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[Pathology of the human apolipoprotein E gene].

Apolipoprotein E (apo E) is a polymorphic glycoprotein that plays an essential part in the binding to receptors for the uptake of chylomicro … different approaches. The first one is based on the separation of plasma isoforms of the protein by isoelectric focusing or bidimensional electrophoresis. The other one consists in the application of molecular biology techniques (PCR and endonuclease restriction profiles) for a detection of the common alleles and of several rare alleles, avoiding the possible errors o …

Mutations affecting the activity of toxic shock syndrome toxin-1.

Toxic shock syndrome toxin-1 (TSST-1), the potent staphylococcal exoprotein linked to most cases of the toxic shock syndrome, is a V beta-re … pernants of a nontoxigenic strain of S. aureus using a combination of ultrafiltration, liquid-phase isoelectric focusing, and ion-exchange chromatography. The mutants retained global structural integrity as evidenced by circular dichroism spectroscopy, their preserved resistance to trypsin digestion, and their preserved binding to a neutralizing murine monoclonal anti …

Update: new guidelines for the treatment of infants with sickle cell disease. Agency for Health Care Policy and Research.

These clinical practice guidelines set forth a comprehensive program for identifying, diagnosing, and treating newborns and infants with sic … or liquid blood samples should be used for hemoglobinopathy screening. Hemoglobin electrophoresis, isoelectric focusing, and high performance liquid chromatography are acceptable, reliable, and accurate testing methods. Infants identified on initial screening must be retested to establish a definitive diagnosis. Affected infants must be given twice-daily oral penicil …

Corneal aldehyde dehydrogenase, glutathione reductase, and glutathione S-transferase in pathologic corneas.

Previously we have reported that various pathologic corneas exhibited a "diseased" two-band corneal aldehyde dehydrogenase (ALDH) zymogram a … zymatic behavior that was in concordance with our earlier result in the corneal ALDH zymogram after isoelectric focusing. Furthermore, analysis of our several studies tends to support the proposed structural function of ALDH in human cornea.

[Immediate allergic reaction to natural latex with special reference to surgical gloves].

There is an increasing incidence of contact urticaria (CU) and systemic reactions to rubber products. 34 patients are presented: Most were a … ately 28 kD. The Western Blot detected the 28 kD protein as allergen in the sera of three patients. Isoelectric focusing (IEF) proved no protein bands. Immunoprinting performed with sera of five patients presented allergen bands in an pH range between 3.8 and 4.55. This shows that radiostaining (immunoprint) is more sensitive than the Coomassie Blue staining. Although …

Apolipoprotein E allele frequencies in non-insulin-dependent diabetes mellitus with hypertriglyceridemia (type IIb, III, IV, and V hyperlipoproteinemia).

We examined apolipoprotein E (apo E) allele frequencies in non-insulin-dependent diabetes mellitus (NIDDM) patients with normolipidemia or various types of hypertriglyceridemia to elucidate the association of the apo E alleles with hypertriglyceridemia in NIDDM. NIDDM patients with normolipidemia (N = 134) or hypertriglyceridemia [type IIb hyperlipoproteinemia (HLP) (N = 42), III HLP (N = 7), IV HLP (N = 96), and V HLP (N = 8)] were randomly selected from our Diabetic Clinics. Apo E phenotypes (genotypes) were determined by our rapid flat-gel isoelectric focusing method. The frequency of the e…

Electrostatic interactions in wild-type and mutant recombinant human myoglobins.

Residue Val68 in human myoglobin has been replaced by Asn, Asp, and Glu with site-directed mutagenesis. Purified proteins were characterized by isoelectric focusing and by absorption, CD, and NMR spectroscopy. These studies demonstrated that Mb is able to tolerate substitution of the buried hydrophobic residue Val68 by Asn, Asp, and Glu. In the metaquo derivatives of the Glu and Asp mutants, the negative charge at residue 68 is stabilized by a favorable Coulombic interaction with the heme iron. In the absence of this interaction, as in the metcyano and ferrous deoxy derivatives, the relatively…

Generation of monoclonal murine anti-DNP-IgE, IgM and IgG1 antibodies: biochemical and biological characterization.

