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Structure of the collagen nodule from hypertrophic scars and keloids.

The collagen nodule is the structural unit of all hypertrophic scars in keloids. It is never found in the fully mature scars. A study of the fine structure of the nodule might reflect its origin, and, hence, origin of the hypertrophic scar and keloid. Nodules are composed of a marked increase of unidirectional collagen fibrils aligned in a highly stressed orientation. There is also a marked increase in the number of fibroblasts. Few microvessels appear within the nodule; rather, they remain peripheral and encompass the main body of the nodule as a net. There is a heavier concentration of micro…

The ultrastructural characteristics of abnormal collagen fibrils in various organs.

Quite unrelated diseases affecting different organs can lead to the appearance of abnormal collagen fibrils, which may be due to genetic disorders, transformation of mesenchyme cells, increased collagen synthesis or a change in the ground-substance. The simultaneous appearance of abnormal collagen fibrils and matrix vesicles containing lysosomal enzymes in the majority of our electron photomicrographs suggests an alteration in the ground-substance as a main cause of non-hereditary collagen dysplasia.

Immunochemical study on basement membrane (type IV) collagens.

Basement membrane (type IV) collagens were extracted from a mouse tumour with acetic acid and from human placenta after limited enzymatic digestion. Antisera were produced against both collagens in rabbits and guinea-pigs and examined by various assays. These antisera were found to be specific for basement membrane collagen and showed little or no cross-reactions with the interstitial collagens, types I, II and III or with human placenta collagen consisting of alpha A and alpha B chains. Varying degrees of cross-reaction were observed between antisera to human and mouse type IV collagen. Immun…

Collagenous bone matrix-induced endochondral ossification hemopoiesis.

Transplantation of collagenous matrix from the rat diaphyseal bone to subcutaneous sites resulted in new bone formation by an endochondral sequence. Functional bone marrow develops within the newly formed ossicle. On day 1, the implanted matrix was a discrete conglomerate with fibrin clot and polymorphonuclear leukocytes. By day 3, the leukocytes disappeared, and this event was followed by migration and close apposition of fibroblast cell surface to the collagenous matrix. This initial matrix-membrane interaction culminated in differentiation of fibroblasts to chondroblasts and osteoblasts. Th…

Posttranslational protein modifications, with special attention to collagen and elastin.

It is apparent that significant progress has been made in our understanding of the biosynthesis, modifications, and maturation of collagen and elastin. We now recognize and partially understand special reactions involved in hydroxylations within the cell and complex cross-linking processes occurring outside the cell. Recent experiments (191) have shown that in human diploid fibroblast cultures of limited doubling potential (191) the hydroxylation of collagen prolyl residues appears to be "age" or passage-level dependent. With increasing passage level of these cultures, both the ascorbate requi…

Follistatin Mitigates Myofibroblast Discrimination и Collagen System of Fibroblast из Scar Tise вокруг поврежденного Flexor Tendons.

ЦЕЛЬ: Цель этого исследования состояла в изучении воздействия гена FST на ингибирование фиброза в фиброблатических клетках от шрамных тканей вокруг отремонтированных сухожилий зоны II. МАТЕРИАЛЫ И МЕТОДЫ: Иммуногистохимия была проведена на фибробалластных клетках, передающихся с помощью аденовируса-Лакза (Ад-Лакза) в качестве маркерного гена (контроля), или с аденовирусом-ФСТ (АД-ФСТ) в качестве терапевтического гена. Культуры фибробласта без аденовального воздействия служат средством контроля. Результаты: Фибробластические клетки, передающиеся с помощью AD-FST, продемонстрировали значительное

Совпадение в аннотации оригинала
PURPOSE: The aim of this study was to investigate the effect of FST gene on the inhibition of fibrosis in fibroblastic cells from scar tissu … controls. RESULTS: Fibroblastic cells transfected with Ad-FST demonstrated significant decrease in collagen type I, MMP-1, MMP2, and α-SMA mRNA expressions compared to those transfected with Ad-LacZ. In addition, fibroblastic cells transfected with Ad-FST exhibited significant decrease in MMP-1, TIMP-1, fibronectin, PAI-1, TRPV4, α-SMA, desmin, and PAX7 p …

Retrospective study on the outcomes of vestibuloplasty with a xenogeneic collagen matrix.

PURPOSE: To retrospectively evaluate the effects of vestibuloplasty using a xenogeneic collagen matrix (XCM) at implant sites with insufficient peri-implant keratinized tissue (PIKT) and a shallow peri-implant vestibule (PIV). METHODS: Patients who underwent vestibuloplasty with an XCM between July 2021 and August 2023 at Kyung Hee University Dental Hospital were included. Inclusion criteria were PIKT <2 mm, PIV <4 mm at baseline, and a follow-up duration of >6 months. Clinical parameters, including PIKT and PIV, were measured at baseline and at 1 month, 6 months, and the final visit. Margina…

Targeting Circadian Rhythm for the Regulation of Skin Collagen Metabolism.

BACKGROUND: Collagen is essential for maintaining skin structure and function, and the circadian rhythm is known to regulate a wide range of physiological processes. AIMS: To investigate whether collagen metabolism in human skin fibroblasts exhibits circadian regulation, and to evaluate whether the time-coordinated application of baicalin at daytime and palmitoyl tripeptide-1 (PT-1) at nighttime synergistically promotes collagen fiber formation and improves overall skin quality. METHODS: A circadian-synchronized human skin fibroblast model was established. The expression of collagen metaboli…

Expression of Recombinant Collagen Domains and Matrikines in Eukaryotic Cells.

The expression of extracellular matrix protein domains, which exhibit biological activities of their own, is required in order to get a sufficient amount to perform experiments aiming at determining their structure, interactions, and functions, and for preclinical and clinical studies. We describe here the protocol we designed to express in human embryonic kidney cells a recombinant von Willebrand factor A domain of human collagen VI, and recombinant anti-angiogenic matrikines of human collagens VIII (vastatin) and XVIII (endostatin).

Rare Concurrent Findings With Collagenous Gastroduodenitis in a Pediatric Patient.

Collagenous gastritis is a rare type of gastritis characterized by thickened subepithelial collagen and chronic inflammatory infiltrate. We describe the unique presentation of a previously healthy 13-year-old girl, who presented with severe fatigue and exertional dyspnea. The gastric and duodenal histologic features were consistent with collagenous sprue, collagenous gastritis, and chronic atrophic gastritis. To the best of our knowledge, the simultaneous presentation of these entities is highly uncommon in children, underscoring the importance of understanding its pathogenesis.

Characterization of collagen profile in peritoneal metastases of colorectal cancer.

The tumor microenvironment (TME) plays a critical role in cancer progression and response to treatment. In colorectal cancer (CRC), a collagen-rich extracellular matrix (ECM) is a well-established feature of primary tumors and liver metastases. However, the composition of the ECM in peritoneal metastases remains poorly characterized. In this descriptive study, we analyzed the histological distribution of four major collagen types (I, II, III, and IV) in peritoneal metastases from 39 CRC patients using immunohistochemical techniques. Type III fibrillar collagen was predominant, showing moderate…