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DRG meningeal tertiary lymphoid structures are regulated by B cells as a pronociceptive locus after peripheral nerve injury.

B cell-derived IgG in the dorsal root ganglia (DRG) drives neuropathic pain after peripheral nerve injury (PNI), but the site of B cell organization is unclear. Here, PNI induced leukocyte clusters in the DRG meninges, enveloped by lymphatic endothelium and apposed to high endothelial venules. These clusters resemble tertiary lymphoid structures (TLSs) with germinal center-like features, including germinal center B cells and plasma cells, and follicular dendritic and follicular helper T cells. Single-cell RNA sequencing revealed enrichment of germinal center B cells in the DRG meninges after P…

Wasteosomes accumulate in periventricular white matter hyperintensities and exhibit complex relationships with mixed pathology, sclerotic index, and perivascular space.

Corpora amylacea or "wasteosomes" are discontinuous lipid labyrinth structures that are polyglucosan rich, retain cellular waste and are tho … d PVS. Our results reveal wasteosomes accumulate in PV WMHs, are increased in proximity to large PV venules, and exhibit complex relationships with WMH severity, mixed pathology, sclerotic index and PVS. These findings provide novel insights into the pathophysiology underlying white matter injury.

Standardized Instance-Level Quantification of CD34-Positive Vessels in Lymph Node Whole-Slide Images Using U-Net.

Deep learning enhances quantitative analysis of blood vessels in whole-slide images (WSIs), leading to precise and reliable counting. However, counting vessels in lymph nodes-which contain distinctive high endothelial venules (HEVs)-has not been systematically evaluated. Artificial intelligence (AI) approaches to vessel-counting fall into two categories: semantic segmentation, which provides a continuous mask, and instance segmentation, which treats each vessel as a separate object; prior work has emphasized the former. To address this gap, we adopted an instance-level approach to detect and c…

High Resolution Optical Microscopy Technique Aplicated to Study of Placenta of Mummificated Foetuses in Swine.

In the sows, fetal mortality that occurs between Day 35 and term, causes on average the loss of more than one piglet per litter. During pren … ze, arrangement and morphological characteristics. Numerous capillaries arranged around areolas and venules were observed. Microthrombus formation was not observed. Cytoplasmic vacuolization, chromatolysis and cell detachment were observed in the trophoblastic epithelium. These all characteristics correspond to postmortem morphological changes. The HRLM a …

Sucnr1 Mediates Leukocyte-Endothelial Cell Interactions Induced by Tnfα.

Succinate is a metabolite involved in chronic inflammatory diseases, regulating macrophages, dendritic cells, and lymphocytes via its receptor SUCNR1 and through intracellular pathways. Our aim was to analyze whether the succinate-SUCNR1 axis modulates the leukocyte-endothelium interactions (L/EI) that mediate the formation of inflammatory foci in response to a ubiquitous pro-inflammatory cytokine (TNFα). L/EI were analyzed in murine cremasteric venules in vivo and between human umbilical endothelial cells (HUVECs) and peripheral blood mononuclear cells (PBMCs) in vitro. We observed that TNFα …

Epididymitis promotes formation of tertiary lymphoid organs in the cauda epididymidis.

Uropathogenic Escherichia coli is a common pathogen that affects the cauda epididymidis, causing interstitial edema, epithelial damage, leuk … ost-infection. These appear as compartmentalized B- and T-cell clusters containing high endothelial venules (HEV) with evidence of an active germinal centre. Transcriptomic analysis confirmed the existence of a supportive microenvironment conducive to TLO formation and maintenance. Furthermore, TLO formation was also observed in human cauda epididymidis f …

Снижение уровня эстрогена лимфатического узла вызывает неиспускающее заболевание аутоиммунной энцефаломиелита.

