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In Vitro Assessment of Apoptotic and Cell Cycle Arrest Analysis on HepG2 Cells by Polyethylene Glycol-Coupled Selenium Nanocomposite Fabricated From Cassia fistula Flowers.

Polymer-encapsulated nanocomposite has been proven to have ameliorative effects in the treatment of cancer. The focused objective of the present research is to fabricate polyethylene glycol (PEG)-based Cassia fistula flower selenium nanocomposite (CFF-SeNC). The fabricated CFF-SeNC was characterised using a UV-visible spectrophotometer, Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM)-energy dispersive x-ray (EDX), and x-ray diffractometer (XRD) analysis. The highest UV-visible absorption spectra were at 355 nm. The oval-shaped, less agglomerated nanocomposite

Determination of Volatile Organic Compounds and Endogenous Extracts and Study of Expression Patterns of TPS and BSMT in the Flowers of Seven Lilium Cultivars.

Lily is one of the most important cut flowers in the world, with a rich floral fragrance. To further explore the fragrance emission mechanisms of lily cultivars, headspace solid-phase microextraction-gas chromatography-mass spectrometry (HS-SPME-GC-MS) and organic solvent extraction-gas chromatography-mass spectrometry (OSE-GC-MS) were used to unveil the volatile organic compounds (VOCs) and endogenous extracts of seven lily cultivars. Furthermore, real-time quantitative PCR (qRT-PCR) was used to determine the expression levels of two key genes (TPS and BSMT) related to the biosynthesis of mon…

A fast and simple FIA-chemiluminescence method for the evaluation of Roselle flowers as scavenger of the free radicals generated by UV irradiated antibiotics.

This work proposes a new method for the in vitro evaluation of the effect of UV irradiation on the production of free radicals and other reactive species during the photodecomposition of drugs. The method was based on the UV irradiation of antibiotics molecules to generate excited states that undergo to homolytic bond cleavages. These reactive species can be detected by their ability to oxidize the luminol, producing the electronically excited aminophtalate, which decays to the ground state releasing electromagnetic radiation in the visible zone of the spectrum. This method was applied to peni

Extremely high proportions of male flowers and geographic variation in floral ratios within male figs of Ficus tikoua despite pollinators displaying active pollen collection.

Most plants are pollinated passively, but active pollination has evolved among insects that depend on ovule fertilization for larval development. Anther-to-ovule ratios (A/O ratios, a coarse indicator of pollen-to-ovule ratios) are strong indicators of pollination mode in fig trees and are consistent within most species. However, unusually high values and high variation of A/O ratios (0.096-10.0) were detected among male plants from 41 natural populations of Ficus tikoua in China. Higher proportions of male (staminate) flowers were associated with a change in their distribution within the figs…

Molecular cloning of the cDNA coding for the (R)-(+)-mandelonitrile lyase of Prunus amygdalus: temporal and spatial expression patterns in flowers and mature seeds.

A gene highly expressed in the floral organs of almond (Prunus amygdalus Batsch), and coding for the cyanogenic enzyme (R)-(+)-mandelonitrile lyase (EC 4.1.2.10), has been identified and the full-length cDNA sequenced. The temporal expression pattern in maturing seeds and during floral development was analyzed by RNA blot, and the highest mRNA levels were detected in floral tissues. The spatial mRNA accumulation pattern in almond flower buds was also analyzed by in-situ hybridization. The mRNA levels were compared during seed maturation and floral development in fruit and floral samples from c

A Frosty Genetic Screen Unmasks a Major Regulatory Role for SHORT VEGETATIVE PHASE of Flowering in Response to a Cold Snap.

Flowering in plants is controlled by internal and environmental signals, with temperature playing a critical role. Accordingly, Arabidopsis thaliana plants show temperature-dependent variation in flowering time. Because unexpected cold periods can occur at any time, plants have evolved mechanisms to detect such cold snaps and delay flowering. Plants are more cold-tolerant in the vegetative stage, whereas flowers are sensitive and often show reduced reproductive success due to damage to floral structures and gametes. At the molecular level, delayed flowering can be caused by repression of the F…

Exogenous SNP alleviates high-temperature stress in rice at flowering via coordinated antioxidant, nitrogen, and hormone regulation.

