Результаты поиска

[The investigation of the mechanism underlying the impairment of the intestinal mucous barrier in severe hypertriglyceridemia acute pancreatitis].

OBJECTIVE: To investigate the structural and functional alterations of intestinal goblet cells and the mucus barrier in rats with hypertrigl … ssay (ELISA) was performed to measure secretory immunoglobulin A (sIgA) level in intestinal mucosa. Alcian blue/periodic acid-Schiff (AB/PAS) staining was performed to evaluate mucus layer structure and to measure mucus layer thickness and goblet cell number. Double immunofluorescence staining was performed to detect NOD-like receptor protein 6 (NLRP6) and mu …

Disrupted tRNA modification leads to intestinal mitochondrial dysfunction and microbial dysbiosis.

BACKGROUND AND AIMS: Transfer RNA (tRNA) modifications determine translation fidelity and efficiency. It occurs through the action of specif … thy control. Qtrt1 -/- mice had significant reduction of Q-associated bacteria, e.g., Bacteroides . Alcian Blue and Mucin-2 staining revealed mucosal barrier damage and disrupted homeostasis, with reduced colonic cell proliferation. Intestinal tight junction integrity was impaired in QTRT1-KO mice, as evidenced by reduced ZO-1 and increased Claudin-10 express …

[Effect of electroacupuncture on necroptosis RIPK1/RIPK3/MLKL signaling pathway in rats with gastric precancerous lesions].

OBJECTIVES: To observe the effect of electroacupuncture (EA) on gastric mucosal injury and necroptosis receptor-interacting protein kinase ( … opathological changes of the gastric tissues were observed by hematoxylin-eosin (H.E.) staining and alcian blue-periodic acid schiff (AB-PAS) staining, separately. The ELISA was used to detect the contents of tumor necrosis factor alpha (TNF-α), interleukin 1 beta (IL-1β) and transforming growth factor beta 1 (TGF-β1) in the serum. Immunohistochemistry (IHC) …

Roux-en-Y reconstruction alleviates radical gastrectomy-induced colitis via down-regulation of the butyrate/NLRP3 signaling pathway.

BackgroundDifferent methods for digestive tract reconstruction have a complex impact on the nutritional status of gastric cancer (GC) patients after radical gastrectomy. Previous studies reported that Roux-en-Y (R-Y) reconstruction resulted in obvious weight reduction and improvement in type 2 diabetes in obese patients. We investigated the relationship between R-Y reconstruction, gut microbiota, and the NLRP3 inflammasome in GC patients with poor basic nutrition.MethodsChanges in the gut microbiota after radical gastrectomy accomplished by different methods of digestive tract reconstruction w

Совпадение в полном тексте источника
… The entire colon was stained with hematoxylin and eosin (H&E) and Alcian blue. Colon inflammation, area, hyperplasia, and dysplasia of the mucosa and submucosa were observed by H&E staining. Acidic mucus-containing goblet cells were identified by Alcian blue staining. … According to the results of H&E and Alcian blue staining and the histological score, butyrate eased intestinal inflammation to some extent, which may have contributed to the better nutrient levels in the mice in the butyrate group ( Fig. 6 G and H). …

[Effect of Different Doses of Herbal Cake-partitioned Moxibustion on Histopathological Changes of Colon Tissue in Ulcerative Colitis Rats].

OBJECTIVE: To observe the effect of different-doses of herbal cake-partitioned moxibustion (Moxi) on histopathological changes of the damage … id-schiff (PAS) staining, and those of the sulfated mucus content detected after high iron dia-mine-alcian blue (HID-AB) staining. RESULTS: Compared with the normal group, rats in the model group presented loose stool, or with pus and blood, and slowly increased body weight (P<0.01), obvious congestion of colon with ulcer spots or continuous superficial ulce …

Loss of EGFR activity in skeletal stem/progenitor cells is associated with impaired fracture healing in aged mice.

The cellular and molecular events responsible for fracture healing becoming delayed with aging remain unclear. Epidermal growth factor receptor (EGFR) signaling has been reported to play a critical role in bone regeneration. However, knowledge of its specific function in skeletal stem cells during aging-induced fracture delayed union remains scant. In the present study, we first demonstrated that EGFR activity in skeletal stem/progenitor cells decreased as mice aged and thus resulted in delayed fracture healing. To further investigate whether the EGFR signaling can be targeted as a potential t

Совпадение в полном тексте источника
… (G) Alcian blue staining of the WT , HBEGF Over Prx1 and Egfr iCKO periosteal progenitors after culture in chondrogenic medium for 2 weeks with or without H 2 O 2 . Alcian blue-positive areas were measured using Image J; n = 3. … (G) Alcian blue staining of the WT , HBEGF Over Prx1 and Egfr iCKO periosteal progenitors after culture in chondrogenic medium for 2 weeks with or without H 2 O 2 . Alcian blue-positive areas were measured using Image J; n = 3. …

Scleredema of Buschke: 2 cases with a remarkable response to treatment of the underlying paraprotein.

