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A probiotic bacterium modulates antitumour γδ T-cell responses in lung cancer.

The link between the intestinal microflora and cancer outcomes has been recognized for over a decade. Several recent studies have demonstrated that the gut microbiome is associated with the efficiency of T-cell checkpoint blockade therapy for cancer, raising interest in strategies to harness this effect via consumption of live microorganisms (probiotics). The probiotic Clostridium butyricum strain MIYAIRI 588 (CBM588) improves response rates and overall survival in patients receiving immune checkpoint inhibitor (ICI) therapy for non-small cell lung cancer and metastatic renal cell carcinoma bu…

Probiotic cell-free supernatants as a strategy against antimicrobial resistance: a systematic review.

Antimicrobial resistance (AMR) is a critical global health threat that may cause up to 10 million deaths annually by 2050, requiring integrated actions within the One Health framework. The misuse of antimicrobials across human, animal, and environmental sectors has intensified the spread of multidrug-resistant bacteria, including Escherichia coli, Staphylococcus aureus, and Klebsiella pneumoniae. In this context, Lactobacillus-derived postbiotics have emerged as eco-friendly alternatives with antimicrobial and antibiofilm properties. A systematic review was conducted to consolidate scientific

Functional metagenomic reconstruction of microbial pathways altered by probiotic supplementation in liver failure.

INTRODUCTION: Liver failure is a severe condition marked by circulatory failure, systemic inflammation, and gut microbial dysbiosis. This dysbiosis worsens liver damage by reducing beneficial metabolites and increasing harmful products. This study investigates the effects of probiotics on gut microbial functional pathways in liver failure. The aim is to link microbial metabolic reprogramming with host biochemical, inflammatory, and gut barrier responses through functional metagenomic reconstruction. METHODS: Acute liver failure was induced in male Wistar rats using D-galactosamine (700 mg/kg)…

Genomic characterization and preclinical evaluation of the candidate probiotic strain Lactococcus cremoris FBMS_5810.

This study presents a comprehensive genomic and preclinical evaluation of Lactococcus cremoris FBMS_5810, establishing its taxonomic identit … romising candidate but also provides a systematic approach for the identification and validation of probiotics, advancing both fundamental understanding and translational applications in molecular microbiology.

LPS-induced endometrial cell-derived exosomes suppress probiotic Lactobacillus growth.

Spontaneous preterm birth (sPTB) has been increasingly associated with alterations in vaginal microbiota. While Lactobacillus spp., which physiologically dominate the cervical microbiota, are considered protective with a lower risk of intra-amniotic infection and chorioamnionitis, other microbes such as Gardnerella vaginalis are associated with an increased risk of sPTB. Although this association is well documented, the host mechanisms that regulate the composition of vaginal microbial communities remain poorly understood. Emerging evidence suggests that host-derived exosomes may play a critic

Evaluation of the local tolerance and systemic safety of a novel intravaginal probiotic product in cows.

Since its discovery, the microbiota has been increasingly recognised for its role in maintaining health and contributing to various disease conditions. In the reproductive tract, microbial populations can significantly influence endometrial health, internal homeostasis, and fertility. The preservation or restoration of a balanced microbiota through appropriate probiotic products may support reproductive health; in addition, candidate probiotics must be demonstrated to be safe for use. The aim of this study was to assess the local tolerance and systemic safety of a novel intravaginal probiotic …

Клинические эффекты пробиотических добавок на метаболический синдром: систематический обзор и мета-анализ.

Пробиотики привлекли к себе большое внимание в силу их способности модифицировать метаболизм питательных веществ или иммуноводство. Цель этого исследования заключается в оценке эффективности биотического дополнения при лечении метаболического синдрома. МЕТОДЫ: В период с момента создания базы данных по июль проводились систематические обыски в библиотеке < < Пубмед > > , < < Web of Science > > , < < Эмбаза > > и < < Кохрейн > > . 2024. СТАТА/СЕ 14.0 Программное обеспечение использовалось для статистического анализа. РЕЗУЛЬТАТЫ: Всего 12 клиническ

Совпадение в аннотации оригинала
BACKGROUND/OBJECTIVES: Probiotics have attracted much attention due to their ability to modulate nutrient metabolism or immunomodulatory. This study aims to evaluate the efficacy of probiotic supplementation in the treatment of metabolic syndrome. METHODS: PubMed, Web of Science, Embase, and Cochrane Library were systematically searched searched for eligibl…

Saccharomyces boulardii as a Probiotic Supplement in Helicobacter pylori Eradication Therapy: A Meta-analysis.

