Результаты поиска

Improved oncolytic and immunostimulatory activity of the spontaneous jin-3 reovirus mutant in preclinical bladder cancer models.

Immunotherapy has emerged as a promising strategy for treatment of urothelial carcinoma of the bladder (UCB) but not all patients show clinically desirable responses upon treatment with immune-checkpoint inhibition. Oncolytic viruses may unleash the full potential of immunotherapy. Besides tumor-lysing properties, oncolytic viruses can induce durable and systemic anti-viral and anti-tumor immune responses. Here, we evaluated and compared oncolytic and immunostimulatory properties of wild-type reovirus (R124) and the junction-adhesion molecule-independent mutant reovirus (jin-3) in preclinical

Совпадение в полном тексте источника
… Tumor Evolution and Drug Response in Patient-Derived Organoid Models of Bladder Cancer Cell 173 2018 515 528.e17 29625057 10.1016/j.cell.2018.03.017 PMC5890941 54 van de Merbel A.F. van der Horst G. van der Mark M.H. van Uhm J.I.M. van Gennep E.J. Kloen P. Beimers L. … Exploring Tumor-Immune Interactions in Co-Culture Models of T Cells and Tumor Organoids Derived from Patients Int. J. Mol. Sci. 24 2023 14609 10.3390/ijms241914609 PMC10572813 37834057 64 Gujar S.A. Pan D.A. Marcato P. Garant K.A. Lee P.W.K. …

Familial Cerebral Cavernous Malformations: Pathophysiology, Genetics, Biomarkers, and Treatment Perspectives.

Familial cerebral cavernous malformations (FCCM) are a heritable neurovascular disorder defined by clusters of dilated, thin-walled capillaries in the brain and spinal cord. Although rare, FCCM offers a tractable model for understanding how genetic disruptions in endothelial junction biology, mechanotransduction, and kinase signaling drive vascular instability in the central nervous system. Pathogenic loss-of-function variants converge on signaling abnormalities that promote barrier dysfunction, iron deposition, inflammation, and progressive lesional growth. Clinically, FCCM may manifest with

Совпадение в полном тексте источника
… Using high‐throughput differentiation of blood‐vessel organoids from CCM1 , CCM2 , or CCM3 knockout hiPSCs, both shared and mutation‐specific defects were demonstrated across endothelial, neuronal, mesenchymal, and fibroblast populations (Skowronek et al. 2025 ). … Through notable methodological advances, two groups developed iPSC‐derived human blood–brain barrier (hBBB) assembloids (or organoids) that combine brain and vascular organoids derived from a human embryonic stem cell lineage (Dao et al. 2024 ). …

Chimeric antigen receptor-based cell therapy for treating urological tumors.

Urological tumors represent a significant global health challenge, with conventional therapies often proving insufficient to control disease progression. Recent breakthroughs in cellular immunotherapy, particularly in chimeric antigen receptor (CAR)-T cell, CAR-natural killer cell, and CAR-macrophage therapies, have demonstrated remarkable potential for treating these malignancies. Ongoing research is actively refining CAR-based strategies to enhance their precision in targeting tumor-associated antigens. This review comprehensively summarizes the applications of CAR cell therapy in the follow

Совпадение в полном тексте источника
… Yu et al. [ 36 ] have developed transmembrane glycoprotein mucin 1 (MUC1)-CAR-T cells that showed specific immune cytotoxicity against MUC1-positive BC organoids, indicating the feasibility of using MUC1-CAR-T cells to treat BC. … Patient-derived organoids of bladder cancer recapitulate antigen expression profiles and serve as a personal evaluation model for CAR-T cells in vitro. Clin Transl Immunology 2021;10(2):e1248. 37. Grunewald CM Haist C König C, et al. …

Навигатор биомаркеров человека.

