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miR-21 regulates osteogenic and adipogenic differentiation of BMSCs by targeting PTEN.

ObjectiveTo explore the effects and mechanism of miR-21 on the osteogenic/adipogenic differentiation of mouse BMSCs.MethodsThe bilateral ovaries of C57BL/6J mice (n=24) were removed to construct an osteoporosis model. Real-time quantitative polymerase chain reaction (qRT-PCR) was used to detect the expression of miR-21, osteogenic/adipogenic genes, and PTEN. ALP and ARS and ORO staining were used to detect the formation of calcium nodules and lipid droplets in BMSCs. Western blot was used to detect the expression of PTEN.ResultsmiR-21 was significantly down-regulated in osteoporotic mice. The

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… When the cell confluence reached about 90%, pancreatin (Beyotime, China) was applied for digestion, and cells underwent three passages were used for subsequent experiments. Osteogenic differentiation of BMSCs Mouse BMSCs after three passages were inoculated into a 6-well plate or a 24-well plate. …

ISEV2019 Abstract Book

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Совпадение в полном тексте источника
… pancreatin [ pan′kre- -tin ] a substance from the pancreas of the pig or ox containing digestive enzymes, principally amylase, protease, and lipase; used in the treatment of pancreatic exocrine insufficiency. …