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Modular organization of rabies virus phosphoprotein.

A phosphoprotein (P) is found in all viruses of the Mononegavirales order. These proteins form homo-oligomers, fulfil similar roles in the r … ion, that the central domain is the oligomerization domain, and that the C-terminal domain binds to nucleocapsids. Our results suggest a conserved organization of P proteins in the Rhabdoviridae family in concatenated functional domains resembling that of the P proteins in the Paramyxoviridae family.

An outbreak of fatal herpesvirus infection in domestic rabbits in Alaska.

A herpesvirus infection affecting mini Rex and crossbred meat rabbits was identified in a rabbitry in Alaska. Illness affected over half of … vitro. The viral isolate was characterized ultrastructurally as an enveloped virus with icosahedral nucleocapsids 100 nm diameter, consistent with a herpesvirus.

Paget's disease of bone.

The highest incidence of Paget's disease is in the United Kingdom and is lower in other European countries. Worldwide the incidence appears … possible role of paramyxovirus infection in the etiology of Paget's disease. Altered measles virus nucleocapsid transcripts have been detected in bone marrow cell cultures and peripheral blood mononuclear cells from Paget's disease patients. Canine distemper virus may be the etiologic agent, and ownership of a mongrel dog or a dog not vaccinated against canin …

Functional complementation of UL3.5-negative pseudorabies virus by the bovine herpesvirus 1 UL3.5 homolog.

The UL3.5 gene is positionally conserved but highly variable in size and sequence in different members of the Alphaherpesvirinae and is absent from herpes simplex virus genomes. We have shown previously that the pseudorabies virus (PrV) UL3.5 gene encodes a nonstructural protein which is required for secondary envelopment of intracytoplasmic virus particles in the trans-Golgi region. In the absence of UL3.5 protein, naked nucleocapsids accumulate in the cytoplasm, release of infectious virions is drastically reduced, and plaque formation in cell culture is inhibited (W. Fuchs, B. G. Klupp, H. …

The capsid architecture of channel catfish virus, an evolutionarily distant herpesvirus, is largely conserved in the absence of discernible sequence homology with herpes simplex virus.

Although herpesviruses have a wide host range and their genomes vary substantially in size, the nucleocapsid appears to be a conservative element of viral design. The capsid shell is icosahedrally symmetric (T = 16), and 125 nm in diameter and 15nm thick in the case of herpes simplex virus 1 (HSV-1). Channel catfish virus (CCV) has the gross morphology of a herpesvirus, although no relationship to other herpesviruses is evident from the sequences of its proteins. To examine CCV capsid architecture more closely, we have determined its structure by cryoelectron microscopy and three-dimensional i…

Viral superantigen-induced hyporesponsiveness of T cells and polyclonal B cell activation in HIV-1 infection.

The mechanisms of CD4 depletion and hyporesponsiveness during human immunodeficiency virus (HIV) infection are still unknown. Given the ability of superantigens to stimulate a higher number of lymphocytes than conventional antigens, they may play a major role in this process. Recently, a novel superantigen, the rabies virus nucleocapsid (NC), was described in humans. In the present work, we tested the responses of peripheral blood lymphocytes from asymptomatic HIV-infected patients to this superantigen. In contrast to its effect in normal controls, NC failed to expand T cells from HIV-infected…

Intracellular formation of two soluble glycoproteins in BHK cells infected with vesicular stomatitis virus serotype New Jersey.

Infection of BHK 21 cells with VSV serotype New Jersey gave rise to three intracellular viral glycoproteins: the membrane-integrated G prote … embranes when primed with RNA extracted from VSV-infected cells or with newly transcribed mRNA from nucleocapsids in a coupled transcription system. Thus, proteases localized in the lumen of the ER seemed to be not essential for the generation of both soluble glycoproteins.

Sequence analysis of the S RNA of the African arenavirus Mopeia: an unusual secondary structure feature in the intergenic region.

