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In vivo evaluation of biocompatibility and biodegradation of porcine collagen membranes.

INTRODUCTION: Collagen-based materials differ in absorption time, biodegradation patterns, and inflammatory cell infiltration. This study aimed to evaluate the biocompatibility and biodegradation of native, differently processed, and cross-linked porcine collagen membranes implanted in the subcutaneous tissue of rats, following ISO 10993-6:2016. METHODS: Sixty Sprague-Dawley rats were randomly divided into four groups: Group 1 (lyophilized 3 % porcine type I collagen membrane), Group 2 (lyophilized 3 % porcine type I collagen membrane, dehydrothermal [DHT]), Group 3 (1,4-butanediol diglycidyl…

The administration of exogenous HSP47 as a collagen-specific therapeutic approach.

The proof of principle of the therapeutic potential of heat shock protein 47 (HSP47) for diseases characterized by defects in collagen I synthesis is here demonstrated in osteogenesis imperfecta (OI), a prototype of collagen disorders. Most of the OI mutations delay collagen I chain folding, increasing their exposure to posttranslational modifications that affect collagen secretion and impact extracellular matrix fibril assembly. As a model, we used primary fibroblasts from OI individuals with a defect in the collagen prolyl 3-hydroxylation complex, since they are characterized by the synthesi…

Evolutionary Patterns of Collagen Fiber Arrangement and Calcification in Atherosclerosis.

Collagen is a major structural component of human tissues, and its spatial arrangement is critical for maintaining tissue mechanical integrity and regulating cell behavior. In atherosclerosis (AS), collagen fiber arrangement has been implicated in plaque stability and the regulation of vascular calcification, yet its evolution during disease progression and association with other pathological processes remain poorly understood. In this study, male ApoE-/- mice were fed a high-fat diet to establish a model of AS. Histological staining was performed every 5 weeks to analyze the collagen fiber al…

Treatment of Cutaneous Collagenous Vasculopathy With Pulsed Dye Laser.

OBJECTIVES: Cutaneous collagenous vasculopathy (CCV) is a rare, idiopathic microangiopathy marked by widespread telangiectasias and histologic vessel wall thickening. Due to its rarity and clinical overlap with other vascular conditions, CCV poses diagnostic and therapeutic challenges. This report highlights the successful use of pulsed dye laser (PDL) therapy in treating a case of extensive CCV. METHODS: A 40-year-old woman with a decade-long history of progressive telangiectasias involving the extremities, trunk, and forearms underwent treatment with a 595-nm PDL. Six treatment sessions wer…

Роль коллагена типа I в травме белой материи.

Увечье белой материи - патология мозга, отмеченная потерей олигодендроцитов и результирующим демиэлированием. Различные механизмы вызывают травму белой материи, включая ишемический инсульт и множественный склероз. Олигодендроциты регенерируют белую материю в центральной нервной системе в процессе, называемом ремиелинацией, волшебным демиелином аксонов с новым миелином. Несмотря на то, что сбой в ремии можно наблюдать при постепенном множественном склерозе и после ишемического инсульта, механизмы регенерации поврежденной белой материи остаются неясными. В этом обзоре мы в первую очередь концент

Совпадение в аннотации оригинала
White matter injury is a cerebral pathology marked by the loss of oligodendrocytes and the resultant demyelination. Various mechanisms induc … affecting white matter regeneration. In addition, we provide recent findings on the role of type I collagen as an inhibitory molecule of remyelination in white matter lesions.

Collagenous gastritis with elevated fecal calprotectin in a pediatric patient.

Collagenous gastritis is a rare and chronic inflammatory condition of undetermined etiology characterized histologically by thickened subepithelial collagen bands and increased intraepithelial lymphocytes. Here, we present a collagenous gastritis case in a 16-year-old female with chronic abdominal pain, persistently elevated fecal calprotectin (507 and 796 mcg/g), and resolved iron deficiency anemia. The patient's history, laboratory tests, endoscopy, and magnetic resonance imaging ruled out common causes of elevated fecal calprotectin, including Helicobacter pylori and gastrointestinal infect…

Factors Affecting the Evaluation of Collagen Deposition and Fibrosis In Vitro.

Immune responses to biomedical implants, wound healing, and diseased tissues often involve collagen deposition by fibroblasts and other stromal cells. Dysregulated collagen deposition can lead to complications, such as biomaterial fibrosis, cardiac fibrosis, desmoplasia, liver fibrosis, and pulmonary fibrosis, which can ultimately result in losses of organ function or failure of biomedical implants. Current in vitro methods to induce collagen deposition include growing the cells under macromolecular crowding conditions or on fibronectin-coated surfaces. However, the majority of these methods h…

Successful Collagen Crosslinking in a Patient With Keratoconus and Systemic Scleroderma.

