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On the Mechanism of Cytoprotection by Ferrostatin-1 and Liproxstatin-1 and the Role of Lipid Peroxidation in Ferroptotic Cell Death.

Ferroptosis is a form of regulated necrosis associated with the iron-dependent accumulation of lipid hydroperoxides that may play a key role in the pathogenesis of degenerative diseases in which lipid peroxidation has been implicated. High-throughput screening efforts have identified ferrostatin-1 (Fer-1) and liproxstatin-1 (Lip-1) as potent inhibitors of ferroptosis - an activity that has been ascribed to their ability to slow the accumulation of lipid hydroperoxides. Herein we demonstrate that this activity likely derives from their reactivity as radical-trapping antioxidants (RTAs) rather t…

[Effect of tobacco smoke on lipids peroxidation and liver function in streptozotocin diabetic rats--preliminary study].

Diabetes is considered a group of diseases with chronic hyperglycemia caused by various organ disorders, failure or damage as a common featu … D) in many tissues. The aim of this study was to evaluate influence of exposure to tobacco smoke on lipid peroxidation and liver function in experimentally induced diabetes. The research showed that the protein level in blood serum did not change neither in case of induced diabetes nor after tobacco smoke exposure. However a statistically significant increase of lip …

[Changes in the processes of the lipid peroxidation and antioxidant defense system in patients with obstructive sleep apnoea syndrome].

The objective of this study was to evaluate the lipid peroxidation (LPO) and antioxidant defense (AoD) system in patients with varying hypoxia in obstructive sleep apnoea/hypnoea syndrome (OSA/HS). The study of the LPO-AOD system in patients with OSA/HS revealed the specific features of some metabolic reactions that could reflect the development of adaptive-compensatory processes. The patients with upper airway hyperresistance syndrome were found to have increases in general blood antioxidant activity (AOA) and dienic conjugate levels, which might be interpreted as an initial stress reaction o…

[Effects of TCE and PCE on cultured human keratinocyte lipid peroxidation and protective effect of vitamin E on it].

OBJECTIVE: To explore the different concentrations of trichloroethylene (TCE) or perchloroethylene (PCE) induced cultured normal human epidermal keratinocyte (KC) lipid peroxidation and protective effect of Vitamin E on it. METHODS: KC derived from 3 or more donors were pooled together and cultured with K-SFM. Neutral Red Uptake Assay was used to determine the IC50 of TCE or PCE, and then different concentrations of TCE or PCE were administered for culturing KC; 0.5 mmol/L TCE or 0.2 mmol/L PCE and different concentrations of Vitamin E were used to determine the protective effect of Vitamin E…

Lipid peroxidation is enhanced in patients with systemic lupus erythematosus and is associated with arterial and renal disease manifestations.

OBJECTIVE: Cardiovascular disease with premature atherosclerosis is common in patients with systemic lupus erythematosus (SLE). We previously identified elevated levels of oxidized low-density lipoprotein (OxLDL) together with elevated levels of autoantibodies related to OxLDL as risk factors for cardiovascular disease in female patients with SLE. Autoantibodies to OxLDL are common in SLE and cross-react with anticardiolipin antibodies (aCL). We therefore hypothesized that lipid peroxidation is enhanced in patients with SLE in general. METHODS: One hundred forty-seven female patients with SLE…

Hepatic glutathione and lipid peroxidation in rats treated with theophylline. Effect of dose and combination with caffeine and acetaminophen.

As theophylline, caffeine and acetaminophen (APAP) are commonly found in combination in prescription and non-prescription drugs, the present study was designed to evaluate changes of hepatic glutathione (GSH) and lipid peroxidation in rats treated concurrently with these widely used drugs. In rats treated with different doses of theophylline, a dose-related depletion of hepatic GSH was observed (r = -0.88, P < 0.001). After a dose of 100 mg/kg of caffeine given alone and in combination with 50 mg/kg of theophylline, hepatic GSH levels were decreased by 22.5% (P > 0.05) and 35.5% (P < 0.01) of …

[The role of hyperoxidation in changes of lipid peroxidation and tissue metabolism in patients undergoing surgery with artificial circulation].

53 patients with various heart diseases subject to cardiopulmonary bypass surgery have been examined. To assess the state of tissue metabolism indexes of arterial and mixed venous blood oxygen balance, lactate concentration, the activity of energy-dependent lymphocyte enzymes and indexes of free-radical plasma processes have been studied. It has been shown that cardiopulmonary bypass in conditions of marked normobaric hyperoxidation (PaO2 more than 300 mm Hg) is accompanied by tissue metabolism intensification and disturbance. Inability of protective antioxidant mechanisms to react to the inte

Plasma lipid peroxides in murine sepsis--sex differences and effect of antioxidative/anti-inflammatory therapy.

