Результаты поиска

Performance Evaluation of a Dengue IgG Rapid Diagnostic Test Designed to Determine Dengue Serostatus as Part of Prevaccination Screening.

The World Health Organization has recommended prevaccination screening for prior dengue infection as the preferred approach prior to vaccination with the dengue vaccine CYD-TDV. These screening tests need to be highly specific and sensitive, and deliverable at the point-of-care. We evaluate here the sensitivity and specificity of the newly developed OnSite Dengue IgG rapid diagnostic test (RDT). A retrospective double-blind study of the sensitivity and specificity of the OnSite Dengue IgG RDT was performed using a sample panel consisting of archived serum specimens collected during CYD-TDV cli…

Performance of a New Microfluidic Dengue NS1 Immuno-magnetic Agglutination Assay for the Rapid Diagnosis of Dengue Infection in Adults.

Dengue (DENV) infections are a public health concern worldwide and thus early diagnosis is important to ensure appropriate clinical management. The rapid diagnostic test (RDT) targets nonstructural protein 1 (NS1) detection and is the main tool used for diagnostic purpose. In this study, we evaluated the performance of a new rapid and semi-quantitative microfluidic DENV NS1 immuno-magnetic agglutination assay or IMA (ViroTrack Dengue Acute, BluSense Diagnostics, Copenhagen, Denmark). We studied 233 subjects confirmed to have DENV infection (by a real-time reverse transcriptase polymerase chain…

Non-structural protein 1 (NS1) of dengue virus detection correlates with severity in primary but not in secondary dengue infection.

BACKGROUND: Non-structural protein 1 (NS1) of dengue virus circulates in the serum of patients during the acute phase of the disease. OBJECTIVES: To determine whether NS1 screening can serve in diagnosing primary and secondary infection and to evaluate its utility as a marker for predicting the severity of dengue in children. STUDY DESIGN: Patients ≤15 years of age hospitalized for dengue between 2012-2018, with NS1 determination (Panbio, Australia) were included. Clinical y laboratorial characteristics were collected in a standardized data table for analysis of correlation between serotypes…

Detection of post-vaccination enhanced dengue virus infection in macaques: An improved model for early assessment of dengue vaccines.

The need for improved dengue vaccines remains since the only licensed vaccine, Dengvaxia, shows variable efficacy depending on the infecting dengue virus (DENV) type, and increases the risk of hospitalization for severe dengue in children not exposed to DENV before vaccination. Here, we developed a tetravalent dengue purified and inactivated vaccine (DPIV) candidate and characterized, in rhesus macaques, its immunogenicity and efficacy to control DENV infection by analyzing, after challenge, both viral replication and changes in biological markers associated with dengue in humans. Although DPI…

Emergence of genotype Cosmopolitan of dengue virus type 2 and genotype III of dengue virus type 3 in Thailand.

Dengue is a mosquito-borne disease that has spread to over 100 countries. Dengue fever is caused by dengue virus (DENV), which belongs to the Flavivirus genus of the family Flaviviridae. DENV comprises 4 serotypes (DENV-1 to DENV-4), and each serotype is divided into distinct genotypes. Thailand is an endemic area where all 4 serotypes of DENV co-circulate. To understand the current genotype distribution of DENVs in Thailand, we enrolled 100 cases of fever with dengue-like symptoms at the Bamrasnaradura Infectious Diseases Institute during 2016-2017. Among them, 37 cases were shown to be dengu…

Indices of anti-dengue immunoglobulin G subclasses in adult Mexican patients with febrile and hemorrhagic dengue in the acute phase.

Heterologous secondary infections are at increased risk of developing dengue hemorrhagic fever (DHF) because of antibody-dependent enhancement (ADE). IgG subclasses can fix and activate complement and bind to Fcɣ receptors. These factors may also play an important role in the development of ADE and thus in the pathogenesis of DHF. The aim of this study was to analyze the indices of anti-dengue IgG subclasses in adult patients with febrile and hemorrhagic dengue in the acute phase. In 2013, 129 patients with dengue fever (DF) and 57 with DHF in Veracruz, Mexico were recruited for this study and…

Validation of probability equation and decision tree in predicting subsequent dengue hemorrhagic fever in adult dengue inpatients in Singapore.

