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Apoptosis-inducing protein, AIP, from parasite-infected fish induces apoptosis in mammalian cells by two different molecular mechanisms.

AIP (apoptosis-inducing protein) is a protein purified and cloned from Chub mackerel infected with the larval nematode, Anisakis simplex, which induces apoptosis in various mammalian cells including human tumor cell lines. AIP has shown structural and functional homology to L-amino acid oxidase (LAO) which oxidizes several L-amino acids including L-lysine and AIP-induced apoptosis has been suggested to be mediated by H2O2 generated by LAO activity of AIP. In this study, we confirmed that recombinant AIP generated enough H2O2 in culture medium to induce rapid apoptosis in cells and this apoptos

Genetic and morphological approaches distinguish the three sibling species of the Anisakis simplex species complex, with a species designation as Anisakis berlandi n. sp. for A. simplex sp. C (Nematoda: Anisakidae).

Numerous specimens of the 3 sibling species of the Anisakis simplex species complex (A. pegreffii, A. simplex (senso stricto)), and A. simplex sp. C) recovered from cetacean species stranded within the known geographical ranges of these nematodes were studied morphologically and genetically. The genetic characterization was performed on diagnostic allozymes and sequences analysis of nuclear (internal transcribed spacer [ITS] of ribosomal [r]DNA) and mitochondrial (mitochondrial [mt]DNA cox2 and rrnS) genes. These markers showed (1) the occurrence of sympatry of the 2 sibling species A. pegreff

A recombinant enolase from Anisakis simplex is differentially recognized in natural human and mouse experimental infections.

A 1,963-bp cDNA was isolated from an Anisakis simplex cDNA library by immunoscreening with a hyperimmune rabbit serum raised against a crude extract of A. simplex L3 larvae. The open reading frame encodes a putative protein of 436 amino acid residues, which exhibits high similarity (70-80%) to enolase molecules from various other organisms, including helminth parasites. After subcloning and expression of the A. simplex cDNA in PGEX-4T-3, the resulting glutathione S-transferase fusion protein, purified by glutathione-Sepharose-4B chromatography, showed functional enolase activity. The immunogen

Haematological Diseases in the Tropics

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Совпадение в полном тексте источника
… Pulmonary hydatid disease Paragonimiasis Coccidioidomycosis and paracoccidioidomycosis Non-parasitic causes of eosinophilia and respiratory symptoms Gastrointestinal symptoms | Strongyloidiasis Schistosoma mansoni and S. japonicum Ascariasis Tapeworm Dwarf tapeworm Hookworm Whipworm Pin worm Trichinellosis Anisakiasis …

Резюме ЭСП 2013.

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Резюме

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Совпадение в полном тексте источника
… The number of eosinophils in biopsies can be very high and even suggestive of primary eosinophilic enteritis, e.g. in trichuriasis, anisakiasis and enterobiasis, and with Ancylostoma caninum and Angiostrongylus infections. … Alternatively, chronic inflammation with eosinophilia may be confused with ulcerative colitis or Crohn’s disease, the latter especially if granulomas are present (as with Schistosoma mansoni infection and more rarely in strongyloidiasis, anisakiasis and enterobiasis). …

Abstracts from the 37th Annual Meeting of the Society of General Internal Medicine, 2014, San Diego, CA, USA.

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