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B-type natriuretic peptide signal peptide (BNPsp) in patients presenting with chest pain.

OBJECTIVES: We assessed the ability of B-type natriuretic peptide signal peptide (BNPsp) to assist with the identification of patients with myocardial infarction (MI) and unstable angina pectoris (UAP). DESIGN AND METHODS: We studied 505 patients who presented to hospital within 4h of onset of chest pain suspicious of ACS. Blood samples were drawn at 0, 1, 2 and 24h from presentation and assayed for BNPsp, NT-proBNP, TnI and high sensitivity TnT. The ability of BNPsp and other markers to diagnose acute myocardial infarction (MI) and unstable angina pectoris (UAP) and predict subsequent events

Intracellular screening of a peptide library to derive a potent peptide inhibitor of α-synuclein aggregation.

Aggregation of α-synuclein (α-syn) into toxic fibrils is a pathogenic hallmark of Parkinson disease (PD). Studies have focused largely on re … lighted, together with the approach of using a semirational design and intracellular screening. The peptides can then be used as candidates for modification in drugs capable of slowing or even preventing the onset of PD.

Mechanism and kinetics of peptide partitioning into membranes from all-atom simulations of thermostable peptides.

Partitioning properties of transmembrane (TM) polypeptide segments directly determine membrane protein folding, stability, and function, and their understanding is vital for rational design of membrane active peptides. However, direct determination of water-to-bilayer transfer of TM peptides has proved difficult. Experimentally, sufficiently hydrophobic peptides tend to aggregate, while atomistic computer simulations at physiological temperatures cannot yet reach the long time scales required to capture partitioning. Elevating temperatures to accelerate the dynamics has been avoided, as this w…

PepBank--a database of peptides based on sequence text mining and public peptide data sources.

BACKGROUND: Peptides are important molecules with diverse biological functions and biomedical uses. To date, there does not exist a single, searchable archive for peptide sequences or associated biological data. Rather, peptide sequences still have to be mined from abstracts and full-length articles, and/or obtained from the fragmented public sources. DESCRIPTION: We have constructed a new database (PepBank), which at the time of writing contains a total of 19,792 individual peptide entries. The database has a web-based user interface with a simple, Google-like search function, advanced text …

Gastric motor effects of peptide and non-peptide ghrelin agonists in mice in vivo and in vitro.

BACKGROUND AND AIMS: The gastroprokinetic activities of ghrelin, the natural ligand of the growth hormone secretagogue receptor (GHS-R), prompted us to compare the effect of ghrelin with that of synthetic peptide (growth hormone releasing peptide 6 (GHRP-6)) and non-peptide (capromorelin) GHS-R agonists both in vivo and in vitro. METHODS: In vivo, the dose dependent effects (1-150 nmol/kg) of ghrelin, GHRP-6, and capromorelin on gastric emptying were measured by the 14C octanoic breath test which was adapted for use in mice. The effect of atropine, N(G)-nitro-L-arginine methyl ester hydrochlo

Antimicrobial/cytolytic peptides from the venom of the North African scorpion, Androctonus amoreuxi: biochemical and functional characterization of natural peptides and a single site-substituted analog.

The venoms of scorpions are complex cocktails of polypeptide toxins that fall into two structural categories: those that contain cysteinyl residues with associated disulfide bridges and those that do not. As the majority of lethal toxins acting upon ion channels fall into the first category, most research has been focused there. Here we report the identification and structural characterization of two novel 18-mer antimicrobial peptides from the venom of the North African scorpion, Androctonus amoreuxi. Named AamAP1 and AamAP2, both peptides are C-terminally amidated and differ in primary struc…

Synthetic peptides as probes for G protein function. Carboxyl-terminal G alpha s peptides mimic Gs and evoke high affinity agonist binding to beta-adrenergic receptors.

