Результаты поиска

Effects of thyroid and luteinizing hormones on the onset of precursor cell differentiation into leydig progenitor cells in the prepubertal rat testis.

Leydig cells in the adult rat testis differentiate during the neonatal-prepubertal period. However, the stimulus for the initiation of their … s (LHR) in testicular interstitial cells (other than the fetal Leydig cells) was observed using the avidin-biotin method. In control rats, out of all spindle-shaped cell types in the testis interstitium, only the peritubular mesenchymal cells showed positive immunolabeling for 3beta-HSD, beginning from the postnatal Day 11. However, positive immunolabeli …

Immunohistochemical characterization of calcitonin gene-related peptide in the trigeminal system of the familial hemiplegic migraine 1 knock-in mouse.

INTRODUCTION: Familial hemiplegic migraine type 1 (FHM-1) is caused by mutations in the CACNA1A gene, with the R192Q mutation being the most common. Elevated calcitonin gene-related peptide (CGRP) levels in acute migraine and clinical trials using CGRP receptor antagonists suggest CGRP-related mechanisms are important in migraine. METHODS: Wild-type and R192Q knock-in mice were anaesthetized and perfused. Using immunohistochemical staining, the expression of CGRP in the trigeminocervical complex (TCC) and in the trigeminal and dorsal root ganglia was characterized. RESULTS: There was a 38% r

Distribution of epidermal growth factor receptor (EGFr) in normal and acute peptic-injured equine gastric squamous epithelium.

Growth factors are important in healing and restoration of injured gastrointestinal tissues and, therefore, we characterised temporally the … plicatus along the right side of the stomach/greater curvature and the lesser curvature. A modified avidin-biotin immunoperoxidase technique was used to stain the formalin-fixed tissue specimens for EGFr. A computerised image analysis system was used to measure area occupied by EGFr (EGFr area) and mean EGFr density in 4 zones within the epithelium exten …

Concomitant mobilization of plasma cells and hematopoietic progenitors into peripheral blood of multiple myeloma patients: positive selection and transplantation of enriched CD34+ cells to remove circulating tumor cells.

One advantage of the use of peripheral blood stem cells (PBSCs) over autologous bone marrow would be a reduced risk of tumor cell contaminat … d to PBSC collection. In 10 patients, circulating hematopoietic CD34+ cells were highly enriched by avidin-biotin immunoabsorption, were cryopreserved, and used to reconstitute bone marrow function after myeloablative therapy. The median purity of the enriched CD34+ cell population was 89.5% (range, 51% to 94%), with a 75-fold increase as compared with t …

Toward the development of an innovative pharmacological compound employing peptides for EGFR-mediated drug delivery in anaplastic thyroid carcinoma.

Anaplastic thyroid cancer (ATC) represents the deadliest thyroid tumor in humans. Current treatments have not really demonstrated a long-ter … eviations: 7-AAD, 7-Aminoactinomycin D; AA%, amino acid composition expressed as a percentage; ABC, Avidin/Biotinylated enzyme Complex; ABTS, 2,2'-azino-bis-3-ethylbenzothioazoline-6-sulfonic acid; A.I., aliphatic index; AKT, protein kinase B; ALT, alanine aminotransferase; AST, aspartate aminotransferase; ATC, anaplastic thyroid carcinoma; Bad, the Bcl2 …

Concomitant mobilization of plasma cells and hematopoietic progenitors into peripheral blood of patients with multiple myeloma.

In this article, we review neoplastic contamination in the peripheral blood (PB) of patients with multiple myeloma (MM) upon stem cell mobil … prior to autologous transplantation, circulating hematopoietic CD34+ cells were highly enriched by avidin-biotin immunoabsorption, cryopreserved, and used to reconstitute bone marrow (BM) function after myeloablative therapy in 13 patients. The median purity of the enriched CD34+ cell population was 89.5% (range 51%-94%), with a 75-fold enrichment compa …

Advanced Diagnostic Techniques

Аннотация не предоставлена источником. Откройте карточку для перехода к публикации.

Совпадение в полном тексте источника
… Numerous tissues have endogenous avidin-biotin activity that must be blocked before adding biotinylated reagents in avidin-biotin detection systems. … Current IHC methods can be divided into avidin-biotin or non–avidin-biotin systems. After incubation with the primary antibody, a secondary antibody specific for the primary antibody (secondary reagent) is added. For avidin-biotin systems the secondary reagent is biotinylated. …

Advanced Diagnostic Techniques

Аннотация не предоставлена источником. Откройте карточку для перехода к публикации.