Аннотация не предоставлена источником. Откройте карточку для перехода к публикации.

Совпадение в полном тексте источника
… Skin biopsies in both cases demonstrated reticular dermal expansion, separation of hyalinized collagen bundles ( Figs 3 and 4 ), and interstitial mucin on Alcian blue staining ( Fig 5 ), confirming scleredema of Buschke. Fig 3 The epidermis is generally unaffected with mild atrophy. … (Alcian blue stain; original magnification:×40.) Over several years, both cases remained refractory to various treatments, including UV-A1, mycophenolate-mofetil, and intravenous immunoglobulin. …

Hyaluronan (HA) interacting proteins RHAMM and hyaluronidase impact prostate cancer cell behavior and invadopodia formation in 3D HA-based hydrogels.

To study the individual functions of hyaluronan interacting proteins in prostate cancer (PCa) motility through connective tissues, we developed a novel three-dimensional (3D) hyaluronic acid (HA) hydrogel assay that provides a flexible, quantifiable, and physiologically relevant alternative to current methods. Invasion in this system reflects the prevalence of HA in connective tissues and its role in the promotion of cancer cell motility and tissue invasion, making the system ideal to study invasion through bone marrow or other HA-rich connective tissues. The bio-compatible cross-linking proce

Совпадение в полном тексте источника
… Laemmli sample buffer and alcian blue were purchased from Biorad (Richmond, CA). … All cells in the series showed HAase activity at similar levels as shown by the clear areas on the Alcian blue stained gel ( Figure 8B ). …

[Pathogenesis of precancerous lesions of gastric cancer and treatment mechanism of Weifuchun Capsules via NF-κB/NLRP3 inflammasome signaling pathway].

This study aims to investigate the pathogenesis of precancerous lesions of gastric cancer(PLGC) and explore the potential molecular mechanis … stopathological changes of the gastric mucosa were observed and scored by hematoxylin-eosin(HE) and alcian blue-periodic acid Sthiff(AB-PAS) staining. The levels of inflammatory cytokines including interleukin(IL)-1β, IL-6, IL-18, tumor necrosis factor-alpha(TNF-α), and IL-10 in the gastric tissue were determined by enzyme-linked immunosorbent assay(ELISA). T …

Cyclic Polypeptide D7 Protects Bone Marrow Mesenchymal Cells and Promotes Chondrogenesis during Osteonecrosis of the Femoral Head via Growth Differentiation Factor 15-Mediated Redox Signaling.

Osteonecrosis of the femoral head (ONFH) is a debilitating disease that is closely associated with the clinical application of high-dose glucocorticoids. Elevated oxidative stress contributes to the pathophysiological changes observed in ONFH. The lack of effective treatments besides surgical intervention highlights the importance of finding novel therapeutics. Our previous studies demonstrated that D7, a cyclic polypeptide, enhances the adhesion, expansion, and proliferation of bone marrow mesenchymal stem cells (BMSCs). Therefore, in this study, we investigated the therapeutic effects of D7

Совпадение в полном тексте источника
… The cells were then stained with Alcian blue. 2.11. Alcian Blue Staining After incubation in chondrogenic inductive medium for 21 days, the cells were fixed in 4% PFA for 15 min. …

The Story of Localized Amyloidosis: Something Old and Something New - A Case Series.

Localized amyloidosis is a rare benign disease characterized by extracellular deposition of misfolded proteins in a specific organ without systemic involvement, which might lead to organ dysfunction. Patients with localized amyloidosis do not develop systemic diseases such as cardiac, renal, hepatic, or nerve involvement. Typically, the localized disease is managed by surgical resection, while the systemic disease is treated with a combination chemotherapy and immunotherapy. However, some patients can have challenging clinical presentations, delaying diagnosis and organ dysfunction, and they m

Совпадение в полном тексте источника
… (Right) Sulfated Alcian blue stain highlights the amyloid deposits in turquoise, confirming the presence of amyloid. No viable malignancy was identified. The image displays histologic sections of a right tibial mass, showcasing the presence of nodular deposits consistent with amyloid. … Hematoxylin and eosin stain reveals pink, amorphous extracellular deposits surrounded by moderate histiocytic reaction, while sulfated Alcian blue stain highlights these deposits in turquoise, confirming the amyloid presence. No viable malignancy was identified in the sample. …

Scleredema in a Patient with AIDS-Related Lipodystrophy Syndrome.