The molecular interfaces between Gs and the beta-adrenergic receptor were investigated using synthetic peptides corresponding to various regions of its alpha subunit, alpha s. These experiments were carried out on saponin-permeable C6 glioma cells in which the beta-adrenergic receptor appears tightly coupled to Gs. Synthetic site-specific peptides from alpha s (corresponding to amino acids 15-29, 354-372, and 384-394) and alpha i (8-22, 315-324, and 345-455) were tested for their ability to interfere with coupling between the beta-adrenergic receptor and Gs. The two carboxyl-terminal peptides …

Zika Virus NS2B-NS3 Протеза: Quantum Chemical Insights to Designing Inhibitory Peptides.

СПРАВОЧНАЯ ИНФОРМАЦИЯ: лихорадка " Зика " затрагивает малоимущие и уязвимые группы населения, представляя циклы, наблюдаемые по меньшей мере в 86 странах, без предупреждения или лечения вакцинами. Известно, что род Флавивирус вызывает Зика Вирус (ZIKV), как Денге и Желтая лихорадка, чьи генетические материалы декодируют, среди прочих белков, ряд неструктурных (NS) белков, необходимых для вирусного воспроизведения, таких как протеза NS2B-NS3. Кроме того, химические и биологические системы, как правило, изучаются с использованием молекулярных моделей, позволяющих, в частности, выявить

Совпадение в аннотации оригинала
BACKGROUND: Zika fever affects poor and vulnerable populations, presenting cycles observed in, at least 86 countries, with no vaccine preven … y, this study aimed to present computational docking simulations of five previously reported active peptides against NS2B-NS3 protease of ZIKV and analyze some quantum chemical properties to identify the main contribution to improving the action. METHODS: The compounds were described by Rut and coworkers (2017) and Hill and coworkers (2018), submitted to …

Gut-derived glucagon-like peptides attenuate T cell-induced intestinal injury.

Glucagon-like peptide-1 (GLP-1) and glucagon-like peptide-2 (GLP-2) are produced in enteroendocrine L cells and control energy homeostasis. … ivity in immune-mediated intestinal injury and highlight the therapeutic potential of glucagon-like peptides, alone or together, for ameliorating T cell-mediated mucosal injury.

Supramolecular Assembly System for Transdermal Penetration of Self-Assembled Short Peptides.

Transdermal delivery of peptides and proteins remains a challenge in the field of drug delivery. In this study, we successfully prepared a series of bio-based ionic liquids by proton exchange reaction adopting green-sourced organic acids and alkaloids as precursors. In addition, we constructed a supramolecular assembly system by taking advantage of self-assembled short peptides (specifically, a custom amphiphilic sequence RRIIIIRR). We also optimized the basic properties of the ionic liquid by a combination of electron microscopy and simulation computation techniques, and the transdermal absor…

Cell Penetrating Thyclotides Facilitate Efficient Delivery of Bioactive Peptides into Cells.

Cell penetrating thyclotides (CPTs) are synthetic molecules that promote highly efficient cellular uptake and endosomal escape of bioactive peptides. While peptides are valuable as medicinal agents, their translation to therapies is often limited by their inability to cross cell membranes. CPTs have a unique combination of chiral tetrahydrofurans and polar sidechains within a molecular scaffold that can be optimized to efficiently deliver peptide cargo into cells. The cellular uptake and endosomal escape of two peptides with anticancer biological activities but low bioavailabilities were remar…

Substituent-Based Modulation of Self-Assembly and Immunogenicity of Amphipathic Peptides.

Self-assembled peptide-based biomaterials provide versatile platforms for biomedical uses, featuring customizable physicochemical properties, biocompatibility, and dynamic capabilities. This self-assembly process is primarily dictated by primary sequence features, such as hydrophobicity, length, and charge, leading to the formation of fibrils and hydrogels. Amphipathic peptides, with alternating polar and hydrophobic residues, are especially effective in forming supramolecular nanofibers stabilized by π-π interactions and hydrogen bonds. Chemical modifications on aromatic side chains are promi…

Directed evolution of Clec12a-binding peptides using mammalian-expressed recombinant protein.