Monoclonal DNP-specific IgG (lambda 2 epsilon 2), IgM (kappa 2 mu2) and IgG [kappa 2 (gamma 1)2] were isolated fom the culture supernatant o … ed by equilibrium dialysis were 0 . 87 X 10(7) l/mol for IgE and 1 . 91 X 10(8) 1/mol for IgG1. The isoelectric focusing of the purified monoclonal antibodies revealed for IgG1 seven bands at a pH range of 6 . 3 - 7 . 2 and for IgE sixteen bands at a pH range of 4 . 5 to 6 . 8. the binding of 125I-anti-IgE to rat basophilic leukaemia (RBL) and rat mast cells which had …

Stabilization of neocarzinostatin nonprotein chromophore activity by interaction with apoprotein and with HeLa cells.

The methanol-extracted, nonprotein chromophore of the protein antibiotic neocarzinostatin (NCS), which possesses the full in vitro and in vi … and not the apoprotein, is inactivated by heat or light (360 nm) as determined by both activity and isoelectric focusing experiments. In contrast to other chromophoric antibiotic substances (daunorubicin and the extracted chromophore of aurodomomycin), the NCS chromophore interacts irreversibly with HeLa cells at 0 degrees C in serum-free medium so as to inhibit subse …

Bioinformatics-Driven Design of Fusion Recombinant Protein (EgFABP1-EgTeg) for Enhanced Immunodiagnosis of Hydatid Cysts.

BACKGROUND: Hydatid cyst disease is caused by the parasite Echinococcus and poses significant health concerns worldwide. Due to the lack of early symptoms and limited diagnostic tools, researchers aim to design a more specific and sensitive antigen. The study focuses on developing a recombinant multi-epitope antigen using two parasite proteins (EgTeg and EgFABP1) and the IH4 nanobody. METHODS: Protein sequences were analyzed and validated using bioinformatics tools, and B-cell epitopes were identified. The resulting antigen, confirmed by UniProt, is 266 amino acids long. RESULTS: The multi-e

Ethanol exposure alters protein expression in a mouse model of fetal alcohol spectrum disorders.

Alcohol exposure during development can result in variable growth retardation and facial dysmorphology known as fetal alcohol spectrum disorders. Although the mechanisms underlying the disorder are not fully understood, recent progress has been made that alcohol induces aberrant changes in gene expression and in the epigenome of embryos. To inform the gene and epigenetic changes in alcohol-induced teratology, we used whole-embryo culture to identify the alcohol-signature protein profile of neurulating C6 mice. Alcohol-treated and control cultures were homogenized, isoelectrically focused, and

Real-World Performance of CSF Kappa Free Light Chains in the 2024 McDonald Criteria.

OBJECTIVE: Kappa free light chains (KFLCs) in the cerebrospinal fluid (CSF) have a similar performance to CSF-restricted oligoclonal bands ( … CSF and serum KFLC and albumin were assayed with immunoturbidimetry. OCB status was assessed using isoelectric focusing. Eight KFLC metrics were tested: CSF KFLC, KFLC index, three population-based models of the upper limit for the CSF/serum kappa quotient corrected for CSF/serum albumin quotient, and their corresponding intrathecal fractions. RESULTS: The KFLC inde …

Intrathecal immunoglobulin production in the diagnosis, prognosis, and monitoring of autoimmune diseases of the central nervous system.

Intrathecal immunoglobulin production is a hallmark of central nervous system (CNS) inflammation and can be observed in autoimmune disorders of the CNS, primarily multiple sclerosis (MS), but also in other inflammatory conditions such as MOG antibody disease (MOGAD) and neuromyelitis optica spectrum disorder with aquaporin-4 antibodies (NMOSD-AQP4), as well as autoimmune encephalitis (AE). The assessment of intrathecal synthesis of immunoglobulins involves the detection of IgG oligoclonal bands (OCBs) via isoelectric focusing, the gold standard method, and the more recently developed automated…

Свободная от Каппы световая цепочка в качестве практической альтернативы олигокланным полосам в диагностике множественных склерозов.

ЦЕЛИ: Свободные цепочки освещения в Каппе стали надежным биомаркером для диагностики множественных склерозов (МС). По сравнению с олигоклональной полосой (ОКС) β-ФЛК обладает особыми преимуществами, в том числе более высокой доступностью в клинической практике. В этом исследовании оцениваются значения индекса ОВ-ФЛК у пациентов, страдающих НР, и изучается его потенциал в качестве практической альтернативы тесту на БМБ. МЕТОДЫ: концентрации цереброспинарной жидкости (CSF) и сыворотки Δ-FLC были определены с использованием иммунонефелометрического анализатора, в то время как анализ OCB проводилс

Совпадение в аннотации оригинала
OBJECTIVES: Kappa free light chains (κ-FLC) have emerged as a reliable biomarker for diagnosing multiple sclerosis (MS). Compared to oligocl … were quantified using an immunonephelometry analyzer, while OCB analysis was performed via agarose isoelectric focusing combined with immunoblotting. The cut-off values were set at ≥0.7 for the CSF IgG index and ≥6.6 for the κ-FLC index, with values exceeding these thresholds considered positive. κ -FLC index values were compared between OCB-negative and OCB-positive …

Kappa index in the diagnostic work-up of autoimmune encephalitis.

