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Presence-absence sequential sampling plan for northern fowl mite, Ornithonyssus sylviarum (Acari: Macronyssidae), on caged-layer hens.

Caged-layer hens were scored as infested or uninfested by visual examination of the vent region, and the number of northern fowl mite, Ornithonyssus sylviarum (Canestrini & Fanzago), per hen was estimated. The proportion infested and average number of mites per hen were shown to have a highly significant, positive relationship (r = 0.936). Sampling among houses within a flock, and rows and sections within houses were analyzed to determine the reliability of sampling a representative portion of a flock. Low- and moderate-tolerance treatment thresholds, based on percentage of hens infested with …

Cross-reactivity between terrestrial snails (Helix species) and house-dust mite (Dermatophagoides pteronyssinus). I. In vivo study.

Clinical reports have suggested an unusual frequency in the number of patients with food allergy to snails who are also allergic to the house-dust mite (HDM). As allergy to HDM is one of the most frequent sensitizations in atopic patients of Western countries, evaluation of the relevance of the concomitant sensitization to Dermatophagoides pteronyssinus and to snails is an important consideration. To evaluate the responsibility of different snail components and of snail mites for inducing in vivo hypersensitivity in patients allergic to HDM, the in vivo reactivity of patients with clinical sym…

Evaluation of household dust mite exposure and levels of specific IgE and IgG antibodies in asthmatic patients enrolled in a trial of immunotherapy.

BACKGROUND: Monitoring the response to immunotherapy entails understanding exposure to relevant allergens. For the major indoor allergens, this requires sampling of dust from the patient's house. The objectives of this study were to measure indoor allergen levels during a controlled trial of dust mite immunotherapy for asthma and to relate these results to serum antibody levels. METHODS: Eighty-eight asthmatic patients with mite allergy from seven geographic areas in the United States were enrolled in and completed a course of immunotherapy with Dermatophagoides extract or placebo control. Se

Митохондриальная ДНК из паучьего клеща: изоляция, карта ограничений и частичная последовательность гена цитохромного оксидаза I.

Была очищена митохондриальная (mt) ДНК фитофагиозного клеща Tetranychus urticae и была построена карта ограничений. Длинный геном 12,5 кБ — самое короткое животное, известное MtDNA. 564 bp клон, являющийся частью гена цитохромного оксидаза, я был секвенирован. Как было обнаружено у насекомых, митохондриальные последовательности клещей чрезвычайно богаты A+T (75% в среднем, 96,5% в третьем кодонном положении).

Совпадение в аннотации оригинала
Mitochondrial (mt) DNA of the phytophagous mite Tetranychus urticae was purified and a restriction map was constructed. The 12.5 kb long genome is the shortest animal mtDNA known. A 564 bp clone comprising part of the gene for cytochrome oxidase subunit I was sequenced. As has been found in insects, the mitochondrial sequences of mites are extremely A+T rich…

Non-consumptive effects of a predatory mite volatile: Dimethyl trisulfide disrupts mating, oviposition, and ovarian development in Phthorimaea operculella.

BACKGROUND: Predator-derived chemical cues suppress pest reproduction through non-consumptive pathways and may be exploitable as semiochemicals in integrated pest management. We evaluated the volatile organic compounds (VOCs) emitted by the predatory mite Pyemotes zhonghuajia, for their inhibitory effects on reproduction in the potato tuber moth (Phthorimaea operculella, PTM). RESULTS: Screening of 34 mite-associated VOCs identified dimethyl trisulfide (DMTS) as the strongest oviposition suppressor. In dose-response assays, DMTS reduced egg-laying (>80% at 0.9 mg mL-1), shortened the oviposit

Estimation of economic injury levels and action thresholds for the two-spotted spider mite, Tetranychus urticae Koch, on strawberry plants.

Strawberry (Fragaria × ananassa Duch.) is a high-value global crop, yet its productivity is severely constrained by the two-spotted spider m … *** in 2020), with an average yield reduction of 1.25% per mite. The estimated EIL and ETL were 4.0 mites/leaf in 2019 and 3.4 mites/leaf in 2020. These findings provide a precise decision-making framework for mite management in Mediterranean strawberry production. The low estimated thresholds suggest that proactive, preventive management strategies are …

Combined use of Beauveria bassiana and the predatory mite Neoseiulus californicus to suppress Tetranychus urticae on greenhouse-grown sweet pepper.