Эстроген, стероидный гормон, синтезированный как гонадальными, так и негональными тканями, играет ключевую роль в модуляции иммунной реакции, включая снижение частоты рецидивов при повторном излучающем многократном склерозе (МС). В этом исследовании изучалось выражение ароматазы, фермента, ответственного за синтез эстрогенов, в лимфатических узлах (ЛН) и его потенциальная роль в патогенезе MS с использованием модели мыши. Мы использовали мыши Cyp19-RFP, где клетки, которые выражают или ранее выражали ген Cyp19 (кодирование ароматазы), обозначаются красным флуоресцентным белком (RFP). RPF был о

Совпадение в аннотации оригинала
Estrogen, a steroid hormone synthesized by both gonadal and nongonadal tissues, plays a pivotal role in modulating immune responses, includi … ng aromatase) are marked by red fluorescent protein (RFP). RFP was detected in the high endothelial venules of all morphologically identifiable LNs, indicating aromatase activity within these tissues. We discovered that LNs actively synthesize 17β-estradiol, but this activity declines with age. Targeted delivery of an aromatase inhibitor specifically to L …

Systemic capillary leak syndrome.

The vascular endothelial barrier maintains intravascular volume and metabolic homeostasis. Although plasma fluids and proteins extravasate continuously from tissue microvasculature (capillaries, post-capillary venules), systemic vascular leakage increases in critical illness associated with sepsis, burns and trauma, among others, or in association with certain drugs or toxin exposures. Systemically dysregulated fluid homeostasis, which can lead to hypovolaemia, hypotensive shock and widespread tissue oedema, has been termed systemic capillary leak syndrome (SCLS) when overt secondary causes (f…

The first report of leukocytoclastic vasculitis induced by once-weekly subcutaneous semaglutide.

INTRODUCTION: Leukocytoclastic vasculitis (LCV) is a small vessel vasculitis involving arterioles, capillaries and postcapillary venules. LCV is generally confined to the skin, with extracutaneous manifestations occurring less frequently. LCV has multiple potential etiologies. Indeed, histological LCV can be found in anti-neutrophil cytoplasmic antibody (ANCA)-associated vasculitis, immune complex vasculitis, vasculitis associated with systemic diseases (i.e. sarcoidosis, Sjögren's syndrome, rheumatoid arthritis, and systemic lupus erythematosus), or in vasculitis associated with cancer, infec…

Microenvironmental Changes in Mediastinal Fat-associated Lymphoid Clusters and Lungs in Early and Late Stages of Metastatic Lung Cancer Induction.

The prognosis of metastatic lung melanoma (MLM) has been reported to be poor. An increasing number of studies have reported the function of … ytes, CD8+ cytotoxic T-cells, CD16+ natural killer (NK) cells populations, area of high endothelial venules, and lung lymphatic vessels in cancer groups at both the stages as compared with the PBS groups. Furthermore, a significant positive correlation was observed between immune cell populations in MFALCs and the lungs (B- and T-lymphocytes, and NK cells …

Compartment 1 acidosis associated with hemorrhagic diathesis causing mortalities among racing camels in Oman.

Camels are adapted to digestion of dry rough forages for their nutrition, and sudden change to highly digestible feed during the racing seas … tinal tract (compartment 3 and colon), lungs, and heart. Fibrin thrombi in arterioles, capillaries, venules, and medium-sized veins were observed especially in the pulmonary interstitium, submucosa of the large intestine (ascending colon), deep dermis, and renal cortex. Furthermore, widespread hemorrhages and necrosis were constant histopathological lesio …

Frosted branched angiitis due to viral meningo-encephalitis and ocular toxoplasmosis; a rare case report from Nepal.

UNLABELLED: Frosted branched angiitis (FBA) is characteristic florid translucent retinal perivascular sheathing of both arterioles and venules in association with variable uveitis and vasculitis affecting the entire retina. The vascular sheathing is supposed to be an immune-mediated reaction, possibly due to immune complex deposition in vessel walls secondary to various underlying etiologies. The authors aim to report a case of FBA secondary to herpes simplex virus and Toxoplasma gondii infection causing the diagnostic dilemma. This is the first case report on FBA from Nepal. CASE REPORT: An …

ANOMALIES OF CHOROIDAL VENOUS STRUCTURE IN HIGHLY MYOPIC EYES.

PURPOSE: To identify anomalies of choroidal venous structure in highly myopic (HM) eyes. METHODS: Widefield indocyanine green angiographic … ffected by steeper staphyloma edges and anastomoses were observed through large trunks and terminal venules. In two eyes with large trunk anastomosis, attenuation of the less dominant vortex vein was observed afterward. CONCLUSION: Choroidal venous anomalies are more common in HM eyes than controls. Choroidal venous structure in HM eyes may be altering c …

Intensity distribution segmentation in ultrafast Doppler combined with scanning laser confocal microscopy for assessing vascular changes associated with ageing in murine hippocampi.