High-temperature stress during flowering poses a severe threat to global rice production. Although nitric oxide (NO) is known to mediate plant stress responses, its protective role during this critical developmental stage remains poorly understood. In this study, we investigated the protective effects of the NO donor sodium nitroprusside (SNP) on a heat-tolerant cultivar (N22) and a heat-sensitive cultivar (YR343) exposed to a dynamic high-temperature regime (comprising five gradual transitions over a 24-h cycle within 30-38 °C). The results showed that foliar application of SNP at 150 μmol L-

Effects of In Vitro Static Digestion and Fermentation on Phenolic Profiles and Antioxidant Activity of Pitaya (Selenicereus undatus) Flower Tea.

Pitaya flower tea is a traditional hot-water infusion rich in polyphenols, but the digestive transformation, antioxidant activity, and intestinal transport potential of its water-extractable phenolics remain unclear. In this study, pitaya flower tea infusion was prepared by hot-water brewing to simulate ordinary consumption and then subjected to in vitro static oral, gastric, small-intestinal digestion, and simplified colonic enzymatic fermentation. Changes in total phenolic content, antioxidant activity, phenolic composition, and Caco-2 transport potential were evaluated using colorimetric as

Flower-rich and diverse road verges support pollinators, but traffic speed limits the ecological benefits across Europe.

Roads are vital for human societies, yet they can also have negative impacts on the ecological communities that live in close proximity to them. Insect pollinators, which nest and forage in road verges running alongside roads, are a group of particular importance. These verges may act as an "ecological trap," drawing insect pollinators into contact with traffic, increasing the risk of pollinator-traffic collisions. Spanning six European regions, we evaluated the complex relationships between traffic, road verge floral composition, and surrounding land use to understand how these factors influe

FvGI-FvCO-FvFT1 pathway activates FvTFL1 in long days to repress flowering in woodland strawberry (Fragaria vesca).

Photoperiod is a stable seasonal signal. Although photoperiodic flowering is well understood in short-day (SD) and long-day (LD) annual plants, the regulatory mechanisms in perennials remain elusive. In a perennial woodland strawberry (Fragaria vesca), flowering is induced in SDs in autumn and plants flower following spring, while in plants with mutated FvTERMINAL FLOWER1 (FvTFL1), LDs induce flowering. We investigated photoperiodic flowering of F. vesca through phenotypic and molecular characterization of transgenic lines and their crosses. We studied natural variation in flowering time and g

GWAS-driven discovery and KASP validation of flowering time and seed weight loci in lentil (Lens culinaris Medik.).

Lentil agronomic traits such as flowering time and seed weight are vital for adaptation and yield improvement. While prior quantitative trait loci (QTL) mapping and genome wide association studies (GWAS) have identified genomic regions associated with these traits, trait-linked KASP markers for routine breeding remain scarce in lentil. This study evaluated the agronomic traits of 200 lentil genotypes grown in Sanya over three years and combined with genotyping-by-sequencing (GBS) data. Analysis of variance revealed significant genotypic variation, and broad-sense heritability estimates were hi

Climate-Driven Shifts in Flowering Phenology of Wild Tropical Plants Uncovered by Century-Long Herbarium and Photographic Records.

Research on phenological responses to climate change has primarily focused on temperate regions, while tropical species remain critically understudied due to a historical scarcity of long-term monitoring data in these areas. We utilized digitized herbarium specimens and photographs to analyze century-long shifts (1921-2020) in the flowering phenology of 56 native plant species on Hainan Island, China. Species-specific linear models were used to quantify phenological responses of flowering day of year (DOY) to temperature and precipitation, and linear mixed-effects models were applied to test w

A Multifunctional Double-Array Petals Flower-Shaped Microfluidic Chip Combining Affinity and Physical Properties in Isolation of CTCs.

Circulating tumor cells (CTCs) are tumor cells that break away from the origin tumors and disseminate in the bloodstream and lymphatic circulation systems. CTCs originate from the original tumor with a similar bimolecular source. This makes CTCs play a vital status in cancer prognosis and diagnosis. However, CTC separation is highly challenging due to rarity and heterogeneity. In the present work, we designed a double-array petal flower-shaped microfluidic chip, a multifunctional capturing and isolation chip combining affinity and physical properties. The chip is composed of three arrays of mi

Antioxidant and selective cytotoxic activity of Dipterocarpus obtusifolius flower extract against gastrointestinal cancer, with bioactive component profiling.

BACKGROUND: Gastrointestinal (GI) malignancies exhibit significant global variations in incidence and mortality. Late diagnosis remains a ma … ion using real-time RT-PCR. Phytochemical profiling was also conducted via GC-MS and NMR. RESULTS: Flowers extraction of Dipterocarpus obtusifolius Teijsm. ex Miq. showed stronger FRAP activity was seen in the ethyl acetate extract, corresponding to its higher phenolic content, while greater DPPH scavenging activity (EC50 = 0.8681 mg/mL) and higher flavo …

Effectiveness of FTD/TPI plus bevacizumab and impact of prior bevacizumab exposure in patients with mCRC: the Italian FLOWER study.