Clec12a is an immunoregulatory C-type lectin receptor expressed on dendritic cells as well as other myeloid cells, and involved in maintaini … asmic reticulum-Golgi pathway. Using ribosome display with mClec12a as bait, three mClec12a binding peptides (CBP1-3) were isolated, with CBP2 showing the highest affinity (K D ≈ 6.29 μM). Importantly, CBP2 bound to primary dendritic cells derived from mouse spleen and bone marrow, supporting the native-like conformation and functional integrity of the rec …

Antifungal peptides: From modes of action to synergistic and immunologic potential.

Fungal infections pose a significant global health threat with rising morbidity and mortality rates. However, the repertoire of effective antifungal drugs remains narrow, a challenge that is further exacerbated by the increasing emergence of (multi)drug-resistant strains. This underscores the urgent need for novel therapeutic strategies. Among them, antifungal peptides (AFPs) have emerged as a promising alternative. AFPs are small, naturally occurring peptides produced by a wide range of organisms, including plants, animals, fungi, and bacteria, as part of their innate immune defense. In addit…

Bioactive Peptides from Soft-Shelled Turtle: Extraction, Preparation and Biological Activities.

Background: Soft-shelled turtle (Pelodiscus sinensis), traditionally used for both medicinal and culinary purposes, is rich in high-quality protein and amino acids, making it an excellent raw material for the preparation of bioactive peptides. In recent years, soft-shelled turtle peptides have gradually become a research focus due to their significant physiological functions, including antioxidant, immunomodulatory, and anti-fatigue activities. Methods: This review systematically sorts out and analyzes 76 relevant research papers published from 2002 to 2026 and comprehensively elaborates on th…

Electrochemical Desilylative Fluorination Enables Access to N-Monofluoromethyl Amides and Peptides.

N-Fluoroalkyl amides have emerged as highly attractive targets because N-fluoromethylation can modulate conformation, stability, lipophilicity, and molecular recognition. However, despite the rapidly advancing chemistry of N-CF3 and N-CF2H amides, access to the corresponding N-monofluoromethyl (N-CH2F) amides remains significantly underdeveloped. Existing approaches are often constrained by the use of silver-based fluorinating reagents, unstable intermediates, and limited compatibility with peptide substrates. In this study, we report an electrochemical strategy for the synthesis of N-monofluo

Natriuretic peptides are integral in reclassifying heart failure stage and risk.

We investigated the usefulness of B-type natriuretic peptides (BNP) and N-terminal pro-BNP (NT-proBNP) in reclassifying individuals from Stage A heart failure (at risk of HF) to Stage B HF (pre-HF) using a database of global electronic healthcare records. Individuals with Stage A HF, defined as having at least one risk factor for HF (hypertension, atherosclerotic cardiovascular disease, diabetes mellitus, or obesity), and clinically measured BNP or NT-proBNP between 2010 and 2019 were analyzed retrospectively. The cut-offs for reclassifying Stage A to B HF were 35 pg/mL for BNP and 125 pg/mL f…

Poly(glutamic acid-block-tyrosine) peptides designed for gastrointestinal drug adsorption.

Drug overdose remains a major public health challenge, particularly for non-opioid substances that lack effective antidotes and are often treated through gastrointestinal decontamination with activated charcoal. While activated charcoal is broadly effective, its use is associated with aspiration risks and gastrointestinal complications. Here, we report a class of synthetic poly(glutamic acid-block-tyrosine) (EY) peptide adsorbent materials designed as biocompatible, tuneable alternatives for gastrointestinal drug adsorption. EY polypeptides were synthesized via N-carboxyanhydride ring-opening …

Overcoming multidrug resistance: antimicrobial peptides as a next-generation therapeutic strategy.

Amidst the escalating global threat of antibiotic resistance, there is an urgent need for novel antibacterial agents with distinct mechanisms of action to address the impending post-antibiotic era. Antimicrobial peptides (AMPs), membrane-active peptides characterized by rapid bactericidal effects, broad-spectrum activity, and low resistance development potential, are considered promising candidates for overcoming the current multidrug resistance (MDR) crisis. However, the clinical application of AMPs is significantly limited by inherent drawbacks, including susceptibility to proteolytic degrad…