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[Genome labeling of the streptomyces strain HS007].

By construction of small fragments genome bank, several different fragments from chromosomal DNA of streptomyces production strain HS007 were cloned into cloning vector pSP-SIM. According to the sequencing results, five of them, which were 3- to 7- kb in size with single restriction sites in the middle, were cut down and re-cloned into bacteria-streptomyces shuttle vector pHJL400. Subsequently, the marker cassette, composed of special labeling sequence, oriT, 2 FLP recognition target sites and apramycin resistance gene, was inserted into the single restriction sites to create recombinant plasm

The impact of processing meat and fish products on phosphorus intake in chronic kidney disease patients.

INTRODUCTION AND OBJECTIVES: The use of phosphate additives in meat and fish processing leads to a phosphorus overload that we cannot quantify through labelling or food composition tables. We analysed this increase by measuring phosphorus content in these products by spectrophotometry. MATERIALS AND METHOD: We determined the phosphorus/protein ratio in fresh meat and fish products with varying degrees of processing by spectrophotometry (phosphorus) and the Kjeldahl method (proteins). We contrasted these results with those reflected in the food composition tables. RESULTS: The phosphorus/prot

Prevalence, Reach of Anti-smokeless Tobacco Messages and Quit Attempts by Product Type: A Secondary Data Analysis from the Global Adult Tobacco Survey (GATS - 2016-17).

OBJECTIVE: India has the highest number of smokeless tobacco (SLT) products available in different forms, consumed in various ways. The current study aimed to understand the pattern of daily SLT use according to different product categories and whether Quit intention and Quit attempts vary by SLT type and exposure to media messages. METHODS: Data from Global Adult Tobacco Surveys (GATS), 2016-17, was used to find access to media messages and warning labels by SLT type. Quit attempt and Quit intention were calculated for each of the SLT types. Logistic regression analyses were employed to iden

A review of patient-reported outcomes used for regulatory approval of oncology medicinal products in the European Union between 2017 and 2020.

IntroductionCancer and corresponding available treatments are associated with substantial symptoms and functional limitations. In this context, collection of patient-reported outcomes (PRO) in clinical trials gained special interest and is recommended by regulatory authorities. Within clinical trials framework, PRO may provide evidence to support medicines approval, labeling and marketing claims. This study aims to analyze the existing evidence based on PRO as part of new oncology indications receiving positive opinions issued by the European Medicines Agency (EMA) between 2017 and 2020 and to

Совпадение в полном тексте источника
… Patient-reported outcomes in support of oncology product labeling claims: regulatory context and challenges. Expert Rev Pharmacoeconom Outcomes Res. (2010) 10:407–20. 10.1586/erp.10.45 16. LeBlanc TW, Abernethy AP. …

A survey of formaldehyde in shampoos and skin creams on the Danish market.

To evaluate the exposure of the general population to formaldehyde from the use of cosmetic products, as well as to monitor whether cosmetic products comply with national regulations, 285 shampoos, creams, etc., were analysed for formaldehyde. Identification and determination of formaldehyde was performed by the EEC method for the analysis of formaldehyde in cosmetic products. It was shown that 29.5% of the products investigated contained 0.001%-0.147% total formaldehyde. In 10 of the products (3.5%), total formaldehyde content was > 0.05%. 8 of these products contained > 0.05% free formaldehy

Development of an ultra-high performance liquid chromatography-tandem mass spectrometry method for the determination of anabolic steroids currently available on the black market in the Czech Republic and Slovakia.

Over the past decade, the use of anabolic androgenic steroids (AAS) by recreational bodybuilders and regular gym members has been increasing exponentially. However, there is a lack of knowledge on AAS products sold in the Czech Republic and Slovakia. The aim of this study was to assess the quantity of active substances in AAS products obtained from AAS users in the Czech Republic and Slovakia. In addition, the study also examines the current trends in counterfeit AAS products used by recreational bodybuilders and regular gym members. For this purpose, the authors developed and validated a univ

[Determination of carbohydrate content in soft drinks. Methodological aspects and results of research using various methods].