Биомаркеры играют ключевую роль в современных исследованиях медицины, особенно в ранней диагностике заболеваний и персонализированном лечении. Несмотря на то, что предыдущие исследования систематически обзоры маркеров в различных областях заболеваний, интегрированная система, которая связывает основные физиологические системы, охватывает множество органов и охватывает широкий спектр заболеваний, все еще отсутствует. В контексте современных вызовов здоровья, характеризующихся высокой распространенностью хронических заболеваний и широкими сопутствующими заболеваниями, создание панорамной системы

Oleocanthal as a Multifunctional Anti-Cancer Agent: Mechanistic Insights, Advanced Delivery Strategies, and Synergies for Precision Oncology.

Oleocanthal (OC), a secoiridoid phenolic compound exclusive to extra virgin olive oil (EVOO), has emerged as a promising nutraceutical with multifaceted anti-cancer properties. Despite its well-characterized anti-inflammatory and antioxidant effects, the mechanistic breadth and translational potential of OC in oncology remain underexplored and fragmented across the literature. This comprehensive review synthesizes and critically analyzes recent advances in the molecular, pharmacological, and translational landscape of OC's anti-cancer activities, providing an integrative framework to bridge pr

Совпадение в полном тексте источника
… OC reprograms CAF metabolism by downregulating glycolysis (60% reduction in lactate production) and mitochondrial oxidative phosphorylation, as shown in CAF-rich breast cancer organoids [ 30 ]. …

Advances in CRISPR-Cas technology and its applications: revolutionising precision medicine.

CRISPR-Cas (Clustered Regularly Interspaced Short Palindromic Repeats-CRISPR-associated proteins) has undergone marked advancements since its discovery as an adaptive immune system in bacteria and archaea, emerged as a potent gene-editing tool after the successful engineering of its synthetic guide RNA (sgRNA) toward the targeting of specific DNA sequences with high accuracy. Besides its DNA editing ability, further-developed Cas variants can also edit the epigenome, rendering the CRISPR-Cas system a versatile tool for genome and epigenome manipulation and a pioneering force in precision medic

Critical considerations for targeting colorectal liver metastases with nanotechnology.

Colorectal cancer remains a significant cause of morbidity and mortality worldwide. Half of all patients develop liver metastases, presenting unique challenges for their treatment. The shortcomings of conventional chemotherapy has encouraged the use of nanomedicines; the application of nanotechnology in the diagnosis and treatment of disease. In spite of technological improvements in nanotechnology, the complexity of biological systems hinders the prospect of nanomedicines being applied in cancer therapy at the present time. This review highlights current biological barriers and discusses aspe

Совпадение в полном тексте источника
… More recently it was demonstrated that organoids derived from human CRC liver metastases were able to recapitulate additional aspects of the disease (Buzzelli, Ouaret, Brown, Allen, & Muschel, 2018 ). … As CRC metastasis is a multifaceted and heterogeneous disease, merging different organoid models offers the opportunity to study the effect of intratumoral heterogeneity on CRC phenotypes. …

Targeting Tumor Microenvironment for Cancer Therapy.

Cancer development is highly associated to the physiological state of the tumor microenvironment (TME). Despite the existing heterogeneity of tumors from the same or from different anatomical locations, common features can be found in the TME maturation of epithelial-derived tumors. Genetic alterations in tumor cells result in hyperplasia, uncontrolled growth, resistance to apoptosis, and metabolic shift towards anaerobic glycolysis (Warburg effect). These events create hypoxia, oxidative stress and acidosis within the TME triggering an adjustment of the extracellular matrix (ECM), a response

Совпадение в полном тексте источника
… In terms of long-lasting culture time, organoid technology can circumvent the limitation featured by tumor explants. … Recent strategies for studying drug sensitivity and toxicity include the use of tumor biopsies to produce tumor organoids in a “tumor on a chip” microfluidic device for personalized therapy [ 207 , 208 ]. …

2020 ACVIM Forum On Demand Research Abstract Program.

Аннотация не предоставлена источником. Откройте карточку для перехода к публикации.

