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Proteome analysis of urinary biomarkers in a cigarette smoke-induced COPD rat model.

BackgroundChronic obstructive pulmonary disease (COPD) is a chronic inflammatory airway disease caused by inhalation of cigarette smoke (CS) and other harmful gases and particles.MethodsThis study aimed to explore potential urinary biomarkers for CS-induced COPD based on LC-MS/MS analysis.ResultsA total of 340 urinary proteins were identified, of which 79 were significantly changed (30, 31, and 37 at week 2, 4 and 8, respectively). GO annotation of the differential urinary proteins revealed that acute-phase response, response to organic cyclic compounds, complement activation classical pathway

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… The fixed tissues were embedded in paraffin, sectioned at 4 μm and stained with hematoxylin and eosin (HE) and alcian blue-periodic acid-Schiff (AB-PAS) to reveal histopathological lesions. …

Advanced Diagnostic Techniques

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Evaluation of epidemiological and pathological features of symptomatic spinal metastases in Romania - what could we learn from a retrospective study?

Metastases are the most common tumors of the spine. As an important increase in the annual incidence of spinal metastases (SMs) has been observed in the last decade, the aim of this study was to describe the epidemiology and histopathological types of SMs surgically treated in the Neurosurgery Clinics of a Regional Hospital in North-Eastern Romania over a period of five years, in order to define a certain tumor profile that would benefit from an early screening. We retrospectively evaluated 115 adult patients, searching for demographic data (gender and age of the patients), primary tumor chara

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… New histological sections with 3 μm thickness were obtained from a single representative paraffin block to realize histochemical staining, namely Mucicarmine and Alcian Blue stainings, for mucin identification, but also for immunohistochemical (IHC) staining. …

MSC-EV therapy for bone/cartilage diseases.

Mesenchymal stromal cells (MSCs) have been utilized in cell therapy for various diseases. Recent studies have demonstrated that extracellular vesicles (EVs) released by MSCs play an important role in their therapeutic activities. EVs contain a variety of bioactive molecules such as proteins, messenger RNAs (mRNAs), and micro RNAs (miRNAs) and modify the function of the recipient cells by transferring these molecules. Despite the promising potential of EV therapy as a substitute for MSC therapy, there are challenges that need to be addressed for the clinical success of EV therapy. EV quality ha

Advanced Diagnostic Techniques

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SLAMF7 regulates goblet cell mucus production and negatively impacts gut homeostasis and commensalism.

Crosstalk between the intestinal mucosal barrier and the gut microbiota contributes to maintaining intestinal homeostasis. Accumulating evidence suggests that diverse mechanisms are involved in maintaining intestinal homeostasis. Any disturbance in these pathways can compromise gut homeostasis and trigger chronic inflammatory diseases such as inflammatory bowel disease (IBD). However, how host factors regulate the intestinal mucosal barrier and change the gut microbiome has not been well defined. Here, we discovered that disruption of SLAMF7 protects against intestinal inflammation. SLAMF7 def

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… To test this hypothesis, we examined colon tissues using Alcian blue/periodic acid-Schiff (AB/PAS) staining, which stains glycosylated proteins in goblet cell mucins. The mucus layer was assessed in age- and sex-matched WT and slamf7 -/- mice under steady-state conditions. …

Протеиновый ингибитор смягчает белку в мышах Lmx1b с дисфункциональной доменой LIM.

Мутации в коэффициенте транскрипции LMX1B были определены в качестве причины синдрома автосомали-доминантного заболевания гвозди-пареллы. Он проявляется в небольших или отсутствующих пателле и диспластике или в отсутствии пальцев и ногтей, но прогноз пациентов определяется появлением почечных симптомов из-за дисфункциональных подоцитов. Патогенетические механизмы, ведущие к повреждению подоцита, и их связь с конкретными мутациями в гене LMX1B не понимаются, что препятствует разработке конкретных терапевтических стратегий. Здесь мы идентифицируем патогенетический механизм, затрагивающий многих

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… After several washing steps with 95% ethanol, alcian blue staining of cartilage and subsequent alizarin red staining of bones were performed following standard protocols. For μCT analysis, embryos were collected at 18.5 days of gestation and fixed in 95% ethanol as described above. …

Clostridium butyricum alleviates multiple myeloma by remodeling the bone marrow microenvironment and inhibiting PI3K/AKT pathway through the gut‒bone axis.

Emerging evidence reveals a strong connection between the gut microbiota and cancer. However, the exact role of gut microbiota dysbiosis in multiple myeloma (MM) is poorly understood, and the therapeutic potential of microbiota-targeted interventions represents a promising strategy that demands urgent mechanistic and translational investigation. First, we conducted a comprehensive microbiome-metabolite analysis between MM patients and healthy individuals. The result revealed a marked compositional difference characterized by reduced abundances of butyrate-producing bacteria and diminished buty

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… AB-PAS: Alcian blue-periodic acid Schiff; IL-1β: interleukin-1β; TNF-α: tumor necrosis factor-α. …

Bone Phenotyping Approaches in Human, Mice and Zebrafish - Expert Overview of the EU Cost Action GEMSTONE ("GEnomics of MusculoSkeletal traits TranslatiOnal NEtwork").

A synoptic overview of scientific methods applied in bone and associated research fields across species has yet to be published. Experts from the EU Cost Action GEMSTONE ("GEnomics of MusculoSkeletal Traits translational Network") Working Group 2 present an overview of the routine techniques as well as clinical and research approaches employed to characterize bone phenotypes in humans and selected animal models (mice and zebrafish) of health and disease. The goal is consolidation of knowledge and a map for future research. This expert paper provides a comprehensive overview of state-of-the-art

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… Cartilage is frequently visualized using Alcian Blue ( 199 – 201 ). Moreover, the use of transgenic lines also allows in vivo assessment of specific cell types, including osteoblasts ( 202 , 203 ), osteocytes ( 203 ) and osteoclasts ( 204 , 205 ). …

Резюме

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34th Европейский конгресс патологии - резюме.

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Integumentary System

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… Mucin is more easily demonstrated with stains for glycosaminoglycans, such as Alcian blue and colloidal iron. Mucinous degeneration may be seen as a focal process in numerous inflammatory, neoplastic, and developmental dermatoses. …

Обязательный экзамен по патологии: лишний или необходимый?

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Резюме: 32 Второй Конгресс ЭСП и Международный конгресс XXXIII МПД.

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BREAST

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Musculoskeletal Organs-on-Chips: An Emerging Platform for Studying the Nanotechnology-Biology Interface.

Nanotechnology-based approaches are promising for the treatment of musculoskeletal (MSK) disorders, which present significant clinical burdens and challenges, but their clinical translation requires a deep understanding of the complex interplay between nanotechnology and MSK biology. Organ-on-a-chip (OoC) systems have emerged as an innovative and versatile microphysiological platform to replicate the dynamics of tissue microenvironment for studying nanotechnology-biology interactions. This review first covers recent advances and applications of MSK OoCs and their ability to mimic the biophysic

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… G) Alcian blue staining of tendons at the eighth postoperative week in different groups and semiquantitative analysis. H) Immunofluorescence staining of collagen type II and aggrecan and semiquantitative analysis of sections from different groups at week 8 postoperatively ( n = 4 rats per group). …

Устные бесплатные бумажные сессии.

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Резюме: 31 Европейский конгресс патологии.

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33rd Европейский конгресс патологии - резюме.

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Резюме ЭСП 2013.

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