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Decellularized ovarian bioscaffolds and resveratrol-loaded polymeric nanoparticles support in vitro viability and ultrastructural preservation of bovine preantral follicles.

PURPOSE: This study evaluated the effects of decellularized ovarian bioscaffolds and resveratrol-loaded polymeric nanoparticles (RLPN) on th … or unencapsulated resveratrol. Follicular viability and ultrastructure were evaluated by calcein-AM/ethidium homodimer-1 staining and transmission electron microscopy. Expression of mRNA for catalase, superoxide dismutase, glutathione peroxidase 1, peroxiredoxin 6, and nuclear factor erythroid 2-related factor 2 was assessed by qRT-PCR. Quantitative data w …

Cytosolic DNA-induced CGAS-STING1-dependent autophagy promotes SFTSV and EV-71 replication.

The CGAS-STING1 pathway is an innate immune system that can detect double-stranded DNA in the cytoplasm and trigger antiviral immune respons … eticulum; ERGIC: endoplasmic reticulum-Golgi intermediate compartment; EV-71: enterovirus 71; EtBr: ethidium bromide; Gn: glycoproteins N; IFN-I: type I interferon; KO: knockout; MOIs: multiplicities of infection; NP: nucleoprotein; NSs: non-structural proteins; ROS: reactive oxygen species; SFTSV: Severe fever with thrombocytopenia syndrome virus; STING1: …

Phytochemical characterization of methanolic extract of Cuminum cyminum seeds and its anticancer efficacy against human oral cancer.

BACKGROUND: Oral squamous cell carcinoma (OSCC) accounts for over 90% of oral and pharyngeal malignancies and remains a leading cause of can … od against Staphylococcus aureus and Escherichia coli. Apoptosis was assessed using acridine orange/ethidium bromide (AO/EtBr) and Hoechst 33342 staining. RESULTS: HPLC analysis revealed multiple phytochemical constituents, with a major peak at 9.90 min. MECC exhibited dose-dependent cytotoxicity, with predicted IC₅₀ values of approximately 600 μg/mL (KB) …

In vitro and in silico antiviral activity of Polyscias scutellaria leaf extract against drug-susceptible and drug-resistant herpes simplex virus and human papillomavirus-positive cervical cancer: implications for topical formulation development.

Herpes simplex virus (HSV) and human papillomavirus (HPV) infections remain major global health concerns, particularly in immunocompromised … and HeLa cell growth, using plaque inhibition, HPV-16 pseudovirus binding, wound-healing, acridine/ethidium dual staining, and flow cytometry assays. Gene and protein expression levels were analyzed by quantitative qPCR and western blot analysis. The ethanolic extract of fresh P. scutellaria exhibited the strongest inhibitory effects against both wild-typ …

Extraction solvent shapes the phytochemistry, multi-target bioactivity, and machine learning-predicted anticancer targets of Seseli libanotis.

Seseli libanotis (L.) W. D. J. Koch (Apiaceae) is a wild vegetable traditionally consumed in eastern Anatolia, but its non-volatile phytoche … 50 31.77-41.25 µg mL-1). Annexin V and propidium iodide staining (Annexin V/PI) and acridine orange/ethidium bromide (AO/EB) staining indicated apoptosis-associated viability loss; however, similar activity against HEK-293 cells indicated limited tumor selectivity. Extraction solvent was the principal determinant of phytochemical composition and bioactivit …

TXA11114: Discovery of an In Vivo Efficacious Efflux Pump Inhibitor (EPI) That Potentiates Levofloxacin Against Pseudomonas aeruginosa.

Objectives: Multidrug-resistant (MDR) Pseudomonas aeruginosa represents a major clinical challenge, driven in part by resistance-nodulation- … against panels of clinical isolates. Mechanistic studies included levofloxacin accumulation assays, ethidium bromide accumulation assays, outer-membrane permeability measurements, and whole-genome sequencing of mutants with altered potentiation phenotypes. In vivo efficacy was evaluated in murine thigh and lung infection models, while preliminary safety an …

A promising therapeutic biomaterial: CHX@ZnO-HA/PSSS nanocomposites enable pH-Dependent infection eradication and bone regeneration in osteomyelitis.

OBJECTIVES: Osteomyelitis is a considerable clinical problem, necessitating multifunctional biomaterials that can concurrently manage infect … using MG-63 osteoblast-like cells, which were assessed via the MTT assay, dual acridine orange and ethidium bromide staining, scratch assays, and real-time polymerase chain reaction analysis of osteogenic markers (BMP2, RUNX2, osteocalcin, alkaline phosphatase, and type 1 collagen). RESULTS: Characterization tests demonstrated the effective incorporation …

Dual-Peptide Nanoplatform: Mesoporous Silica Nanoparticles Functionalized With a Cell-Penetrating Peptide and Loaded With Rationally Designed Antimicrobial Peptides for Tuberculosis Therapy.

