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Carabrone alleviates neuroinflammation by covalently targeting ubiquitin conjugating enzyme UBE2D3 in experimental autoimmune encephalomyelitis.

Multiple sclerosis (MS) is an incurable and progressive inflammatory disease of the central nervous system (CNS) characterized by inflammatory demyelination, chronic axonal damage, and neurodegeneration. Carabrone (CA), a sesquiterpene lactone from the traditional Chinese herb Carpesium abrotanoides L., demonstrates diverse pharmacological properties. However, its therapeutic effects on MS and the underlying mechanisms remain unexplored. In the present study, we reported the beneficial effects of CA on experimental autoimmune encephalomyelitis (EAE), a well-established mouse model of MS, for t…

PEPITEM Reduces Annexin V Immunoreactivity in Spinal Cord Tissue from Experimental Autoimmune Encephalomyelitis Mice.

INTRODUCTION: Multiple sclerosis is a chronic autoimmune demyelinating disease of the central nervous system, and experimental autoimmune encephalomyelitis is widely used as a murine model to study its immunopathological features. PEPITEM regulates leukocyte trafficking; however, its effect on apoptosis-associated tissue changes in EAE remains unclear. This study aimed to determine whether prophylactic or therapeutic administration of PEPITEM alters Annexin V immunoreactivity in spinal cord sections from EAE mice. METHODS: EAE was induced in female C57BL/6 mice using MOG35-55 immunization. Mi…

Possible Racial Disparities in the Diagnosis of Myalgic Encephalomyelitis/Chronic Fatigue Syndrome (ME/CFS).

Myalgic encephalomyelitis (ME/CFS) a chronic, disabling illness with no established etiopathology. It has been indicated in some population-based studies that Black and ethnic minority populations are underdiagnosed with ME/CFS. The aims of the present study were to (1) identify the agreement between receiving an ME/CFS diagnosis and meeting diagnostic criteria, (2) identify the demographic characteristics associated with receiving a diagnosis, and (3) explore patient satisfaction with healthcare. Self-reported medical history and symptoms were collected via online survey from respondents with…

How a Clinician Makes a Diagnosis for Myalgic Encephalomyelitis/Chronic Fatigue Syndrome (ME/CFS).

This chapter describes how a clinician with experience of myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS) makes a diagnosis of the condition in the absence of a routine laboratory diagnostic test.

Regulatory B cell CCL3 competency promotes disease resolution and oligodendrogenesis in experimental autoimmune encephalomyelitis.

Regulatory B cells (Bregs) contribute to immune homeostasis via IL-10-dependent and independent pathways. To dissect additional mediators, we investigated splenic derived GM-CSF/IL-15 fusokine (GIFT15)-induced Bregs in experimental autoimmune encephalomyelitis (EAE) using transcriptomics and functional validation. Bulk RNA-seq of splenic Bregs revealed upregulation of Ccl3, GzmB, and Il27 subunits compared to resting B cells. Functional studies showed that CCL3-deficient Bregs failed to suppress disease, whereas GzmB-deficient Bregs retained efficacy, and IL-27 receptor signaling in recipients…

Burden of Disease in Myalgic Encephalomyelitis/Chronic Fatigue Syndrome (ME/CFS): A Scoping Review.

OBJECTIVE: Myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS) is a serious chronic and complex multi-system disease characterised by symptoms such as post-exertional malaise, fatigue, cognitive impairment and pain. Diagnosis is based on international consensus criteria, and no curative treatment is available. In the USA, its prevalence is estimated at 0.42% among adults, with women affected three times as often as men. Prevalence is expected to increase due to the COVID-19 pandemic. In addition to its severe symptoms, ME/CFS has a substantial economic impact. This scoping review aimed…

Acute disseminated encephalomyelitis mimicking brain tumor: about 2 pediatric cases: ADEM mimicking brain tumor.

INTRODUCTION: Acute disseminated encephalomyelitis (ADEM) ADEM is a well-known and treatable inflammatory pediatric condition with a good prognosis outcome. Atypical forms exist that can lead to misdiagnosis and sometimes inappropriate management. OBSERVATION AND DISCUSSION: We report two cases with tumor-like lesions, highlighting their clinical and magnetic resonance imaging (MRI) features. Diagnostic keys for prompt recognition are discussed. CONCLUSION: Tumefactive demyelinating lesions can mimic brain tumors or abscesses, leading to potential misdiagnosis and inappropriate or delayed tr…

Psychosocial Nursing Diagnoses of Individuals With Myalgic Encephalomyelitis-Chronic Fatigue Syndrome: A Descriptive Study.

AIM: To describe the prevalence of psychosocial nursing diagnostic labels and their relationship with sociodemographic characteristics in adults with myalgic encephalomyelitis-chronic fatigue syndrome (ME/CFS). DESIGN: This is a cross-sectional descriptive study. METHODS: Population: Adults with ME/CFS. INCLUSION CRITERIA: Being 18 years of age or older, having a medical diagnosis of ME/CFS and being an active member of a patient association. Data collection took place between May and July 2022 using an online and paper-based ad hoc form that included sociodemographic and clinical data. Psy…

Гут Микробиом и мальгический энцефаломилит/хронический синдром утомления (ME/CFS): Понимание механизмов борьбы с заболеваниями.

