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Breast calcifications on mammography from systemic amyloidosis: A case report.

Calcifications on mammography from systemic disease at times meet diagnostic criteria for histologic sampling to exclude malignancy. We present a case of bilateral groups of new calcifications on mammography that yielded amyloidosis on core biopsy. Awareness of our patient's known diagnosis of systemic light chain amyloidosis (AL) prompted use of Congo red staining to confirm the histologic diagnosis. Knowledge of systemic diseases with possible manifestations on mammography can facilitate cogent and clinically relevant radiology-pathology correlation.

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… Fig 2 Multiple tissue cores were obtained from a single site of biopsy in the right and left breast using digital breast tomosynthesis with a 9 gauge vacuum assisted device. Specimen radiography confirmed the presence of calcifications in the core samples for each breast ( Fig. 3 ). … A single specimen radiograph from each breast confirms the presence of representative calcifications in the acquired tissue samples from digital breast tomosynthesis guided vacuum assisted core needle biopsy. …

Implementing a fully computed tomography-free online adaptive palliative radiotherapy: a one-visit workflow.

Background and purposeSame-day palliative radiotherapy requires rapid workflows, but conventional computed tomography (CT)-based workflows cause delays and strain resources. Advances in cone-beam CT (CBCT) enabled accurate dose calculation and planning without a planning CT. This study evaluated the feasibility and efficiency of a fully CT-free online adaptive workflow for same-day palliative radiotherapy using high-quality CBCT.Methods and materialsThis prospective study enrolled sixteen patients between January-May 2025, of whom fifteen completed same-day treatment. Eligible patients were re

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… Setup was performed using either a deflated vacuum mattress or a 5 cm foam mattress to ensure stability and patient’s comfort. Treatments were delivered using an Ethos LINAC equipped with a HyperSight CBCT (HS-CBCT) imaging panel (Varian). … From patient 12 onwards, a 5 cm foam mattress was used instead of a vacuum mattress. Discussion This study demonstrated the feasibility of delivering fully CT-free treatments in 15 cases, with a median in-room time of 28 min. Two-thirds of patients spent ≤1.5 h in the department. …

Update on cutaneous calciphylaxis.

Calciphylaxis is a devastating disorder with a mortality rate of 80% due to sepsis and organ failure. Hallmarks of this rare disease are arteriolar media calcification, thrombotic cutaneous ischemia, and necrotic ulcerations. Different mechanisms of vascular calcification can lead to calciphylaxis. Early diagnosis by deep cutaneous ulcer biopsy is most important for prognosis. Here, dermatologists play a significant role although treatment usually needs an interdisciplinary approach. Surgical procedures had been the cornerstone of treatment in the past including parathyroidectomy, but recently

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… Vacuum assistedxs closure device improves the take of mesh grafts in chronic leg ulcer patients. Dermatology. 2008;216:250–6. doi: 10.1159/000112937. 57. Emohare O, Kowal-Vern A, Wiley D, Latenser BA. Vacuum-assisted closure use in calciphylaxis. …

Proteomic analysis identifies pathways related to immune dysregulation in patients with hematologic malignancies after COVID-19 infection.

Patients with hematologic malignancies (HMs) are particularly vulnerable to coronavirus disease 2019 (COVID-19) because of underlying immune dysfunction and treatment-related immunosuppression. However, proteomic features associated with different clinical trajectories in this population remain insufficiently characterized. We performed serum proteomic analysis in 40 HM patients with COVID-19 and 15 healthy controls. Compared with controls, HM patients showed impaired immune-related responses during the acute phase of COVID-19. Acute-phase proteomic patterns differed across outcome groups; how

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… The resulting precipitate was washed three times with acetone and dried using a vacuum concentrator (Labconco, USA). … The desalted peptides were vacuum-centrifuged to dryness, then reconstituted in Milli-Q water containing 0.1% formic acid (FA) for liquid chromatography-mass spectrometry (LC-MS) analysis. …

Advances in MALDI Mass Spectrometry Imaging Single Cell and Tissues.

Compared with conventional optical microscopy techniques, mass spectrometry imaging (MSI) or imaging mass spectrometry (IMS) is a powerful, label-free analytical technique, which can sensitively and simultaneously detect, quantify, and map hundreds of biomolecules, such as peptides, proteins, lipid, and other organic compounds in cells and tissues. So far, although several soft ionization techniques, such as desorption electrospray ionization (DESI) and secondary ion mass spectrometry (SIMS) have been used for imaging biomolecules, matrix-assisted laser desorption/ionization (MALDI) is still t

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… The ideal matrix generally has the following properties: strong electron absorption at the adopted laser wavelength, better vacuum stability, lower vapor pressure, and better miscibility with the analyte in the solid state ( Calvano et al., 2018 ). … Compared with solid matrices, ILM has good vacuum stability, good reproducibility, and high sensitivity ( Armstrong et al., 2001 ). For example, the analysis of lipid can be enhanced by applying ILM in MALDI. …

Сексуальное здоровье после неврологических расстройств: всеобъемлющий обзор данных о лечении.

