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Hypermethylation of OPRM1: Deregulation of the Endogenous Opioid Pathway in Myalgic Encephalomyelitis/Chronic Fatigue Syndrome and Fibromyalgia.

Myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS) and fibromyalgia (FM) are debilitating disorders with overlapping symptoms such as chronic pain and fatigue. Dysregulation of the endogenous opioid system, particularly µ-opioid receptor function, may contribute to their pathophysiology. This study examined whether epigenetic modifications, specifically µ-opioid receptor 1 gene (OPRM1) promoter methylation, play a role in this dysfunction. Using a repeated-measures design, 28 ME/CFS/FM patients and 26 matched healthy controls visited the hospital twice within four days. Assessments in…

Regnase-1 in cDC1 controls T cell priming and shapes the dynamics of experimental autoimmune encephalomyelitis.

INTRODUCTION: Multiple sclerosis is a chronic inflammatory demyelinating disease of the central nervous system (CNS), in which various immune cells contribute to disease progression, yet the role of dendritic cells (DCs) remains incompletely understood. The RNA-binding protein Regnase-1 plays an important role in regulating immune cell function, but its function in DCs, particularly conventional type 1 DCs (cDC1), has not been defined. METHODS: We investigated the role of Regnase-1 in cDC1 and its impact on experimental autoimmune encephalomyelitis (EAE) on Regnase-1 fl/+ Xcr1 -Cre+ and contr…

An adaptive cellular source of IL-17A and IL-17F is critical for the induction of experimental autoimmune encephalomyelitis.

T helper 17 (TH17) cells are thought to play an important role in the pathogenesis of many autoimmune disorders, including multiple sclerosis and its animal model, experimental autoimmune encephalomyelitis (EAE). However, recent studies have suggested that interleukin-17 (IL-17) may be dispensable for the induction of EAE. Here, we use an adoptive transfer model of EAE to demonstrate that an adaptive cellular source of IL-17 is absolutely required for the induction of EAE disease. We further show that B cells can regulate the transfer of EAE disease in this context. Transcriptional profiling o…

Immunoregulatory Effect of Curcumin on Experimental Autoimmune Encephalomyelitis (EAE), an Animal Model of Multiple Sclerosis (MS).

Demyelination and progressive neurodegeneration within the central nervous system (CNS) are hallmarks of multiple sclerosis (MS), a chronic autoimmune disease. This review critically assesses the therapeutic efficacy of curcumin-a polyphenolic compound from the rhizome of Curcuma longa turmeric-in experimental autoimmune encephalomyelitis, a well-recognized animal model of MS. The significant anti-inflammatory and antioxidant properties of curcumin help slow the development of EAE. By suppressing pro-inflammatory cytokines, including interleukin-12 (IL-12), interleukin-17 (IL-17), and interfer…

"I would love to say it's the fatigue but honestly it's not": Into Adulthood with Chronic Fatigue Syndrome/Myalgic Encephalomyelitis.

Chronic Fatigue Syndrome/Myalgic Encephalomyelitis (CFS/ME) is a debilitating and poorly understood condition which interferes with adolescents' typical development. This study aimed to explore the experience of transitioning into adulthood from the perspective of adolescents and young adults (16-25yo) with CFS/ME. Thirteen young people recruited through a community allied health clinic in Victoria, Australia, participated in semi-structured interviews. Interviews were analysed using reflexive thematic analysis and produced the following themes: Independence: a different path or an inaccessibl…

Adult-Onset Acute Disseminated Encephalomyelitis in Elderly Filipino Patients: A Case Report and Review of the Literature.

Acute disseminated encephalomyelitis (ADEM) is an autoimmune disease characterized by inflammation of the cerebral hemispheres, brainstem, cerebellum, and spinal cord, often preceded by prodromal infections or vaccinations. The mainstay of treatment is immunosuppression with high-dose steroid therapy, intravenous immunoglobulin (IVIg), or plasma exchange (PE). While most pediatric patients improve with treatment, the prognosis in adults is less favorable. Local studies reporting the epidemiology, prevalence, incidence, treatment outcomes, and prognosis of ADEM are scarce. This case series pres…

Circulating cell-free RNA signatures for the characterization and diagnosis of myalgic encephalomyelitis/chronic fatigue syndrome.

People living with myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS) experience heterogeneous and debilitating symptoms that lack sufficient biological explanation, compounded by the absence of accurate, noninvasive diagnostic tools. To address these challenges, we explored circulating cell-free RNA (cfRNA) as a blood-borne bioanalyte to monitor ME/CFS. cfRNA is released into the bloodstream during cellular turnover and reflects dynamic changes in gene expression, cellular signaling, and tissue-specific processes. We profiled cfRNA in plasma by RNA sequencing for 93 ME/CFS cases and …

The association of fatigue and pain with cognitive test performance in patients with myalgic encephalomyelitis/chronic fatigue syndrome.

