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Site of gene action of the white allele (Miwh) of the microphthalmia locus: a dermal-epidermal recombination study.

The white allele Miwh of the microphthalmia locus, when homozygous, causes a complete absence of neural crest-derived melanocytes in skin and internal organs. The site(s) of gene action of Miwh on melanoblast differentiation were examined by making dermal-epidermal recombinant grafts with skin from 13-14-day embryos of the following genotypes: normal (+/+), heterozygous white (Miwh/+), homozygous white (Miwh/Miwh), and dominant spotting (W/W). Based on results from these grafts, the following conclusions were reached: Miwh/Miwh epidermis is as effective as W/W epidermis in supporting the diffe

[Classification of leukemias].

The principles according to modern views and practical needs of classification of acute leukemia are discussed on the basis of morphological properties of the leukemic cells after panoptic staining and of their cytochemical reactions. This form of classification gives some indications for the choice of treatment and for the prognosis. It is also the permission for the evaluation of therapeutic results, as are the other factors relevant for the prognosis.

Antiviral efficacy of Trappin-2/Elafin against Zika virus in the human keratinocyte HaCaT cell line.

Zika virus (ZIKV) is primarily transmitted through mosquito bites, and the skin acts as the initial site of viral entry into the host. Conse … ral activity of Tr2/E in human keratinocytes during ZIKV infection. In this study, we evaluated the permissiveness of the human keratinocyte cell line HaCaT to infection with a Mexican isolate of ZIKV and observed that these cells support productive viral infection. We then assessed whether ZIKV infection induces endogenous expression of Tr2/E. Tr2/E transcripts …

From Growth Trajectory to Functional Decline: Age-Contextualized Nutritional Strategies for Muscle Vulnerability. A Narrative Review.

Muscle vulnerability occurs at both extremes of the human lifespan, although its biological significance differs substantially between devel … l bioenergetics. In pediatric contexts, nutritional interventions primarily aim to restore anabolic permissiveness within a structurally intact growth environment. In aging individuals, strategies focus on mitigating anabolic resistance through optimized protein intake, correction of micronutrient insufficiencies, and integration with resistance exercise to pres …

SARS-CoV-2 Establishes a Productive Infection in Hepatoma and Glioblastoma Multiforme Cell Lines.

Severe acute respiratory syndrome associated coronavirus 2 (SARS-CoV-2) emerged at the end of 2019 and rapidly caused a pandemic that led to … imary astrocytes or neuroblastoma cells did not support replication of the virus. Glioblastoma cell permissiveness is associated with defects in interferon production. Overall, these results suggest that liver dysfunction during COVID-19 is not due to infection of these tissues by SARS-CoV-2. Furthermore, tumors may potentially serve as reservoirs for the virus …

Rift Valley fever virus modulates apoptosis and immune response during infection of human astrocytes.

Rift Valley fever (RVF) is an arboviral disease of zoonotic origin that causes recurrent epidemics in Africa, the Arabic Peninsula, and isla … f the CNS that have several supporting roles including immune response regulation. We confirmed the permissiveness of astrocytes to RVFV infection and highlighted a strain-dependent infectivity. We showed that RVFV infection of astrocytes induced cell apoptosis and observed that the RVFV Non-Structural protein NSs, a known virulence factor, potentially delayed a …

Methamphetamine Enhances Cryptococcus neoformans Melanization, Antifungal Resistance, and Pathogenesis in a Murine Model of Drug Administration and Systemic Infection.

Methamphetamine (METH) is a major public health and safety problem in the United States. Chronic METH abuse is associated with a 2-fold-high … . Our findings provide novel evidence of the impact of METH abuse on host homeostasis and increased permissiveness to opportunistic microorganisms.

FHL1 is a major host factor for chikungunya virus infection.

Chikungunya virus (CHIKV) is a re-emerging alphavirus that is transmitted to humans by mosquito bites and causes musculoskeletal and joint pain1,2. Despite intensive investigations, the human cellular factors that are critical for CHIKV infection remain unknown, hampering the understanding of viral pathogenesis and the development of anti-CHIKV therapies. Here we identified the four-and-a-half LIM domain protein 1 (FHL1)3 as a host factor that is required for CHIKV permissiveness and pathogenesis in humans and mice. Ablation of FHL1 expression results in the inhibition of infection by several …

TREX1 531C>T Polymorphism is Associated with High Proviral Load Levels in HTLV-1-Infected Persons.

Human T-lymphotropic virus type 1 (HTLV-1) deregulates the immune system and cell cycle, resulting in loss of immune tolerance and disease, including HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP). Three prime repair exonuclease 1 (TREX1) maintains innate immune tolerance of the host and host-cell permissiveness to retroviral infections. TREX1 polymorphisms may influence the course of infection and autoimmune manifestations. The influence of TREX1 531C/T polymorphism was investigated in HTLV-1 infection and development of symptoms among 151 persons infected with HTLV-1 (32…

Project STYLE: a multisite RCT for HIV prevention among youths in mental health treatment.

OBJECTIVE: The study examined the efficacy of family-based and adolescent-only HIV prevention programs in decreasing HIV risk and improving … improvements in parent-teen sexual communication (p<.01), parental monitoring (p<.01), and parental permissiveness (p=.05). CONCLUSIONS: This RCT found that the HIV prevention interventions reduced sexual risk behavior over three months in a large, diverse sample of youths in mental health treatment and that the family-based intervention improved parental monit …

Low self-control and co-occurrence of gambling with substance use and delinquency among Chinese adolescents.