BACKGROUND AND AIMS: Helicobacter pylori (H. pylori) is an obligate pathogen affecting approximately 50% of the world population. With rising antibiotic resistance rates in H. pylori and increasing dropout rates from eradication therapies due to adverse effects, H. pylori treatment is increasingly challenged. Saccharomyces boulardii (S. boulardii), a probiotic yeast, was shown to reduce infection- and therapy-associated adverse events and increase H. pylori eradication rates. Our meta-analysis summarizes the beneficial effects of S. boulardii supplementation on H. pylori eradication and therap

Genomic atlas of Bifidobacterium infantis and B. longum informs infant probiotic design.

Bifidobacterium longum and B. infantis are pioneer colonizers of the neonatal gut and are widely used as probiotics to support infant growth, development, and disease resistance. However, commercial strains derived largely from high-income countries (HICs) may be suboptimal for infants in low- and middle-income countries (LMICs). We assembled a global genomic atlas of more than 4,000 genomes from 48 countries, increasing representation from LMICs by 12- to 17-fold. High-resolution phylogenomic and functional analyses support delineating B. longum and B. infantis as distinct species with diverg…

Unveiling the Probiotic Properties of Lacticaseibacillus paracasei UFTM 2.9 Through Probiogenomic Analysis.

Lactic acid bacteria (LAB) comprise a group of Gram-positive bacteria with biotechnological applications. LAB, including Lacticaseibacillus spp., are recognized as potential probiotics due to their ability to confer benefits to the host. Here we employ probiogenomic and in vitro analyses to characterize the probiotic potential of Lc. paracasei UFTM 2.9, a LAB that previously demonstrated probiotic properties in vitro. The draft genome of Lc. paracasei UFTM 2.9 comprises 127 contigs, totaling 3 216 252 base pairs, with a GC content of 46.20%. The bacteria showed metabolic versatility, growing i…

An engineered probiotic co-expressing dual melanoma epitopes for therapeutic cancer vaccination.

Therapeutic cancer vaccines face challenges in tumor targeting, adjuvant dependency, and inefficient antigen cross-presentation. We engineered a probiotic vaccine using Escherichia coli Nissle 1917 co-expressing MHC-I epitopes from melanoma antigens Pmel17 and TYRP2. The fusion protein, designed via AlphaFold3, incorporating an IgG Fc domain for stability and Listeriolysin O (LLO) for cytosolic delivery, with expression controlled by an L-arabinose-inducible promoter. The strain retained probiotic properties, was efficiently phagocytosed by antigen-presenting cells, and enhanced their maturati

Probiotic and Postbiotic Delivery Systems for the Management of Chronic Wounds: A Review of Emerging Strategies.

Chronic wounds, particularly diabetic foot ulcers (DFUs) and burn wounds (BWs), impact millions of individuals globally and generate substantial healthcare costs, highlighting the urgent need for more effective therapies aimed at controlling infections. Currently, there is growing evidence of beneficial effects of selected bacterial strains, including Lactobacillus plantarum, Lactobacillus reuteri, Lactobacillus rhamnosus, Lactobacillus paracasei, Bifidobacterium longum, and Escherichia coli, as well as fungi from the genus Saccharomyces spp. Each of these species exerts a distinct influence o

Probiotic Human Study Design and Reporting (PRODARE): Recommendations to Improve Research Practice.

The PRObiotic human study Design And REporting recommendations provide a structured framework for designing and reporting human studies with probiotics, addressing challenges related to product viability. Five main study types are identified. Exploratory studies are early-phase, first-in-human investigations designed to evaluate effects or generate hypotheses and require specification of strain, dose, stability, and assessment of acceptance and adverse events. Pilot studies assess the feasibility of protocols and logistics before larger trials, with clearly defined objectives, compliance measu…

A process-free setup for the preparation of probiotic-loaded electrospun mat as a tailored dressing.