Совпадение в полном тексте источника
… Numerous tissues have endogenous avidin-biotin activity that must be blocked before adding biotinylated reagents in avidin-biotin detection systems. … For avidin-biotin methods, a tertiary reagent labeled with avidin molecules and an enzyme (peroxidase or alkaline phosphatase) is needed ( Ramos-Vara and Miller, 2014 ). The most common non–avidin-biotin method is based on polymer technology. …

Endocrine cells in the oxyntic mucosa of the stomach in patients with irritable bowel syndrome.

AIM: To study the different endocrine cell types in the oxyntic mucosa of patients with irritable bowel syndrome (IBS). METHODS: Seventy-si … samples were taken from the corpus. Sections from these biopsy samples were immunostained using the avidin-biotin complex (ABC) method, for ghrelin, serotonin, somatostatin and histamine. The densities of these cell types and immunoreactivity intensities were quantified using computerized image analysis with Olympus cellSens imaging software (version 1.7 …

Understanding Mesangial Pathobiology in AL-Amyloidosis and Monoclonal Ig Light Chain Deposition Disease.

Patients with plasma cell dyscrasias produce free abnormal monoclonal Ig light chains that circulate in the blood stream. Some of them, termed glomerulopathic light chains, interact with the mesangial cells and trigger, in a manner dependent of their structural and physicochemical properties, a sequence of pathological events that results in either light chain-derived (AL) amyloidosis (AL-Am) or light chain deposition disease (LCDD). The mesangial cells play a key role in the pathogenesis of both diseases. The interaction with the pathogenic light chain elicits specific cellular processes, whi

Совпадение в полном тексте источника
… Avidin-biotin technique, diaminobenzidine as marker. Original magnification ×700. (h–j) In vivo rat model of LCDD. (h) Periodic acid–Schiff (PAS) stain showing expanded mesangial with increased PAS-positive extracellular matrix (circles). … Avidin-biotin technique, diaminobenzidine as marker. Original magnification ×700. (h–j) In vivo rat model of LCDD. (h) Periodic acid–Schiff (PAS) stain showing expanded mesangial with increased PAS-positive extracellular matrix (circles). …

Синергическое регенеративное, иммунодуляционное и антивоспалительное воздействие ваниллина и адипозы производных стволовых клеток в животной модели купризонного склероза.

ЦЕЛИ: Ваниллин как потенциальный терапевтический агент наряду с трансплантацией нейротрофических факторов, отделяющих клетки, считается перспективным средством лечения неврологических расстройств. В этом исследовании последствия ваниллина в сочетании с трансплантацией стволовых клеток, полученных человеком, были оценены с точки зрения дифференциации олигодендроцитов, повторного сглаживания и улучшения моторных функций. МАТЕРИАЛЫ И МЕТОДЫ: Женский C57BL/6 Мышей кормили кухонной зоной.400 мг/кг/сут.) 5 неделями, чтобы вызвать MS. Демилинированные мыши были разделены на группы MS, MS/vanillin, MS

Совпадение в полном тексте источника
… First of all, washing buffer (KPG-WB, karmaniaparsgen, Iran) and avidin-HRP solution (KPG-HA, karmaniaparsgen, Iran) were prepared according to the company's protocol. … Next, 50 μl of HRP-avidin solution (HAA, karmaniaparsgen, Iran) was added to all wells except the control well and placed on a shaker for 50 min (step 6). After incubation, the plates were washed 5 times (step 7) and then 50 μl of substrate was added to all wells and incubated for 15 min (step 8). …

The Emergence of Universal Immune Receptor T Cell Therapy for Cancer.

Chimeric antigen receptor (CAR) T cells have shown great success in the treatment of CD19+ hematological malignancies, leading to their recent approval by the FDA as a new cancer treatment modality. However, their broad use is limited since a CAR targets a single tumor associated antigen (TAA), which is not effective against tumors with heterogeneous TAA expression or emerging antigen loss variants. Further, stably engineered CAR T cells can continually and uncontrollably proliferate and activate in response to antigen, potentially causing fatal on-target off-tumor toxicity, cytokine release s

Совпадение в полном тексте источника
… This can either be done through the use of natural binding partners, such as avidin-biotin or leucine zippers, or through binding of an anti-tag CAR to its cognate antigen tag. … Relying upon the known interaction between biotin and avidin, the first tag-specific UIR to be developed used dimeric chicken avidin for the extracellular domain of the receptor, thereby allowing for redirection of engineered T cells against biotinylated TLs, including both scFvs and antibodies ( 52 …

Несапонин из Панакс Гинсенг поддерживает целостность мозгового барьера, подавляя NF-ИБ и p38 MAP cinase сигнализирующих путей для предотвращения развития экспериментального аутоиммунного энцефаломиэлита.