Predatory mites and invertebrate-pathogenic fungi are important biological control tools for managing populations of the two-spotted spider mite, Tetranychus urticae, in horticultural field crops. Here, we evaluated strategies for the combined use of a mite-pathogenic strain of Beauveria bassiana and Neoseiulus californicus to suppress T. urticae populations on sweet pepper plants. First, we compared the performance of both biocontrol agents, applied alone or in combination, under greenhouse conditions. A decline in T. urticae populations was observed 7 days after the release of the predatory …

Butyrate attenuates house dust mite-induced activation of 16HBE bronchial epithelial cells in association with modulation of gene expression.

INTRODUCTION: The airway epithelial barrier is the primary defense line of the lungs against allergens, such as house dust mite (HDM). A disrupted airway epithelial barrier plays a major role in asthma development. Short-chain fatty acids (SCFAs) are bacterial fermentation products that are associated with prevention against allergic inflammation. In this study, we aim to elucidate the direct preventative effects of SCFAs, particularly butyrate, on the HDM-induced bronchial epithelial cell (BEC) activation and barrier disruption in-vitro. MATERIALS AND METHODS: Immortalized human 16HBE cells

Water mite larvae (Hydrachnidia, Arrenurus) parasitism of Anopheles albimanus and An. vestitipennis in a Mexican Neotropical region, including parasite descriptions.

Parasitic interactions between water mite larvae (Acari: Hydrachnidia) and mosquitoes (Diptera: Culicidae) remain poorly understood, especia … (SEM) and DNA barcoding, leveraging previously constructed DNA libraries from the region. The water mites were identified as Arrenurus sp. and Arrenurus federicoi. This study represents the first record of these mosquito species being parasitized by water mites in the Neotropical region. Furthermore, it is the first to employ a multifaceted approach, in …

Effects of MSC-derived extracellular vesicles and dupilumab on the spleen and skin in a house dust mite-induced atopic dermatitis model.

Atopic dermatitis (AD) is a chronic inflammatory skin disease characterized as a biphasic T-lymphocyte-mediated disease involving T-helper cells. Dupilumab is a human monoclonal antibody that inhibits Th2-related cytokines and has recently been approved for patients with AD. However, the effects of dupilumab are relatively narrow in scope, primarily targeting cytokine-driven inflammatory pathways, with few reported systemic effects. Mesenchymal stem cell-derived extracellular vesicles (MSC-EVs) could serve as an alternative therapeutic strategy for AD. This study aims to compare the effects of

House Dust Mite Nebulization Drives Alarmin and Complement Activation in a Murine Tracheal Air-Liquid Interface Culture System.

Air-liquid interface (ALI) cultures offer a physiologically relevant in vitro model of the airway epithelium (AE), capable of recapitulating key structural and functional features observed in vivo. In this study, we established and validated a murine ALI culture system comprising pseudostratified epithelia with functional tight junctions, ciliated cells and goblet cells. To assess their innate immune functions, we designed and 3D-printed an autoclavable aerosol deposition chamber, which allowed us to expose differentiated AE cultures to house dust mite (HDM) allergen. Upon HDM exposure, AE cel

Three new species of the feather mite genus Trouessartia (Acariformes: Trouessartiidae) from passerines (Passeriformes) in the Asian part of Russia.

Three new species of the feather mite genus Trouessartia are described from passerines from various regions in the Asian part of Russia: Trouessartia locustellae sp. nov. from the Pallass grasshopper warbler Helopsaltes certhiola (Pallas) (type host) and the Middendorffs grasshopper warbler H. ochotensis (Middendorff) (Locustellidae), T. mugimaki sp. nov. from the mugimaki flycatcher Ficedula mugimaki (Temminck) (Muscicapidae), and T. pyrrhulae sp. nov. from the Eurasian bullfinch Pyrrhula pyrrhula Linnaeus (Fringillidae). Descriptions and differential diagnoses are supplemented with sequences

Sleeping with the enemy II: Expanding the ecological, molecular, and epidemiological knowledge of the tropical fowl mite, Ornithonyssus bursa (Berlese, 1888).

