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PmpI antibody reduces the inhibitory effect of Vp1 on Chlamydia trachomatis infectivity.

Chlamydia trachomatis is the most common cause of bacterial sexually transmitted infections. The effect of antibiotic treatment is not satisfactory, and there is currently no vaccine to prevent C. trachomatis infection. Our results showed that Chlamydia virus CPG1 capsid protein Vp1 treatment significantly inhibited C. trachomatis growth in cell culture, and the inclusion numbers of different C. trachomatis serotypes were decreased. In addition, we conducted a preliminary investigation of the possible mechanisms behind the Vp1 inhibition effects and the C. trachomatis molecules targeted by Vp1…

Influence of different delivery modes on the clinical characteristics of Chlamydia trachomatis pneumonia.

UNLABELLED: We analyzed the effects of delivery methods on Chlamydia trachomatis pneumonia in infants. Three hundred forty-four children hospitalized with Chlamydia trachomatis pneumonia were enrolled. They were divided into the vaginal delivery group and the cesarean delivery group. We compared and analyzed their age of onset, peripheral blood white blood cell count, liver enzymes, chlamydia trachomatis titers, and chest radiograph scores. Seventy-eight (22.7%) were delivered by a cesarean, and 266 (77.3%) were delivered vaginally. There were no statistically significant differences between g…

[Document: Chlamydia trachomatis: fundamentals of the importance of screening in the public health system].

The sexually transmitted infection caused by Chlamydia trachomatis has a high prevalence in Chile and other latitudes and is associated with serious risks to the reproductive health of those who become infected. Screening with self- collected samples and detection by amplification of nucleic acids is the most effective form of sanitary control. Until now, in Chile, the diagnosis and epidemiological management of C. trachomatis is not carried out in public health. The Ministry of Health of Chile is making important progress to start this implementation. The Medical Societies are committed to pa…

Врожденный хламидийный трахоматис в среде солдат спецназа Польши.

ВВЕДЕНИЕ И ЦЕЛЬ: Врожденный хламидийный трахоматис является одним из наиболее распространенных бактериальных патогенов, передаваемых половым путем. Цель этого исследования заключалась в том, чтобы представить информацию о нынешних случаях хламидиальных инфекций среди польских солдат, сексуально активных мужчин и женщин репродуктивного возраста. В исследовании приняли участие 253 военнослужащих спецназа Польши, 237 мужчин и 16 женщин в возрасте 26 - 57 лет, находившихся в Варшаве в период с октября по ноябрь 2016 года. Участникам исследования было предложено заполнить социально-демографический

Совпадение в аннотации оригинала
INTRODUCTION AND OBJECTIVE: Urogenital Chlamydia trachomatis is one of the most common sexually transmitted bacterial pathogens. The aim of the study was to present the current occurrence of chlamydial infections among Polish soldiers, sexually-active men and women at reproductive age. MATERIAL AND METHODS: The research involved 253 active duty soldiers fro…

Development of a Proximity Labeling System to Map the Chlamydia trachomatis Inclusion Membrane.

Chlamydia grows within a membrane-bound vacuole termed an inclusion. The cellular processes that support the biogenesis and integrity of thi … alone, or fusion proteins of IncATM (TM = transmembrane domain only), IncA, and IncF with APEX2 in Chlamydia trachomatis serovar L2. IncF-APEX2, IncA TM -APEX2, and IncA-APEX2 localized to the IM whereas APEX2, lacking a secretion signal, remained associated with the bacteria. We determined the impact of overexpression on inclusion diameter, plasmid stability, and Gol …

Use of Group II Intron Technology for Targeted Mutagenesis in Chlamydia trachomatis.

Dissecting the contribution of genes to virulence in fulfillment of Molecular Koch's postulates is essential for developing prevention and treatment strategies for bacterial pathogens. This chapter will discuss the application of a targeted, intron-based insertional mutagenesis method for creating mutants in the obligate, intracellular bacterial pathogen Chlamydia trachomatis. The methods employed for intron targeting, mutant selection, and mutant verification will be outlined including available selection markers, gene targeting strategies, and potential pitfalls.