BACKGROUND: The presence of inflammatory changes in the cerebrospinal fluid (CSF), including immunoglobulin intrathecal synthesis (IS), can … with definite AE had pleocytosis and twelve (34%) showed CSF-restricted oligoclonal bands (OCB) at isoelectric focusing. The Kappa index was >6 in 29.4% and > 3 in 50% of the definite AE patients. It was elevated (>3) in 36.4% of patients with definite AE who resulted negative to OCB testing and was the only altered parameter suggestive of an ongoing inflammatory pro …

Repeated lumbar puncture in search of oligoclonal bands - What is the yield?

BACKGROUND: Cerebrospinal fluid (CSF) oligoclonal bands (OCBs) are immunoglobulins that represent intrathecal synthesis during central nervo … l syndromes who had repeated CSF samplings. OCBs were analyzed by agarose gel electrophoresis or by isoelectric focusing. RESULTS: During the years 2010-2020, we identified 48 patients with at least two CSF OCBs results in Rabin Medical Center. These included 11 patients with Multiple Sclerosis, ADEM and NMOSD (one patient each), 7 patients with unspecified demyelina …

Mutation-independent Proteomic Signatures of Pathological Progression in Murine Models of Duchenne Muscular Dystrophy.

The absence of the dystrophin protein in Duchenne muscular dystrophy (DMD) results in myofiber fragility and a plethora of downstream secondary pathologies. Although a variety of experimental therapies are in development, achieving effective treatments for DMD remains exceptionally challenging, not least because the pathological consequences of dystrophin loss are incompletely understood. Here we have performed proteome profiling in tibialis anterior muscles from two murine DMD models (mdx and mdx52) at three ages (8, 16, and 80 weeks of age), all n = 3. High-resolution isoelectric focusing li…

Cerebrospinal fluid oligoclonal bands in Neuroborreliosis are specific for Borrelia burgdorferi.

BACKGROUND: Cerebrospinal fluid (CSF) oligoclonal bands (OCB) occur in chronic or post-acute phase of inflammatory diseases of the central nervous system. OBJECTIVE: To determine whether CSF OCB in patients with neuroborreliosis (NB) are specific for borrelia burgdorferi senso lato. METHODS: We performed isoelectric focusing followed by immunoblotting in CSF of 10 NB patients and 11 controls (7 patients with multiple sclerosis, 2 patients with neuromyelitis optica spectrum disease, 1 patient with dementia and 1 patient with monoclonal gammopathy). Immunoblotting was performed using an uncoat…

Variation in the Protein Composition and Biological Activity of King Cobra (Ophiophagus hannah) Venoms.

The biochemical properties and biological activities of the venom from three individual Ophiophagus hannah (King cobra) specimens was compared. The toxicity against mice, the cytotoxicity against five cell lines, and the antioxidant activity were measured. The KV2 venom showed a higher cytotoxicity than the KV6 and the non-cytotoxic KV9 venoms. Comparative analysis of the O. hannah venom proteins was performed after 2-dimensional (2-D) denaturing gel electrophoresis and reverse phase high performance liquid chromatography (RP-HPLC). 2-D analysis by isoelectric focusing (IEF) Sodium dodecyl sul…

Clinical Effect of Alpha-1 Antitrypsin Deficiency in Antineutrophil Cytoplasmic Antibody-associated Vasculitis: Results from a French Retrospective Monocentric Cohort.

OBJECTIVE: Deficiency in alpha-1 antitrypsin (AAT) is a possible pathogenic cofactor in antineutrophil cytoplasmic antibody (ANCA)-associate … uary 2000 or September 2011, respectively, to June 2016. AAT dosage (nephelometry) and phenotyping (isoelectric focusing in agarose gel) were performed. RESULTS: Among the 142 patients with AAV, including 88 GPA and 54 MPA, 102 (72%) had the MM phenotype, 5 (4%) had a nonpolymerogenic M-variant phenotype, 18 (13%) had the deficient allele MZ, 12 (8%) had MS, 2 (1%) h …