Результаты поиска

[Determination of 12 halogenated organic pollutants in edible fish by ultra performance liquid chromatography-high resolution mass spectrometry combined with ultrasound-assisted extraction and gel permeation chromatography purification].

Halogenated organic pollutants (HOPs) have attracted considerable attention owing to their persistence, bioaccumulation, and toxicity. The development of methods to detect HOPs in fish is challenging owing to the compositional complexity of fish matrices, which contain high levels of lipids and relatively low concentrations of HOPs. In addition, the lipophilicity of most HOPs renders their extraction difficult. Moreover, the simultaneous determination of multiple HOPs to achieve the high-throughput screening of these analytes is complex. In this study, a reliable and efficient pretreatment met

Examining the Pre- and Post-percutaneous Coronary Intervention Blood Pressure Variability Using Ambulatory Blood Pressure Monitoring in Patients With Stable and Unstable Coronary Artery Disease.

Introduction The World Health Organization has drawn attention to the fact that coronary artery disease (CAD) is our modern "epidemic." Nowadays, sudden death during sleep has become prevalent due to a lack of oxygen supply to the heart. CAD causes more deaths and disabilities and incurs greater economic costs than any other illness in the developed world. The prevalence of cardiovascular disorders and heart disease is on the rise in India. Hypertension is one of the leading risk factors for all cardiovascular diseases. This study aims to compare blood pressure variability before and after per

Proliferating tumor cells mimick glucose metabolism of mature human erythrocytes.

Mature human erythrocytes are dependent on anerobic glycolysis, i.e. catabolism (oxidation) of one glucose molecule to produce two ATP and two lactate molecules. Proliferating tumor cells mimick mature human erythrocytes to glycolytically generate two ATP molecules. They deliberately avoid or switch off their respiration, i.e. tricarboxylic acid (TCA) cycle and oxidative phosphorylation (OXPHOS) machinery and consequently dispense with the production of additional 36 ATP molecules from one glucose molecule. This phenomenon is named aerobic glycolysis or Warburg effect. The present review deals

[Simultaneous determination of 15 bisphenols in prepared dishes by soild phase extraction purification-ultra performance liquid chromatography-tandem mass spectrometry].

Bisphenols （BPs）， which include bisphenol A and its analogs （such as bisphenol S， bisphenol AF， and bisphenol B）， are chemical substances that are synthesized artificially. BPs are used in epoxy resins and polycarbonate plastics and are widely found in food packaging and beverage containers. BPs are endocrine disruptors； hence， they can disrupt the natural hormonal activities of the human body， impact the neurological development of children， and affect intestinal microbial communities in the body， leading to obesity. Consequently， BPs pose threats to human health. The prepared dishes are usua

The impact of vasectomy on the seminal microbiome: possible implications and source of microbes.

STUDY QUESTION: Does vasectomy alter the seminal microbiome, and what proportions of seminal microorganisms originate from the urinary and upper reproductive tract? SUMMARY ANSWER: Vasectomy is associated with modest shifts in the seminal microbial community structure, where inter-individual variability prevails, and the semen shares over 60% of bacterial communities with urine, suggesting an influence of the urinary tract and upstream genitourinary compartments on the seminal microenvironment. WHAT IS KNOWN ALREADY: The semen harbours a polymicrobial community whose origin is not fully unde

[Isolation and proteomics analysis of cerebrospinal fluid exosome subtypes].

Exosomes are small extracellular vesicles 30-200 nm in diameter that contain many bioactive macromolecules, including proteins, lipids, and nucleic acids; consequently, they play important roles in many physiological and pathological processes and are classified into various property-dependent subtypes. Research into exosome heterogeneity helps broaden our understanding of the physiological and pathological mechanisms associated with exosomes. Exosomes exist in many human biological fluids, with those derived from cerebrospinal fluid (CSF) regarded as potential disease biomarkers. Despite this

Multidimensional therapeutic advantages of Smilax glabra (Tufuling)-containing formulae in gout: an integrated Systematic Review and network pharmacology-based prediction.

BACKGROUND: Gout, an inflammatory form of arthritis triggered by monosodium urate (MSU) crystal deposition, poses a substantial global health burden with increasing prevalence and younger onset, particularly in China. In traditional Chinese medicine (TCM), dampness-heat accumulation is a predominant pattern associated with gout. Smilax glabra (Tufuling), a medicinal and edible herb with a history of use for detoxification and elimination of dampness, is also known to promote joint mobility. It is widely used for these purposes. This study aimed to systematically evaluate the clinical efficacy

Ферроптоз в множестве миеломы: молекулярные механизмы и терапевтические возможности.