НЕСАпонинская (NS) фракция является важным активным компонентом Панакс Гинсенга, имеющей многофункциональную фармакологическую деятельность, включая нейрозащиту, иммунную регламентацию, противовоспалительные и антиоксидантные эффекты. Однако воздействие НС на множественный склероз (МС) - хроническое и аутоиммунное демиелионирующее расстройство - пока еще не доказано. Цель настоящего исследования состояла в том, чтобы продемонстрировать фармакологические действия НСУ по дисфункциям движения и соответствующие механизмы действий с использованием экспериментальной аутоиммунной энцефаломиелитной мо

Совпадение в полном тексте источника
… Biotinylated rabbit/rat/mouse IgG (BA-1000; BA-9400; BA-2000) and horseradish peroxidase (HRP)-conjugated secondary antibodies (A-2014) and avidin-biotinylated HRP-complex (A-2004, ABC) were purchased from Vector Laboratories (Burlingame, PA). … The sections were incubated avidin-biotinylated HRP-complex and visualized with 3, 3′-diamino-benzidine. Immunofluorescence staining was conducted as previously described [ 36 , 37 ]. …

Association of serum cyclooxygenase-2 levels with hand-foot syndrome in patients receiving capecitabine: an exploratory analysis of D-TORCH study.

BackgroundThe D-TORCH trial demonstrated superiority of 1% topical diclofenac over placebo in preventing capecitabine-induced hand-foot syndrome (HFS). We conducted an exploratory analysis of this study to assess the relationship between HFS and serum levels of the inflammatory marker, cyclooxygenase-2 (COX-2).MethodsSerum COX-2 levels were measured in patients in the D-TORCH study's experimental and placebo arms at baseline and 12 weeks of capecitabine-based therapy or at the development of HFS (whichever occurred earlier) and in 20 age-matched healthy controls using a human COX-2 ELISA kit (

Совпадение в полном тексте источника
… Serum samples were added to the wells and incubated for 90 minutes to facilitate antigen binding, followed by adding a biotinylated detection antibody and avidin-conjugated horseradish peroxidase, with each step accompanied by incubation and several washings. …

Количественное картирование восприимчивости является более чувствительным и специфичным, чем фазовая съемка, при обнаружении хронических активных множественных склерозных покрытий: патологическая проверка.

Количественное картирование и фазовая съемка используются для выявления множественных склерозных повреждений парамагнитными ободами, которые постепенно расширяются с течением времени и связаны с более ранним переходом к инвалидности, снижением объема мозга и увеличением частоты клинического рецидива. Вместе с тем наличие микрогли/макрапажа, погруженных в железо, на повреждённом ободе и обезвоживание в пределах повреждения способствуют получению изображений фазовой и количественной восприимчивости. Таким образом, для оценки точности выявления повреждений, вызванных воздействием железа, требуетс

Совпадение в полном тексте источника
… Sections were further processed for histology and then incubated overnight with a primary antibody against myelin basic protein (MBP, Dako A0623, 1:500), CD68 (Cell Signaling #76437, 1:500) and inducible nitric oxide synthase (iNOS; Novus NB120-15203, 1:300) and a biotinylated secondary antibody and avidin …

The effects of 1 alpha,25-dihydroxyvitamin D(3) on matrix metalloproteinase and prostaglandin E(2) production by cells of the rheumatoid lesion.

INTRODUCTION: 1alpha,25-dihydroxyvitamin D(3)[1alpha,25(OH)(2)D(3)], the biologically active metabolite of vitamin D3, acts through an intra … nce of VDRs by immunolocalisation using a biotinylated antibody and alkaline-phosphatase-conjugated avidin-biotin complex system. Cultured synovial fibroblasts and chrondrocytes were treated with either 1alpha,25(OH)(2)D(3) or interleukin (IL)-1beta, or both. Conditioned medium was assayed for MMP and PGE(2) by enzyme-linked immunosorbent assay (ELISA), …

Radiometals in Imaging and Therapy: Highlighting Two Decades of Research.

