Результаты поиска

Discovery of a highly specific radiolabeled antibody targeting B-cell maturation antigen: Applications in PET imaging of multiple myeloma.

PURPOSE: Multiple myeloma (MM) is characterized by the uncontrolled proliferation of monoclonal plasma cells (PC) in the bone marrow (BM). B … bearing mice to evaluate imaging performance and systemic distribution of [89Zr]Zr-DFO-BCMAh230430. Immunochemistry analysis was performed to detect BCMA expression in tumor tissues, confirming the specificity of our probe. Furthermore, we explored the anti-tumor efficacy of Lutetium-177 labeled BCMA antibody, [177Lu]Lu-DTPA-BCMAh230430, in tumor bearing-mice to …

Clear cell carcinoma of the urinary bladder: a report and comparison of four tumors of mullerian origin and nine of probable urothelial origin with discussion of histogenesis and diagnostic problems.

Carcinomas of the bladder that resemble clear cell carcinoma of mullerian type are rare. Whether such neoplasms 1) arise from mullerian elem … of clear cell carcinoma is of mullerian or transitional (urothelial) cell lineage, and at this time immunochemistry does not solve this problem.

A case report of metastatic hepatocellular carcinoma in the mandible and coracoid process: A rare presentation.

RationaleWe report a rare case of hepatocellular carcinoma (HCC) with metastases to the mandible and coracoid process of scapula without evidence of lung involvement.Patients concernsThe patient was diagnosed with HCC, presented 5 months later with right lower tooth pain, swelling over the right mandible area and right shoulder pain.DiagnosesHistopathological examination of mandible showed findings suggestive of metastatic HCC. Magnetic resonance imaging (MRI) of the right shoulder revealed findings of irregular enhancing lesion at the right coracoid process causing erosion of the coracoid pro

Совпадение в полном тексте источника
… Figure 6 Immunochemistry from the right mandible biopsy is positive for cytokeratin 20 (CK-20), 10×. Figure 7 CT head and neck showed heterogenous enhancing destructive soft tissue lesion in the angle of the right mandible (indicated by arrow). … Figure 4 Immunochemistry from the right mandible biopsy showing the tumor cells are strongly positive for pancytokeratin, 10×. Figure 5 Immunochemistry from the right mandible biopsy is positive for hepatocyte paraffin antigen-1 (HepPar-1), 10×. …

Diabetes abolishes sildenafil-induced cGMP-dependent protein kinase-I expression and cardioprotection.

The selective phosphodiesterase type 5 inhibitor sildenafil has been demonstrated to produce cardioprotection; however, diabetes is known to abolish cardioprotective signaling. We tested the hypothesis that sildenafil-induced cGMP-dependent protein kinase-I (PKG-I) expression and cardioprotection are attenuated by diabetes. Barbiturate-anesthetized dogs (n = 38) were instrumented for measurement of hemodynamics and subjected to 60-minute occlusion of the left anterior descending coronary artery and 3-hour reperfusion. Dogs were randomly assigned to receive 0.9% saline (control) or intravenous

[Isoenzymes, methodology and clinical significance (author's transl)].

In recent years the determination of serum enzyme activities has played an increasing role in clinical chemical diagnosis. Because the enzyme composition of single organs is qualitatively and, to a certain extent, quantitatively similar, the diagnostic value of enzyme activity determinations is often diminished. Each serum enzyme can be separated into isoenzymes which stem from different organs and make specific organ diagnoses possible. This separation is possible through chemico-physical and immunological methods. Electrophoretic, chromatographic and immunological methods for the determinati

Antigens of Pasteurella tularensis: preparative procedures.

Ether-water (EW) extraction of Pasteurella tularensis produced better antigens than five other chemical procedures. EW extracts produced from stationary-phase, liquid-grown, saline suspensions of strain SCHU S4 cells regularly induced agglutinin and precipitin formation in rabbits. Mice, guinea pigs, and monkeys also responded to EW extracts but with lower antibody levels. The use of strains of lower virulence, acetone-dried cells, organisms grown on a solid medium, and abbreviated extraction conditions all resulted in extracts with a diminished antigenicity, but logarithmic-phase and stationa

Cholecalcin (a 9-kDa cholecalciferol-induced calcium-binding protein) messenger RNA. Distribution and induction by calcitriol in the rat digestive tract.