Предыстория/задачи: Сексуальная дисфункция (СД) и более широкие проблемы сексуального здоровья являются распространенными после неврологических расстройств, однако улики вмешательства разрознены по различным условиям и результатам. В ходе этого зонтичного обзора были собраны и оценены на систематической основе данные о мерах, направленных на УР и сексуальное здоровье неврологических групп населения, а также квалифицированные выводы с использованием достоверной информации. Методы: с момента создания были проведены обыски PubMed, Web of Science, Cochrane Bibrary, Embase, PsycINFO, EBCOCOhost и S

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… Pharmacological interventions, especially PDE5 inhibitors, dominated the literature, with additional data on intracavernosal injections, vacuum erection devices, penile prostheses, testosterone replacement, and neuromodulatory or stimulation-based techniques [ 48 , 49 , 54 , 55 , 56 , 64 , 67 , 68 , … Intracavernosal injections with papaverine, papaverine plus phentolamine, or prostaglandin E1, as well as vacuum erection devices and penile prostheses, achieved high rates of erections sufficient for intercourse in case series and nonrandomized comparative studies, with response rates frequently above …

Frentizole, a Nontoxic Immunosuppressive Drug, and Its Analogs Display Antitumor Activity via Tubulin Inhibition.

Antimitotic agents are one of the more successful types of anticancer drugs, but they suffer from toxicity and resistance. The application of approved drugs to new indications (i.e., drug repurposing) is a promising strategy for the development of new drugs. It relies on finding pattern similarities: drug effects to other drugs or conditions, similar toxicities, or structural similarity. Here, we recursively searched a database of approved drugs for structural similarity to several antimitotic agents binding to a specific site of tubulin, with the expectation of finding structures that could f

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… The reaction mixture was then poured onto cold water and washed with brine, and the organic layer dried over anhydrous Na 2 SO 4 , filtered, and evaporated under vacuum. … The reaction mixture was poured onto ice washed with 1N HCl, 5% NaHCO 3 , and brine, and the organic layer was dried over anhydrous Na 2 SO 4 , filtered, and evaporated under vacuum. 4.1.3. …

The Italian Society of Andrology and Sexual Medicine (SIAMS), along with ten other Italian Scientific Societies, guidelines on the diagnosis and management of erectile dysfunction.

PURPOSE: Erectile dysfunction (ED) is one of the most prevalent male sexual dysfunctions. ED has been in the past mistakenly considered a purely psycho-sexological symptom by patients and doctors. However, an ever-growing body of evidence supporting the role of several organic factors in the pathophysiological mechanisms underlying ED has been recognized. METHODS: The Italian Society of Andrology and Sexual Medicine (SIAMS) commissioned an expert task force involving several other National Societies to provide an updated guideline on the diagnosis and management of ED. Derived recommendations

Symptom burden and health-related quality of life impacts of smoldering multiple myeloma: the patient perspective.

Abstract Background Smoldering multiple myeloma (SMM) is an early form of multiple myeloma (MM). SMM is typically considered asymptomatic, and research on how it affects health-related quality of life (HRQoL) is limited. This study assessed the symptoms and HRQoL of patients with SMM and those who progressed from SMM to MM and evaluated the content validity of two patient-reported outcome instruments (EORTC QLQ-C30 and nine items from the EORTC QLQ-MY20) for use in SMM clinical trials. To address these objectives, concept elicitation and cognitive interviews were conducted with SMM patients an

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… Fatigue feels like, um, somebody’s just drained me, like somebody did reverse vacuuming, stuck a vacuum in my head and sucked out energy. It – I don’t want to get off the couch, which isn’t my normal personality (68 y.o; Female). …

Combined spatial metabolomics and 4D-DIA quantitative proteomics approaches to explore the relationship between lung cancer and the heart.

Lung cancer and cardiovascular disease pose persistent threats to human health, despite advancements in targeted therapy, percutaneous coronary intervention, and drug treatments. Challenges such as side effects, drug resistance, hospitalization rates, and mortality remain high. These diseases are closely linked, sharing common risk factors and intricately influencing each other. This study aims to investigate the interplay between lung disease and cardiovascular disease by examining changes in cardiac metabolites and protein expression using spatial metabolomics and 4D-DIA quantitative proteom

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… The slides were then dried in a vacuum desiccator for 30 min. … Following digestion, peptides were desalted using a C18 Cartridge, dried with a vacuum concentration meter, concentrated by vacuum centrifugation, and redissolved in 0.1% (v/v) formic acid. …

Vision of Life's Code: Molecular Probe Nanoarchitectonics for Deep RNA/DNA Illumination.

