Результаты поиска

Antigenic properties of Chlamydia trachomatis lipopolysaccharide.

The antigenic properties of the lipopolysaccharide (LPS) of Chlamydia trachomatis L2 were investigated. By means of passive hemolysis, passive hemolysis inhibition, and absorption experiments, it was shown that antiserum raised against chlamydial elementary bodies contained at least two different antibody specificities which reacted with different antigenic determinants of chlamydial LPS. One of these antibodies cross-reacted with enterobacterial Re LPS, recognizing a structure which is shared by both LPSs, whereas the reactivity of the second antibody was restricted to chlamydial LPS. The for…

Chronic prostatitis, chlamydia trachomatis and infertility.

Seventy-one infertile men were studied for the presence of seminal leucocytosis, and for the levels of acid phosphatase activity and Chlamydia trachomatis antibodies of IgA class using a novel method of solid-phase radioimmunoassay. The findings were compared with those of 56 fertile men. Chlamydial IgA antibodies were found in the semen of 51.1% of infertile men with seminal leucocytosis and decreased seminal acid phosphatase activity, i.e. those defined as having asymptomatic chronic prostatitis. This frequency was significantly higher than among fertile men (23.2%, P less than 0.01) and tho…

Therapeutic abortion and Chlamydia trachomatis infection.

Chlamydia trachomatis was isolated from the cervix of 30 of 218 (13.8%) women admitted for legal termination of pregnancy. During the first two weeks after the abortion seven of the 30 (23.3%) patients developed pelvic inflammatory disease. Four of these had serological evidence of recent active chlamydial infection. Thus, routine examination of patients for genital chlamydial infection before termination of pregnancy is recommended.

Microbiology Diagnosis of chlamydia trachomatis infection.

The etiological diagnosis of what is today known as infection by Chlamydia trachomatis was first made possible in 1907 when Halberstaedter and von Prowazek identified inclusions in conjunctival scrapings by means of Giemsa staining. C. trachomatis was originally classified as a virus, and the culture systems used were those for viruses. Macchiavello was the first to describe the isolation of C. trachomatis in embryonated hens' eggs (1944), but the first isolation is usually credited to T'ang and co-workers (1957), also using eggs. A major step in the understanding of chlamydial infections was …

Infertility and cervical Chlamydia trachomatis infections.

Of the 51 women examined for infertility, 19.6 per cent were found Chl. trachomatis culture-positive. This differs, although not significantly, from the 9 per cent isolation rate among our general gynecological outpatients. The results suggest that Chl. trachomatis should be considered in women with unexplained infertility.

Chlamydia trachomatis cervicitis in gynecologic outpatients.

Symptoms suggestive of a lower genital tract infection (LGTI) are common complaints in women who consult gynecologists. Sexually transmitted microorganisms, such as Chlamydia trachomatis. Neisseria gonorrhoeae, and Trichomonas vaginalis, are responsible for a substantial proportion of LGTI. This study was performed to establish the frequency of LGTI caused by C trachomatis in women attending a gynecologic outpatient clinic. Of 170 women with LGTI, 32.9% harbored one or more of these organisms: C trachomatis was found in 19.3%, N gonorrhoeae in 4.7%, and T vaginalis in 25.9%. The results of the…

Представление хламидийского трахоматиса OmcB Enzime-Linked Immunosorbent Assay в серодиагностике хламидиотрахоматиза инфекции у женщин.

Для оценки бремени заражения хламидией trachomatis и эффективности профилактических усилий необходимы серологические оценки хламидии trachomatis с повышенным уровнем чувствительности по сравнению с коммерческими оценками. Мы провели оценку эффективности внешнего мембранного белка B (OmcB) энзимно привязанного иммуносорбента (ELISA) в обнаружении антиC. trachomatis антителной реакции у женщин, инфицированных трахоматисом. AmcB ELISA был менее чувствительным, чем наше элементарное тело C. trachomatis (EB) ELISA, но он был очень специфичен. Масштабы реакции антител были выше у афроамериканцев и у

Совпадение в аннотации оригинала
Chlamydia trachomatis serological assays with improved sensitivity over commercially available assays are needed to evaluate the burden of C. trachomatis infection and the effectiveness of prevention efforts. We evaluated the performance of a C. trachomatis outer membrane complex protein B (OmcB) enzyme-linked immunosorbent assay (ELISA) in the detection of …

Use of a Chlamydia trachomatis DNA probe for detection of ocular chlamydiae.