Relatively little is known about gambling co-morbidity in Asian youth populations. The role of trait self-control in co-morbidity also remai … significantly (p < .001) predicts at-risk/probable pathological gambling, frequent gambling, strong permissiveness toward gambling, heavy use of tobacco and alcohol, and delinquent involvement, even after controlling for the potential shared correlates of socioeconomic characteristics, parental monitoring and peer delinquency. Hence, the concept that gambling pr …

Первичные эндотелиальные клетки человека поддерживают прямое, но не зависающее от антител усиление вирусной инфекции денге.

Микроваскулярная утечка плазмы является отличительной чертой геморрагической лихорадки денге и шокового синдрома денге. Точные молекулярные механизмы, ведущие к утечке микроваскула, пока еще не определены, однако в качестве основной причины этого заболевания была предложена инфекция вируса денге (ДЕНВ) и последующая смерть эндотелиальных клеток. Однако масштабы эндотелиальной разрешимости клеточной инфекции DENV и масштабы смерти клеток после инфекции DENV являются противоречивыми. Чтобы прояснить этот вопрос, мы проанализировали кинетику и последствия заражения DENV человека пумельными эндоте

Совпадение в аннотации оригинала
Microvascular plasma leakage is the hallmark of dengue hemorrhagic fever and dengue shock syndrome. The precise molecular mechanisms leading to microvascular leakage are yet to be determined, but dengue virus (DENV) infection and consequent endothelial cell death has been suggested as its major cause. However, the extent of endothelial cell permissiveness to…

Purification of Schwann cells by selection of p75 low affinity nerve growth factor receptor expressing cells from adult peripheral nerve.

The intrinsic capacity of Schwann cells to promote regeneration after limited peripheral nerve lesions has been successfully transferred to extensive peripheral nerve injuries and central nervous system lesions by autologous transplantation strategies. However, both the intrinsic ability of axotomized neurons to regenerate and the permissiveness of the parenchyma surrounding the acute injury site diminish over time. Therefore, the autologous transplantation mode requires a fast and effective method to isolate Schwann cells from peripheral nerve biopsies. Here, we report a method to purify p75 …

Australian perspective on Fourth Consensus Guidelines for the management of postoperative nausea and vomiting.

This is a summary document that provides an Australian perspective on the Fourth Consensus Guidelines for the management of postoperative nausea and vomiting. The Australian Society of Anaesthetists has endorsed the Fourth Consensus Guidelines for the management of postoperative nausea and vomiting and has written this document with permission from the authors and the American Society for Enhanced Recovery to provide an Australia-specific summary.

[Cerebral and physical risks associated with cannabis use].

This article summarize the results of research studies that indicate that the accepting, permissive approach to cannabis use is not justified. After theoretical introduction and a discussion of the forms currently available, we review research and epidemiological surveys demoting the effects of cannabis on various body systems. The deleterious effects associated with cannabis use and its derivatives are discussed.

A sex police for adults with "mental retardation"? Comment on Spiecker and Steutel.

This article is a rebuttal of the claim by Spiecker and Steutel that sex between people with mild and moderate "mental retardation" is morally permissable only with the substitutive consent of caregivers. After a review of historical, empirical and practical considerations, an ethical analysis is undertaken which concludes that Spiecker and Steutel's arguments are deeply flawed and their proposed policy morally objectionable.

In vitro and in vivo cytotoxic activity of native and ricin conjugated monoclonal antibodies to HBs antigen for Alexander primary liver cell carcinoma cells and tumours.

In in vitro and in vivo systems, native or ricin conjugated monoclonal anti-HBs, are capable of inhibiting or slowing the growth of Alexander primary hepatocellular carcinoma cells. Failure of the immune response to this component of the hepatitis B virus may be one permissive factor in the development of some primary liver cell carcinoma in chronic HBV carriers.

Experimental Infection of Peromyscus Species Rodents with Sin Nombre Virus.

We demonstrate that 6 distinct Peromyscus rodent species are permissive to experimental infection with Sin Nombre orthohantavirus (SNV). Viral RNA and SNV antibodies were detected in members of all 6 species. P. leucopus mice demonstrated markedly higher viral and antibody titers than P. maniculatus mice, the established primary hosts for SNV.

[Carcinogenic properties of nitrosodimethylamine when combined with benzene, cadmium, boron or ethanol].

A combination of benzene (5.0 mg/l) or cadmium (0.01 mg/l) with nitrosodimethylamine (drinking water, 9 months) produced a significantly higher tumor incidence in female CBA X C57B1/6 mouse hybrids. The said agents are capable of modifying the carcinogenicity of other substances when present in water in concentrations exceeding the permissible one.

Haemophilus influenzae in the airways: canary in the coal mine or driver of disease?

INTRODUCTION: Overgrowth and colonization by non-typeable Haemophilus influenzae (NTHi) is a common feature of increasing disease severity, treatment resistance and increased susceptibility to disease exacerbations across chronic airways diseases (CADs). Whether NTHi is a driver of respiratory disease or reflects that the damaged airway has become a permissive environment for growth remains to be proven. AREAS COVERED: In this review, we discuss the potential roles of hypermutation, biofilm formation and intracellular living in allowing NTHi to adapt to living in the lungs of individuals with