OBJECTIVES: Patients with myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS) typically perform worse on cognitive tasks compared to controls. The present study explored the independent associations of fatigue and pain symptoms with cognitive performance in a large sample of patients who met CDC criteria of CFS (n = 1375), of whom most also met NICE/ IOM criteria (n = 1072). Moreover, we tested the hypothesis that these associations become stronger with older age and longer symptom duration. METHODS: Questionnaires and diaries were employed assessing fatigue and pain severity, togethe…

The pivotal role of central sensitization in long COVID, fibromyalgia and myalgic encephalomyelitis/chronic fatigue syndrome.

INTRODUCTION: Long COVID is a condition characterized by persistent unexplained symptoms following COVID-19 infection. These symptoms are not related to another disease or organ damage and are similar to those in fibromyalgia and myslgic encephalomyelitis/chronic fatigue syndrome (ME/CFS). AREAS COVERED: The similar clinical and pathophysiological features and management of long COVID, fibromyalgia and ME/CFS are explored from the unifying framework of central sensitivity syndromes. The article is based on a literature search utilizing PubMed for content published between 2021 and 1 May 2025,…

Транскриптомные и функциональные наборы аннотаций для лечения пептида GV1001 в экспериментальной аутоиммунной энцефаломиелитной модели мыши.

Этот набор данных содержит транскриптивные и функциональные аннотационные данные, полученные из тканей спинного мозга мышей с экспериментальным аутоиммунным энцефаломиелитом (EAE), обработанным GV1001, т.е. Для выявления дифференциально выраженных генов (DEG) между группами GV1001-обработанными и обработанными транспортными средствами была проведена последовательность навалом РНК. Полученные в результате ДЭГ были подвергнуты анализу на предмет обогащения траектории Ген Онтологического процесса (GOBP) и KEGG и были дополнительно аннотированы по типам клеток с использованием справочных одноклето

Совпадение в аннотации оригинала
This dataset presents transcriptomic and functional annotation data derived from spinal cord tissues of mice with experimental autoimmune encephalomyelitis (EAE) treated with GV1001, a telomerase-derived peptide. Bulk RNA sequencing was performed to identify differentially expressed genes (DEGs) between GV1001-treated and vehicle-treated groups. The resultin…

AAV1.NT3 генная терапия смягчает остроту аутоиммунного энцефаломиэлита в модели мыши для множественных склерозов.

Множественный склероз (MS) — хроническое воспалительное и нейродегенеративное заболевание центральной нервной системы (КНС), которое оказывает воздействие более чем на организм человека. 2.5 million Пациенты по всему миру. Хроническое обезвоживание в ЦНС играет важную роль в увековечении аксоновых потерь и увеличивает трудности в поощрении воспоминаний. Поэтому стратегии регенерации и нейрозащиты имеют важное значение для преодоления этого препятствия для сохранения аксоновой целостности и функции. Нейропофин 3 (В тыс.NT-3) обладает иммунодуляционными и антивоспалительными свойствами, помимо ш

Совпадение в аннотации оригинала
Multiple sclerosis (MS) is an immune-mediated chronic inflammatory and neurodegenerative disease of the central nervous system (CNS) affecti … r this study, scAAV1.tMCK.NT-3 was delivered to the gastrocnemius muscle of experimental autoimmune encephalomyelitis (EAE) mice, the chronic relapsing mouse model of MS, at 3 weeks post EAE induction. Measurable NT-3 levels were found in serum at 7-weeks post gene delivery. The treated cohort showed improved clinical scores and performed significantly better in ro …

Dabrafenib mitigates the neuroinflammation caused by ferroptosis in experimental autoimmune encephalomyelitis by up regulating Axl receptor.

Multiple sclerosis is an autoimmune disease that causes inflammatory damage to the central nervous system. At present, the pathogenesis of the disease is unknown. There is a lack of few effective therapy medications available. Therefore, it is necessary to further explore the pathogenesis of this illness and develop potential therapeutic drugs. Dabrafenib is potential therapeutic medicine for nervous system disease. In this study, we preliminarily studied the possible mechanism of dabrafenib in the treatment of multiple sclerosis from the perspective of ferroptosis. First, we observed that dab

Varicella zoster infection-associated progressive encephalomyelitis with rigidity and myoclonus in a 6-month follow-up.

