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Rapid visual detection of Mycoplasma gallisepticum by combining recombinase-aided amplification with lateral-flow dipstick assay.

Mycoplasma gallisepticum (MG) is a significant avian pathogen that adversely affects chickens and turkeys globally and is classified as a no … within 5 minutes. The method showed no cross-reactivity with Mycoplasma synoviae, Mycoplasma bovis, Pasteurella multocida, Mannheimia haemolytica, Staphylococcus aureus, Salmonella spp., Escherichia coli, Listeria monocytogenes, Enterococcus faecium, or Enterococcus faecalis. Furthermore, the RAA-LFD method exhibited high sensitivity and good reproducibility, with a de …

The first detection of swine orthopneumovirus in a pig farm in Sweden: a case report.

BACKGROUND: Respiratory diseases are globally a major challenge in today's pig production. Despite the efforts to manage the disease, the nu … e orthopneumovirus. In addition, each sample was also PCR-positive for Mesomycoplasma hyorhinis and Pasteurella multocida, and 2 samples were PCR-positive for Actinobacillus pleuropneumoniae, indicating a polymicrobial respiratory infection. CONCLUSIONS: This report emphasises the importance of ongoing efforts to identify emerging pathogens and determine their clinica …

Development of a multiplex PCR assay for detection of Riemerella anatipestifer serotype 1 and serotype 2 strains.

Riemerella anatipestifer infection is one of the main infectious diseases that threaten the poultry industry at present. Serotypes 1 and 2 a … on band was shown for other species of bacterial pathogens, including Escherichia coli, Salmonella, Pasteurella multocida, Mycoplasma gallisepticum and Mycoplasma synoviae. The sensitivity test showed a detection limit of 102 CFU of the multiplex PCR assay. Furthermore, a total of 60 R. anatipestifer clinical isolates were tested for identification of the serotypes, an …

Metagenomic sequencing sheds light on microbes putatively associated with pneumonia-related fatalities of white-tailed deer (Odocoileus virginianus).

With emerging infectious disease outbreaks in human, domestic and wild animal populations on the rise, improvements in pathogen characteriza … with pneumonia and/or other diseases in ruminants (Mycoplasma ovipneumoniae, Trueperella pyogenes, Pasteurella multocida, Anaplasma phagocytophilum, Fusobacterium necrophorum); however, these species did not substantially differ between case and control sample groups. On average, we detected a larger number of bacterial species in case samples than controls, supportin …

Retrospective analysis of antimicrobial resistance in bacterial pathogens from pet rabbits in Hong Kong, 2019-2022.

Antimicrobial resistance (AMR) is a growing threat to human and animal health. Data are limited on the prevalence of resistant bacteria in p … ed gram-negative bacteria were Pseudomonas aeruginosa (18.1%), followed by Escherichia coli (8.3%), Pasteurella multocida (6.9%), and Klebsiella pneumoniae (4.2%). Approximately 83% of the isolates had acquired resistance to at least one antimicrobial agent, and 49.4% were multidrug-resistant. The isolated bacteria had high levels of resistance to penicillin (69.8%), c …

Field Trial of Antibody Response To Inactivated Bacterial Vaccine in Young Holstein Calves: Influence of Animal Health Status.

INTRODUCTION: Bovine respiratory disease (BRD) is one of the primary causes of death in young calves. Vaccination against infection by the c … immunisations with an inactivated vaccine against the bacterial pneumonia agents Histophilus somni, Pasteurella multocida and Mannheimia haemolytica. At each immunisation, the body weight and height at the withers were measured and the body mass index (BMI) was calculated. Blood was sampled immediately before immunisation and 3 weeks following the booster. The calves w …

Tracheal microbial populations in horses with moderate asthma.

BACKGROUND: There are limited data on potential dysbiosis of the airway microbiota in horses with asthma. HYPOTHESIS/OBJECTIVES: We hypothe … spective case-control study. Aerobic culture was performed on tracheal aspirates, and streptococci, Pasteurella multocida, Chlamydophila spp., Mycoplasma spp., as well as 16S (bacterial) and 18S (fungal) rRNA subunits were quantified by qPCR. RESULTS: Potential pathogens such as Streptococcus spp., Actinobacillus spp., and Pasteurellaceae were isolated from 8, 5, and …

Development of a colloidal gold immunochromatographic strip for rapid detection of Riemerella anatipestifer in ducks.