The hippocampus plays an important role in learning and memory, requiring high-neuronal oxygenation. Understanding the relationship between … ble to associate CBV with vascular structure-and track its longitudinal changes-at the artery-vein, venules, arteriole, and capillary levels. We believe that this combined approach can be a powerful tool for studying other acute (e.g., brain injuries), progressive (e.g., neurodegeneration) or induced pathological changes.

Novel intragraft regulatory lymphoid structures in kidney allograft tolerance.

Intragraft events thought to be relevant to the development of tolerance are here subjected to a comprehensive mechanistic study during long … rm in response to chronic viral infection and transgenic Lta expression, TOLS lack high endothelial venules and germinal centers. TOLS represent a novel, pathogenetically important type of TLO that are in situ markers of regulatory tolerance.

Retinal nerve fibre layer and peripapillary vascular density by optical coherence tomography angiography in schizophrenia.

Clinical relevance: Schizophrenia is a progressive disease. Analysis of retinal nerve fibre layer and peripapillary vascular density by optical coherence tomography angiography in schizophrenia patients may help to explain the pathophysiology of the disease.Background: Both macular and optic nerve microvascular structures can be visualised with optical coherence tomography angiography. Few studies have evaluated retinal vessels in patients with schizophrenia. Some of these studies suggest that larger retinal venules are associated with the severity of psychotic symptoms.Methods: This study eva…

Distinctive Subpopulations of Stromal Cells Are Present in Human Lymph Nodes Infiltrated with Melanoma.

Metastasis of human tumors to lymph nodes (LN) is a universally negative prognostic factor. LN stromal cells (SC) play a crucial role in ena … o subsets of human LN pericytes, CD90+ CD146+ CD36+ NG2- pericytes in the walls of high endothelial venules and other small vessels, and CD90+ CD146+ NG2+ CD36- pericytes in the walls of larger vessels. Distinguishing between these CD90+ SC subpopulations in human LNs allows for further study of their respective impact on T-cell responses to tumor antigen …

Clinical Features and Experimental Models of Cerebral Small Vessel Disease.

Cerebral small vessel disease (SVD) refers to a group of disease conditions affecting the cerebral small vessels, which include the small arteries, arterioles, capillaries, and postcapillary venules in the brain. SVD is the primary cause of vascular cognitive impairment and gait disturbances in aged people. There are several types of SVD, though arteriolosclerosis, which is mainly associated with hypertension, aging, and diabetes mellitus, and cerebral amyloid angiopathy (CAA) comprise most SVD cases. The pathology of arteriolosclerosis-induced SVD is characterized by fibrinoid necrosis and li…

Angioimmunoblastic T-cell lymphoma-like lymphadenopathy in mice transgenic for human RHOA with p.Gly17Val mutation.

A missense mutation in RHOA encoding p.Gly17 Val has been reported to occur frequently in angioimmunoblastic T-cell lymphoma (AITL). Here, we describe a murine model which expresses the human RHOA mutant gene product in a T-cell specific manner and develops AITL-like symptoms. Most transgenic mice feature with latency one or two enlarged lymph nodes characterized by aberrant lymph node architecture, extensive lymphocytic infiltration, extrafollicular meshwork of follicular dendritic cells (FDC) and arborized endothelial venules. Importantly, we provide evidence for expansion of PD-1+ follicula…

Long term outcomes from lymphatic venous anastomosis after total hysterectomy to prevent postoperative lymphedema in lower limb.

BACKGROUND: Lymphedema in lower limb is one of major postoperative complications followed by a total hysterectomy with lymph node dissection. The objective of this report is to examine a long-term result of lymphaticovenous anastomosis procedure as a preventive surgery. METHODS: Sixteen patients with endometrial cancer underwent an abdominal hysterectomy with a bilateral salpingo-oophorectomy. Just after pelvic lymph node dissection, either end-to-end or sleeve anastomosis utilizing venules and suprainguinal lymph vessels was performed. During the observation period from 4 to 13 years, the sy…