Scleroderma is an autoimmune disease that affects connective tissue. Keratoconus (KC) is a rare ocular condition that may appear alongside scleroderma. Contact lenses are an essential visual aid for KC patients, especially in advanced cases. However, scleroderma patients may face difficulties using them due to finger-related disabilities. Corneal collagen cross-linking (CXL) is a crucial treatment used to prevent corneal thinning and visual deterioration in progressive KC. However, the potential trigger of corneal melt and delayed healing following CXL in KC patients with scleroderma is a matt…

A case of collagenous gastritis and literature review: A case report.

RATIONAL: Collagenous gastritis (CG) is rarely encountered in clinical practice. Here, we reported a case of CG with iron-deficiency anemia as the main symptom. PATIENT CONCERNS: The patient was a 26-year-old woman who sought medical advice with a chief complaint of recurrent upper abdominal distention and anemia since the last 3 years. DIAGNOSES: Gastroscopy at admission showed diffuse nodular mucosa. The pathology showed the formation of a belt hyperplasia of collagen in the superficial mucosa along with the infiltration of inflammatory cells. The subepithelial collagen band was 17.68 to 3…

The collagen matrix regulates the survival and function of pancreatic islets.

The extracellular matrix (ECM) provides an appropriate microenvironment for many kinds of cells, including pancreatic cells. Collagens are the most abundant components of the ECM. Type I, IV, V and VI collagen has been detected in pancreatic islets, and each type plays important role in the proliferation, survival, function and differentiation of pancreatic cells. In some cases, collagens show behaviours similar to those of growth factors and regulate the biological behaviour of β cells by binding with certain growth factors, including IGFs, EGFs and FGFs. The transcriptional coactivator YAP/T…

Advances in the authentication of collagen products based on DNA technology.

Collagenous products are making their way into consumer markets such as foods, nutraceuticals, cosmetics and pharmaceuticals increasingly. Collagen in a large family of proteins is ubiquitous in metazoan. The most effective way to identify biological samples including collagen is DNA technology indisputably. However, the DNA content of collagen mostly derived from connective tissue is relatively less, and commercial collagen products are usually subjected to some harsh treatments in the production process, which makes DNA damage more serious, thus tracing their origin becomes a huge challenge.…

Development and Characterization of a AuNP-Genipin-Viscoelastic Collagen Material.

Lower back pain is one of the leading causes of disability, affecting 11.9% of the population worldwide and studies have shown that intervertebral disc degeneration is a common cause for chronic lower back pain. We have explored the combination of three components, viscoelastic collagen, genipin, and gold nanoparticles to determine its potential to promote regeneration of the intervertebral disc, specifically for nucleus pulposus regeneration. The goal of this study was to develop, fabricate and characterize different formulations of viscoelastic collagen conjugated with gold nanoparticles and…

Atypical Presentations of Collagenous Gastritis Mimicking Celiac Sprue.

Collagenous gastritis has been reported as a rare cause of nausea, diarrhea, weight changes, and early satiety in female patients. Here, we describe two women aged 43 and 71 years who presented with similar symptoms. Gastric biopsies from both individuals showed thickened, irregular subepithelial collagen bands (>10 μm). The pathogenesis of collagenous gastritis is poorly understood, but it may be the presenting symptom for many underlying autoimmune conditions. In particular, there is a well-established connection between collagenous disorders of the gastrointestinal tract and celiac sprue, S…

Collagen and Microvascularization in Placentas From Young and Older Mares.

In older mares, increasing collagen fibers (fibrosis) in the endometrium and oviduct predisposes to sub-fertility and infertility. In this study, (i) gene transcription of collagen (qPCR: COL1A1, COL1A2, COL3A1, COL5A1); (ii) total collagen protein (hydroxyproline); (iii) collagen distribution (Picrosirius red staining; polarized light microscopy); and (iv) microvascular density (Periodic acid-Schiff staining), were evaluated in mares' placenta, and related to mares age, and placenta and neonate weights. Samples were collected from the gravid horn, non-gravid horn, and body of the placenta fro…

Collagen and elastic fiber remodeling in the pregnant mouse myometrium†.

The myometrium undergoes progressive tissue remodeling from early to late pregnancy to support fetal growth and transitions to the contractile phase to deliver a baby at term. Much of our effort has been focused on understanding the functional role of myometrial smooth muscle cells, but the role of extracellular matrix is not clear. This study was aimed to demonstrate the expression profile of sub-sets of genes involved in the synthesis, processing, and assembly of collagen and elastic fibers, their structural remodeling during pregnancy, and hormonal regulation. Myometrial tissues were isolat…

Immobilization of Collagen on the Surface of a PEEK Implant with Monolayer Nanopores.

Polyetheretherketone (PEEK) is the only polymer material that can replace titanium implants in the field of orthopedics. This is because the … surface by contacting PEEK with a mixed acid under ultrasound. Additionally, by immobilizing type I collagen on the porous PEEK surface through dopamine coating, it was possible to obtain collagen-immobilized porous PEEK (P-PEEK-Col) with high compatibility with osteoblasts. This P-PEEK-Col has high potential for use as a bone substitute that promotes bone …