Совпадение в полном тексте источника
… Concentrations EN05 | Allison O'Kell | Evaluation for Type 1 Diabetes Associated Autoantibodies in Diabetic and Non‐Diabetic Australian Terriers and Samoyeds EN06 | Rebecca Silveston‐Keith | The Efficacy, Sensitivity, and Specificity of a Saliva Glucose Monitoring System for Diabetic Canines EN07 | Eve Tièche | Organoid … Canine Fecal Dysbiosis GI19 | Fabio Teixeira | Comparison of the Fecal Microbiota between Healthy Dogs and Dogs with Diabetes Mellitus GI20 | Katie Tolbert | Understanding the Failure of Oral Acid Suppressants in Cats GI21 | Sichao Mao | Phenotypic and Functional Characterization of Adult Intestinal Organoids …

The role of AI in oncology: present applications and future horizons.

Artificial intelligence (AI) offers a powerful means to accelerate precision oncology by individualizing care in an era of rapidly evolving treatment paradigms. However, there is limited regulatory oversights for safe clinical implementation of AI, and concerns surrounding data bias, ownership, and privacy have hindered broad integration into healthcare practice. In this perspective, we offer a forward-looking roadmap for the dissemination of AI in oncology. We discuss the role for AI in guiding biomarker-driven patient selection for clinical trials and in facilitating both personalized drug s

Совпадение в полном тексте источника
… Develops organoid models for hematological (AML, MM) malignancies. USA Tempus DL Pan-cancer (solid and liquid tumors) Clinical Trial Selection, Drug Selection Partners with academic centers for site activation of oncology trials. … Commercially available for multi-omic sequencing to predict patient treatment response to ICI, generation of tumor organoids. …

Взаимодействие с Мозгом: Как нанотехнология может способствовать.

Соединение искусственных устройств с человеческим мозгом является главной целью нейротехнологий. Однако наше воображение часто ограничивается существующими парадигмами и технологиями. Со временем, наряду с имеющейся технологией, изменились предложения в отношении интерфейсов мозга и автомата. Механические рычаги и кабельные лебедки использовались для перемещения частей мозга в механическую эпоху. Сложное электронное проводка и дистанционное управление возникли в эпоху электронной связи, что в конечном счете привело к созданию компьютерных интерфейсов. Тем не менее, наши мозги настолько сложны,

Совпадение в полном тексте источника
… Neurophysiology has introduced a number of intermediate options, such as cultures of slices of tissue, even from humans 36 or more recently organoids. 37 − 40 In a later section of this article, we highlight potential contributions of nanotechnology (and related fields) for making more realistic model … for Transducing Electrical Signals into an Optical Readout Nanoparticles and Nanomaterials for Transducing Chemical Signals Traversing the Blood–Brain Barrier Challenge of Specific Interfacing Advanced Test Platforms to Model Aspects of the Brain Organotypic Slice Culture of Rodent Hippocampus 3D Organoids …

Indocyanine Green (ICG) and Colorectal Surgery: A Literature Review on Qualitative and Quantitative Methods of Usage.

Background: Due to its many benefits, indocyanine green (ICG) has gained progressive popularity in operating rooms (ORs) globally. This literature review examines its qualitative and quantitative usage in surgical treatment. Method: Relevant terms were searched in five international databases (1. Pubmed, 2. Sciencedirect, 3. Scopus, 4. Oxfordjournals, 5. Reaxys) for a comprehensive literature review. The main benefits of using ICG in colorectal surgery are: intraoperative fluorescence angiography; fluorescence-guided lymph node involvement detection and the sentinel technique; the fluorescent

Совпадение в полном тексте источника
… Organoids can be generated in vitro, and orthotopic transplantation in mouse models of mucosal injury showed that intestinal organoids spontaneously connected and integrated into the injured epithelium, accelerating healing and weight gain. … The feasibility of labelling intestinal organoids with fluorescent dyes and nanoparticles for confocal laser endomicroscopy (CLE) was explored. CLE evaluated organoid homogeneity, durability, cell viability, differentiation, and efficiency. …

Reimagination in art and science: Seeing the familiar in a new light and creating new things that didn't exist before.