Myalgic Encephalomyelitis/Chronic Fatige (ME/CFS) — клиническая болезнь, характерная особенность которой заключается в постэкспертальной болезни (PEM). Она может влиять на многие органы и системы, что приводит к серьезному ухудшению качества жизни пациентов. Несмотря на многочисленные постинфекционные, иммунологические, неврологические, метаболические и эндокринные изменения, не существует ни окончательного диагностического маркера, ни утвержденного лечения. Этиология и патофизиология по-прежнему не до конца понимаются; однако появляющиеся данные свидетельствуют о том, что кишечная микробиомия…

Терапевтический потенциал ингибитора редукции S-нитрозоглутатиона в экспериментальном аутоиммунном энцефаломиелите с помощью клеток B.

Ранее мы сообщали о том, что S-нитрозоглотатиона (GSNO) и GSNO ингибиторы редукции, которые увеличивают эндогенную GSNO, смягчают экспериментальный аутоиммунный энцефаломиелит (EAE) (животная модель множественного склероза) путем подавления клеток T1 и T17 эффектора Т при одновременном поощрении регулирования Т клеток (Tregs). В этом исследовании мы продемонстрировали, что ингибитор редукции GSNO (N6022) также уменьшает зависимость EAE от клеток в C57BL/6 мышах, иммунизированных рекомбинантным человеком milelin oligodendrocyte glycoprotein (rhMOG1-125). Ежедневное лечение N6022 после заболеван

Совпадение в аннотации оригинала
We previously reported that S-nitrosoglutathione (GSNO) and GSNO reductase inhibitors, which increase endogenous GSNO, mitigate T cell-dependent experimental autoimmune encephalomyelitis (EAE), an animal model of multiple sclerosis, by suppressing Th1 and Th17 effector T cells while promoting regulatory T cells (Tregs). In this study, we demonstrate that the…

The Quinazoline Derivative, QNZ, Alleviates Experimental Autoimmune Encephalomyelitis by Suppressing Th1 and Th17 Cells.

AIMS: Multiple Sclerosis (MS) is a neuroinflammatory and neurodegenerative disease affecting the central nervous system (CNS). Substantial e … assessed the impact of QNZ on CD4+ T-cell polarization in MS. Utilizing the experimental autoimmune encephalomyelitis (EAE) model, we investigated these aspects of MS. METHOD: EAE was induced in C57BL/6 female mice by active immunization with myelin oligodendrocyte glycoprotein (MOG)35-55 peptide. QNZ was injected intraperitoneally (i.p.) once every 2 days after t …

Раннее воздействие мышей NOD/ShiLtJ на аппендициты Фрэунда отсрочено, спонтанно прогрессирующий энцефаломиэлит.

Наркотики, способные эффективно противодействовать первичному прогрессивному НР (ПП-МС), остаются неудовлетворенной потребностью. Наличие надежных моделей PP-MS для животных может способствовать выявлению методов лечения, способных противодействовать эволюции заболеваний. Недавно мы описали первичную прогрессивную EAE (PP-EAE) в мышах NOD/ShiLtJ, показав, что она резюмирует несколько ключевых особенностей РРМС. Вместе с тем фундаментальное различие между РРМС и ИПОЕ заключается в том, что последние возникают в результате потери допусков, преднамеренно вызванной периферийным расширением специфи

Совпадение в аннотации оригинала
Drugs able to efficiently counteract primary progressive MS (PP-MS) remain an unmet need. The availability of reliable animal models of PP-M … developed spontaneous PP-EAE (SPP-EAE). Specifically, we report that the sole CFA challenge induced encephalomyelitis with a similar pattern of that prompted by the complete immunization protocol including CFA, pertussis toxin and MOG35-55. Mice with SPP-EAE show primary progressive disease evolution, widespread neurodegeneration, and insensitivity to dexamethasone …

Carnosol and melatonin co-treatment attenuates neuroinflammation and oxidative stress in experimental autoimmune encephalomyelitis.

INTRODUCTION: Neurodegenerative diseases such as multiple sclerosis (MS) involve neuronal loss and functional impairment driven by inflammation and oxidative stress. Carnosol and melatonin are recognized for their anti-oxidative properties and potential to support remyelination. This study assessed the combined therapeutic effect of Carnosol and melatonin in the experimental autoimmune encephalomyelitis (EAE) model. MATERIALS AND METHODS: EAE was induced in 28 female C57BL/6 mice, which were divided into four groups: Control, Carnosol (50 mg/kg), melatonin (10 mg/kg), and combination treatmen…

Yerba Mate (Ilex paraguariensis) Ameliorates Experimental Autoimmune Encephalomyelitis by Modulating Regulatory T Cell Function.