Visualization of RNA and DNA provides a window of opportunity for the early detection of multiple diseases and approaches to manipulate them before any pathological processes occur. However, current imaging systems often fail to meet these demands due to the need for multiple RNA and DNA chemical probes, which increases experimental complexity, reduces awareness of false positives, and limits imaging accuracy in complex biosystems. In this work, we identify the key challenges associated with using multiple probes for RNA and DNA imaging and analyze the mechanisms and performance of existing im

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… . , More advanced ultrahigh-vacuum deposition techniques for macromolecules are still needed. … Similarly, DNA or RNA together with fluorescent molecules can be deposited onto a surface using electrospray techniques and subsequently investigated by STM/AFM under ultrahigh-vacuum conditions. …

Количественная оценка холестерина в мозговой и спинной кордах во время развития, обезвоживания и ремиелинации.

Хлестерин в центральной нервной системе (КНС) в значительной степени неэстерифицирован (>99%) и присутствует главным образом в миелинской оболочке (~70% от общего количества холестерина CNS). Однако во время неврологических заболеваний наблюдалось накопление холестерина. В этом поперечном исследовании мы измерили уровень свободного холестерина и холестерина эстера в мозгу и спинные мозги модели мыши Plp1-iCKO-Myrf во время послеродовой миелиации, демиелинации и ремиелиции с использованием газовой хроматографии-массы спектрометрии с одним ионным мониторингом (GC-MS-SIM) и жидкой хроматографии-м

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… The CHCl 3 layer was vacuum dried (ThermoScientific Savant, Asheville, NC, USA, SPD130DLX). Then, 0.5 mL of CHCl 3 was used to resuspend the lipids and transferred to PTFE-capped glass vials for storage. Finally, the samples were vacuum dried again, weighed, and stored at −80 °C in the freezer. … The reaction vial wall was washed with 300 µL of CHCl 3, and then the CHCl 3 was vacuum evaporated. …

Polybasic nanogels for intracellular co-delivery of paclitaxel and carboplatin: a novel approach to ovarian cancer therapy.

Ovarian cancer is one of the leading causes of cancer-related deaths in women, with limited progress in treatments despite decades of research. Common treatment protocols rely on surgical removal of tumors and chemotherapy drugs, such as paclitaxel and carboplatin, which are capable of reaching cancer cells throughout the body. However, the effectiveness of these drugs is often limited due to toxic reactions in patients, nonspecific drug distribution affecting healthy cells, and the development of treatment resistance. In this study, we introduce a polybasic nanogel system composed of poly(die

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… The intermediate was then dried under vacuum at 40 °C and 28 mmHg overnight before being stored at 4 °C. The purified product was analyzed by proton ( 1 H) and carbon ( 13 C) NMR in CDCl 3 , along with ATR-FTIR spectroscopy for further characterization. … The precipitated product was collected using a vacuum filtration system with a Buchner funnel and then dissolved again in dichloromethane. After a second precipitation step, the product was vacuum-dried overnight and stored at 4 °C. …

Immunomodulatory Потенциал Bibenzil-diгидрофенантрен дериватив изолируется от Calanthe cardioglossa.

Бибензы и дигидрофенантрены демонстрируют многообещающие иммуномудуляторные эффекты в различных заболеваниях человека. В этом исследовании мы изолировали one новая производная дигидрофентена (1), two новые производные бибензил-дигидрофенантрена (2, 3) вместе с 12 известные соединения (4-15) из метанола, выходящего из Calanthe cardioglosa. Эти соединения были выявлены с помощью физико-химических анализов и спектроскопических методов. :: three Новые соединения обладают энантиомерными формами, и их конфигурации были определены путем сопоставления экспериментального электронного дикроза (ЭОД) спек

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… Dry column vacuum chromatography (DCVC) and column chromatography (CC) isolations were carried out on silica gel 60 (Merck, Darmstadt, Germany) with particle sizes of 40–63 and 63–200 μm. Sephadex LH-20 (Merck, Darmstadt, Germany) was used for separation by size exclusion chromatography. … The MeOH extract (74.6 g) was subjected to dry column vacuum chromatography (DCVC) using silica gel with a hexane-acetone gradient to give 7 fractions (Fr.A-G). Fraction E (2.6 g) was isolated with column chromatography (CC) on silica gel (MeOH–CH 2 Cl 2 gradient) to give 7 fractions (EI-EVII). …

Генетические причины непатогенных штаммов Pseudomonas syringae pv. actinidiae в киви-садах.

