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Serological studies on the etiological role of measles-like virus in subacute sclerosing panencephalitis.

Significantly higher CSF titers of hemagglutination (HI) measles virus antibody were found in 22 patients with subacute sclerosing panencephalitis (SSPE) and more elevated titers of measles virus neutralization (NV) antibody were encountered in 19 patients with SSPE than in 50 children and young adults with other neurological diseases. Similar differences occurred in the sera of patients and controls. The serum: CSF antibody ratio was examined in 15 SSPE cases. All but one patient showed reduced antibody ratio (less than 80) as compared with the normal index. In addition, serological studies i

Complement-dependent cytotoxicity of sera obtained from subacute sclerosing panencephalitis patients.

Human lymphoid cells (NC-37) persistently infected with either measles virus (Schwarz and TYCSA strains) or subacute sclerosing panencephali … cell lines, indicating that the viral surface antigens of these cell lines infected with measles or SSPE virus are identical. On the other hand, the cytotoxic activity of SSPE sera could not be readily absorbed with these cells. Thus, the affinity of SSPE sera for the viral surface antigens might be lower than that of measles convalescent sera.

Demonstration of a blocking factor in the plasma and spinal fluid of patients with subacute sclerosing panencephalitis. I. Partial characterization.

Conflicting reports on the immune responsiveness of patients with subacute sclerosing panencephalitis (SSPE) have been reported. This report shows that the leucocytes from four SSPE patients exhibited strong sensitivity to both measles and SSPE virus preparations as measured by the macrophage migration inhibition test, mixed lymphocyte virus infected cell culture test, and the lymphotoxin assay. Earlier suggestions that a factor may be operating to suppress cellular reactivity are confirmed by the demonstration that the response of lymphocytes from SSPE patients could be blocked by the additio…

[Subacute sclerosing panencephalitis (SSPE)].

Subacute sclerosing panencephalitis (SSPE) is a progressive and fatal central nervous system disorder that results from a persistent SSPE virus infection. The efficacy of inosine pranobex, an antiviral/immunomodulator agent, remains controversial. Intraventricular interferon-alpha combined with oral inosine pranobex slows the progressive course of SSPE, but does not cure the disease. We examined a wide variety of antiviral compounds for their inhibitory effects on SSPE virus strains in vitro and in vivo, and found that ribavirin had potent inhibitory activity against SSPE. We tried to treat SS…

Evidence that the hypermutated M protein of a subacute sclerosing panencephalitis measles virus actively contributes to the chronic progressive CNS disease.

Subacute sclerosing panencephalitis (SSPE) is a progressive degenerative disease of the brain uniformly leading to death. Although caused by measles virus (MV), the virus recovered from patients with SSPE differs from wild-type MV; biologically SSPE virus is defective and its genome displays a variety of mutations among which biased replacements of many uridine by cytidine resides primarily in the matrix (M) gene. To address the question of whether the SSPE MVs with M mutations are passive in that they are not infectious, cannot spread within the CNS, and basically represent an end-stage resul…

Antiviral Effect of Favipiravir (T-705) against Measles and Subacute Sclerosing Panencephalitis Viruses.

Subacute sclerosing panencephalitis (SSPE) is a late-onset, intractable, and fatal viral disease caused by persistent infection of the central nervous system with a measles virus mutant (SSPE virus). In Japan, interferon-α and ribavirin are administered intracerebroventricularly to patients with SSPE. However, as the therapeutic effect is insufficient, more effective drugs are needed. Favipiravir, which is clinically used as an anti-influenza drug, demonstrates anti-viral effects against RNA viruses. In this study, the antiviral effect of favipiravir against measles virus (Edmonston strain) an…

Inhibition of measles virus and subacute sclerosing panencephalitis virus by RNA interference.

Subacute sclerosing panencephalitis (SSPE) is a rare, but fatal outcome of measles virus (MeV) infection. SSPE develops after prolonged persistence of mutated MeV called SSPE virus. Although a combination therapy using interferon and inosiplex or ribavirin appears to prolong survival time to some extent, there is currently no effective treatment to completely cure SSPE and a new treatment strategy is greatly needed. In this study, we adopted RNA interference (RNAi) strategy and examined whether small interfering RNAs (siRNAs) can be used to inhibit replication of MeV and SSPE virus. We report …

Characterization of virus-specific RNAs from subacute sclerosing panencephalitis virus-infected CV-1 cells.