Many groundbreaking works in art and science owe their origins to the creative spark ignited by reimagination-the process whereby artists and scientists reinterpret and reframe past and existing knowledge, envisioning it in a new context that reveals new possibilities. Artistic and scientific innovation originating from reimagination brings to mind William Faulkner's famous line, "The past is never dead. It's not even past." [W. Faulkner, Requiem for a Nun (1)].

Совпадение в полном тексте источника
… Table 1 describes six examples of reimagined discoveries in biomedical science, including AlphaFold, GLP-1 for obesity, next-generation sequencing, organoids, drugs for myeloma, and ubiquitination. … Next-generation sequencing | Transformed the single-fragment Sanger sequencing into a massively parallel analysis of millions of DNA fragments, decreasing costs and transforming genomics Organoids | Flat two-dimensional cell cultures recast as three-dimensional structures that mimic organ function Drugs …

Advances in MALDI Mass Spectrometry Imaging Single Cell and Tissues.

Compared with conventional optical microscopy techniques, mass spectrometry imaging (MSI) or imaging mass spectrometry (IMS) is a powerful, label-free analytical technique, which can sensitively and simultaneously detect, quantify, and map hundreds of biomolecules, such as peptides, proteins, lipid, and other organic compounds in cells and tissues. So far, although several soft ionization techniques, such as desorption electrospray ionization (DESI) and secondary ion mass spectrometry (SIMS) have been used for imaging biomolecules, matrix-assisted laser desorption/ionization (MALDI) is still t

Совпадение в полном тексте источника
… Organoids are in vitro models of human development and disease study. They are often thought of as miniature versions of organs, and they often show very precise microscopic anatomical structures. … Blood-brain-barrier Organoids for Investigating the Permeability of Cns Therapeutics. Nat. Protoc. 13, 2827–2843. 10.1038/s41596-018-0066-x Bhandari D. R., Wang Q., Li B., Friedt W., Römpp A., Spengler B., et al. (2018). …

Иммунитет Т-клеток, специфичный для EBV: значение для множественного склероза.

Генетические и экологические факторы совместно определяют подверженность развитию множественных склерозов (МС). Улучшения в разработке эпидемиологических исследований помогли выявить последовательные ассоциации экологического риска, такие, как повышенная подверженность НР после заражения вирусом Эпштейн-Барр (ЭБВ), в то время как биологические механизмы, влияющие на связь между ЕБВ и НР, по-прежнему недостаточно изучены. Увеличение и расширение репертуара антител и клеток иммунной реакции на антигены EBV, особенно на доминирующий антиген CD4+T-клеток EBV 1 (EBNA1) постоянно наблюдается у пацие

Совпадение в полном тексте источника
… In-vitro models based on reprogramming of blood cells into neural cells and neural organoids that can be co-cultured with immune cells become increasingly available. … Six-month cultured cerebral organoids from human ES cells contain matured neural cells . Neurosci Lett . ( 2018 ) 670 : 75 – 82 . doi: 10.1016/j.neulet.2018.01.040 29398520 123 Lancaster MA Knoblich JA . Generation of cerebral organoids from human pluripotent stem cells . …

Abstracts from the 13th International Conference for Healthcare and Medical Students (ICHAMS).

Аннотация не предоставлена источником. Откройте карточку для перехода к публикации.

Use of Real-World Evidence to Drive Drug Development Strategy and Inform Clinical Trial Design.