Scleredema is a form of cutaneous mucinosis caused by an increased accumulation of collagen and mucin in the dermis. It is characterized by diffused, nonpitting swelling and induration of the skin. Scleredema diabeticorum is one type of scleredema associated with diabetes mellitus. AIDS-related insulin resistance and lipodystrophy syndrome are a newly emerging entities in HIV-infected patients associated with severe metabolic disturbances and insulin resistance. The long-standing diabetes in these patients may contribute to the development of scleredema diabeticorum. Here, we report the rare o

Совпадение в полном тексте источника
… Deposition of acidic mucopolysaccharide between the collagen bundles was prominent and was confirmed by alcian blue staining ( Figure 2(b) ). The distinctive histological features were consistent with scleredema adultorum of Buschke. …

Exploring The Role of TOP2A in the Intersection of Pathogenic Mechanisms Between Rheumatoid Arthritis and Idiopathic Pulmonary Fibrosis Based on Bioinformatics.

BackgroundRheumatoid arthritis (RA) and idiopathic pulmonary fibrosis (IPF) share a common pathogenic mechanism, but the underlying mechanisms remain ambiguous. Our study aims at exploring the genetic-level pathogenic mechanism of these two diseases.MethodsWe carried out bioinformatics analysis on the GSE55235 and GSE213001 datasets. Machine learning was employed to identify candidate genes, which were further verified using the GSE92592 and GSE89408 datasets, as well as quantitative real-time PCR (qRT-PCR). The expression levels of TOP2A in RA and IPF in vitro models were confirmed using West

Совпадение в полном тексте источника
… Not only that, we also discovered that PluriSIn #2 could alleviate the deposition of the extracellular matrix through high-density culture and Alcian blue staining ( Figure 6C ). … blue staining show the effect of extracellular matrix deposition in A549 with PluriSin #2 (****<0.0001). …

Lysosomal storage diseases.

Lysosomes are cytoplasmic organelles that contain a variety of different hydrolases. A genetic deficiency in the enzymatic activity of one of these hydrolases will lead to the accumulation of the material meant for lysosomal degradation. Examples include glycogen in the case of Pompe disease, glycosaminoglycans in the case of the mucopolysaccharidoses, glycoproteins in the cases of the oligosaccharidoses, and sphingolipids in the cases of Niemann-Pick disease types A and B, Gaucher disease, Tay-Sachs disease, Krabbe disease, and metachromatic leukodystrophy. Sometimes, the lysosomal storage ca

Совпадение в полном тексте источника
… The cytoplasmic inclusions in visceral histiocytes are PAS-positive, Alcian blue-positive, weakly metachromatic, and weakly sudanophilic. The neuronal lipidosis occurs throughout the cortex, brainstem, and spinal cord and also in the myenteric plexus. … blue, PAS, and toluidine blue positive) | + | + | + Spleen Splenomegaly | + | + | + Storage histiocytes | + | + | + Liver Hepatomegaly | + | + | + Alcian blue, PAS, and toluidine blue positive material in hepatocytes | – | + | + Alcian blue, PAS, and toluidine blue positive material in Kupffer cells …

GZMK-expressing CD8+ T cells promote recurrent airway inflammatory diseases.

Inflammatory diseases are often chronic and recurrent, and current treatments do not typically remove underlying disease drivers1. T cells participate in a wide range of inflammatory diseases such as psoriasis2, Crohn's disease3, oesophagitis4 and multiple sclerosis5,6, and clonally expanded antigen-specific T cells may contribute to disease chronicity and recurrence, in part by forming persistent pathogenic memory. Chronic rhinosinusitis and asthma are inflammatory airway diseases that often present as comorbidities7. Chronic rhinosinusitis affects more than 10% of the general population8. Am

Совпадение в полном тексте источника
… d , Representative images of Alcian Blue-Periodic acid Schiff (AB-PAS) staining of lung tissue sections. Scale bar, 100 μm. e , Quantifications of PAS + areas in the airway. f-g , CD8 T cell intrinsic role of GZMK. f , Experimental setup. … The tissue blocks were cryo-sectioned into 10 μm slices, and Alcian Blue-Periodic acid Schiff (AB-PAS) staining was performed using the AB-PAS solution set (Servicebio) according to the manufacturer’s instructions. …

Cystatin M/E Ameliorates Multiple Myeloma-Induced Hyper Osteolytic Bone Resorption.