An important component of the programs to prevent type 2 diabetes mellitus, obesity, and other chronic noncommunicable diseases is limiting the intake of added sugars. Information about the composition of the product and its nutritional value contained in the labeling contributes to the informed choice of consumers when forming a diet. On July 1, 2023, new provisions of the Tax Code of the Russian Federation came into force, providing for the imposition of an excise tax on "sugar-containing drinks" - soft drinks made with added sugar and containing carbohydrates in quantities of more than 5 g/

Detection of DNA from undeclared animal species in commercial elimination diets for dogs using PCR.

BACKGROUND: Elimination diets are the gold standard for the diagnosis of adverse food reactions (AFR). A broad variety of commercial diets are available containing either hydrolysed protein or novel ingredients which claim to be suitable for elimination diets. Contamination may be one factor accounting for the failure of commercial elimination diet trials. HYPOTHESIS/OBJECTIVES: To test commercial diets labelled as suitable for elimination diets for dogs, for DNA of animal origin other than that declared on the label. METHODS: Twelve commercial dry and tinned dog food products were investiga

A polymerase chain reaction-enzyme immunoassay for diagnosis of pneumococcal meningitis in children and adults.

BACKGROUND AND OBJECTIVES: meningitis due to Streptococcus pneumoniae is common among children and adults. In this study a polymerase chain reaction (PCR) for diagnosis of pneumococcal meningitis was evaluated prospectively. METHODS: a total of 61 cerebrospinal fluid specimens were included prospectively using defined inclusion and exclusion criteria. These samples were studied by PCR-EIA and results compared with conventional microbiological procedures and antigen detection techniques. Primers were used against the conserved region of the pneumococcal autolysin gene and the amplified product

Fermentation and subsequent disposition of 14C-labelled plant cell wall material in the rat.

A 14C-labelled plant cell wall preparation (14C-PCW) produced from spinach (Spinacia oleracea L.) cell culture exhibits uniform labelling of the major polysaccharide groups (%): pectins 53, hemicellulose 13, cellulose 21, starch 3. This 14C-PCW preparation has been used in rat studies as a marker for plant cell wall metabolism. Metabolism of the 14C-PCW occurred largely over the first 24 h. This was due to fermentation in the caecum. The pectic fraction of the plant cell walls was degraded completely in the rat gastrointestinal tract, but some [14C]cellulose was still detected after 24 h in th

The origin, kinetics and distribution of large lymphocytes from the thoracic duct of rats with trichinosis.

Studies were done on the distribution and recirculation of thoracic duct lymphocytes from rats infected with Trichinella spiralis larvae. Previously it had been found that in rats with trichinosis, when large lymphocytes were transferred into normal rats, eosinophil leucocyte production was increased. These lymphocytes, which constituted up to 30 per cent of the cells in thoracic duct lymph, were specifically labelled with tritiated thymidine and they were followed in tissues and lymph. As some of the labelled large lymphocytes were found in bone marrow, it was postulated that eosinophil produ

Oxidant generation by single infected monocytes after short-term fluorescence labeling of a protozoan parasite.

Leishmania spp. are intracellular protozoa residing in mononuclear phagocytes. Leishmania organisms are susceptible to microbicidal responses generated in response to phagocytosis. Assuming that both phagocyte and parasite populations are heterogeneous, it is advantageous to examine the response of individual cells phagocytosing living parasites. Because Leishmania spp. lose virulence during the raising of transfectants, we developed a method to label live Leishmania chagasi short-term with fluorescent dyes. Up to six parasite divisions were detected by flow cytometry after labeling with carbo

High sensitivity of chemiluminescent methodology for detection of clonal CDR3 sequences in patients with acute lymphoblastic leukemia.

Detection of minimal residual disease (MRD) in patients with B-cell acute lymphoblastic leukemia (B-ALL) has been achieved using several radioactive labelling methodologies; however, limited information exists about the use of chemiluminescent labelling. Although many malignant disorders are related to cytogenetic alterations, there is not a consistent chromosomal translocation that could serve as a tumour marker for the monitoring of MRD. ALL are derived from B-lymphocytes in 80% of cases. In the early stages of their maturation, the immunoglobulin heavy chain genes (IgH) undergo rearrangemen

Allergenic characterization of best-selling personal lubricants.

