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Regulation of perineuronal net components in the synaptic bouton vicinity on lumbar α-motoneurons in the rat after spinalization and locomotor training: New insights from spatio-temporal changes in gene, protein expression and WFA labeling.

Chondroitin sulfate proteoglycans (CSPGs) consist of core proteins and glycosaminoglycan side chains. Tenascins, and hyaluronan and proteoglycan link protein 1 (HAPLN), link CSPGs with a hyaluronan backbone to constitute perineuronal nets (PNNs), which ensheath preferentially highly active neurons to maintain architecture and stabilize synapses, but restrict repair plasticity. Spinal cord injury increases CSPG core protein levels in the lesion proximity, limiting permissiveness of the extracellular milieu for fiber regrowth, however regulation of PNNs structure in the vicinity of distant α-mot…

In Vitro Model Systems of Coxsackievirus B3-Induced Myocarditis: Comparison of Commonly Used Cell Lines and Characterization of CVB3-Infected iCell® Cardiomyocytes.

Coxsackievirus B3 (CVB3) belongs to the enteroviruses, which are a well-known cause of acute and chronic myocarditis, primarily infecting ca … he expression of cell death markers were compared. The various cell lines clearly differed in their permissiveness to CVB3 infection, patterns of infection, viral load, and mode of cell death. When studying the mode of cell death of CVB3-infected iCell® Cardiomyocytes in more detail, especially regarding the necroptosis key players RIPK1 and RIPK3, we found that …

SARS-CoV-2 host tropism: An in silico analysis of the main cellular factors.

Recent reports have shown that small and big felines could be infected by SARS-CoV-2, while other animals, like swines and mice, are apparen … ad by acting as secondary hosts (cats, dogs, pigs, mice, and ferrets) and evaluated their predicted permissiveness by in silico analysis. Results showed that ionic pairs (salt bridges, N-O pair, and long-range interactions) produced between ACE2 and the viral spike has an essential function in the host interaction. On the other hand, TMPRSS2 and GRP78 are protei …

The second European interdisciplinary Ewing sarcoma research summit--A joint effort to deconstructing the multiple layers of a complex disease.

Despite multimodal treatment, long term outcome for patients with Ewing sarcoma is still poor. The second "European interdisciplinary Ewing … h a focus on EWS-FLI1. Data were presented shedding light on the molecular underpinnings of genetic permissiveness to this disease uncovering interaction of EWS-FLI1 with recently discovered susceptibility loci. Epigenetic context as a consequence of the interaction between the oncoprotein, cell type, developmental stage, and tissue microenvironment emerged as d …

The Sphingosine-1-Phosphate Lyase (LegS2) Contributes to the Restriction of Legionella pneumophila in Murine Macrophages.

L. pneumophila is the causative agent of Legionnaires' disease, a human illness characterized by severe pneumonia. In contrast to those deri … hages (hMDMs) are more permissive to L. pneumophila legS2 mutants than wild-type (WT) strains. This permissiveness to L. pneumophila legS2 is neither attributed to abolished caspase-1, caspase-7 or caspase-3 activation, nor due to the impairment of phagosome-lysosome fusion. Instead, an infection with the legS2 mutant resulted in the reduction of some inflammato …

Enteric-delivered rapamycin enhances resistance of aged mice to pneumococcal pneumonia through reduced cellular senescence.

Rapamycin, a potent immunomodulatory drug, has shown promise in the amelioration of numerous age-associated diseases including cancer, Alzhe … or during pneumococcal pneumonia. Previously we have demonstrated that cellular senescence enhances permissiveness for bacterial pneumonia through increased expression of the bacterial ligands Laminin receptor (LR), Platelet-activating factor receptor (PAFr) and Cytokeratin 10 (K10). These proteins are co-opted by S. pneumoniae and other respiratory tract pathog …

Contribution of rearranged actin structures to the spread of Ectromelia virus infection in vitro.

We describe here a contribution of virus-induced actin tails and filopodia in transmission of Ectromelia virus (ECTV) infection in permissive cells detected by the immunofluorescence and confocal microscopy. Immunoblot analysis revealed profoundly decreased beta-actin levels during ECTV replicative cycle in the infected cells 24 hrs post infection (p.i.). These results provided a basis for the further analysis of ECTV motion in the infected cells as well as for impact of ECTV infection on the cytoskeletal proteins.