In view of the possible physiological importance of the 9-kDa cholecalcin (a 9000-Mr cholecalciferol-induced calcium-binding protein) in the intestinal transport of calcium in mammals, the gene expression of this protein has been analysed. Its regulation in the digestive tract of the growing rat by calcitriol (1,25-dihydroxycholecalciferol) was studied using a specific cloned [32P]cDNA to 9-kDa cholecalcin. Northern hybridisation studies show that the cDNA sequence hybridises to a single 500-600-nucleotide species throughout the digestive tract and therefore demonstrate identical 9-kDa-choleca

[Suppressive effect of hydrogen sulfide donor on endothelin-1 production in aorta of atherosclerotic rats].

OBJECTIVE: To examine the effect of H2S donor, sodium hydrosulfide (NaHS), on ET-1 level in plasma and aorta in rats with atherosclerosis (A … and ET-1 content in aorta in rats of AS+NaHS group was decreased compared with AS group (P < 0.05). Immunochemistry results showed that ET expression in cytoplasm in aortic endothelial cells in rats of AS group was strengthened, while ET expression in rats of control group and AS+NaHS group was weak. NOS activity of rats in control group, AS group and AS+NaHS gro …

Overlapping expression patterns of the multiligand endocytic receptors cubilin and megalin in the CNS, sensory organs and developing epithelia of the rodent embryo.

Cubilin and megalin are multiligand epithelial endocytic receptors well characterized in the adult kidney and ileum where they form a complex essential for protein, lipid and vitamin uptake. Although inactivation of the megalin gene leads to holoprosencephaly and administration of anti-cubilin antibodies induces fetal resorptions or cranio-facial malformations their function in the developing embryo remains unclear. We recently showed that both proteins are strongly expressed by the maternal-fetal interfaces and the neuroepithelium of the early rodent embryo where they co-localize and form a c

Differentiation of Mouse Enteric Nervous System Progenitor Cells Is Controlled by Endothelin 3 and Requires Regulation of Ednrb by SOX10 and ZEB2.

BACKGROUND & AIMS: Maintenance and differentiation of progenitor cells in the developing enteric nervous system are controlled by molecules such as the signaling protein endothelin 3 (EDN3), its receptor (the endothelin receptor type B [EDNRB]), and the transcription factors SRY-box 10 (SOX10) and zinc finger E-box binding homeobox 2 (ZEB2). We used enteric progenitor cell (EPC) cultures and mice to study the roles of these proteins in enteric neurogenesis and their cross regulation. METHODS: We performed studies in mice with a Zeb2 loss-of-function mutation (Zeb2Δ) and mice carrying a sponta

Female genital tumors associated with human papillomavirus infection, and the concept of genital neoplasm-papilloma syndrome (GENPS).

With the recent development of new analytic methods, notably the DNA hybridization technique, many benign and malignant gynecologic tumors including carcinoma in situ, verrucous carcinoma and some invasive carcinomas of the vulva, the vagina and the cervix are found to be associated with human papillomavirus infection. Benign warts and multiple neoplasms frequently appear synchronously or metachronously in a single patient, and thus present as the genital neoplasm-papilloma syndrome (GENPS). Various methods of human papillomavirus identification and a spectrum of benign and malignant female ge

Epididymal cysteine-rich secretory proteins are required for epididymal sperm maturation and optimal sperm function.

STUDY QUESTION: What is the role of epididymal cysteine-rich secretory proteins (CRISPs) in male fertility? SUMMARY ANSWER: While epididyma … d methods. The knockout of individual genes was confirmed at a mRNA (quantitative PCR) and protein (immunochemistry) level. Fertility was assessed using breeding experiments and a histological assessment of testes and epididymal tissue. Sperm functional competence was assessed using a computer assisted sperm analyser, induction of the acrosome reaction using prog …

Cyclooxygenase-2 (COX-2) and hypoxia-induced factor 1α (HIF1α) expression in colorectal carcinoma and its correlation with other clinicopathological parameters.