Riemerella anatipestifer is one of the major bacterial pathogens of ducks and causes significant economic losses in poultry agriculture. Usu … anatipestifer strains and showed no cross-reaction with Escherichia coli, Salmonella enterica, and Pasteurella multocida strains. The sensitivity of the strip for detecting R. anatipestifer was 1.0 × 106 colony forming unit. The strips remained stable for up to 8 mo at 4°C, and the detection can be completed within 15 min. The strip can detect R. anatipestifer in hear …

A mathematical model of dielectrophoretic data to connect measurements with cell properties.

Dielectrophoresis (DEP) brings about the high-resolution separations of cells and other bioparticles arising from very subtle differences in … , and surface charge density for bacteria including Streptococcus mutans, Rhodococcus erythropolis, Pasteurella multocida, Escherichia coli, and Staphylococcus aureus are considered. While the many properties of a cell collapse into one or two features of data, for a well-vetted system the model can indicate the extent of dissimilarity. The influence of individual prop …

Antibacterial activity against porcine respiratory bacterial pathogens and in vitro biocompatibility of essential oils.

Bacterial respiratory infections affecting pigs such as pneumonia, pleuropneumonia, and pleurisy, are a major health concern in the swine in … acillus pleuropneumoniae, Actinobacillus suis, Bordetella bronchiseptica, Haemophilus parasuis, and Pasteurella multocida, with minimum inhibitory concentrations and minimum bactericidal concentrations ranging from 0.01 to 0.156% (v/v). The main component found in cinnamon, thyme, and winter savory oils were cinnamaldehyde, thymol, and carvacrol, respectively. Treating …

Дезинфицирующие соинфекции дыхательных путей: широкополосный анализ птичьих респираторных патогенов на нанофлюидовой платформе ПЦР.

Дыхательные синдромы (РС) относятся к числу наиболее значительных патологических заболеваний у съедобных птиц и вызваны сложными коакциями патогенов и экологическими факторами. В птице с низким патогенным птичьим гриппом A вирусы, метапнеумовирусы, инфекционный бронхит, инфекционный вирус ларингтрахеит, Mycoplasma spp. Escherichia coli и/или Ornithobacterium noztracheale в индейках рассматриваются в качестве основных соинфекционных агентов РС. Аспергиллус sp., пастерелла мультоцида, авибактериум парагаллинарум или хламидиа пситтачи также могут быть вовлечены в вспышки дыхательных путей. Новато

Совпадение в аннотации оригинала
Respiratory syndromes (RS) are among the most significant pathological conditions in edible birds and are caused by complex coactions of pat … terium rhinotracheale in turkeys are considered as key co-infectious agents of RS. Aspergillus sp., Pasteurella multocida, Avibacterium paragallinarum or Chlamydia psittaci may also be involved in respiratory outbreaks. An innovative quantitative PCR method, based on a nanofluidic technology, has the ability to screen up to 96 samples with 96 pathogen-specific PCR prim …

Fusobacterium necrophorum in North American Bighorn Sheep ( Ovis canadensis ) Pneumonia.

Fusobacterium necrophorum has been detected in pneumonic bighorn sheep (BHS; Ovis canadensis ) lungs, in addition to the aerobic respiratory pathogens Mannheimia haemolytica , Bibersteinia trehalosi , Pasteurella multocida , and Mycoplasma ovipneumoniae . Similar to M. haemolytica , F. necrophorum produces a leukotoxin. Leukotoxin-induced lysis and degranulation of polymorphonuclear leukocytes (PMNs) and macrophages are responsible for acute inflammation and lung tissue damage characteristic of M. haemolytica -caused pneumonia. As one approach in elucidating the role of F. necrophorum in BHS p…

The nasopharyngeal microbiota of feedlot cattle that develop bovine respiratory disease.

Bovine respiratory disease is the major cause of morbidity and mortality in feedlot cattle. The objective of this study was to compare the n … had 16S rRNA gene sequences representative of the BRD-associated bacteria Mannheimia haemolytica or Pasteurella multocida, although 3/5 healthy cattle were also positive for M. haemolytica at this time point. The results also indicate that the bovine nasopharyngeal microbiota is relatively unstable during the first 60 days in the feedlot.