Множественная миелома (ММ) — гематологическая порча, вызванная распространением клональных плазменных клеток, и остается в значительной степени неизлечимой из-за рецидива заболеваний и приобретенной лекарственной устойчивости. Ферроптоз, основанная на железной зависимости форма регулируемой клеточной смертности, характеризующейся чрезмерным липидным пероксидацией, стала потенциальной терапевтической целью в раке и все чаще причастна к биологии MM. В отличие от апоптоза и других классических путей смерти клеток, ферроптоз тесно связан с дисрегулируемым метаболизмом железа, окислительным стрессо

Role of lysophosphatidic acid and its receptors in health and disease: novel therapeutic strategies.

Lysophosphatidic acid (LPA) is an abundant bioactive phospholipid, with multiple functions both in development and in pathological conditions. Here, we review the literature about the differential signaling of LPA through its specific receptors, which makes this lipid a versatile signaling molecule. This differential signaling is important for understanding how this molecule can have such diverse effects during central nervous system development and angiogenesis; and also, how it can act as a powerful mediator of pathological conditions, such as neuropathic pain, neurodegenerative diseases, an

Advances in MALDI Mass Spectrometry Imaging Single Cell and Tissues.

Compared with conventional optical microscopy techniques, mass spectrometry imaging (MSI) or imaging mass spectrometry (IMS) is a powerful, label-free analytical technique, which can sensitively and simultaneously detect, quantify, and map hundreds of biomolecules, such as peptides, proteins, lipid, and other organic compounds in cells and tissues. So far, although several soft ionization techniques, such as desorption electrospray ionization (DESI) and secondary ion mass spectrometry (SIMS) have been used for imaging biomolecules, matrix-assisted laser desorption/ionization (MALDI) is still t

Cancer immunity and immunotherapy beyond COVID-19.

Аннотация не предоставлена источником. Откройте карточку для перехода к публикации.

Длиннокаменистые толстые кислоты как движущие факторы нейроинфламмации в нейродегенерации: механистические связи с липидом пероксидацией, Ферроптозом и митохондриальной дисфункцией.

Фон: Нейродегенеративные заболевания (НИ) рассматриваются главным образом как расстройства, характеризующиеся серьезным иммунометаболическим дисбалансом, характеризующимся текущей нейроинфламмацией и глиальной активацией. Хотя митохондриальная дисфункция и окислительный стресс являются хорошо известными особенностями, метаболические факторы в верхнем течении, связывающие эти патологические процессы, по-прежнему плохо понимаются. Методы: В ходе этого обзора мы изучили недавние доклинические и клинические исследования, в ходе которых были изучены связи между липидным метаболизмом, иммунометаболи

Связь между окисляющим стрессом, митохондриальной дисфункцией, железным метаболизмом и микроглией в множестве склерозов: обзор нарации.

В последние годы в патогенезе множественного склероза особое внимание уделяется митохондриальным процессам, влияющим на начало заболевания. Окисляющий стресс будет одним из последствий митохондриальной дисфункции, и его воздействие на ткани мозга хорошо описано. Микроглия, как макрофаж мозга, имеет важную функцию в удалении нежелательных метаболитов, а также железа, который является усилителем окислительного стресса. Существуют новшества с точки зрения связи между этими процессами, которые в большей степени перенаправляют исследования на сам процесс нейродегенерации, с тем чтобы больше не дела

Перевозка и сдерживание движения sphingosine-1-phosphate Экспортеры SPNS2.

Sphingosine-1-phosphate (S1P) — сигнал лизолипида, имеющий решающее значение для развития сердца, иммунитета и слуха. Соответственно, мутации в переносчике S1P SPNS2 связаны с уменьшением числа белых клеток и нарушениями слуха. СПНС2 также экспортирует S1P-mimicking FTY720-P (Финголимод) и, таким образом, является центральным элементом фармакокинетики этого препарата при лечении множественного склероза. Здесь мы используем комбинацию крио-электронной микроскопии, иммунофлюоресценции, связывания in vitro и в vivo S1P-оценки экспорта, а также молекулярное моделирование динамики для исследования

Mass Spectrometry-Based Proteomics: Analyses Related to Drug-Resistance and Disease Biomarkers.