Mopeia virus is an apparently nonpathogenic African arenavirus which can protect animals from subsequent challenge by the closely related Lassa virus. As a step toward understanding these differences in pathogenicity and the means by which Mopeia virus infection can protect against subsequent Lassa virus infection, cDNA clones corresponding to 3419 nucleotides of Mopeia virus S RNA were isolated and sequenced. Two open reading frames, encoding the glycoprotein precursor (GPC) and nucleocapsid (N) proteins, were located in the ambisense arrangement characteristic of the arenaviruses. Comparison…

Обнаружение геномных последовательности вируса кори в мозговой ткани SSPE по цепной реакции полимеразы.

В качестве предварительного шага была изменена цепная реакция полимеразы (PCR) для обнаружения геномных последовательностей RNA путем создания копий этих последовательностей CDNA. Были синтезированы пары олигонуклеотидов, дополняющие последовательности в каждом из пяти основных структурных генов белка вируса кори (белок нуклеокапсид, фосфопротеин, матрицовый протеин, белок синтеза и протеин гемагглютинина). Продукты ПЦР были в предварительном порядке идентифицированы путем визуализации полос соответствующего размера путем пятнаэтидиумного метила после геля электрофореза, и их идентичность была

Совпадение в аннотации оригинала
The polymerase chain reaction (PCR) was modified to detect RNA genomic sequences by generating cDNA copies of these sequences as a preliminary step. Oligonucleotide primer pairs complementary to sequences in each of the five major structural protein genes of the measles virus (nucleocapsid protein, phosphoprotein, matrix protein, fusion protein, and hemagglu…

Visualization of the fate of inactive influenza viruses in Daudi cells by electron microscopy.

The replication of active and inactivated influenza viruses in Daudi lymphoma cells was studied by immunofluorescence and electron microscop … umerous particles. Production of new viral particles was not demonstrated in this study even though nucleocapsids from the original virus were found in the cytoplasm. Thus, it appears that the massive penetration of the viral particles into cells damages the plasma membrane and may be responsible for the oncolytic potential of the heat-inactivated virus on Dau …

Evaluation of the GeneTex U-OK SARS-CoV-2 Neutralizing Antibody Detection Assay: Comparison With GenScript cPass and Viral Neutralization Assays.

The detection of neutralizing antibodies (NAbs) is crucial for evaluating immune protection against viral infections. This study assessed tw … dividuals. No participant had evidence of prior SARS-CoV-2 infection, as confirmed by negative anti-nucleocapsid antibody tests. Compared with the GenScript cPass kit, the GeneTex U-OK kit demonstrated 100% positive agreement (95% CI: 90.4-100.0%) and 100% negative agreement (95% CI: 95.4-100.0%) in detecting NAbs against the wild-type strain, corresponding to …

Epstein-Barr virus-encoded microRNAs: central players in nasopharyngeal and gastric carcinoma pathogenesis.

Epstein-Barr virus (EBV) is a complex human herpesvirus characterized by a protein core, a 162-capsomer nucleocapsid, and a glycoprotein-spiked envelope, which facilitates its transmission through bodily fluids. The virus primarily targets B cells and oropharyngeal epithelial cells, establishing infection through viral gp350/220 binds to the host CD21/CR2 receptor, followed by gp42 interacting with HLA class II molecules to trigger endocytosis. Once infection is established, EBV utilizes two main types of encoded microRNAs to regulate the host environment. The BHRF1 miRNAs are expressed early …

Integrative Multidimensional Profiling of Individuals Recovered from Mild COVID-19 Reveals Immune-Metabolic-Oxidative Network Interactions.

The COVID-19 pandemic underscored the need to better characterize immune and molecular responses following SARS-CoV-2 infection and vaccinat … y induction. Additional associations linked symptom patterns to T cell differentiation states, anti-nucleocapsid responses to systemic inflammation, and anaerobic signatures to DNA damage markers, revealing interconnections between immunometabolism, clinical expression, and genomic stress. Despite the small sample size, these findings offer a preliminary syste …

Effect of two double-stranded RNA viruses on the virulence of the phytopathogenic fungus Fusarium oxysporum.

