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Puerperal infectious morbidity: relationship to route of delivery and to antepartum Chlamydia trachomatis infection.

We investigated the relationship of route of delivery and of antepartum Chlamydia trachomatis cervical infection to selected forms of puerperal infectious morbidity, including intrapartum fever, or early (less than 48 hours) postpartum fever not attributable to infections of sites outside the uterus and late (48 hours to 6 weeks) postpartum endometritis. Infectious morbidity occurred in 27 (44%) of 62 women who underwent cesarean section and in 33 (10%) of 329 who underwent vaginal delivery (p less than 0.001). Postsection infectious morbidity was not correlated with C. trachomatis infection a…

Identification of the elementary bodies of Chlamydia trachomatis in the electron microscope by an indirect immunoferritin technique.

An indirect immunoferritin (IIF) technique is described for recognizing the elementary bodies (EB) of Chlamydia trachomatis in unsectioned preparations. Both the EB of a genital strain of C. trachomatis grown in irradiated McCoy cells and EB in clinical specimens obtained from patients attending a venereal disease clinic were identified by the IIF test in the electron microscope. Cell culture-grown EB were detected by ferritin staining for up to 4 weeks after the organisms had lost their infectivity for tissue cultures. The IIF test was of comparable sensitivity to isolation methods in detecti…

Worldwide prevalence of Chlamydia trachomatis in infertile women: A systematic review and meta-analysis.

INTRODUCTION: Chlamydia trachomatis (CT) infection is a prevalent sexually transmitted disease (STD). It seems that CT infections ascending to the uterus can result in serious reproductive health complications, including infertility. Therefore, this systematic review and meta-analysis was conducted to assess the worldwide prevalence of CT among infertile women. METHODS: A systematic search was conducted across three major databases, Medline (via PubMed), Web of Science, and Scopus, to identify eligible studies between 2010 and July 2025. Keywords such as "Chlamydia trachomatis," "infertility,…

The global prevalence of Chlamydia trachomatis genital infection in pregnant women: a meta-analysis.

BACKGROUND: Chlamydia trachomatis genital infection is one of the most common sexually transmitted bacterial infections with severe detrimental effects on pregnant women and fetuses. CTGI increases the risk of ectopic pregnancy, exogenous fetal infection, and respiratory complications such as bronchitis and pneumonia. According to the different published reports, this systematic review and meta-analysis study aimed to evaluate the global prevalence of CTGI in pregnant women. METHODS: Initial searching was applied using valid databases of PubMed, ScienceDirect, Embase, WoS, Scopus, and Google …

Structural Optimization of Covalent Inhibitors for Deubiquitinase ChlaDUB1 of Chlamydia trachomatis as Antibiotic Agents.

The cysteine protease ChlaDUB1 has proven to be a promising new target for antichlamydial therapy. This deubiquitinase manipulates protein homeostasis of the infected human host cell, concealing the chlamydial infection. In this study, we optimized a previously identified scaffold of covalently acting ChlaDUB1 inhibitors using a combination of docking, synthesis and in vitro enzymatic screening. This led to a reduction of the inhibitor size while simultaneously improving activity at ChlaDUB1 to 1 μM and enhancing the rate of target inhibition. We identified a hitherto unobserved inhibition mec

FtsK is critical for the assembly of the unique divisome complex of the FtsZ-less Chlamydia trachomatis.

Chlamydia trachomatis serovar L2 (Ct), an obligate intracellular bacterium that does not encode FtsZ, divides by a polarized budding process. In the absence of FtsZ, we show that FtsK, a chromosomal translocase, is critical for divisome assembly in Ct. Chlamydial FtsK forms discrete foci at the septum and at the base of the progenitor mother cell, and our data indicate that FtsK foci at the base of the mother cell mark the location of nascent divisome complexes that form at the site where a daughter cell will emerge in the next round of division. The divisome in Ct has a hybrid composition, co…

Для поглощения CTP хламидией трахоматисом требуется преобразование цитидина в CTP в зависимость от UCK2.

Хламидия трахоматис, обязуемая внутриклеточная бактерия, превращается в вакулярное отделение, называемое включением. бактерии импортируют нуклеосайд трифосфаты (НТФ), присутствующие в люмене для включения. Остается неясным, попадают ли нуклеозиды в люмен в их родной форме или в качестве фосфорилатированных нуклеотидов. Используя химию щелчка в сочетании с микроскопией флуоресцентной активности, мы представляем доказательства того, что цитидин требует фосфорилирования с помощью уридина-цитидиновой киназы 2 (UKK2) до его включения в бактериальные нуклеиновые кислоты. Эти выводы подтверждают гипо

Совпадение в аннотации оригинала
Chlamydia trachomatis , an obligate intracellular bacterium, develops into a vacuolar compartment called the inclusion. The bacteria import nucleoside triphosphates (NTPs) present in the inclusion lumen. It remains unclear whether nucleosides enter the inclusion lumen in their native form or as phosphorylated nucleotides. Using click chemistry coupled with f…

Chlamydia trachomatis infection and risk of ovarian cancer: a systematic review and meta-analysis.

Chlamydia trachomatis infection has been implicated as a potential risk factor for ovarian cancer (OC), but evidence remains inconclusive. This study aims to assess the association between C. trachomatis infection and OC risk using a systematic review and meta-analysis. A comprehensive literature search was conducted in PubMed, Embase, Scopus, Web of Science, and SciELO from their inception to October 2024. Observational studies examining the association between C. trachomatis infection and OC risk were included. Pooled odds ratios (ORs) were calculated using random-effects models. Subgroup an…

Assessment of Chlamydia trachomatis testing in Sweden 2016-2023 and the incidence of associated complications.