BACKGROUND: The SUNLIGHT trial established trifluridine/tipiracil (FTD/TPI) plus bevacizumab as a new later-line standard in metastatic colorectal cancer (mCRC). However, evidence in real-world populations-often older and more heterogeneous-remains limited. This study evaluated the effectiveness and safety of FTD/TPI plus bevacizumab in a large, unselected real-world mCRC population and aimed to identify potential predictive factors associated with response to this treatment. PATIENTS AND METHODS: We conducted a retrospective, multicenter analysis of patients with refractory mCRC treated with

Molecular Mechanisms Underlying the Alleviation of Aβ-Induced Toxicity by Edible Flower Buds of Hemerocallis citrina in Caenorhabditis elegans.

Dried daylily flower buds, rich in polyphenols, are an edible vegetable renowned for their nutritional and health benefits. This study employed Caenorhabditis elegans (C. elegans) as a model organism to investigate the protective effects of the ethyl acetate fraction from the ethanol extract of Hemerocallis citrina (HC-EA) against amyloid-beta (Aβ) toxicity and elucidate its underlying mechanisms The results demonstrated that HC-EA significantly alleviated Aβ-induced paralysis and extended lifespan in C. elegans, while exhibiting no observable toxicity at concentrations ranging from 0.1 to 1 m

Anti-hyperplastic effects of Acmella oleracea flower and leaf extracts in prostate cell lines and in spontaneously hypertensive rats.

Benign prostatic hyperplasia (BPH) is a highly prevalent age-associated disorder and a leading cause of lower urinary tract symptoms in men worldwide. Given the limitations of current therapies, there is increasing interest in phytotherapeutic compounds as sources of biologically active agents. Acmella oleracea, a medicinal plant rich in the alkamide spilanthol, has been traditionally associated with urogenital effects; however, the biological impact of distinct plant organs on prostate hyperplasia remains poorly defined. In this study, we investigated the effects of flower (A.Fl) and leaf (A.

Biocompatible 3D hierarchical flower-like iron-doped silver nanostructures as a platform for in vitro and in vivo drug delivery.

Nanostructured platforms have attracted significant attention as promising drug carriers due to their unique properties and therapeutic efficiency. This work aims to prepare three-dimensional flower-like iron-doped silver nanostructures (HFAg-Fe) and evaluate their potential as nanocarriers for Methotrexate (MTX), a first-generation anticancer and autoimmune drug. The HFAg-Fe were synthesized, modified, and characterized by FE-SEM, FT-IR, XRD, AFM, and elemental analyses. Drug loading and release were studied using UV–vis spectroscopy, showing an MTX loading capacity of 86% and pH-responsive b

Computational model of flower pattern evolution predicts spontaneous emergence of boundary cell types across the petal epidermis.

Petal patterns play an important role in the reproductive success of flowering plants by attracting pollinators and protecting against environmental factors. Some transcription factors (TFs) involved in petal epidermal cell differentiation have been identified, but little is known about the upstream processes that pre-pattern the petal surface to establish their expression domains. Here, we have developed a computational model of petal pattern evolution to investigate this pre-patterning phase, selecting for gene regulatory network (GRNs) that divide the petal into proximal and distal domains

Разоблачение неврологических выгод: обзор гемплического листа, цветка, экстракции семенного масла и их фитохимических свойств в неврологических расстройствах.

Неврологические расстройства, такие, как эпилепсия, болезнь Альцгеймера, болезнь Паркинсона и множественный склероз, представляют собой значительные глобальные проблемы в области здравоохранения, связанные со сложной патофизиологией и ограниченными терапевтическими возможностями, которые часто оказывают существенное побочное воздействие. Приобретенные гемпами соединения, в частности семена сативы Каннабиса, листья и цветы, привлекли внимание к их потенциальным нейрозащитным свойствам. Цель этого обзора заключается в обобщении текущих данных о терапевтических выгодах, связанных с гемповыми соед

Совпадение в аннотации оригинала
Neurological disorders such as epilepsy, Alzheimer's disease, Parkinson's disease, and multiple sclerosis present significant global health care challenges, with complex pathophysiology and limited therapeutic options that often carry substantial side effects. Hemp-derived compounds, particularly from Cannabis sativa seeds, leaves, and flowers, have gained a…