We examined the efficacy of a Chlamydia trachomatis DNA probe in detecting ocular chlamydiae by comparing it with tissue culture isolation, direct fluorescent-antibody cytology, and clinical eye exams. In a trachoma-endemic area of Nepal, 430 Nepalese villagers were examined according to the World Health Organization trachoma grading scale. Upper tarsal conjunctival specimens from each subject were obtained for DNA probing, tissue culture, and fluorescent-antibody screening. Moderate to severe intensity of inflammation was found in 85 (21%) of 430 people studied. An additional 25 (7.2%) of 345…

Sphingolipid Metabolism and Transport in Chlamydia trachomatis and Chlamydia psittaci Infections.

Chlamydia species infect a large range of vertebral hosts and have become of major economic and public health concern over the last decades. … hlamydia species acquire sphingomyelin from the host cell. In recent years a clearer picture of how Chlamydia trachomatis acquires this lipid emerged showing that the bacteria interact with vesicular and non-vesicular transport pathways that involve the recruitment of specific RAB proteins and the lipid-transfer protein CERT. These interactions contribute to the develo …

Advances in genetic manipulation of Chlamydia trachomatis.

Chlamydia trachomatis, one species of Chlamydia spp., has the greatest impact on human health and is the main cause of bacterial sexually transmitted diseases and preventable blindness among all Chamydia spp. species. The obligate intracellular parasitism and unique biphasic developmental cycle of C. trachomatis are the main barriers for the development of tools of genetic manipulation. The past decade has witnessed significant gains in genetic manipulation of C. trachomatis, including chemical mutagenesis, group II intron-based targeted gene knockout, fluorescence-reported allelic exchange mu…

The role of tryptophan in Chlamydia trachomatis persistence.

Chlamydia trachomatis (C. trachomatis) is the most common etiological agent of bacterial sexually transmitted infections (STIs) and a worldwide public health issue. The natural course with C. trachomatis infection varies widely between individuals. Some infections clear spontaneously, others can last for several months or some individuals can become reinfected, leading to severe pathological damage. Importantly, the underlying mechanisms of C. trachomatis infection are not fully understood. C. trachomatis has the ability to adapt to immune response and persist within host epithelial cells. Ind…

Impact of antiseptics on Chlamydia trachomatis growth.

UNLABELLED: Bacterial vaginosis is a frequent dysbiosis, where the normal lactobacillus-dominated flora is replaced by an anaerob/aerob polymicrobial flora. Bacterial vaginosis increases the risk of acquiring sexually transmitted infections (STI) including the most frequent Chlamydia trachomatis infections. Intravaginal antiseptics are part of the bacterial vaginosis treatment, and ideally they should also inhibit the bacterial vaginosis-related STI. Therefore, we tested the antichlamydial activity of four antiseptics: iodine aqueous solution, povidone-iodine, chlorhexidine and borax. First, w…

Diagnosis and treatment of Chlamydia trachomatis infection.

Chlamydia trachomatis infection most commonly affects the urogenital tract. In men, the infection usually is symptomatic, with dysuria and a discharge from the penis. Untreated chlamydial infection in men can spread to the epididymis. Most women with chlamydial infection have minimal or no symptoms, but some develop pelvic inflammatory disease. Chlamydial infection in newborns can cause ophthalmia neonatorum. Chlamydial pneumonia can occur at one to three months of age, manifesting as a protracted onset of staccato cough, usually without wheezing or fever. Treatment options for uncomplicated u…

[The infections by Chlamydia trachomatis and Neisseria gonorrhoeae].

Among sexually transmitted diseases, Chlamydia trachomatis and Neisseria gonorrhoeae infections are found most frequently. Recently, their incidence in youths has increased. The rate of infection by human immunodeficiency virus is increased in patients who have been infected by Chlamydia trachomatis. In view of this, early detection and treatment of Chlamydia trachomatis infection appear to be important. Asymptomatic infections by Chlamydia trachomatis and Neisseria gonorrhoeae are not rare among gynecological patients. However, in patients infected by both of these pathogens it is difficult t…