In order to investigate the influence of antioxidative/anti-inflammatory combination therapy (AACT) with dimethyl sulfoxide (DMSO), chlorpromazine (CPZ) and vitamin E upon the activity of the inflammation, plasma lipid peroxide was measured as thiobarbituric acid reactive substance (TBARS) 12 hrs postoperatively in the modified cecal ligation sepsis model in the mouse. Significantly higher TBARS levels were found in the male control group (13.7 +/- 0.7 nmol MDA/ml) than in the female control group (11.6 +/- 0.6 nmol MDA/ml). The operated male group had significantly higher TBARS levels (16.2 +

TFPT Abundance Is Associated with Growth-Related Phenotypes and Cellular Features Linked to Lipid Peroxidation Defense in Hepatocellular Carcinoma.

TCF3 fusion partner (TFPT) remains insufficiently characterized in hepatocellular carcinoma (HCC), particularly in relation to tumor growth and cellular features linked to lipid peroxidation defense. We integrated public transcriptomic and clinical datasets and established stable TFPT-knockdown and TFPT-overexpression models in MHCC97-H and Huh-7 cells. TFPT expression was higher in HCC tissues and selected HCC cell lines. In TCGA-LIHC, higher TFPT expression was associated with shorter overall survival after multivariable adjustment (HR, 1.37; 95% CI, 1.06-1.77), although this association was…

Industrial additive and dietary fiber inulin induces tumor-selective necrotic death via mitochondrial lipid peroxidation involving MYC and YAP.

Inulin, as a prebiotic fiber and an industrial additive, has been shown to regulate gut and small intestinal microbiomes for health, but non … indicating organelle stress coupled to metabolic reprogramming. Non-malignant cells showed elevated lipid peroxidation but limited necrotic death, consistent with greater metabolic tolerance. YAP/MYC inhibition increased inulin sensitivity. Importantly, ataxia telangiectasia and Rad3-related (ATR) supported stress adaptation, particularly in normal cells. Additional …

Whole-body mass spectrometry imaging reveals metabolome and lipid peroxidation heterogeneity in zebrafish xenografts of esophageal squamous cell carcinoma.

Esophageal squamous cell carcinoma (ESCC) is a highly prevalent malignancy in China. Understanding its metabolic heterogeneity is of great s … ssues, while long-chain FAPPs predominated in poorly differentiated tumors, suggestive of disparate lipid peroxidation patterns in ESCC cells at different differentiation stages. Furthermore, distinct signature metabolites were found to be specifically accumulated in the brain providing experimental clues that ESCC may remotely disturb the central nervous system met …

Длиннокаменистые толстые кислоты как движущие факторы нейроинфламмации в нейродегенерации: механистические связи с липидом пероксидацией, Ферроптозом и митохондриальной дисфункцией.

Фон: Нейродегенеративные заболевания (НИ) рассматриваются главным образом как расстройства, характеризующиеся серьезным иммунометаболическим дисбалансом, характеризующимся текущей нейроинфламмацией и глиальной активацией. Хотя митохондриальная дисфункция и окислительный стресс являются хорошо известными особенностями, метаболические факторы в верхнем течении, связывающие эти патологические процессы, по-прежнему плохо понимаются. Методы: В ходе этого обзора мы изучили недавние доклинические и клинические исследования, в ходе которых были изучены связи между липидным метаболизмом, иммунометаболи

Совпадение в аннотации оригинала
Background: Neurodegenerative diseases (NDs) are mainly considered disorders marked by severe immunometabolic imbalance, characterized by on … e. Notably, high levels of specific LCFAs, especially arachidonic acid, increase ROS production and lipid peroxidation. This lipotoxic environment ultimately triggers ferroptosis, an iron-dependent form of cell death shared across multiple NDs. Conclusions: The harmful interaction among mitochondrial dysfunction, lipid peroxidation, and ferroptosis is driven by an i …

Maresin-1 alleviates lipid peroxidation-induced ferroptosis after radiation-induced brain injury in mice through the RORα/NRF2 pathway.

Ferroptosis plays a critical role in radiation-induced brain injury (RIBI). The role of Maresin-1, which has anti-inflammatory and antiferro … est. Changes in the number of NeuN-positive neurons were detected through immunohistochemistry. The lipid peroxidation level was evaluated by detecting the contents of malondialdehyde (MDA), 4-hydroxynonenal (4-HNE), glutathione-reduced (GSH) and glutathione-oxidized (GSSG). The expression of the ferroptosis-related markers glutathione peroxidase 4 (GPX4) and cycloo …

SLC27A2 marks lipid peroxidation in nasal epithelial cells driven by type 2 inflammation in chronic rhinosinusitis with nasal polyps.

