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Neonatal intensive care unit exposures reprogram microbiome-metabolome trajectories and modulate host calprotectin in preterm infants: a longitudinal multi-omics study.

Early-life gut microbiota development is critical for orchestrating mucosal barrier function and immune priming, as disruptions in this process can increase susceptibility to life-threatening diseases such as necrotizing enterocolitis (NEC) and sepsis. This longitudinal multi-omics study of 186 preterm infants (<32 weeks of gestation or <1500 g birth weight) explores the impact of early-life exposures in the neonatal intensive care units (NICUs) on gut microbiota, metabolism, and immune responses. We analyzed 1153 stool samples using quantitative microbial profiling, untargeted metabolomics, a…

Distinct metabolome and flux responses in the retinal pigment epithelium to cytokines associated with age-related macular degeneration: comparison of ARPE-19 cells and eyecups.

Age-related macular degeneration (AMD) is associated with chronic inflammation of the retinal pigment epithelium (RPE) and elevated cytokines including TNFα, TGF-β, IL-6, and IL-1β. As a metabolic intermediary supporting aerobic glycolysis in the adjacent photoreceptors, the RPE's metabolic responses to inflammation and the optimal methods to study cytokine-driven metabolic programming remain unclear. We performed a rigorous comparison of ARPE-19 cells and rat eyecup metabolomes, revealing key distinctions. Rat eyecups exhibit higher levels of lactate and palmitate but depleted glutathione and

Machine learning-guided Orbitrap-HRAMS-based metabolomic fingerprinting for geographical origin, variety and tissue specific authentication, and adulteration detection of turmeric and ashwagandha.

The increasing global demand for herbs and spices in food and nutraceutical industries highlights their key functional benefits, including antioxidant and anti-inflammatory properties. Ensuring authenticity and traceability is essential to counteract challenges such as geographical origin (GO) mislabelling and tissue- or variety-specific adulteration, which can undermine product quality and safety. This study employs LC-Orbitrap-MS-based untargeted metabolomics coupled with machine learning to authenticate the GO, variety, and tissue specificity of turmeric (Curcuma longa) and ashwagandha (Wit…

Integrated transcriptome and metabolome provide insight into phenolics and soluble sugar variation in the different varieties of Gastrodia elata Blume from different areas in China.

Gastrodia elata Blume is known for its "medicinal food homology", its chemical components include phenols, glycosides and polysaccharides. In China, the Anhui, Hubei, Guizhou, and Yunnan provinces are the primary G. elata-producing areas, among which Gastrodia elata Bl. f. elata (GR), Gastrodia elata Bl. f. glauca S. Chow (GB), and Gastrodia elata Bl. f. glauca S. Chow and Gastrodia elata Bl. f. elata (GR×GB) are essential varieties. This research employed metabolomics and Ultra Performance Liquid Chromatography (UPLC) to quantify 19 soluble sugars and six phenolic compounds in G. elata from v…

Dynamics of the Epigenome, Microbiome, and Metabolome in Relation to Early Adiposity in the Maternal-Infant Axis: Protocol for a Prospective, Observational Pilot Study in the Spanish NEMO Cohort.

Background: Childhood obesity has reached epidemic levels in developed countries and is an emerging concern in developing regions. Children with excess weight are more likely to maintain this condition over time into adulthood and face a higher risk of developing metabolic disorders such as type 2 diabetes, hypertension, metabolic dysfunction-associated liver disease, and dyslipidemia. Early identification of obesity risk is, therefore, a key public health challenge. Methods: This is an observational, prospective, single-center cohort pilot study in 66 mother-infant dyads recruited at the Gyne

Treatment response variations to a single large bolus of enteral cholecalciferol in vitamin D deficient critically Ill children: Metabolomic insights for precision nutrition.

Vitamin D deficiency (VDD) is prevalent globally and in pediatric intensive care units, where it represents a modifiable risk factor that may impact patient recovery during hospitalization. Herein, we performed a retrospective analysis of serum samples from a phase-II randomized placebo-controlled trial involving a single large bolus of 10,000 IU/kg vitamin D3 ingested by critically ill children with VDD (25-OH-D < 50 nmol/L). Targeted and untargeted methods were used to comprehensively measure 6 vitamin D metabolites, 239 lipids, 68 polar metabolites, and 4 electrolytes using a multi-step dat

Gut microbiota and metabolomic: The potential mechanism underlying the female-specific protective effects of Craterellus cornucopioides polysaccharide against chemotherapy-induced intestinal injury.