CV-1 cells infected with subacute sclerosing panencephalitis (SSPE) virus incorporated uridine-(3)H into at least four virus-specific RNA co … NA annealing experiments using unlabeled 50s SSPE RNA with labeled 18s RNA from cells infected with SSPE virus or measles virus show 100% annealing with 18s SSPE RNA but only 60% annealing with 18s measles RNA. These experiments suggest some differences between the 18s RNAs of SSPE virus-infected cells and measles virus-infected cells.

Molecular analysis of structural protein genes of the Yamagata-1 strain of defective subacute sclerosing panencephalitis virus. IV. Nucleotide sequence of the fusion gene.

The full-length cDNA corresponding to the mRNA of the fusion (F) protein of the Yamagata-1 strain of subacute sclerosing panencephalitis (SS … cular weight of 57,963. The homology of the amino acid sequence of the F protein between the MV and SSPE virus was 96.27%, and the positions of cysteine and proline were almost identical in the two viruses. The functional domains of SSPE-virus F protein closely resembled those of MV F protein, including the cleavage site, a signal sequence, the fusion-relate …

Characterization of measles virus-specific proteins synthesized in vivo and in vitro from acutely and persistently infected cells.

Measles virus protein synthesis has been analyzed in acutely and persistently infected cells. To assess the role of measles in subacute sclerosing panencephalitis (SSPE), measles viral proteins synthesized in vivo or in vitro were tested for reactivity with serum from a guinea pig(s) immunized with measles virus and sera from patients with SSPE. Guinea pig antimeasles virus serum immunoprecipitates the viral polypeptides of 78,000 molecular weight (glycosylated [G]), 70,000 molecular weight (phosphorylated [P]), 60,000 molecular weight (nucleocapsid [N]), and 35,000 molecular weight (matrix [M

A Novel Peptide Derived from the Fusion Protein Heptad Repeat Inhibits Replication of Subacute Sclerosing Panencephalitis Virus In Vitro and In Vivo.

Subacute sclerosing panencephalitis (SSPE) is a persistent, progressive, and fatal degenerative disease resulting from persistent measles virus (MV) infection of the central nervous system. Most drugs used to treat SSPE have been reported to have limited effects. Therefore, novel therapeutic strategies are urgently required. The SSPE virus, a variant MV strain, differs virologically from wild-type MV strain. One characteristic of the SSPE virus is its defective production of cell-free virus, which leaves cell-to-cell infection as the major mechanism of viral dissemination. The fusion protein p…

Observations of subacute sclerosing panencephalitis in South Africa.

Analysis of 75 cases of subacute sclerosing panencephalitis (SSPE) reported to the National Institute for Virology, South Africa, in 1984-1990 does not support the role of intensive exposure to measles virus in the pathogenesis of SSPE. The incidence of SSPE per million population was similar in Blacks and Whites, although that of reported measles is up to 10 times greater in Blacks. The age of SSPE follows the distribution of measles cases; thus, significantly more younger SSPE cases were found in Blacks than in Whites. The distribution between males and females was approximately equal. These

Detection of measles virus antigen(s) in peripheral lymphocytes from patients with subacute sclerosing panencephalitis.

Peripheral blood lymphocytes from 5 patients with subacute sclerosing panencephalitis (SSPE) were stimulated with phytohemagglutinin (PHA) and analyzed for the presence of the measles virus antigen(s) by immunofluorescence (IF). For detection of viral antigen fluorescein-conjugated globulins from SSPE patients or from measles convalescents both with high anti-measles titer were used. Peripheral blood lymphocytes from 5 children with measles were used as a positive control. Measles virus antigen(s) were localized in PHA-transformed lymphocytes in all SSPE patients. The present results indicate

Гемедсорбция, выраженная клонированными генами Н из вирусов подводного склероза панэнцефалита (SSPE) и их возможных вирусов кори потомства, изолированных в Осаке, Япония.