Comparative study in infertile couples with and without Chlamydia trachomatis genital infection.

BACKGROUND: Chlamydia, caused by the bacterium Chlamydia trachomatis(C. trachomatis), is the most common sexually transmitted disease. The incidence is not clear due to the asymptomatic nature of early stage of infections. The incidence of Chlamydia has not been fully investigated in the Chinese Han population. Since chronic infection with can C. trachomatis can lead to infertility in males and females, it is important to determine the impact of infection on clinical outcomes. The aim of this study is to explore the epidemiology of C. trachomatis in subfertile couples and to determine whether …

[Prevalence and phylogenetic analysis of Chlamydia trachomatis in a population of women in Posadas, Misiones].

OBJECTIVE: Chlamydia trachomatis is the most prevalent bacteria causing sexually transmitted infections. In women, this infection can cause cervicitis and urethritis, although it's usually asymptomatic. The aim of this study was to investigate the prevalence of C. trachomatis in women attending the lab Instituto de Previsión Social and detect the genotypes. METHODS: Endocervical samples from 505 symptomatic and asymptomatic women were assayed. It was determined the presence of C. trachomatis by PCR through amplification of a fragment of the cryptic plasmid. Positive samples were genotyped by …

Routine screening for Chlamydia trachomatis and Neisseria gonorrhoeae in first trimester abortion.

Currently no recommendation exists to collect genital culture for Chlamydia trachomatis and Neisseria gonorrhoeae at diagnosis of spontaneous abortion. A retrospective cross sectional study was performed to identify first trimester abortions with concurrent genital culture collection in an emergency room setting. The results were compared to most current 2015 Center for Disease Control (CDC) statistics. Among women aged 15-24 the rate of C. trachomatis was increased to 20.0% and greater than CDC rate of 6.7% (RR 2.97, p<0.0001). No positive screens for C. trachomatis were found above age 30 an…

Association of human papillomavirus and Chlamydia trachomatis with intraepithelial alterations in cervix samples.

The influence of different infectious agents and their association with human papillomavirus (HPV) in cervical carcinogenesis have not been completely elucidated. This study describes the association between cytological changes in cervical epithelium and the detection of the most relevant aetiological agents of sexually transmitted diseases. Samples collected from 169 patients were evaluated by conventional cytology followed by molecular analysis to detect HPV DNA, Chlamydia trachomatis, herpes simplex virus 1 and 2,Neisseria gonorrhoeae, Mycoplasma genitalium, Trichomonas vaginalis, andTrepon…

Detection of Chlamydia trachomatis and Neisseria gonorrhoeae based on cross-priming amplification.

UNLABELLED: Rapid, sensitive and specific isothermal nucleic acid amplification methods of Chlamydia trachomatis (C. trachomatis) and Neisseria gonorrhoeae (N. gonorrhoeae) have been developed based on cross-priming amplification (CPA). The amplicon of CPA can be detected by a disposable amplicon cross-contamination proof device. The whole assay takes 1-1·5 h from amplification to read out. Ten fold serial dilutions of quantified plasmids were used to test the CPA assay sensitivities, with the detection limits of 45 copies per reaction and 65 copies per reaction for C. trachomatis and N. gonor…

Extragenital Infections Caused by Chlamydia trachomatis and Neisseria gonorrhoeae: A Review of the Literature.

In the United States, sexually transmitted diseases due to Chlamydia trachomatis and Neisseria gonorrhoeae continue to be a major public health burden. Screening of extragenital sites including the oropharynx and rectum is an emerging practice based on recent studies highlighting the prevalence of infection at these sites. We reviewed studies reporting the prevalence of extragenital infections in women, men who have sex with men (MSM), and men who have sex only with women (MSW), including distribution by anatomical site. Among women, prevalence was found to be 0.6-35.8% for rectal gonorrhea (m…

Rectal Infection With Neisseria gonorrhoeae and Chlamydia trachomatis in Men in the United States.