We developed a probability equation and a decision tree from 1,973 predominantly dengue serotype 1 hospitalized adult dengue patients in 2004 to predict progression to dengue hemorrhagic fever (DHF), applied in our clinic since March 2007. The parameters predicting DHF were clinical bleeding, high serum urea, low serum protein, and low lymphocyte proportion. This study validated these in a predominantly dengue serotype 2 cohort in 2007. The 1,017 adult dengue patients admitted to Tan Tock Seng Hospital, Singapore had a median age of 35 years. Of 933 patients without DHF on admission, 131 progr…

The impact of a program for strengthening dengue hemorrhagic fever case management on the clinical outcome of dengue hemorrhagic fever patients.

This study compared the case fatality ratio (CFR) of dengue shock syndrome (DSS) patients admitted to Buri Ram Hospital, an area with CFR of 0.11, 0.43 and 0.23% in 2002, 2003 and 2004, respectively, to obtain a provincial model for dengue case management using the I. development of a special program for strengthening dengue hemorrhagic fever (DHF) case management (No deaths in DSS patients), II. a retrospective review of the medical records of dengue fever (DF), DHF and DSS patients referred to Buri Ram Hospital. We compared the data during the 3 periods of the implementation of this program.…

Использование неструктурного белка 1 антигена денге для ранней диагностики в фабричной стадии у пациентов с инфекцией денге.

ОЦЕНКА ИСПОЛЬЗОВАНИЯ НЕСТРУКТУРНОГО белка 1 (NS1) для раннего диагностирования в фабричной стадии у пациентов с инфекцией денге. МЕТОДЫ: В общей сложности 445 сер, полученных от 165 пациентов [диапазонной лихорадки (DF): 42, геморрагическая лихорадка денге (DHF) первого класса: 50, II: 63, III и IV: 10] и 8 других женских заболеваний в возрасте от 5 до 15 лет, были расценены на антигены NS1, специфические иг М и Иг G.Результаты: Положительные показатели по антигену NS1 среди пациентов с либо DF, либо DHF составили 100% (7 из 7) в день 2, 92,3% (12 из 13) в день 3, 76,9% (40 из 52) в день 4, 56

Совпадение в аннотации оригинала
BACKGROUND: To evaluate the use of dengue nonstructural protein 1 (NS1) antigen for the early diagnosis during the febrile stage in patients with dengue infection. METHODS: A total of 445 sera obtained from 165 patients [dengue fever (DF): 42, dengue hemorrhagic fever (DHF) grade I: 50, II: 63, III and IV: 10] and 8 other febrile illnesses 5-15 years of age…

Co-circulations of two genotypes of dengue virus in 2006 out-break of dengue hemorrhagic fever in Karachi, Pakistan.

BACKGROUND: The status of dengue genotypes involved in the recent epidemic out-breaks in Pakistan is not well defined. OBJECTIVES: We sought to analyze the predominant genotype responsible for the most severe and largest out-break of dengue hemorrhagic fever (DHF) that hit Karachi in 2006. STUDY DESIGN: Retrospective analysis of stored serum samples for dengue virus genotype by multiplex RT-PCR, anti-dengue IgM, IgG and review of clinical charts of patients admitted to Aga Khan University Hospital. RESULTS: Viral RNA detection of 250 patients revealed positive results in 185 (74.0%) samples…

Evaluating the sensitivity of a commercial dengue NS1 antigen-capture ELISA for early diagnosis of acute dengue virus infection.