Tuberculosis remains a major infectious disease worldwide and demands new therapeutic approaches capable of targeting intracellular Mycobacterium tuberculosis. Using a machine learning (ML)-guided design strategy based on the plectasin scaffold, a peptide library was generated and DC05 was identified as the most active analog. As a free peptide, DC05 inhibited the H37Rv strain with an MIC90 of 12.46 ± 0.06 µm, displayed low cytotoxicity in MRC-5 fibroblasts (IC50 >250 µm) and J774A.1 macrophages (115.18 ± 8.49 µm), and increased ethidium bromide accumulation to levels comparable to verapamil, …

Kaempferol and curcumin as fluorescent probes for DNA topologies: Integrated spectroscopic and computational study.

Kaempferol (3,4',5,7-tetrahydroxyflavone, KMP) and curcumin (diferuloylmethane, CUR) are naturally occurring polyphenolic compounds with bro … n C4, and moderate in duplex DNA, where anion formation was less favored. Displacement assays using ethidium bromide (EtBr) provided functional insight into the competitive binding dynamics, confirming groove and loop binding for both ligands in G4 and C4 DNA, while KMP also exhibited intercalative binding in duplex DNA. Molecular docking and molecular dyn …

Терапевтическая эффективность 4-гидроксизолеуцина в экспериментальной крысиной модели демиелинации: защита от нейронов с помощью ФУИ-1 и восстановления уровня ГЛП-1.

Множественный склероз (MS) — хроническое аутоиммунное нейродегенеративное состояние, вызывающее демиэлирование и повреждение аксона. Бромистый этадиум (ЭБРД) вызывает демиелионацию с помощью повреждения глиальных клеток, особенно олигодендроцитов и астроцитов. 4-гидроксизолеуцин (4-HI) обладает потенциалом нейрозащиты, регулируя ключевые маршруты подачи сигналов. Насколько нам известно, это первое исследование с оценкой нейрозащитного потенциала 4-HI в экспериментальной крысиной модели обезвоживания. ЦЕЛЬ: Цель настоящего исследования заключается в оценке нейрозащитного потенциала 4-HI в экспе

Совпадение в аннотации оригинала
BACKGROUND: Multiple Sclerosis (MS) is a chronic autoimmune neurodegenerative condition causing demyelination and axonal damage. Ethidium bromide (EBrD) induces focal demyelination by damaging glial cells, especially oligodendrocytes and astrocytes. 4-hydroxyisoleucine (4-HI) exhibits neuroprotective potential by regulating key signaling pathways. To our kno…

Novel furan chalcone modulates PHD-2 induction to impart antineoplastic effect in mammary gland carcinoma.

Normoxic inactivation of prolyl hydroxylase-2 (PHD-2) in tumour microenvironment paves the way for cancer cells to thrive under the influenc … ylindole (DAPI), 1,1',3,3'-tetraethylbenzimi-dazolylcarbocyanine iodide (JC-1), and acridine orange/ethidium bromide (AO/EB), against MCF-7 cells. 7,12-Dimethylbenz[a]anthracene (DMBA) model of mammary gland cancer was used to study the in vivo antineoplastic efficacy of screened compound. [(E)-1-(4-fluorophenyl)-3-(furan-2-yl) prop-2-en-1-one] (BBAP-7) wa …

HIF-2α/TFR1 mediated iron homeostasis disruption aggravates cartilage endplate degeneration through ferroptotic damage and mtDNA release: A new mechanism of intervertebral disc degeneration.

BACKGROUD: Iron overload is a prevalent condition in the elderly, often associated with various degenerative diseases, including interverteb … release of mtDNA. The inhibition of STING through siRNA or the reduction of mtDNA replication using ethidium bromide alleviated the degeneration of CEP chondrocytes induced by iron overload. CONCLUSION: Our study systemically explored the role of TFR1 mediated iron homeostasis in IDD and its underlying mechanisms, implying that targeting TFR1 to maintain …

Design and Syntheses of Ruthenium ENE (E = S, Se) Pincer Complexes: A Versatile System for Catalytic and Biological Applications.

This report describes the synthesis of two ruthenium(II) ENE pincer complexes (E = S, C1 and E = Se, C2) by the reaction of bis(2-(phenylcha … like binding behavior of both the complexes towards CT-DNA were studied by competitive binding with ethidium bromide (EthBr) using emission spectroscopy. In addition, the interactions of C1-C2 were also studied with bovine serum albumin (BSA) using steady state fluorescence quenching and synchronous fluorescence studies. A good stability of Ru(II) state wa …

Cu(II) complex with auxin (3-indoleacetic acid) and an aromatic planar ligand: synthesis, crystal structure, biomolecular interactions and radical scavenging activity.