The role of contextual factors, school-related attitudes and behavioural traits on lifetime antisocial behaviours among adolescents.

INTRODUCTION: Antisocial behaviour is a result of a multifactorial process that involves both social-level and individual-level factors. The … 39% and of three or more antisocial behaviours 21.8%. Among contextual factors, perceived parental permissiveness to use substances, low perceived parental support, low perceived class climate, and perceptions of peer's cigarette, alcohol and illicit drug use were associated with higher odds of antisocial behaviour. Low pupil's respect for teacher, high impulsi …

Attitudes Toward Suicide Are Associated With Lifetime History of Depressive Symptoms and Suicide Attempts Among Entertainment Industry Workers in South Korea.

OBJECTIVE: Entertainment industry workers (EIWs) face unique psychosocial stressors, yet systematic research on the psychological contributo … R=4.95, 95% CI: 1.88-17.10; aOR=2.67, 95% CI: 1.44-4.96, respectively). Regarding ATTS-20 factors, "permissiveness" and "unpredictability" were significantly associated with lifetime history of depressive symptoms (aOR=1.67, 95% CI: 1.22-2.30; aOR=1.48, 95% CI: 1.12-1.97, respectively) in multivariable analysis. Notably, the "relation-caused" factor (believing s …

Developmentally constrained plasticity as a therapeutic vulnerability in cancer.

Cancer cells can exploit developmental lineage programs to generate phenotypic heterogeneity under therapeutic pressure. Although a subset o … nder therapy. We distinguish physiological expansion of state space during repair from premalignant permissiveness and malignant fixation, and classify resistance into three modes: lineage-maintained resistance, adaptive reversible plasticity, and fixed reprogramming through lineage switching or histological transformation. These modes should be read as diagnose …

Acupuncture for Poststroke Dysphagia: An NTS-Centered Hypothesis-Generating Framework Linking the Brainstem Swallowing Network, Neuroimmune Regulation, and Vagal Anti-Inflammatory Signaling.

BACKGROUND: Poststroke dysphagia (PSD) may reflect not only focal motor pathway injury but also instability of the brainstem swallowing netw … k recovery by modifying inflammatory burden, glial state, autonomic balance, and microenvironmental permissiveness. However, most mechanistic evidence is derived from ischemic stroke models in which swallowing was not the primary outcome. CONCLUSION: We propose an NTS-focused, hypothesis-generating framework in which acupuncture may support PSD recovery by modi …

Trends in lifetime controlled drug use and associated risk factors among Japanese Junior High School Students: Findings from Nationwide Surveys, 2016-2024.

AIM: To examine trends in the lifetime prevalence of controlled drug use among Japanese junior high school students (aged 12-15 years) betwe … in 2024. Similar downward trends were observed for all substances. While perceived drug access and permissiveness declined, tolerant attitudes toward marijuana slightly increased. Invitations to use drugs also became more common. Risk factors for controlled drug use included male sex, alcohol and tobacco use, permissive attitudes, drug invitations, irregular ro …

Neuroteratogenic mechanisms of Zika virus (ZIKV) infection: Insights into fetal brain development disruption and congenital Zika syndrome: A systematic review.

The Zika virus (ZIKV), a mosquito-borne Flavivirus, has emerged as a global health threat due to its severe neuroteratogenic effects, partic … rodevelopmental impairment-even in normocephalic infants. Importantly, ZIKV exhibits SOX2-dependent permissiveness with integrin αvβ5 functioning as a key dependency/attachment factor in neural and glioblastoma stem cells, distinguishing transcriptional state from receptor function. This intersection of neurotropism and potential oncolytic activity underscores t …

Phenotypic variation and vector competence in Anopheles aquasalis: A comparative analysis of susceptibility to Plasmodium vivax.

Malaria remains a major public health problem worldwide, with Plasmodium vivax being the predominant species in the Americas. Anopheles aqua … nces were similar, 30BS mosquitoes harbored significantly higher parasite loads, suggesting greater permissiveness to parasite development. These findings highlight the importance of considering intraspecific variation in malaria vector competence and its potential implications for transmission dynamics and control strategies.