Interest in real-world data (RWD) and real-world evidence (RWE) to expedite and enrich the development of new biopharmaceutical products has proliferated in recent years, spurred by the 21st Century Cures Act in the United States and similar policy efforts in other countries, willingness by regulators to consider RWE in their decisions, demands from third-party payers, and growing concerns about the limitations of traditional clinical trials. Although much of the recent literature on RWE has focused on potential regulatory uses (e.g., product approvals in oncology or rare diseases based on sin

Совпадение в полном тексте источника
… health records (EHRs), disease or product registries, and patient health surveys. 1 Increasingly, RWD can be linked to more novel types of data, such as genomic (or other “omic”) data from biobanks, biopsies and other pathology tests, diagnostic imaging, social determinants of health (SDoH), cancer organoids … Progress and potential in organoid research. Nat. Rev. Genet. 19, 671–687 (2018). 42. Prasad, M. , Kumar, R. , Buragohain, L. , Kumari, A. & Ghosh, M. Organoid technology: a reliable developmental biology tool for organ‐specific nanotoxicity evaluation. Front. Cell Dev. …

Microbiota-derived metabolites as modulators of cancer immunotherapy response.

The microbiome is a key regulator of host homeostasis and immune activity, in part through the production of metabolites. These microbiota-derived metabolites can modulate both the innate and adaptive immune system, as well as directly target tumour cells, thereby regulating anti-tumour immunity and response to immunotherapy. Here, we describe the current mechanistic knowledge on how these metabolites exert their effects and outline the methodologies used to detect and assess these metabolites. Finally, we summarize microbiota-targeted therapies capable of improving microbial functionality to

Совпадение в полном тексте источника
… Supernatant of bacteria capable of producing HMBPP promoted Vδ2 T cell-mediated killing of patient-derived tumour organoids. … Treating these immune-tumour organoid co-cultures or PDTFs with bacterial supernatant or pure metabolites may help reveal or validate immunomodulatory effects in a human setting. …

Revisiting potential value of antitumor drugs in the treatment of COVID-19.

Since an outbreak started in China in 2019, coronavirus disease 2019 (COVID-19) has rapidly become a worldwide epidemic with high contagiousness and caused mass mortalities of infected cases around the world. Currently, available treatments for COVID-19, including supportive care, respiratory support and antiviral therapy, have shown limited efficacy. Thus, more effective therapeutic modalities are highly warranted. Drug repurposing, as an efficient strategy to explore a potential broader scope of the application of approved drugs beyond their original indications, accelerates the process of d

Совпадение в полном тексте источника
… To overcome the limitations of cell lines, various organoid culture systems which can simulate the process of SARS-CoV-2 infecting different human organs have been, or are being, developed and used to test potential therapies for COVID-19 [ 140 – 142 ]. … Infection of bat and human intestinal organoids by SARS-CoV-2. Nat Med. 2020;26(7):1077–1083. doi: 10.1038/s41591-020-0912-6. 141. Zhao B, Ni C, Gao R, Wang Y, Yang L, Wei J, et al. Recapitulation of SARS-CoV-2 infection and cholangiocyte damage with human liver ductal organoids. …

Developing a pan-cancer research autopsy programme.

AimsRapid procurement of a wide variety of metastatic and primary cancers and normal tissues after death through rapid autopsy opens largely unexplored avenues in cancer research. We describe a high-volume rapid research autopsy programme at a large academic medical centre.MethodsAdvanced-stage cancer patients, most commonly inpatients in palliative care facilities, were approached to participate in a cancer research autopsy programme with the goal of acquiring multidimensionally annotated tissue for cancer research. On death of an enrolled patient, a predetermined notification plan was enacte

Совпадение в полном тексте источника
… In addition, our autopsy programme features high tissue yields for facilitating the development of preclinical models of disease through establishment of xenografts, organoids and cryobanking of samples for immunotherapy-based studies. … Pan-cancer research autopsies facilitate the development of preclinical models of disease through establishment of xenografts, organoids and cryobanking of samples for immunotherapy-based studies. …