2S) precursor as well as an antioxidant, has antihypertensive effect. We investigated whether maternal NAC therapy can protect spontaneously hypertensive rats (SHR) male offspring against hypertension. The pregnant rats were assigned to four groups: SHRs without treatment; Wistar Kyoto (WKY) without treatment; SHR+NAC, SHRs received 1% NAC in drinking water throughout pregnancy and lactation; and, WKY+NAC, WKY rats received 1% NAC in drinking water during pregnancy and lactation. Male offspring (n = 8/group) we…

Hydrogen sulfide induces Ca2+ release from the endoplasmic reticulum and suppresses ATP-induced Ca2+ signaling in rat spinal cord astrocytes.

Hydrogen sulfide (H2S) has a variety of physiological functions. H2S reportedly increases intracellular Ca2+ concentration ([Ca2+]i) in astrocytes. However, the precise mechanism and functional role of this increase are not known. Here, we examined the effects of H2S on [Ca2+]i in astrocytes from the rat spinal cord and whether H2S affects ATP-induced Ca2+ signaling, which is known to be involved in synaptic function. Na2S (150 μM), an H2S donor, produced a nontoxic increase in [Ca2+]i. The [Ca2+]i increase by Na2S was inhibited by Ca2+ depletion in the endoplasmic reticulum (ER) but not by re…

Ultra-small molybdenum sulfide nanodot-coupled graphitic carbon nitride nanosheets: Trifunctional ammonium tetrathiomolybdate-assisted synthesis and high photocatalytic hydrogen evolution.

The preparation of nanoscale molybdenum sulfide (MoS2)-modified graphitic carbon nitride (g-C3N4) nanosheets usually contains complex and mu … ination, except for the promoting generation of the g-C3N4 nanosheets by produced ammonia (NH3) and hydrogen sulfide (H2S) gases, the above (NH4)2MoS4 decomposition not only can efficiently clip the s-heptazine framework to produce more terminal amino groups and cyano groups, but also can produce ultra-small MoSx nanodots on the resultant g-C3N4 nanosheet …

Hydrogen sulfide inhibits aortic valve calcification in heart via regulating RUNX2 by NF-κB, a link between inflammation and mineralization.

INTRODUCTION: Hydrogen sulfide (H2S) was revealed to inhibit aortic valve calcification and inflammation was implicated in the pathogenesis of calcific aortic valve disease (CAVD). OBJECTIVES: We investigate whether H2S inhibits mineralization via abolishing inflammation. METHODS AND RESULTS: Expression of pro-inflammatory cytokines, interleukin-1β (IL-1β) and tumor necrosis factor α (TNF-α) were increased in patients with CAVD and in calcified aortic valve of ApoE-/- mice. Administration of H2 2S releasing donor (4-methoxyphenyl piperidinylphosphinodithioc acid (AP72)) exhibited inhibition …

Exogenous hydrogen sulfide inhibits neutrophils extracellular traps formation via the HMGB1/TLR4/p-38 MAPK/ROS axis in hyperhomocysteinemia rats.

Hydrogen sulfide (H2S) prevents platelet activation and neutrophils extracellular traps (NETs) formation. However, the mechanism of sodium hydrosulfide (NaHS, a donor that produces H2S) inhibits the formation of NETs in hyperhomocysteinemia (HHcy) rats has not been previously investigated. In the experiment, the expressions of HMGB1 of platelets, the expressions of TLR4, PAD4 and the phosphor-p38 of neutrophils were measured. The NETs formations, the concentration of DNA in the serum and the culture solution of cultured neutrophils which was stimulated by platelet-rich plasma (PRP) were tested…

Novel hydrogen-producing Si-based agent reduces oxidative stress, and improves sperm motility and in vitro fertilization rate in varicocoele.

BACKGROUND: Varicocoele-induced male infertility potentially involves oxidative stress. Although varicocoelectomy is recommended for varicocoele patients presenting abnormal semen findings, no pharmacotherapeutic methods currently exist. We have recently developed a silicon-based agent that produces hydrogen by the reaction with water. OBJECTIVES: This study aimed to investigate the therapeutic effects of oral administration of a Si-based agent on varicocoele rat. MATERIALS AND METHODS: Twenty-one rats were divided into four groups: varicocoele + normal diet (n = 5), varicocoele + Si-based a…

Highly sensitive and simple colorimetric assay of hydrogen peroxide and glucose in human serum via the smart synergistic catalytic mechanism.

Due to the own defects of natural enzymes, artificial simulated enzymes are always concerned. Here, the fabricated graphene oxide (GO)/AuNPs nanocomposite exhibits strong synergistic catalysis of peroxidase-mimicking enzymes in combination with the novel property of GO catalytic interface and AuNPs-mediated electron transfer. It can efficiently catalyze the oxidation of enzyme substrate TMB by hydrogen peroxide to form blue TMB oxide. Based on this, the rapid and highly sensitive colorimetric detection of hydrogen peroxide was achieved. Because of the wonderfully synergistic coupling catalysis…

Effects of hydrogen gas production, trapping and bubble-facilitated transport during nanoscale zero-valent iron (nZVI) injection in porous media.

The injection of nanoscale zero-valent iron (nZVI) can be an effective technique for the treatment of groundwater contaminants, including chlorinated solvents. However, its effectiveness can be limited by natural reductant demand (NRD) reactions, including the reduction of water resulting in the production of hydrogen gas. This study presents results from a series of laboratory experiments to investigate gas production and mobilization following the injection of nZVI solutions, along with sodium borohydride (NaBH4) that is used for nZVI synthesis. Experiments were performed in a thin, two-dime…

One-Pot Hydrogen Cyanide-Based Prebiotic Synthesis of Canonical Nucleobases and Glycine Initiated by High-Velocity Impacts on Early Earth.

Chemical environments of young planets are assumed to be significantly influenced by impacts of bodies lingering after the dissolution of th … osphere in the presence of montmorillonite. This one-pot synthesis begins with de novo formation of hydrogen cyanide (HCN) and proceeds through intermediates such as cyanoacetylene and urea.

Performance and microbiological safety testing after multiple use cycles and hydrogen peroxide sterilization of a 5-mm vessel-sealing device.

OBJECTIVE: To evaluate the functional and microbiological safety of a single-use laparoscopic vessel-sealing device in a multiuse setting. … pressure testing of sealed porcine carotid arteries. The handsets were cleaned and sterilized with hydrogen peroxide gas and repackaged. RESULTS: Mean ± SD cycles to failure was 7.7 ± 2.8, with a minimum of 4 and a maximum of 12 use cycles achieved. Eleven of the 12 handsets failed by failure of handset activation after depression of the activation trigg …

Impact of copper sulphate, potassium permanganate, and hydrogen peroxide on Pseudanabaena galeata cell integrity, release and degradation of 2-methylisoborneol.

Frequent off-flavor events caused by geosmin and 2-methylisoborneol have caused concern among consumers about the quality of potable water. Pseudanabaena galeata, a filamentous cyanobacterium, is a known producer of 2-methylisoborneol in lakes and reservoirs. The use of algicides to control cyanobacteria must consider the potential release of contaminants into the water. This is the first study to systematically investigate the effectiveness of copper sulphate (CuSO4), potassium permanganate (KMnO4), and hydrogen peroxide (H2O2) on the cell viability and integrity of Pseudanabaena galeata. Fol…