Background/Objectives: In Latin America, yerba mate (YM) is a popular infusion processed from the leaves and stems of Ilex paraguariensis. YM has been shown to have anti-inflammatory properties in several studies, although the effect of YM on multiple sclerosis (MS) remains elusive. The purpose of this study was to examine the effect of YM on the development of MS, by using the experimental autoimmune encephalomyelitis (EAE) mouse model while also evaluating its effect over infiltration of immune cells into the central nervous system (CNS) and regulatory T cell (Treg) function. Methods: YM or …

Иммуномодулирующий потенциал токсоплазмы гонди лизата в C57BL/6 модели мыши аутоиммунного энцефаломиелита.

Множественный склероз (MS) — изнурительное аутоиммунное заболевание, характеризующееся хроническим воспалением и обезвоживанием в центральной нервной системе. Иммунотерапия является одним из важнейших элементов борьбы с симптомами и прогрессированием MS. Цель этого исследования состояла в том, чтобы оценить иммуномуляторное воздействие токсоплазмы гонди лизат антигенов (ТЛА) в модели мыши C57BL/6 экспериментального аутоиммунного энцефаломиелита (EAE). Восемнадцать мышей были произвольно отнесены к трем группам: здоровый контроль, мыши, вызванные EAE, и мыши, вызванные EAE, обработанные TLA. Еж

Совпадение в аннотации оригинала
Multiple sclerosis (MS) is a debilitating autoimmune disease characterized by chronic inflammation and demyelination within the central nerv … ects of Toxoplasma gondii lysate antigens (TLA) in a C57BL/6 mouse model of experimental autoimmune encephalomyelitis (EAE). Eighteen mice were randomly assigned into three groups: healthy controls, EAE-induced mice, and EAE-induced mice treated with TLA. Clinical scores were recorded daily for all mice. To assess immune responses, mRNA and protein expression level …

Иммуно-питательная терапия в экспериментальном аутоиммунном энцефаломиелите: перевод в систему управления множественным склерозом.

Множественный склероз (MS) — хроническое аутоиммунное расстройство, характеризующееся дегенерацией миелин в центральной нервной системе (CNS), приводящее к значительным неврологическим расстройствам. Поражает примерно 2,8 миллиона человек во всем мире и имеет многофакторную аэтологию, связанную с генетической предрасположенностью и экологическими факторами, в частности с влиянием на питание. В настоящем обзоре рассматривается новая область иммунотерапии в качестве новаторского подхода к регулированию экспериментального аутоиммунного энцефаломиелита (EAE), широко признанного животного образца M

Совпадение в аннотации оригинала
Multiple sclerosis (MS) is a chronic autoimmune disorder characterised by myelin degeneration in the central nervous system (CNS), leading t … erging field of immuno-nutritional therapy as a novel approach for managing experimental autoimmune encephalomyelitis (EAE), a widely accepted animal model of MS. We highlight the therapeutic potential of key nutritional components, such as omega-3 fatty acids, polyphenols, and vitamins A and D, which have been shown to modulate immune responses and promote neuropr …

Occurrences of avian encephalomyelitis virus in naturally infected chicks in Saudi Arabia's Eastern Province.

BACKGROUND: A neurological infectious viral disease, avian encephalomyelitis was initially discovered in 2-week-old commercial chicks in 1930 and classified as a neurotropic viral disease. AIM: A neurological outbreak caused by avian encephalomyelitis virus (AEV) in young chicks was first reported in Al-Ahsa in the Kingdom of Saudi Arabia (KSA) in 2010. The aim of this article is to examine the AEV in KSA, Al-Ahsa Province. METHODS: Gizzard, proventriculus, cerebrum, cerebellum, and medulla oblongata tissue samples were collected from infected chicks for histopathology test and molecular ide…

Central 5-HTergic hyperactivity induces myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS)-like pathophysiology.

OBJECTIVES: Myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS) is a significant medical challenge, with no indisputable pathophysiological mechanism identified to date. METHODS: Based on clinical clues, we hypothesized that 5-hydroxytryptamine (5-HT) hyperactivation is implicated in the pathogenic causes of ME/CFS and the associated symptoms. We experimentally evaluated this hypothesis in a series of mouse models. RESULTS: High-dose selective serotonin reuptake inhibitor (SSRI) treatment induced intra- and extracellular serotonin spillover in the dorsal raphe nuclei of mice. This co…

Transcriptional reprogramming primes CD8+ T cells toward exhaustion in Myalgic encephalomyelitis/chronic fatigue syndrome.

Myalgic encephalomyelitis/chronic fatigue syndrome (ME) is a severe, debilitating disease, with substantial evidence pointing to immune dysregulation as a key contributor to pathophysiology. To characterize the gene regulatory state underlying T cell dysregulation in ME, we performed multiomic analysis across T cell subsets by integrating single-cell RNA-seq, RNA-seq, and ATAC-seq and further analyzed CD8+ T cell subpopulations following symptom provocation. Specific subsets of CD8+ T cells, as well as certain innate T cells, displayed the most pronounced dysregulation in ME. We observed upreg…