BACKGROUND: Centers for Disease Control and Prevention guidelines recommend at least annual rectal screening of men who have receptive anal intercourse for Neisseria gonorrhoeae (GC) and Chlamydia trachomatis (CT). Only limited national data are available on the prevalence of rectal GC and CT infection among US men. METHODS: In collaboration with a large US commercial laboratory, we estimated positivity of the first rectal GC and CT test ("index" test) in men aged 15-60 years tested between January 2013 and May 2015. We estimated the frequency and positivity of pharyngeal or urine specimens t…

DXD motif-dependent and -independent effects of the chlamydia trachomatis cytotoxin CT166.

The Gram-negative, intracellular bacterium Chlamydia trachomatis causes acute and chronic urogenital tract infection, potentially leading to infertility and ectopic pregnancy. The only partially characterized cytotoxin CT166 of serovar D exhibits a DXD motif, which is important for the enzymatic activity of many bacterial and mammalian type A glycosyltransferases, leading to the hypothesis that CT166 possess glycosyltransferase activity. CT166-expressing HeLa cells exhibit actin reorganization, including cell rounding, which has been attributed to the inhibition of the Rho-GTPases Rac/Cdc42. E…

Background review for the '2015 European guideline on the management of Chlamydia trachomatis infections'.

SummaryChlamydia trachomatis infections are major public health concerns globally. Of particular grave concern is that the majority of persons with anogenital Chlamydia trachomatis infections are asymptomatic and accordingly not aware of their infection, and this silent infection can subsequently result in severe reproductive tract complications and sequelae. The current review paper provides all background, evidence base and discussions for the 2015 European guideline on the management of Chlamydia trachomatis infections (Lanjouw E, et al. Int J STD AIDS 2015). Comprehensive information and r…

Roles of bovine Waddlia chondrophila and Chlamydia trachomatis in human preterm birth.

Waddlia chondrophila and Chlamydia trachomatis are intracellular bacteria associated with human miscarriage. We investigated their role in human preterm birth. Whereas presence of Chlamydia trachomatis DNA in genital tract was associated with human preterm birth, Waddlia was not, despite being present in women's genital tracts.

[Risk behaviours and Chlamydia trachomatis prevalence in prisoners by length of stay in prison].

BACKGROUND AND OBJECTIVE: Young prisoners have high-risk behaviors and socio-economic insecurity that increases vulnerability for Chlamydia trachomatis (CT) acquisition. Monitoring its prevalence will help to reduce infection rates. PATIENTS AND METHODS: Cross-sectional study from a convenience sample of prisoners aged 18-25 years. Urine samples were obtained to determine CT. A standardized and anonymous questionnaire was used to collect the study variables. RESULTS: The overall CT prevalence was 11%, significantly higher in those with less than one year in prison, who also presented higher …

Chlamydia trachomatis epidemiology in Canadians of Chinese ethnicity: a population-based study.

OBJECTIVES: To compare the rates of Chlamydia trachomatis infection between those of Chinese ethnicity and non-Chinese ethnicity in a large Canadian urban setting. METHODS: We examined rates of Chlamydia among residents of Vancouver and Richmond, Canada, by Chinese and non-Chinese ethnicity, from 2006 to 2010. We stratified cases by age group, sex and ethnicity. We analyzed 12,555 cases of Chlamydia from 2006 to 2010. RESULTS: The overall rate of Chlamydia was 276 per 100,000 per year. Chlamydia rates were 236 per 100,000 among those of Chinese ethnicity and 338 per 100,000 among non-Chinese…

Chlamydia trachomatis and Genital Mycoplasmas: Pathogens with an Impact on Human Reproductive Health.

The most prevalent, curable sexually important diseases are those caused by Chlamydia trachomatis (C. trachomatis) and genital mycoplasmas. An important characteristic of these infections is their ability to cause long-term sequels in upper genital tract, thus potentially affecting the reproductive health in both sexes. Pelvic inflammatory disease (PID), tubal factor infertility (TFI), and ectopic pregnancy (EP) are well documented complications of C. trachomatis infection in women. The role of genital mycoplasmas in development of PID, TFI, and EP requires further evaluation, but growing evid…