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Glycosylation and secretion of yellow fever virus nonstructural protein NS1.

The flavivirus genome codes for structural and nonstructural proteins which are produced from a common polyprotein precursor by proteolytic processing. The nonstructural protein NS1 is hypothesized to be involved in virus assembly and maturation as well as in protective immunity. This paper describes the synthesis of several forms of yellow fever (YF) virus NS1 protein during virus cultivation in vitro. These include cell-associated and secreted NS1 forms, the major difference among these being their N-linked glycosylation status. High-mannose and complex (or hybrid)-type moieties were identif…

Effect of human gamma interferon on yellow fever virus infection.

We studied yellow fever virus infection in two species of monkey: Saimiri sciureus (squirrel monkeys) and Macaca mulatta (rhesus monkeys). Human gamma interferon was administered intravenously in five equal doses, one was given 24 hr before infection followed by four doses 24 hr apart. Interferon reduced the levels and duration of viremia and the severity of hepatitis in squirrel monkeys. Interferon prolonged survival time and delayed the appearance of viremia and hepatitis in infected rhesus monkeys, but it did not change overall mortality.

Трансовариальная передача вируса желтой лихорадки комарами (Aedes aegypti).

Женские комары Aedes aegypti, инфицированные вирусом желтой лихорадки внутри грудной клетки, передали вирус небольшой процент своего потомства F1. Зараженные потомки были получены из стерилизованных на поверхности, а также из неочищенных яиц, что указывает на трансовариальную передачу вируса. Вертикальная передача вируса желтой лихорадки у комаров может быть альтернативным механизмом биологического выживания вируса в неблагоприятных периодах или при отсутствии восприимчивых носителей позвоночных.

Совпадение в аннотации оригинала
Female Aedes aegypti mosquitoes infected with yellow fever virus by intrathoracic inoculation transmitted the virus to a small percentage of their F1 progeny. Infected offspring were obtained from surface-sterilized as well as from untreated eggs, indicating that the virus was transovarially transmitted. Vertical transmission of yellow fever virus in mosquit…

Yellow fever virus. II. Factors affecting the plaque neutralization test.

In studies on the factors and conditions influencing the yellow fever (YF) virus plaque neutralization test, 60 min was found to be the minimal time necessary for equilibration of the virus-antibody complex at 23 and 37 C. Maximal virus titers in the diluent controls and the pre- and postinoculation serum-containing mixtures occurred by the 60-min adsorption time. Serum neutralization indices also seemed to level by this time. Heating (56 C for 30 min) decreased the neutralizing capacity of serum. However, the slope of the neutralization curve was not affected. The addition of native (unheated…

Lethal 17D yellow fever encephalitis in mice. I. Passive protection by monoclonal antibodies to the envelope proteins of 17D yellow fever and dengue 2 viruses.

Monoclonal antibodies to the envelope proteins (E) of the 17D vaccine strain of yellow fever virus (17D YF) and to dengue 2 virus were examined for their ability to confer passive protection against lethal 17D YF encephalitis in mice. All 13 IgG anti-17D YF antibodies, regardless of neutralizing capacity, conferred solid protection when given in a relatively high dose prior to intracerebral inoculation of virus. Three antibodies with high in vitro neutralizing titres were all protective at a low dose as were several non-neutralizing antibodies. One flavivirus group-reactive antibody to dengue …

Integrated surveys of Rift Valley fever, yellow fever and dengue in Lobaye, Central African Republic.

BACKGROUND: Confirmation by plaque reduction neutralization testing (PRNT) of concurrent cases of Rift Valley fever virus (RVFV), yellow fever virus (YFV) and dengue virus (DENV) in Safa village (Lobaye, Central African Republic, CAR) triggered a One Health investigation to assess human, animal, entomological and socio-environmental risk factors for arboviral transmission. METHODS: We conducted integrated epidemiological surveys, blood sampling in humans and domestic animals, larval and adult mosquito collections, and socio-anthropological interviews in Safa and the neighbouring village of Po…