A patient with reactivated varicella zoster virus (VZV) manifesting in the left-sided dermatome L3 and S2-S4 developed tonic spasms which mo … signs of myelitis and EEG was negative for epilepsy. However, on clinical presentation, progressive encephalomyelitis with rigidity and myoclonus, a variant of the more well-known stiff person syndrome, was suspected and later supported by positive serum testing for associated anti-glycine receptor antibodies. Initially established antiviral, anti-epileptic and sym …

Multiphasic Acute Disseminated Encephalomyelitis with a Temporal Gap of 26 Years: A Case Report with Novel Presentation.

INTRODUCTION: Acute disseminated encephalomyelitis (ADEM) is an acute autoimmune demyelinating disease of the central nervous system that typically follows an acute viral infection or post-vaccination. It is more common in children than in adults and is characterized by widespread demyelination of the white matter of the brain and spinal cord. ADEM typically presents as a monophasic illness. Multiphasic ADEM has been an emerging topic with case reports highlighting the importance of the need for further investigation and literature on this topic. Multiphasic ADEM in adults is a rare presentati…

Лупин (Lupinus angustifolius) Протеин гидролизат уменьшает остроту экспериментального аутоиммунного энцефаломелита: предварительное исследование.

Множественный склероз (MS) — нейродегенеративное заболевание, основным источником которого является воспаление и окислительный стресс в центральной нервной системе. Существует много лекарств, которые уменьшают клинические признаки MS, но ни один из них не лечит болезнь. Было доказано, что пищевые белки содержат зашифрованные пептиды, которые могут высвобождаться после гидролиза и осуществлять многочисленные биологические виды деятельности. Недавно мы продемонстрировали антивоспалительные и антиоксидантные действия волчьего белка гидролизата (LPH) как in vitro, так и in vivo. Поэтому цель этого

Совпадение в аннотации оригинала
Multiple sclerosis (MS) is a neurodegenerative disease, with inflammation and oxidative stress in the central nervous system being the main … dy was to evaluate whether LPH is capable of reducing the clinical signs of experimental autoimmune encephalomyelitis (EAE), a mouse model of MS. EAE was induced in female C57BL/6N mice and they were treated intragastrically with LPH (100 mg/kg) or vehicle (control group) from day 0 (prophylactic approach) or from the onset of the disease (day 12 post-induction; th …

Nogo-A antibody delivery through the olfactory mucosa mitigates experimental autoimmune encephalomyelitis in the mouse CNS.

Systemic administration of Nogo-A-neutralizing antibody ameliorates experimental autoimmune encephalomyelitis (EAE), an animal model of multiple sclerosis. However, the blood-brain barrier (BBB) is a major obstacle limiting the passage of systemically applied antibody to the CNS. To bypass the BBB, in the present study we tested the intranasal route of administration by targeting the olfactory mucosa with the Nogo-A-blocking antibody 11C7 mAb in myelin oligodendrocyte glycoprotein-induced EAE. Antibodies were specifically administered onto the olfactory mucosa using a microcatheter. Antibody d…

Cardiovascular and haematological pathology in myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS): A role for viruses.

ME/CFS is a debilitating chronic condition that often develops after viral or bacterial infection. Insight from the study of Long COVID/Post Acute Sequelae of COVID-19 (PASC), the post-viral syndrome associated with SARS-CoV-2 infection, might prove to be useful for understanding pathophysiological mechanisms of ME/CFS. Disease presentation is similar between the two conditions, and a subset of Long COVID patients meet the diagnostic criteria for ME/CFS. Since Long COVID is characterized by significant vascular pathology - including endothelial dysfunction, coagulopathy, and vascular dysregula

Human rabies encephalomyelitis in the background of rabies outbreak in animals in Gelephu, Bhutan, 2023: a case report.

BACKGROUND: Rabies continues to pose significant public health challenges in many developing countries including Bhutan. A probable case of rabies was admitted to our hospital and its reporting led to the uncovering of an outbreak in domestic and wild animals. We discuss the challenges in the diagnosis and management of rabies in a resource-limited setting. CASE PRESENTATION: A 35-year-old male presented with intermittent fever, bilateral lower limb weakness that was rapidly progressive, urinary incontinence with episodes of palpitations and sweating. He had sustained a Category III bite on t

The antimicrobial peptide cathelicidin drives development of experimental autoimmune encephalomyelitis in mice by affecting Th17 differentiation.

Multiple sclerosis (MS) is a highly prevalent demyelinating autoimmune condition; the mechanisms regulating its severity and progression are unclear. The IL-17-producing Th17 subset of T cells has been widely implicated in MS and in the mouse model, experimental autoimmune encephalomyelitis (EAE). However, the differentiation and regulation of Th17 cells during EAE remain incompletely understood. Although evidence is mounting that the antimicrobial peptide cathelicidin profoundly affects early T cell differentiation, no studies have looked at its role in longer-term T cell responses. Now, we r…