Ornithonyssus bursa (Berlese), the tropical fowl mite from the family Macronyssidae, is a hematophagous ectoparasite of birds with increasing importance in human and animal health. This study reports new cases of human parasitism associated with O. bursa in Brazil, involving direct contact with avian hosts or their nests. These cases include new geographic records in the states of São Paulo and Santa Catarina, and new associations with bird species, including the first known record in Amazona aestiva (Psittaciformes). Molecular analysis was performed on individual mites to characterize the spe…

Biocontrol agents and their potential use as nano biopesticides to control the tea red spider mite (Oligonychus coffeae): A comprehensive review.

Tea red spider mite (TRSM), Oligonychus coffeae Nietner, is one of the major pests that cause considerable crop losses in all tea-growing co … t beneficial insects. Besides, some predators, including green lacewings, ladybirds, and phytoseiid mites have the potential to control TRSM. Employing these biocontrol agents simultaneously in tea plantations could be more effective in preventing TRSM. Nevertheless, their high biodegradability rate, uneven distribution, and uncontrolled release pose ch …

Cold storage as part of a Varroa management strategy: effects on honey bee colony performance, mite levels and stress biomarkers.

Placing honey bee colonies in cold storage has been proposed as a way to induce a pause in brood production as part of a Varroa mite treatment plan. Here, we exposed colonies to combinations of with or without an October cold storage period and with or without a subsequent miticide application. We then measured the effects of those treatments on colony-level variables (i.e. colony size, Varroa infestation level, survivorship and hive weight and temperature) and pooled individual-level variables that are associated with nutritional and stress responses. Colonies were assessed before and after c

The structural analysis of secretion in the freshwater mite Limnesia maculata (Acariformes, Limnesiidae) supports the idea of a new form of arthropod silk.

The structural characteristics of silk secretion of the freshwater mite Limnesia maculata (O.F. Müller) (Acariformes, Limnesiidae) are descr … contrary, are quite thin with through foramens. The revealed organization of silk in the freshwater mites is found to be the simplest among that of other arthropods. We propose a role of the silk in the capture of potential prey in the summer season. Silk in water mites significantly widens the wholesome area for the mites' life and gives them better ch …

Characterization of sex-related differences in allergen house dust mite-challenged airway inflammation, in two different strains of mice.

Biological sex impacts disease prevalence, severity and response to therapy in asthma, however preclinical studies often use only one sex in murine models. Here, we detail sex-related differences in immune responses using a house dust mite (HDM)-challenge model of acute airway inflammation, in adult mice of two different strains (BALB/c and C57BL/6NJ). Female and male mice were challenged (intranasally) with HDM extract (~ 25 μg) for 2 weeks (N = 10 per group). Increase in serum HDM-specific IgE showed a female bias, which was statistically significant in BALB/c mice. We compared naïve and HDM

House dust mite sensitization and exposure affects bronchial epithelial anti-microbial response to viral stimuli in patients with asthma.

INTRODUCTION: Allergen exposure worsens viral-triggered asthma exacerbations and could predispose the host to secondary bacterial infections. We have previously demonstrated that exposure to house dust mite (HDM) reduced TLR-3-induced IFN-β in human bronchial epithelial cells (HBECs) from healthy donors. We hypothesize that HDM sensitization in different ways may be involved in both viral and bacterial resistance of HBECs in asthma. In this study, the role of HDM sensitization and effects of HDM exposure on viral stimulus-challenged HBECs from asthmatic donors have been explored with regard to

Papulopustular Rosacea Treated With Ivermectin 1% Cream: Remission of the Demodex Mite Infestation Over Time and Evaluation of Clinical Relapses.

INTRODUCTION: Topical ivermectin is an anti-inflammatory and anti-Demodex drug for papulopustular rosacea. Rosacea is a relapsing disease and the time between recurrences should be considered alongside efficacy. OBJECTIVES: The aims of this study were to assess the time of first relapse and relapse rates of Demodex mite infestation and papulopustular rosacea. METHODS: We conducted a prospective study of subjects affected by different degrees of papulopustular rosacea. Patients that achieved a complete response after treatment were monitored every 4 weeks and up to 32 additional weeks. For ea