Бактериальная канцерозная болезнь стала наибольшей угрозой для выращивания и производства киви. Мономорфная подпопуляция Pseudomonas syringae pv. actinidiae biovar 3 (Psa3) отвечает за пандемию по всему миру. В пандемической подпопуляции и других подпопуляциях Psa3, вызывающих эпидемии в Китае, обнаружена разнообразие в патогенности. Однако генетические основы еще не были раскрыты. В данном исследовании 117 изолятов Psa3 были идентифицированы с помощью Psa- и P. Psa3-специфических праймеров и оценены по патогенности. Три изолата G4, G40 и S2 не патогенны для киви и не вызывают гиперчувствитель

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… Pathogenicity Assays and Bacterial Infiltration in N. benthamiana Leaves Pathogenicity tests of the Psa3 isolates were performed according to previously described methods: (1) wound inoculation on detached dormant kiwifruit canes with 10 8 CFU/mL bacteria, (2) vacuum infiltration inoculation on leaf … The pathogenicity patterns of 54 Psa3 isolates from Guizhou Province were determined using a vacuum-infiltration inoculation method on leaf discs of Actinidia chinensis var. chinensis “Hongyang.” …

[Exploration on clinical treatment of incisional wound infection after orthopedic internal fixation for lower extremity joint injuries].

Objective: To explore the clinical treatment of incisional wound infection after orthopedic internal fixation for lower extremity joint inju … fixation treatment were performed. After thorough debridement, wounds were treated with continuous vacuum sealing drainage (VSD), and then direct suture+VSD or flap transplantation were performed according to the incision and its surrounding skin tissue, infection, suture tension, exposure of internal fixation or bone or Achilles tendon tissue. Accordin …

[Application of closed negative pressure irrigation and suction device in the treatment of high perianal abscess].

Objective: To explore the efficacy of closed negative pressure irrigation and suction device (Patent number: Z200780013509.8) in the treatme … psed. Generally, the irrigation was stopped 5 days later or when the drainage was clear. The closed vacuum suction was maintained for 2 additional days, before the wound was sutured. (2) Conventional drainage group: conventional incision and drainage was carried out. The skin was cut at a diameter of 8 to 10 cm at the site of abscess with most obvious fl …

Sensing nitrogen limitation in Corynebacterium glutamicum: the role of glnK and glnD.

Psychosis only rarely occurs in patients with untreated Parkinson's disease. Much more commonly, psychosis is induced by drug therapy for Parkinson's disease and is the strongest known risk factor for nursing home placement. Delusions are less frequent than hallucinations, but are more concerning as they are often paranoid in nature. Treatment begins with a search for correctable infectious, toxic, and metabolic aetiologies. If symptoms persist, anti-Parkinson's disease medications are slowly reduced. However, withdrawal of these drugs usually worsens parkinsonism and is often not tolerated. C

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… Store polymer-coated substrates/chips in a dry environment (vacuum desiccator) for up to 3 months until probe spotting procedure. 2. Preparation of Probe Array: Antibodies, antigens, ss/dsDNA, RNA, etc. Dilute probe(s) to appropriate concentration in desired buffer. …

Early prognosis prediction in acute myeloid and acute lymphoid leukemia patients using cell-free DNA concentration ratios.

Background: Cell-free DNA (cfDNA) is a promising biomarker for disease prediction in many cancers, including acute leukemia (acute myeloid leukemia [AML] and acute lymphoblastic leukemia [ALL]). This study investigated the role of cfDNA in predicting relapse or unfavorable outcomes in acute leukemia patients upon initial diagnosis. Methods: Paired peripheral blood samples of 25 patients with ALL and AML were compared at baseline and induction/follow-up and clinically correlated with clinicopathological and outcome variables according to the risk category. cfDNA was isolated using commercial cf

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… A QIAGEN QIAamp Circulating Nucleic Acid Kit, along with a vacuum pump-based approach, was utilized for the extraction of cfDNA from peripheral blood samples of AML and ALL patients. The tubes were labeled with unique barcodes and stored at 4°C until further processing, avoiding freezing. … The plasma, lysis buffer, and carrier RNA were incubated at 56°C and then added to the QIAamp Mini spin column, which is attached to the vacuum pump for extraction and purification. The cfDNA was eluted in 50–100 µL using the provided elution buffer. …

Dental status and oxidative homeostasis state in patients exposed to occupational vibration: reduced glutathione content in oral fluid.

AIM: The aim of the research involved determination of reduced glutathione content in oral fluid of patients exposed to occupational vibrati … ontrols (during medical checkups) using the following indices: PMA, OHI-S, DMFT, with assessment of vacuum-pressory resistance of gingival capillaries (VPRC) (according to V. I. Kulazhenko) and community periodontal index of treatment needs (CPITN). RG content (activity) in OF was determined according to Garishvili. Primary data were statistically proces …