Multiple myeloma (MM) is a malignancy of terminally differentiated B-cells that is localized primarily in the bone marrow (BM) but also can be present in peripheral blood and tissue/organs [...].

Совпадение в полном тексте источника
… Following 14 days of treatment in a chondrocyte differentiation medium, Alcian blue staining demonstrated that the glycosaminoglycan (GAG)-rich extracellular matrix (ECM) found during chondrogenesis was suppressed by ZA treatment but not rmCst6 ( Figure 4 E). … After 14 days, cells were washed with PBS, fixed with 4% paraformaldehyde (20 min, room temperature), and then stained with Alcian blue staining solution (Sigma Aldrich, catalog # TMS-010-C) for 30 min at room temperature [ 51 ]. …

The paracrine effects of tumor-resident mesenchymal stem/stromal cells in the microenvironment of brain metastases.

BACKGROUND: Tumor-resident mesenchymal stem/stromal cells (MSCs) were identified in several cancers, and were implicated in regulating multiple aspects of tumor progression. However, the role of tumor-resident MSCs in the pathophysiology of brain metastases (BrMs) -the most prevalent type of malignant brain tumor- is currently unknown, and was investigated in this study. METHODS: Tumor-resident MSCs were isolated from fresh human lung-BrM tissues (n=3) and were phenotypically assessed in FFPE lung-BrM tissues (n=11). The paracrine effects of BrM-MSCs on neutrophil granulocytes and endothelial

Совпадение в полном тексте источника
… (B) Representative micrographs of the tri-lineage differentiation of BrM-MSCs’ tri-lineage differentiation assay showing lipid vesicles stained with Sudan III (left panel), cartilage stained with Alcian blue (middle panel), and mineralized bone matrix stained with Alizarin red (right panel). …

Update on cutaneous calciphylaxis.

Calciphylaxis is a devastating disorder with a mortality rate of 80% due to sepsis and organ failure. Hallmarks of this rare disease are arteriolar media calcification, thrombotic cutaneous ischemia, and necrotic ulcerations. Different mechanisms of vascular calcification can lead to calciphylaxis. Early diagnosis by deep cutaneous ulcer biopsy is most important for prognosis. Here, dermatologists play a significant role although treatment usually needs an interdisciplinary approach. Surgical procedures had been the cornerstone of treatment in the past including parathyroidectomy, but recently

Совпадение в полном тексте источника
… a) Hematoxylin-eosin. b) Alcian blue. c) Congo red. d) Von Kossa. When is parathyroidectomy curative in calciphylaxis? a) In any case of calciphylaxis. b) In renal end-stage related calciphylaxis. c) In primary hyperparathyroidism. d) Never. …

Синергический потенциал стволовых клеток костного мозга и miR181-a комбинированная терапия против множественных склерозов.

Множественный склероз (MS) — прогрессивное аутоиммунное заболевание, характеризующееся массовой воспламенительной инфильтрацией, демиелинацией и последующими повреждениями аксона и нейронными повреждениями в центральной нервной системе (КНС). Этиология MS остается неясной, и пока еще нет окончательного графика лечения этого заболевания. Месенхимальные стволовые клетки костного мозга (МСЦ), демонстрирующие функции нейроиммунной модуляции в целях смягчения различных аутоиммунных заболеваний, демонстрируют большой потенциал в лечении MS. Вместе с тем нестабильность иммуноусилительного воздействия

Совпадение в полном тексте источника
… When the chondrosphere reaches a diameter of 1.5 to 2 mm after 21 days, prepare frozen sections and stain them with Alcian Blue 8GX solution. …

Histone Deacetylase Inhibitors as a Therapeutic Strategy to Eliminate Neoplastic "Stromal" Cells from Giant Cell Tumors of Bone.

The neoplastic "stromal" cells in giant cell tumor of bone (GCTB) harbor a mutation in the H3F3A gene, which causes alterations in the epigenome. Current systemic targeted therapies, such as denosumab, do not affect the neoplastic cells, resulting in relapse upon treatment discontinuation. Therefore, this study examined whether targeting the epigenome could eliminate the neoplastic cells from GCTB. We established four novel cell lines of neoplastic "stromal" cells that expressed the H3F3A p.G34W mutation. These cell lines were used to perform an epigenetics compound screen (n = 128), which ide

Совпадение в полном тексте источника
… Subsequently, MCTS were fixed in 4% formaldehyde containing Alcian blue (1:200 dilution) and prepared for paraffin embedding with the Epredia™ Cytoblock™ Cell Block Preparation System. H&E stains were performed using the Leica ST5020-CV5030 Stainer Integrated Workstation. …