Infected burn wounds remain difficult to treat because increasing antibiotic resistance can limit the effectiveness of conventional antimicrobial therapy. Probiotic offers an alternative, but many probiotic dressings require fabrication, encapsulation, or drying procedures that reduce cell viability. This study developed a post-fabrication method for loading probiotics onto electrospun mats and assessed it in a rat model of infected burn wounds, with mupirocin as the control. Poly(lactic acid) (PLA) and poly(lactic-co-glycolic acid) (PLGA) electrospun mats were characterized for fiber morpholo…

Acetic Acid Bacteria: Metabolic Potential, Technological Applications and Emerging Probiotic Functions.

Acetic acid bacteria (AAB, family Acetobacteraceae) are obligate aerobic microorganisms characterized by a highly efficient oxidative metabolism driven by membrane-bound dehydrogenases. Their ability to incompletely oxidize ethanol and various carbohydrates underlies the production of key food-related metabolites, including acetic acid, gluconic acids, and bacterial cellulose. This review summarizes current knowledge on AAB physiology, metabolic pathways, and ecological adaptations, with emphasis on their relevance to food biotechnology and value-added bioprocesses. AAB plays a central role in

Whole-Genomic and Functional Characterization of Lactiplantibacillus plantarum CLPX21 as Potent Probiotic Candidate.

Lactic acid bacteria (LAB) are regarded as promising probiotics with multiple beneficial properties for humans' and animals' health. In this study, ten LAB isolates were screened and Lactiplantibacillus plantarum (L. plantarum) CLPX21 was selected as a candidate strain with its probiotic potential based on in vitro screening of antimicrobial activity and environmental stress tolerance, including its resistance to acidic environments and bile salts. This strain displayed adhesion capacity with an auto-aggregation rate of 47.15% at 24 h, co-aggregation rates above 67% with pathogenic bacteria, a…

Weissella as an emerging probiotic candidate for IBD: current evidence, challenges, and future perspectives".

Inflammatory Bowel Disease (IBD) is a chronic, relapsing inflammatory affliction that causes gut dysbiosis, impaired mucosal integrity and disrupted immune regulation/responses. Conventional therapies, which aim to suppress inflammation or immune flare-ups, are associated with adverse effects and have limited long-term management efficacy. Therefore, the focus has shifted to the use of microbe-mediated therapeutics, such as multi-strain probiotic blends, for alleviating inflammation and lengthening the relapse period. Within this context, Weissella is an emerging and underexplored genus of Lac

Living Probiotic Microneedles Coordinate Antiandrogen and Nitric Oxide Therapy for Androgenetic Alopecia.

Androgenetic alopecia (AGA) is characterized by follicular miniaturization mediated by androgens along with chronic inflammatory and vascular disturbances. These alterations suggest broader dysregulation involving endocrine pathways, immune activity, and vascular function. Current therapies such as finasteride and minoxidil provide limited and often transient improvement because they target only single components of this multifactorial process. In this study, we developed a living probiotic-based microneedle system that integrates nitric oxide-generating Weissella cibaria (WC) with clascoteron

Probiotic Supplementation and its Potential Benefits in Type 2 Diabetes: A Meta-analysis.

INTRODUCTION: This meta-analysis aims to evaluate the efficacy and safety of probiotic supplementation as an adjunct to standard therapy in patients with type 2 diabetes (T2D), with a focus on its effects on plasma glucose, HbA1c, and insulin levels. METHODS: Specific keywords such as "Type 2 Diabetes", "Probiotics", and "Blood Sugar" were employed to identify relevant clinical and randomized controlled trials. A comprehensive search of PubMed, Scopus, and Web of Science, conducted between September 2010 and October 2024, retrieved 48 studies, of which only 9 met the predefined inclusion crit…

Geometry-Mediated Microgel Structure-Mechanics for Intestinal Region-Specific Probiotic Delivery.

The physiological efficacy of functional food ingredients, particularly probiotics is highly dependent on their spatial localization within the intestine. The non-specific delivery of probiotics severely restricts their therapeutic efficacy. Herein, we developed a sustainable, food-grade microgel delivery platform assembled from corncob nanocellulose building blocks with distinct geometries for programmable intestinal probiotic release. Owing to their distinct topological structures and mechanical properties, spherical nanocellulose-based microgels (CNSM) were found to disintegrate in the ileu…