Chemotherapy agents, including cyclophosphamide (CTX), frequently induce severe intestinal injury characterized by oxidative stress, inflammatory responses, and microbiota dysbiosis, particularly in females who exhibit heightened immune and metabolic sensitivity. This investigation assessed the protective properties of Craterellus cornucopioides polysaccharide (CCP) against CTX-induced intestinal injury, with a focus on their impact on women's health through modulation of the gut microbiota and metabolic pathways. CCP significantly improved antioxidant and immune biomarkers (sIgA, SOD, CAT, T-

(R)-Bambuterol attenuates DSS-induced chronic colitis by suppressing inflammation, repairing intestinal barrier, and modulating gut microbiota and serum metabolomic profile.

Ulcerative colitis (UC) is a chronic and recurrent intestinal inflammatory disease, owing to its increasing incidence worldwide, UC poses a … uencing revealed that (R)-BMB restored the homeostasis of the intestinal microbiota, and untargeted metabolomics analysis revealed that the changes in the metabolomics profiles were restored after (R)-BMB treatment. In conclusion, (R)-BMB alleviated the mice with DSS-induced chronic colitis via inhibiting the immune response and reshaping the intestinal microb …

Rapid diagnosis of asthma via metabolomic analysis of exhaled volatile organic compounds using high-pressure photoionization time-of-flight mass spectrometry.

Asthma (AS) is a global public health issue driven by chronic airway inflammation, which alters the volatile organic compound (VOC) profile in exhaled breath. This study aimed to achieve rapid, accurate, and non-invasive asthma diagnosis via an untargeted metabolomic analysis of exhaled breath using high-pressure photoionization time-of-flight mass spectrometry (HPPI-TOFMS). Nineteen differential metabolites in exhaled breath were identified by statistical analysis between 21 asthma patients and 21 healthy controls with an analysis time of only 2 min for each sample. An asthma diagnostic model

Urinary metabolome at birth in patients with hypoxic-ischemic encephalopathy treated with therapeutic hypothermia and long-term neurodevelopmental outcomes: a 7-year follow up.

BACKGROUND: Hypoxic-ischemic encephalopathy (HIE) is a leading cause of neonatal mortality and morbidity, yet no validated biomarkers currently exist to predict long-term outcomes. We investigated the potential of the neonatal urinary metabolomic profile as a predictor of long-term neurodevelopmental outcomes in HIE newborns treated with therapeutic hypothermia (TH). METHODS: We conducted a longitudinal study in neonates with HIE undergoing TH. Urine samples collected during TH were analyzed using untargeted metabolomics via mass spectrometry. Based on long-term follow-up outcomes, patients w…

Impact of one-year soy protein supplementation on serum metabolomic profiles: results from a randomized, placebo-controlled trial in prostate cancer patients.

PURPOSE –: This study aims to investigate global metabolomic effects of soy protein supplementation. DESIGN/METHODOLOGY/APPROACH –: This st … total of 63 serum samples collected at baseline, 6 and 12months were analyzed by untargeted, global metabolomics. FINDINGS –: Partial least squares discriminant analysis, based on 493 polar metabolites and 4,706 lipids, showed clear separation of changes in metabolomic profiles between the soy and placebo groups. However, the only metabolite that was signific …

Serum Metabolomic Production in Parn-Stage Multical Sclerosis: Целевое сопоставление 1H-NMR, тип 1 и тип 2.

Анализ олигоклонной полосы (ОКС) в цереброспинной жидкости (CSF) остается краеугольным камнем ранней диагностики множественных склерозов (MS), при этом последние критерии указывают на то, что индекс беспозвоночной световой цепи (PP-FLC) является чувствительным маркером внутритехекулярного синтеза иммуноглобулина. Однако оба подхода основаны на поясничном проколе. Для удовлетворения потребностей в менее инвазивных инструментах в этом исследовании использовался протонный ядерный магнитный резонанс (1H-NMR) на основе метаболических метаболических сывороток для изучения вопроса о том, могут ли пер

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Oligoclonal band (OCB) analysis in cerebrospinal fluid (CSF) remains a cornerstone for the early diagnosis of multiple sclerosis (MS), with … for less invasive tools, this study employed proton nuclear magnetic resonance (1H-NMR)-based serum metabolomics to explore whether peripheral metabolic signatures can distinguish immunophenotypic subgroups, namely OCB Type 1 and Type 2, in patients with suspected early-stage MS. To investigate whether targeted proton nuclear magnetic resonance (1H-NMR) serum …

Analysis revealed the molecular mechanism of oxidative stress-autophagy-induced liver injury caused by high alkalinity: integrated whole hepatic transcriptome and metabolome.

INTRODUCTION: High-alkalinity water is a serious health hazard for fish and can cause oxidative stress and metabolic dysregulation in fish l … s and autophagy injury in the liver were analysed using whole-transcriptome miRNA-mRNA networks and metabolomics approaches. Our study provides new insights into liver injury caused by highly alkaline water.

Impact of Serotonin Transporter Absence on Brain Insulin Receptor Expression, Plasma Metabolome Changes, and ADHD-like Behavior in Mice fed a Western Diet.