A novel water soluble ternary copper(II) complex,-[Cu2(phen)2(3-IAA)2(H2O)](ClO4)2·H2O-(phen: 1,10-phenanthroline, 3-IAA: 3-indoleacetic acid), has been synthesized and characterized by elemental CHN analysis, ESI-TOF, FTIR and single-crystal X-ray diffraction techniques. Interaction of the complex with calf thymus DNA (CT-DNA) has been investigated by absorption spectral titration, ethidium bromide (EB) and Hoechst 33258 displacement assay. The interactions between the complex and bovine serum albumin (BSA) were investigated by electronic absorption and fluorescence spectroscopy methods. The …

Effect of hydroxyamines derived from lapachol and norlachol against Staphylococcus aureus strains carrying the NorA efflux pump.

Isolated substances and those organically synthesized have stood out over the years for their therapeutic properties, including their antiba … icant reduction of their MICs was observed when the substances were associated with norfloxacin and ethidium bromide, with this effect being attributed to efflux pump inhibition. Following a virtual analysis based on its structure (docking), information regarding the affinity of new ligands for the ABC efflux pump were observed, thus contributing to the un …

Proteomic Study of a Parkinson's Disease Model of Undifferentiated SH-SY5Y Cells Induced by a Proteasome Inhibitor.

UNLABELLED: Parkinson's disease (PD) is one of the most common nervous system degenerative diseases. However, the etiology of this disease remains elusive. Here, a proteasome inhibitor (PSI)-induced undifferentiated SH-SY5Y PD model was established to analyze protein alterations through proteomic study. METHODS: Cultured undifferentiated SH-SY5Y cells were divided into a control group and a group treated with 2.5 µM PSI (PSI-treated group). An methyl thiazolyl tetrazolium (MTT) assay was applied to detect cell viability. Acridine orange/ethidium bromide (AO/EB), α-synuclein immunofluorescence…

Detection and characterization of multidrug-resistant Salmonella enterica serovar Infantis as an emerging threat in poultry farms of Faisalabad, Pakistan.

AIMS: The aim of this study was the molecular identification of Salmonella enterica serovar Infantis (S. Infantis) isolated from poultry sam … %) showed the zoonotic potential of the isolates. The activity of efflux pumps was detected through Ethidium Bromide-agar method. CONCLUSIONS: Poultry could act as reservoirs of multidrug resistance Salmonella. SIGNIFICANCE AND IMPACT OF THE STUDY: We firstly report the prevalence and molecular characterization of virulence/drug resistance in S. Infantis …

Evaluation of Radioiodinated 1,4-Naphthoquinones as Necrosis Avid Agents for Rapid Myocardium Necrosis Imaging.

PURPOSE: Identifying necrotic myocardium in ischemic regions is of great importance for risk stratification and clinical decision-making. Ho … The uptake of [131I]naphthazarin (6) in necrotic muscle could be significantly blocked by excessive ethidium bromide (a typical DNA intercalator) and cold naphthazarin (6) with 63.49 and 71.96 % decline at 3 h post-injection in vivo, respectively. CONCLUSIONS: 1,4-Naphthoquinones retained necrosis avidity and [131I]naphthazarin (6) rapidly visualized necr …

Cancer-testis and melanocyte-differentiation antigen expression in malignant glioma and meningioma.

Identification of well-defined glioma-specific antigens is a crucial and necessary step in developing immunotherapy for glioblastoma multifo … ; and for MDA: tyrosinase, gp100, MELAN-A/MART-1, and TRP-2. The expression level was determined by ethidium bromide-stained agarose gel. Among malignant glioma tissue, the highest CTA and MDA expression rates were found for MAGE-3 (22%), MAGE-1 (16%), CT-7 (11%), gp100 (40%), and TRP-2 (29%). Among primary glioma cell lines, the highest levels of expressi …

Role of H- and D- MATE-type transporters from multidrug resistant clinical isolates of Vibrio fluvialis in conferring fluoroquinolone resistance.

BACKGROUND: The study seeks to understand the role of efflux pumps in multidrug resistance displayed by the clinical isolates of Vibrio fluv … ides like gentamicin, kanamycin and streptomycin. Decrease in MIC was also observed for acriflavin, ethidium bromide, safranin and nalidixic acid. SIGNIFICANCE: Increased resistance towards fluoroquinolones exhibited due to these efflux pumps from multidrug resistant clinical isolates of V. fluvialis implies that treatment procedure may become more elabor …