INTRODUCTION: The aim of this report is to establish an accurate diagnosis of acute dengue virus infection early, in order to provide timely information for the management of patients and early public health control of dengue outbreak. METHODS: 224 serum samples from patients with a clinical diagnosis of acute dengue infection, which were subsequently confirmed by laboratory tests, were used to evaluate the performance of a commercially-available dengue NS1 antigen-capture ELISA kit. RESULTS: The dengue NS1 antigen-capture ELISA gave an overall sensitivity rate of 93.3 percent (209/224). The…

Immune Evasion Mechanisms of Neurotropic Dengue Virus.

Dengue virus (DENV) is an important human pathogen that infects millions of people each year and can cause diseases such as dengue haemorrhagic fever and dengue shock syndrome. Despite the efforts of the immune system to control the innate and adaptive immune responses to the virus, DENV escapes from immune responses using complex and multiple mechanisms. In certain cases, these immune evasion strategies may also contribute to the neurotropic potential of DENV, facilitating its ability to invade or affect CNS. This narrative review summarises the current knowledge by focusing on major immune e…

Dengue Suppression by Male Wolbachia-Infected Mosquitoes.

BACKGROUND: Wild-type female Aedes aegypti mosquitoes that mate with male A. aegypti mosquitoes that have been infected with the wAlbB strain of Wolbachia pipientis bacteria produce nonviable offspring owing to cytoplasmic incompatibility. Repeated releases of wolbachia-infected males can potentially suppress wild-type mosquito populations and reduce the risk of dengue virus infection. METHODS: We conducted a trial involving the release of male A. aegypti mosquitoes infected with the wAlbB strain of wolbachia bacteria for the control of dengue in Singapore, a tropical city-state. In this clus…

Mixed fungal infection in immunocompetent Dengue patient.

Invasive fungal infection (IFI) simultaneously due to three fungi is rare. IFI caused by two fungal agents is relatively commoner in transplant patients. Most common IFI worldwide is candidiasis, whereas in India, aspergillosis and mucormycosis infections are commoner. Severe dengue viral infection has been linked with invasive fungal infections. Following case report exemplifies more than two fungi can together cause pneumonia as confirmed by similar findings in 3 respiratory samples from the dengue shock syndrome patient. High clinical suspicion and prompt initiation of aggressive, high-dose…

The Ultimate Buzzkill: When Dengue Hits Hard.

Dengue cases have been rising globally in recent years. In the United Arab Emirates (UAE) specifically, weather conditions have created an ideal breeding ground for the Aedes aegypti mosquito, the primary vector of the disease. We report the case of a 62-year-old South Asian male residing in the UAE who initially experienced vague flu-like symptoms. His condition progressively worsened, ultimately resulting in an unexpected out-of-hospital cardiac arrest and, tragically, death.

Reverse genetics rescue of sylvatic dengue viruses.

UNLABELLED: There are an unknown number of sylvatic ("sylvan" = "of the forest") dengue viruses currently being sustained by nonhuman primates and mosquitoes in the forests of Africa and Asia. Humans are regularly infected with these viruses, occasionally resulting in small epidemics. One barrier to understanding sylvatic dengue virus biology is the scarcity of stocks available for study. While the full-length genome sequences of 28 sylvatic viruses exist on GenBank, accessible infectious stocks exist for only a handful of these. One way to overcome this obstacle is to rescue infectious virus …

How dengue defies dogma: An extrafollicular tale.

The acute humoral immune response in patients with severe dengue can be extrafollicular, driven by a unique subset of CD4+ T cells.

Unraveling Psoriasis Flare-up and Dengue Infection.

IMPORTANCE: Dengue infection is an emerging global health concern. Understanding its impact on patients with a history of psoriasis is essential, particularly concerning its potential to trigger psoriasis flare-ups. Additionally, it is crucial to assess the influence of psoriasis treatments on the severity of dengue. OBJECTIVES: This study aims to describe the effects of dengue infection on psoriasis exacerbations, in patients with pre-existing psoriasis and to assess whether prior psoriasis treatment influences dengue severity. DESIGN: A cross-sectional descriptive study. MAIN OUTCOMES AND…