The present article highlights the important progress made in the last two decades in the fields of molecular imaging and radionuclide therapy. Advancements in radiometal-based positron emission tomography, single photon emission computerized tomography, and radionuclide therapy are illustrated in terms of their production routes and ease of radiolabeling. Applications in clinical diagnostic and radionuclide therapy are considered, including human studies under clinical trials; their current stages of clinical translations and findings are summarized. Because the metalloid astatine is used for

Совпадение в полном тексте источника
… Based on PRIT technology, [ 90 Y]Y-DOTA-biotin was developed, which makes a strong conjugate with Abs (streptavidin, avidin) present on the tumor. … Paganelli et al. developed an innovative therapeutic approach called “Intra-operative avidination for radionuclide therapy” (IART ® ) that relies on a biotin–avidin binding system [ 241 ]. …

The Current Landscape of Modular CAR T Cells.

Despite the groundbreaking impact of currently approved CAR T-cell therapies, substantial unmet clinical needs remain. This highlights the need for CAR T treatments that are easier to tune, combine, and program with logic rules, in oncology and autoimmunity. Modular CAR T cells use a two-part system: the CAR on the T cell binds an adaptor molecule (AM), and that adaptor binds the tumour-associated antigen (TAA). This design separates recognition of the target antigen and activation of the T cells, resulting in a cellular therapy concept with better control, flexibility, and safety compared to

Совпадение в полном тексте источника
… Urbanska et al. introduced the biotin-binding immune receptor (BBIR) in 2012 [ 21 ], using avidin linked to a second-generation CAR construct. … In 2014, the same group published a different version of their modular CAR that replaced the biotin–avidin with an FRα-FRα scFv, with FRα as the ECD to engage with the adaptor [ 42 ]. …

Hyaluronan (HA) interacting proteins RHAMM and hyaluronidase impact prostate cancer cell behavior and invadopodia formation in 3D HA-based hydrogels.

To study the individual functions of hyaluronan interacting proteins in prostate cancer (PCa) motility through connective tissues, we developed a novel three-dimensional (3D) hyaluronic acid (HA) hydrogel assay that provides a flexible, quantifiable, and physiologically relevant alternative to current methods. Invasion in this system reflects the prevalence of HA in connective tissues and its role in the promotion of cancer cell motility and tissue invasion, making the system ideal to study invasion through bone marrow or other HA-rich connective tissues. The bio-compatible cross-linking proce

Совпадение в полном тексте источника
… Goat-α-rabbit-HRP secondary antibody, protease inhibitors (PI), 5X sample buffer, sulfo-NHS-SS-biotin, and avidin agarose were purchased from Thermo Scientific (Rockford, IL). Laemmli sample buffer and alcian blue were purchased from Biorad (Richmond, CA). … Avidin agarose (1 mL) was applied to a microcentrifuge tube and the resin was settled by centrifuging 1 minute at 2000 RPM. The resin was washed three times with RIPA buffer (500 µl). The lysate was applied to the resin and the mixture was incubated on a tube rotator at 4°C overnight. …

Mesenchymal-Stem-Cell-Based Therapy against Gliomas.

Glioblastoma is the most aggressive, malignant, and lethal brain tumor of the central nervous system. Its poor prognosis lies in its inefficient response to currently available treatments that consist of surgical resection, radiotherapy, and chemotherapy. Recently, the use of mesenchymal stem cells (MSCs) as a possible kind of cell therapy against glioblastoma is gaining great interest due to their immunomodulatory properties, tumor tropism, and differentiation into other cell types. However, MSCs seem to present both antitumor and pro-tumor properties depending on the tissue from which they c

Совпадение в полном тексте источника
… In that study, the MSC cell line C3H10T1/2 was functionalized with the anticancer agent doxorubicin (DOX) encapsulated within liposomes (DOX-Lips) for higher drug loading through the avidin–biotin complex method ( Figure 3 ). … The results were promising, as cell surface modification with DOX-Lips using the avidin–biotin complex method did not affect the proliferation, attachment, migration, or tumor localization ability of C3H10T1/2 cells. Furthermore, it produced a great antitumor effect on Colon26/GFP cells. …