Chronic rhinosinusitis with nasal polyps (CRSwNP) is characterized by persistent inflammation and epithelial cell dysfunction, but the underlying molecular mechanisms remain poorly understood. Here we show that dysregulated lipid metabolism and increased lipid peroxidation in nasal polyp epithelial cells contribute to the pathogenesis of CRSwNP. Integrated analysis of bulk and single-cell RNA sequencing data reveals upregulation of SLC27A2/FATP2 in nasal polyp epithelium, which correlates with increased lipid peroxidation. SLC27A2-positive epithelial cells exhibit enriched expression of lipid …

Кислотный профиль эритроцитов по множеству склерозов связан с курсами болезней, липидной пероксидацией и диетическим влиянием.

Предыстория/цели: Диетический образ жизни, в частности потребление жирных кислот (FA), может быть полезным для смягчения основных патогенных процессов при множественном склерозе (MS); однако данные по-прежнему являются недостаточными, особенно в том, что касается передачи болезни. Таким образом, цели этого исследования заключались в изучении эритроцитного профиля АУ у пациентов с релапсирующими (RR)MS и прогрессивными (P)MS, а также в изучении вопроса о том, является ли пищевая добавка пищевой добавкой n-3 PUFA может повлиять на профиль FA, в зависимости от хода болезни. Методы: профиль FA был

Совпадение в аннотации оригинала
Background/Objectives: Dietary lifestyle, particularly the intake of fatty acids (FAs), may be useful in alleviating the key pathogenic proc … , and a positive one between MSSS and 22:6n-3 (DHA). The observed decrease in levels of circulating lipid peroxidation product 4-HNE in PMS patients was not found in the n-3 PUFA supplementation group. Conclusions: The present findings suggest that the changes in the levels of FAs and their correlations are specific for the course of MS. Detected FA profile differen …

Transferrin-mediated increase of labile iron Pool following simulated ischemia causes lipid peroxidation during the early phase of reperfusion.

Heart ischemia/reperfusion (I/R) injury is related to iron content. However, the occurrence and mechanism of changes in labile iron pool (LI … changes correlated with blebbing of cell membrane and SR-induced LDH release. These data suggested lipid peroxidation occurred via Fenton's reaction. The experiments using bafilomycin A1 and zinc protoporphyrin suggested no role of ferritinophagy or heme oxidation in the increase of LIP during SI. The extracellular source, transferrin assessed using serum transferr …

Lipid peroxidation and liver damage in double and simple common bile duct ligation models in male Sprague-Dawley rats.

AIMS: Bacterial translocation, defined as the presence of living bacteria or bacterial fragments in both mesenteric lymph nodes or systemic circulation, can cause a severe inflammatory reaction in patients with cirrhosis. This study aimed to compare lipid peroxidation associated with liver damage in different experimental models of bile duct ligation: proximal double ligation and transection versus proximal simple ligation versus sham. MATERIALS AND METHODS: Sixty-two male rats underwent one of three bile duct surgical interventions: proximal double ligation and transection (n = 22); proximal…

Cysteine hydropersulfide reduces lipid peroxidation and protects against myocardial ischaemia-reperfusion injury - Are endogenous persulfides mediators of ischaemic preconditioning?

Earlier studies revealed the presence of cysteine persulfide (CysSSH) and related polysulfide species in various mammalian tissues. CysSSH h … at the onset of reperfusion rescues functional impairment and myocardial damage by interfering with lipid peroxidation. Utilising a well-established ex vivo Langendorff murine model, we here demonstrate that endogenous tissue concentrations of CysSSH are upregulated when oxygen supply is compromised (global myocardial ischaemia) and rapidly restored to baseline leve …

Effect of Tungstate Administration on the Lipid Peroxidation and Antioxidant Parameters in Salivary Glands of STZ-Induced Diabetic Rats.

Sodium tungstate is an alternative to reduce hyperglycemia for the treatment of diabetes. In previous work, we showed that the administration of sodium tungstate increased the specific activity of salivary amylase in the parotid gland. Here, we investigated the effect of the administration of sodium tungstate on the lipid peroxidation and some antioxidant parameters in the submandibular (SM) and parotid (PA) salivary glands of streptozotocin (STZ)-induced diabetic rats. Thirty-two male Wistar rats were divided into four groups (n = 8, each): control (C), control treated with sodium tungstate (…