Большинство вирусов склероза панэнцефалита (ССПЕ), включая наш осака-1, -2 и -3 штаммы, изолированные в Осаке, показали отрицательное кровопоглощение (HAD) африканскими зелеными красными кровяными клетками. Считается, что это вещество характерно для вируса SSPE по сравнению с позитивной реакцией стандартного Эдмонстонского штамма вируса кори (MV). Однако это предположение стало довольно неясным, потому что MV мутирует часто на генетическом уровне во время его умножения, а также потому, что недавние полевые штаммы, изолированные лимфобаластоидными линиями клеток, продемонстрировали отрицательны

Совпадение в аннотации оригинала
Most subacute sclerosing panencephalitis (SSPE) viruses, including our Osaka-1, -2, and -3 strains isolated in Osaka, have shown negative hemadsorption (HAD) by African green monkey red blood cells. This property has been thought to be characteristic of SSPE virus as compared to the positive reaction of the standard Edmonston strain of measles virus (MV). Ho…

Субакустическое склерозирование панэнцефалита - пакистанская перспектива.

В районах мира, где широко распространен вирус кори, сохраняется проблема склерозирования панэнцефалита (ССПЕ) из-за отсутствия приверженности всеобщей вакцинации. Недавно мы изучили 89 случаев, совместимых с клиническим диагнозом SSPE и тестированных на антитела в сыворотке или цереброспинной жидкости для вируса кори. Одиннадцать случаев были подтверждены в качестве SSE на основе хорошо подкрепляемых лабораторных данных.

A serum protein affecting the regulation of the immune system.

A thermostable alpha 2 globulin inhibiting the immunoglobulin/anti-immunoglobulin reaction was demonstrated working with subacute sclerosing … coated cups. This was demonstrated, working on the one hand with measles virus strain Edmonston or SSPE virus strain D.R. and SSPE serum and on the other hand with IgG from SSPE and control serum. This alpha 2 globulin, an inhibiting protein, appears to be related to "normal immunosuppressive protein".

Retinitis as the presenting feature of subacute sclerosing panencephalitis in an Indian male: A case report.

Measles virus is a rare but important cause for acute retinitis as it can eventually lead to the fulminant complication of SSPE. We report a case of a young Indian male with acute viral retinitis who subsequently developed SSPE. It is of paramount importance to consider measles virus and SSPE as a cause in an immunocompetent young adult with necrotizing viral retinitis.

[Eye manifestations of subacute sclerosing panencephalitis].

A report is given on 10 children with subacute sclerosing panencephalitis (SSPE) whose clinical illness began with visual or mental impairme … . A positive antibody titer for toxoplasmosis, which can occur parallel with the typical measles or SSPE virus antibody titer, occasionally causes the misdiagnosis of toxoplasmosis acquisita. In the 10 cases reported on, the diagnosis SSPE was confirmed on the basis of the typical clinical features and elevated measles virus antibody titer. In no case the pe …

[Slow virus infection].

Subacute sclerosing panencephalitis (SSPE) and progressive multifocal leukoencephalopathy (PML) are the major slow virus infections of humans. SSPE is caused by mutant measles virus after long persistence in the brain. Its pathogenesis is discussed, based on accumulating data on molecular properties on measles virus prevalent in the field as well as recent identification of measles virus receptor. Current situation on the therapy of SSPE is also described. PML is caused by the infection of oligodendroglia with persisting JC virus. Its pathogenesis is discussed based on the molecular properties

Humoral and cellular immune responses to matrix protein of measles virus in subacute sclerosing panencephalitis.

The immune response to matrix (M) protein of measles virus was examined in patients with subacute sclerosing panencephalitis (SSPE) and controls. Antibodies specific for M and nucleocapsid (NC) proteins in 11 serum and 8 cerebrospinal fluid (CSF) samples from patients with SSPE were quantitated by enzyme-linked immunosorbent assay by using affinity-purified measles virus proteins. Geometric mean anti-NC antibody titers were higher in the serum (6.58 +/- 0.98 [mean +/- standard deviation]) and CSF (4.38 +/- 0.74) of SSPE patients compared with controls. Anti-M antibodies were present in the ser