BACKGROUND: This study aimed to assess chlamydia diagnostics in different clinic types, including internet-based self-sampling (IBSS). Furthermore, we investigated the incidence of chlamydia-associated complications. METHODS: Data on Chlamydia trachomatis (CT) testing were retrieved from six healthcare regions in the years 2016-2023 across different categories of testing facilities. National data on CT diagnostics and number of PID, ectopic pregnancy and infertility cases were obtained from Swedish health authorities. RESULTS: The number of CT cases detected through IBSS increased by 85% fro…

Protein tyrosine phosphatase PTP1B is a positive regulator of the intracellular development of Chlamydia trachomatis.

The intracellular survival and replication of Chlamydia trachomatis rely on the precise manipulation of host signaling pathways. Host kinases are instrumental in the modulation of host signaling during C. trachomatis infection. However, the potential contribution of host phosphatases to chlamydial pathogenesis remains poorly understood. Here, we identified the host tyrosine phosphatase PTP1B as a positive regulator of C. trachomatis intracellular development. Gain-of-function approaches revealed that PTP1B promotes inclusion development and increases the production of infectious elementary bod…

Azithromycin alters the microbiome composition, function and resistome in women with Chlamydia trachomatis infections.

Antibiotics disrupt mucosal microbial communities, yet the effects on microbiomes infected with Chlamydia trachomatis (Ct) remain poorly understood. Some data exist on vaginal microbiomes, but none exist for the endocervix or rectum that are primary sites of infection. We applied metagenomic shotgun sequencing to vaginal, endocervical and rectal samples collected longitudinally from women who cleared their infection post-treatment (n = 10), had persistent infection (n = 11), or remained uninfected (n = 18) to evaluate azithromycin-induced changes in microbial composition, function, and the res…

Prevalence of Chlamydia trachomatis and Neisseria gonorrhoeae in women of the Czech Republic armed forces.

BACKGROUND: Sexually transmitted infections (STIs) are a serious health problem with global impact, more prominently in women. Their prevalence is increasing, and the preventive measure options are not being sufficiently innovated. The military environment is historically characterized by a higher incidence of STIs. A prospective cross-sectional study was conducted in order to analyze prevalence of common STIs in women of the Czech Republic armed forces. MATERIAL AND METHODS: Chlamydia trachomatis (C. trachomatis) and Neisseria gonorrhoeae (N. gonorrhoeae) detection was performed in 231 women…

A suitable and efficient optimization system for the culture of Chlamydia trachomatis in adult inclusion conjunctivitis.

The prevalence of Chlamydia trachomatis infection in the genitourinary tract is increasing, with an annual rise of 9 million cases. Individuals afflicted with these infections are at a heightened risk of developing adult inclusive conjunctivitis (AIC), which is commonly recognized as the ocular manifestation of this sexually transmitted infection. Despite its significant clinical implications, the lack of distinctive symptoms and the overlap with other ocular conditions often lead to underdiagnosis or misdiagnosis of AIC associated with C. trachomatis infection. Here, we established six distin…

The N-terminus of the Chlamydia trachomatis effector Tarp engages the host Hippo pathway.

Chlamydia trachomatis is an obligate, intracellular Gram-negative bacteria and the leading bacterial STI in the United States. Chlamydia's developmental cycle involves host cell entry, replication within a parasitophorous vacuole called an inclusion, and induction of host cell lysis to release new infectious particles. During development, Chlamydia manipulates the host cell biology using various secreted bacterial effectors. The early effector Tarp is important for Chlamydia entry via its well-characterized C-terminal region which can polymerize and bundle F-actin. In contrast, not much is kno…

Prevalence of Ureaplasma urealyticum, Mycoplasma hominis and Chlamydia trachomatis in symptomatic and asymptomatic patients.

The purpose of the present study was to assess the prevalence of Ureaplasma urealyticum (U. urealyticum), Mycoplasma hominis (M. hominis) and Chlamydia trachomatis (C. trachomatis) in a Romanian population considering the presence or absence of genital symptoms. Urethral and vaginal samples were collected from patients presenting at 'Ponderas' Academic Hospital (Bucharest, Romania) from January 2021 to December 2021. A total of 266 samples were obtained from two groups of patients: Symptomatic subjects with urethritis, prostatitis, vaginitis or both urethritis and prostatitis (n=59; 22%), and …

Dynamin-dependent entry of Chlamydia trachomatis is sequentially regulated by the effectors TarP and TmeA.

Chlamydia invasion of epithelial cells is a pathogen-driven process involving two functionally distinct effectors - TarP and TmeA. They collaborate to promote robust actin dynamics at sites of entry. Here, we extend studies on the molecular mechanism of invasion by implicating the host GTPase dynamin 2 (Dyn2) in the completion of pathogen uptake. Importantly, Dyn2 function is modulated by TarP and TmeA at the levels of recruitment and activation through oligomerization, respectively. TarP-dependent recruitment requires phosphatidylinositol 3-kinase and the small GTPase Rac1, while TmeA has a p

Evaluation of Chlamydia trachomatis screening from the perspective of health economics: a systematic review.

BACKGROUND: Most Chlamydia trachomatis (CT) infections are asymptomatic. The infection can persist and lead to severe sequelae. Therefore, screening for CT can primarily prevent serious sequelae. AIM: To systematically evaluate CT screening from the perspective of health economics, summarize previous findings from different target populations, and make practical recommendations for developing local CT screening strategies. METHODS: PubMed, Web of Science, Embase, Cochran Library, and National Health Service Economic Evaluation Database (Ovid) were searched from January 1, 2000, to March 4, 2…