The impaired function of the serotonin transporter (SERT) in humans has been linked to a higher risk of obesity and type 2 diabetes, especially as people age. Consuming a "Western diet" (WD), which is high in saturated fats, cholesterol, and sugars, can induce metabolic syndrome. Previous research indicated that mice carrying a targeted inactivation of the Sert gene (knockout, KO) and fed a WD display significant metabolic disturbances and behaviors reminiscent of ADHD. These abnormalities might be mediated via a dysfunction in insulin receptor (IR) signaling, which is also associated with adu

Branched-chain amino acid supplementation and voluntary running have distinct effects on the serum metabolome of rats with high or low intrinsic aerobic capacity.

INTRODUCTION: A growing body of literature associates branched-chain amino acid (BCAA) catabolism to increased fatty acid oxidation and better metabolic health. Hence, BCAA-rich diets may improve body composition and muscle protein synthesis. However, the role of individual characteristics such as a low aerobic fitness, a well-established risk factor for cardio-metabolic diseases, has not been studied. METHODS: This study examined 64 female rats from the high-capacity runner (HCR) and low-capacity runner (LCR) rat model. Rats from each line (HCR or LCR) were divided into four groups; differin

Widely targeted metabolomic and KEGG analyses of natural deep eutectic solvent-based saponins extraction from Camellia oleifera Abel.: Effects on composition.

Camellia saponins are important by-products of Camellia Oleifer Abel. processing. In this study, an eco-friendly method based on natural deep eutectic solvents (NaDESs, proline and glycerol at a molar ratio of 2:5) was established to extract saponins from C.oleifera cakes. The content of saponin (702.22 ± 1.28 mg/g) obtained using NaDES was higher than those extracted using water or methanol. UPLC-Q-TOF MS analysis of chemical structure showed that the difference in the extraction technique alter individual saponins. A widely targeted metabolomic approach and KEGG metabolic pathway analysis sh

Acute venous thromboembolism plasma and red blood cell metabolomic profiling reveals potential new early diagnostic biomarkers: observational clinical study.

BACKGROUND: Venous thromboembolism (VTE) is a leading cause of cardiovascular mortality. The diagnosis of acute VTE is based on complex imag … ificant differences were found between the two groups for clinical and demographic characteristics. Metabolomics assays identified and quantified a final number of 91 metabolites in plasma and 55 metabolites in red blood cells (RBCs). Plasma from acute VTE patients expressed tendency to a specific metabolomic signature, with univariate analyses revealing 23 si …

Inflammatory mediators, oxidative stress and genetic disturbance in rheumatoid arthritis rats supported by alfalfa seeds metabolomic constituents via blocking interleukin-1receptor.

Rheumatoid arthritis (RA) is an autoimmune disease characterized by aggressive cartilage and bone erosion. This work aimed to evaluate the metabolomic profile of Medicago sativa L. (MS) (alfalfa) seeds and explore its therapeutic impact against RA in rats. Arthritis was induced by complete Freund's adjuvant (CFA) and its severity was assessed by the arthritis index. Treatment with MS seeds butanol fraction and interlukin-1 receptor antagonist (IL-1RA) were evaluated through measuring interlukin-1 receptor (IL-1R) type 1 gene expression, interlukin-1 beta (IL-1β), oxidative stress markers, C-re

Urine organic acid metabolomic profiling by gas chromatography mass spectrometry: Assessment of solvent extract evaporation parameters on the recovery of key diagnostic metabolites.

BACKGROUND: Analysis of urinary organic acids (UOAs) by gas chromatography mass-spectrometry (GC-MS) is widely used in metabolomic studies. It is a complex test with many limitations and pitfalls yet there is limited evidence in the literature to support best practice. This study investigated the impact of drying down time and temperature on the recovery of 16 key analytes from solvent extracts. METHODS: Pooled urine specimens were enriched with organic acids. Urine aliquots (n = 3) were acidified and extracted into diethyl ether and ethyl acetate. Extracts were dried under nitrogen at ambien

Alteration of Gut Microbiota in Individuals at High-Risk for Rheumatoid Arthritis Associated With Disturbed Metabolome and the Initiation of Arthritis Through the Triggering of Mucosal Immunity Imbalance.

OBJECTIVE: In this study, we aimed to decipher the gut microbiome (GM) and serum metabolic characteristic of individuals at high risk for rheumatoid arthritis (RA) and to investigate the causative effect of GM on the mucosal immune system and its involvement in the pathogenesis of arthritis. METHODS: Fecal samples were collected from 38 healthy individuals and 53 high-risk RA individuals with anti-citrullinated protein antibody (ACPA) positivity (Pre-RA), 12 of 53 Pre-RA individuals developed RA within 5 years of follow-up. The differences in intestinal microbial composition between the healt