Personal lubricants vary appreciably in ingredient composition. Although some products are labeled hypoallergenic, these claims are seldom evidence-based. The objective was to determine the potential allergenicity of popular lubricants and elucidate the validity of hypoallergenic product claims. This cross-sectional study involved publicly available data on Amazon's 50 best-selling lubricants as of July 2023. Product ingredient lists were cross-referenced with the North American Contact Dermatitis Group and American Contact Dermatitis Society allergen series. Associations were explored between

Prostacyclin production in regions of arterial stenosis.

The effect of abnormal flow dynamics on prostacyclin (PGI2) production by intact endothelium is unknown. To investigate this we studied the effects of graded stenoses on vessel wall PGI2 production in dogs (n = 8) whose femoral and carotid arteries (n = 32) were narrowed by machine-milled clips, producing 1.0 cm segmental stenoses of 25%, 50%, 75%, and 90% diameter reduction. Three dogs were injected with Indium 111-labeled platelets and 12 vessels were scanned for platelet deposition. All stenotic vessels were excised at 6 weeks for histologic study (hematoxylin-eosin section and immunohistoc

Renal gammagraphy with 99mTc-phosphomycin.

99mTc-Phosphomycin, a new radiopharmaceutical for kidney visualization, was used for animal experiments and tests of 171 patients. The results confirmed the usefulness of this product. The ease and yield of the labelling procedure, the low cost of the product, the excellent quality of the images and the functional information obtained showed that the use of 99mTc-phosphomycin as a radiopharmaceutical for kidney visualization has many advantages.

Isolation, Identification, and Validation of Strains from Commercial Probiotics: Do We Get What We Expect?

This study evaluated the viability, microbiological composition, functional traits, and safety of probiotic bacteria isolated from commercial products marketed as containing Limosilactobacillus reuteri. Viable cell counts, biochemical characterization, strain-level identification, functional properties, gastrointestinal tolerance, and safety attributes were assessed. Among the evaluated products, only four presented colony-forming units (CFU) counts consistent with label claims (products E, F, G, and H), while two showed no detectable viable microorganisms (products B and L). All isolates were

A simple and affordable kinetic assay of nucleic acids with SYBR Gold gel staining.

Labeling substrates or products are paramount in determining enzymatic kinetic parameters. Several options are available; many laboratories use either radioactive or fluorescent labeling because of their high sensitivity. However, those methods have their own drawbacks such as half-life decay, expensive and hazardous. Here, we propose a novel, simple, economical and fast alternative to substrate labeling for studying the kinetics of nucleic acids: post-migration gel staining with SYBR Gold. Cleavage rates similar to the ones reported in the literature for the I-R3 DNA-cleaving DNA enzyme in th

Fluorine-18 labeling of small molecules: the use of 18F-labeled aryl fluorides derived from no-carrier-added [18F]fluoride as labeling precursors.

The favourable long-half life, the ease of production and the low energy of the emitted positron make 18F an ideal radionuclide for PET imaging. Radiochemistry of 18F basically relies on two distinctive types of reactions: nucleophilic and electrophilic reactions. All syntheses of 18F-labeled radiotracers are based on either [18F]fluoride ion or [18F]fluorine gas as simple primary labeling precursors which are obtained directly from the cyclotron. They can be applied either directly to the radiosynthesis or they can be transformed into more complex labeling precursors enabling the multi-step b

Degradation of 2-sec-butyl-4,6-dinitrophenol (dinoseb) by Clostridium bifermentans KMR-1.

A strain of Clostridium bifermentans, KMR-1, degraded 2-sec-butyl-4,6-dinitrophenol (dinoseb) to a level below the limit of detection by high-performance liquid chromatography (0.5 mg/liter) within 96 h, with no accumulation of aromatic intermediates. KMR-1 could not utilize dinoseb as a sole carbon or energy source, and degradation occurred via cometabolism in the presence of a fermentable carbon source. KMR-1 mineralized some dinoseb in anaerobic cultures, evolving 7.2% of the radioactive label in U-ring 14C-labeled dinoseb as 14CO2. The remaining anaerobic degradation products were incubate