BACKGROUND: Colorectal cancer is identified as the third-most prevalent cancer globally and the second leading cause of cancer mortality. METHODS: This study investigated the immunohistochemical expression of Cyclooxygenase-2 and Hypoxia-Induced Factor 1 alpha & correlated their expression with clinicopathological parameters in 100 retrospectively selected cases of colorectal adenocarcinoma from Port Said Governorate, Egypt. RESULTS: COX-2 was positively expressed in 67% of cases, and HIF-1α was positive in 57%. COX-2 positivity was statistically significantly associated only with lymph node

Association of MALAT1 rs619586 and rs3200401 single-nucleotide polymorphisms with breast cancer risk in Egyptian women.

BACKGROUND: MALAT1 (long non-coding RNA) is associated with growth and proliferation of breast cancer (BC). Functional single-nucleotide polymorphisms in MALAT1 may regulate an individual's susceptibility to cancer. Little is known regarding MALAT1 polymorphisms and the risk of BC in Egyptian women. OBJECTIVES: This study aims to determine the relationship between MALAT1 polymorphisms (rs619586 and rs3200401) and the risk of BC among Egyptian women, as well as clinicopathology and survival rates. METHODS: A case-control study was conducted involving 50 women with confirmed BC and 50 age-matc

Evaluation of CD14 expression in local recurrence in women with breast cancer (histopathological and immunohistochemical study).

Breast cancer (BC) is the most frequent cancer among women globally. CD14 high expression levels are associated with more aggressive behavior. The study aims to assess CD14 expressions in locally recurrent breast cancer and corresponding primary tumors whenever available, both intratumorally and peritumorally, and to analyze its association with clinicopathological features. This retrospective cross-sectional study included 55 cases of women with locally recurrent breast cancer. They were immunohistochemically stained using anti-CD14 antibody. Statistical associations between CD14 expression a

Evaluation of antithyroid antibodies and interferon gamma levels in euthyroid women with primary idiopathic infertility.

Primary idiopathic infertility remains challenging due to overlooked subclinical immunological factors. The purpose of this study was to examine the effects of interferon-gamma (IFN-γ) levels and Anti-Thyroid Peroxidase (Anti-TPO) antibodies on the reproductive outcomes of euthyroid women. The study included 90 participants (60 patients,30 controls). A comprehensive hormonal profile, including AMH, TSH, FSH, LH, prolactin, and estradiol, was assessed in all participants to ensure normal endocrine function. However, serum Anti-TPO levels exhibited a statistically significant elevation in the in

Unraveling the health effects of heavy metal exposure in women through biological fluid biomonitoring.

Heavy metal exposure is a major public health concern owing to its environmental persistence, bioaccumulation, and toxic effects on human health. Women are particularly susceptible to heavy metal toxicity because of physiological and hormonal factors that influence reproductive, endocrine, and metabolic functions. Exposure to metals such as lead, cadmium, mercury, and arsenic has been linked to reproductive disorders, hormonal imbalance, adverse pregnancy outcomes, and chronic diseases. For instance, elevated blood lead levels have been consistently associated with low birth weight and preterm

In silico design and evaluation of scFv-LukS-PV as a novel immunotoxin for AML therapy.

Acute myeloid leukemia (AML) remains a difficult hematologic malignancy that requires more selective therapeutic strategies. Here, we present the in silico design and comprehensive characterization of a recombinant immunotoxin composed of an anti-CD33 single-chain variable fragment (scFv) fused to the LukS-PV pore-forming toxin. Structural modeling and refinement confirmed correct immunoglobulin-domain folding, preserved LukS-PV architecture, and high stereochemical quality. Physicochemical and mRNA analyses indicated favorable stability, solubility, and strong translational compatibility with

Co-overexpression of CDC27 and PD-L1 defines an ultra-high-risk subgroup of acute myeloid leukemia in an Egyptian cohort.

Acute myeloid leukemia (AML) is a heterogeneous hematologic malignancy characterized by uncontrolled proliferation of leukemic cells. Emerging evidence suggests that dysregulation of cell-cycle regulators may interact with immune checkpoint pathways; however, this relationship remains insufficiently explored in AML. To evaluate the expression of CDC27 and PD-L1 in adult AML patients and assess their clinicopathological and prognostic significance. This case - control study included 80 participants: 40 newly diagnosed adult AML patients and 40 age- and sex-matched healthy controls. Gene express