Fusarium root rot, a persistent soil-borne disease, poses a serious threat to crop production, quality, and ultimately to food security. We … ite dsRNA virus with 2,944 nt, containing two ORFs which encode a protein of 590 aa RdRp and 134 aa nucleocapsid protein, respectively. It belongs to the genus Unirnavirus, family Amalgaviridae. Furthermore, we demonstrated that both FoPV1/3S18 and FoV1 can be successfully transmitted via hyphal anastomosis to a virus-free strain. Co-infection with FoV1 and Fo …

Ultrasensitive Detection of Porcine Epidemic Diarrhea Virus Infections Using Multivalent DNA Nanostructure-Enabled Lateral Flow Assay.

Early, rapid on-site detection of viral pathogens in livestock is critical to prevent disease spread, minimize economic losses, and safeguar … ptamers. The platform demonstrates the limit of detection (LOD) of ∼1.33 ng/mL for recombinant PEDV nucleocapsid protein and ∼278 viral copies per test in pig oral fluid samples, with results obtained within 10 min without requiring nucleic acid extraction or amplification. Validation with animal clinical samples (n = 102) from swine farms demonstrates an accu …

Placentas From SARS-CoV-2 Infection During Pregnancy Exhibit Foci of Oxidative Stress and DNA Damage.

PROBLEM: COVID-19 during pregnancy is linked to increased maternal morbidity and a higher incidence of preterm births (PTBs), yet the underl … controls (n = 10 term, 3 preterm). The placentas were analyzed for SARS-CoV-2 infection (spike and nucleocapsid viral proteins), markers of DNA damage (γH2AX) and oxidative stress (ROS), and senescence (telomere length, cell cycle regulators, and senescence-associated secretory phenotype [SASP]). RESULTS: Although no overall differences in cellular senescenc …

Anti-SARS-CoV-2 Antibodies in Urine of Individuals Vaccinated with Janssen AD26.COV2.S COVID-19 Vaccine.

Urine-based immunoassay is a non-invasive method with demonstrated utility in detecting anti-SARS-CoV-2 antibodies in unvaccinated patients with COVID-19. To evaluate urine's potential for serological surveys in a real-world setting, SARS-CoV-2 serology was performed on urine samples from vaccinated individuals, both with and without prior confirmed COVID-19. (1) Methods: An in-house indirect ELISA was used to measure antibodies against recombinant spike (S) and nucleocapsid (N) proteins of SARS-CoV-2 in urine and paired serum from 149 individuals vaccinated with Janssen AD26.COV2.S, an S prot…

Beyond the Spike Glycoprotein: Mutational Signatures in SARS-CoV-2 Structural Proteins.

Background: The continuous emergence of SARS-CoV-2 variants represents a major public health concern. Next-generation sequencing (NGS) enables genomic surveillance, facilitating the detection and monitoring of mutations that impact viral evolution. Methods: In this study, full-length SARS-CoV-2 genomes were analyzed between February 2022 and March 2024 as part of routine genomic surveillance conducted in Verona, Italy. Mutations in the envelope (E), membrane (M), and nucleocapsid (N) structural proteins were investigated. Only substitutions with a total prevalence of greater than 1% in the stu…

Development of antibodies against severe fever with thrombocytopenia syndrome virus nucleoprotein for diagnosis.

Severe fever with thrombocytopenia syndrome (SFTS) is an emerging viral infectious disease caused by a novel Bandavirus in the family Phenuiviridae. The SFTS virus (SFTSV) is transmitted to various hosts, including humans, through tick bites, leading to high fever, thrombocytopenia, and leukopenia, with a high case fatality rate (up to 30%) due to multiple organ dysfunction. Therefore, early diagnosis is crucial for effective treatment and preventing disease transmission. In this study, we aimed to develop and characterize monoclonal antibodies targeting the SFTSV nucleocapsid protein (NP). We…

In-silico study of approved drugs as potential inhibitors against 3CLpro and other viral proteins of CoVID-19.

The global pandemic, due to the emergence of COVID-19, has created a public health crisis. It has a huge morbidity rate that was never compr … ed against three crucial viral targets: Spike glycoprotein (7LM9), main protease 3CLpro (7JSU), and Nucleocapsid protein (7DE1). Binding affinities were predicted by using molecular docking, and subsequent predictive regression models, Decision Tree Regression (DTR), Gradient Boosting, XGBoost, Extra Trees, KNNR, and MLP, were constructed employing MACCS molec …