Mass spectrometry-based proteomics is a key player in research efforts to characterize aberrant epigenetic alterations, including histone post-translational modifications and DNA methylation. Data generated by this approach complements and enrich datasets generated by genomic, epigenetic and transcriptomics approaches. These combined datasets can provide much-needed information on various mechanisms responsible for drug resistance, the discovery and validation of potential biomarkers for different diseases, the identification of signaling pathways, and genes and enzymes to be targeted by futur

Sympathetic nervous system in tumor progression and metabolic regulation: mechanisms and clinical potential.

Tumor progression is characterized by profound metabolic alterations and dynamic interactions within the tumor microenvironment (TME), which enable rapid proliferation, immunoinvasion, and metastasis. The sympathetic nervous system (SNS), which has been best known for its role in stress regulation, has emerged as a critical regulator of tumor metabolism. The SNS influences glucose, lipid and glutamine metabolism in tumor cells and stromal components by releasing neurotransmitters such as norepinephrine (NE), creating a pro-tumor metabolic and immunosuppressive microenvironment. SNS signaling e

Obesity as a Multifactorial Chronic Disease: Molecular Mechanisms, Systemic Impact, and Emerging Digital Interventions.

Obesity is a multifactorial chronic disease resulting from complex genetic, molecular, environmental, and behavioral interactions. Its prevalence rises worldwide, affecting cardiovascular, metabolic, oncological, hepatic, respiratory, and skeletal health. Beyond caloric excess, genetic predisposition, epigenetic modifications, gut microbiota dysbiosis, endocrine-disrupting agents, circadian misalignment, and intergenerational and prenatal influences are critical determinants of obesity risk. Core pathophysiological mechanisms include insulin resistance, dyslipidemia, chronic low-grade inflamma

Перенос дисфункции химического обмена MRI для нейродегенеративных заболеваний: Обновленная информация о клинических и предклинических исследованиях.

Перенос магнитного резонанса через химический обмен с высокой степенью чувствительности и пространственного разрешения представляет собой передовой метод визуализации, позволяющий обнаруживать соединения при низких концентрациях и широко изучается для диагностики злокачественных свойств и инсульта. В последние годы новые возможности для раннего обнаружения и повторного сканирования без ионизирующего излучения открылись в связи с новыми исследованиями передачи магнитных резонансных изображений с целью обнаружения патологических изменений в нейродегенеративных заболеваниях. Этот обзор служит обз

Using an integrated omics approach to uncover the mechanisms underlying chemotherapy-induced peripheral neuropathy (CIPN).

Chemotherapy-induced peripheral neuropathy (CIPN) is a debilitating side effect with limited treatment options. The primary challenge in developing therapies is the lack of identified neurotoxic mechanisms. To address this, an integrated omics approach, combining transcriptomics, proteomics, and metabolomics, is essential to map the biological changes underlying the condition. Here, we show how data from these complementary approaches converge on key mechanisms involved in CIPN through a critical summary of animal and human studies. Current research highlights several key drivers of CIPN, such

Exercise-modulated IL-6 in cancer cachexia: molecular mechanisms and therapeutic potential.

BACKGROUND: Cancer cachexia is a multifactorial metabolic syndrome marked by progressive skeletal muscle wasting, systemic inflammation, reduced quality of life, and poor clinical outcomes. Interleukin-6 (IL-6) is a central mediator in cachexia pathophysiology, driving inflammatory signaling, catabolic metabolism, and muscle atrophy. However, IL-6 also rises transiently during exercise, where it exerts beneficial metabolic and anti-inflammatory effects, highlighting the context-dependent nature of IL-6 signaling. MAIN BODY: This review summarizes current knowledge on the regulation and functio

Совпадение в полном тексте источника
… Beyond promoting lipid mobilization, IL-6 contributes to adipose tissue insulin resistance by upregulating SOCS3. SOCS3 interferes with insulin receptor substrate (IRS) signaling, further locking adipocytes into a catabolic phenotype [ 70 , 71 ]. … In aggregate, these findings position that HIIT may optimally harness the transient, beneficial profile of IL-6, promoting lipid mobilization and glucose utilization without perpetuating its catabolic actions. …