Predominance of Leishmania (Leishmania) amazonensis DNA in Lutzomyia longipalpis sand flies (Diptera: Psychodidae) from an endemic area for leishmaniasis in Northeastern Brazil.

Leishmaniasis is a serious public health concern in the Northeastern region of Brazil, where the sand fly fauna is well studied, although fe … though Lu. longipalpis is the vector of L. (L.) infantum, which was not detected in this study, its permissiveness for the transmission of L. (L.) amazonensis has been reported. We suspect that visceral leishmaniasis and cutaneous leishmaniasis are caused by L. (L.) amazonensis, and the transmission may be occurring through Lu. longipalpis, at least in the urban …

Splenic macrophages as the source of bacteraemia during pneumococcal pneumonia.

BACKGROUND: Severe community-acquired pneumococcal pneumonia is commonly associated with bacteraemia. Although it is assumed that the bacteraemia solely derives from pneumococci entering the blood from the lungs it is unknown if other organs are important in the pathogenesis of bacteraemia. Using three models, we tested the relevance of the spleen in pneumonia-associated bacteraemia. METHODS: We used human spleens perfused ex vivo to explore permissiveness to bacterial replication, a non-human primate model to check for splenic involvement during pneumonia and a mouse pneumonia-bacteraemia mo…

Efficient acute and chronic infection of stem cell-derived hepatocytes by hepatitis C virus.

OBJECTIVE AND DESIGN: Human stem cell-derived hepatocyte-like cells (HLCs) have shown high potential as authentic model for dissection of the HCV life cycle and virus-induced pathogenesis. However, modest HCV replication, possibly due to robust innate immune responses, limits their broader use. To overcome these limitations and to dissect the mechanisms responsible for control of HCV, we analysed expression of key components of the interferon (IFN) system in HLCs, assessed permissiveness for different HCV strains and blocked innate immune signalling by pharmacological intervention. RESULTS: T…

Сопоставление репликации вируса лихорадки Рифт-Вэлли на североамериканском скотоводстве и линиях клеток дикой природы.

Вирус лихорадки Рифт-Вэлли (RVFV) вызывает вспышки заболеваний по всей Африке и Аравийскому полуострову, что приводит к высокой заболеваемости и смертности среди молодого домашнего скота, частым абортам у беременных животных и потенциально тяжелым или смертельным заболеваниям у людей. Возможность распространения ОВТС на Соединенные Штаты или другие страны во всем мире вызывает серьезную озабоченность в отношении животных и здоровья населения, животноводства и торговли. Механизм стойкости ОВТС в межэпидемические периоды может осуществляться путем трансовариальной передачи комаров и/или с помощь

Совпадение в аннотации оригинала
Rift Valley fever virus (RVFV) causes disease outbreaks across Africa and the Arabian Peninsula, resulting in high morbidity and mortality a … s a variety of cell lines derived from North American livestock and wildlife for susceptibility and permissiveness to RVFV. Results of this study suggest that RVFV could potentially replicate in native deer species such as white-tailed deer, and possibly a wide range of non-ruminant animals. This work serves to guide and support future animal model studies and r …

Multi-step regulation of interferon induction by hepatitis C virus.

Acute hepatitis C virus (HCV) infection evokes several distinct innate immune responses in host, but the virus usually propagates by circumventing these responses. Although a replication intermediate double-stranded RNA is produced in infected cells, type I interferon (IFN) induction and immediate cell death are largely blocked in infected cells. In vitro studies suggested that type I and III IFNs are mainly produced in HCV-infected hepatocytes if the MAVS pathway is functional, and dysfunction of this pathway may lead to cellular permissiveness to HCV replication and production. Cellular immu…

Ethnoepidemiology of HTLV-1 related diseases: ethnic determinants of HTLV-1 susceptibility and its worldwide dispersal.

Human T-cell lymphotropic virus type 1 is vertically transmitted in neonatal life and is causatively associated with adult T-cell leukemia ( … ng on genetics of the human leukocyte antigen (HLA) that determines the ethnic background of HTLV-1 permissiveness and susceptibility to ATL or HAM/TSP. The serological surveillance revealed transcontinental dispersal of HTLV-1 in the prehistoric era that started out of Africa, spread to Austro-Melanesia and the Asian continent, then moved to North America and t …