Resistance to selected beta-lactam antibiotics.

Susceptibility in vitro and trends in resistance to antimicrobials were determined by a dilution micromethod in a group of Actinobacillus pleuropneumoniae, Pasteurella multocida, Mannheimia haemolytica and Escherichia coli isolates from clinical cases of cattle and swine diseases in the Czech Republic from 2007 to 2011. A high susceptibility of pig and cattle respiratory pathogens to antimicrobials was found, with the exception of the moderate prevalence of M. haemolytica resistance to ampicillin. In contrast to respiratory pathogens, low susceptibility of E. coli of pig and cattle isolates to…

Bacteria isolated from dugongs (Dugong dugon) submitted for postmortem examination in Queensland, Australia, 2000-2011.

Microbial infection may contribute to disease in a significant proportion of marine mammal mortalities, but little is known about infectious … ated with disease. Mixed infections containing Aeromonas spp. and Vibrio spp.; Morganella morganii, Pasteurella multocida, and Serratia marcescens; and Actinomyces spp. and Peptostreptococcus spp. were associated with pneumonia or pleuritis, and Enterococcus faecalis was associated with a multisystemic infection in a neonate. Clostridium spp. was cultured from two anim …

Microbiological identification and analysis of swine tonsils collected from carcasses at slaughter.

The primary objective of this 7-month study was to determine the prevalence of porcine pathogens of the tonsil of the soft palate of swine a … commonly isolated bacteria included: Streptococcus suis (53.7%), Arcanobacterium pyogenes (29.9%), Pasteurella multocida (27.3%), and Streptococcus porcinus (19.5%). Virus screening revealed evidence of PRRSV and PCV-2 in 22.0% and 11.9% of the samples, respectively. Salmonella Typhimurium and Yersinia enterocolitica were isolated in 0.5% and 1.8% of the samples, resp …

PCR evaluation of false-positive signals from two automated blood-culture systems.

Rapid detection of micro-organisms from blood is one of the most critical functions of a diagnostic microbiology laboratory. Automated blood … PCR amplicons indicated the presence of the following bacteria (number of isolates in parentheses): Pasteurella multocida (1), Staphylococcus epidermidis (2), Staphylococcus hominis (1), Micrococcus sp. (1), Streptococcus pneumoniae (1), Corynebacterium spp. (2), Brachibacterium sp. (1) and Arthrobacter/Rothia sp. (1). Antibiotic usage by the patients may be responsibl …

Hyaluronan biosynthesis by class I streptococcal hyaluronan synthases occurs at the reducing end.

Previous studies reached different conclusions about whether class I hyaluronan synthases (HASs) elongate hyaluronic acid (HA) by addition t … s turn over during HA biosynthesis. In contrast, HA-[32P]UDP products made by the purified class II Pasteurella multocida HAS were not released by adding unlabeled UDP-sugars, consistent with growth at the nonreducing end for this enzyme. The results demonstrate that the streptococcal class I HAS enzymes polymerize HA chains at the reducing end.

Epidemiologic and clinical aspects of animal bite injuries.

During 1975, 332 animal bite injuries accounted for 1.2% of all surgical problems treated at the UCLA Hospital Emergency Department. Data on … and 29% of cat bite-scratch victims returned with complications, mostly cellulitis or lymphangitis. Pasteurella multocida was the most common pathogen cultured, as evidenced by the 50% and 80% culture-positive rates for dog and cat bite-scratches respectively in this series.

Comparison of short nasal swab and deep nasopharyngeal swab sampling methods to describe BRD-associated viruses and bacteria detected using a metagenomics approach optimized for virus recovery in fall-placed beef calves shortly after feedlot arrival.

Short nasal swabs (SNS) have potential advantages of lower costs, collection time and training of personnel than deep nasopharyngeal swabs ( … 4 reads) more frequently in SNS than DNPS (mean reads: 169 versus 57; OR = 5.73, P = 0.001), as was Pasteurella multocida (≥ 1 read) (mean reads: 4.0 versus 1.2; OR = 2.02, P = 0.02). Mesomycoplasma dispar was less prevalent in SNS (mean reads: 5.2 versus 29; OR = 0.27, P = 0.001). Detection of Histophilus somni, Bibersteinia trehalosi, and Mycoplasmopsis bovis did not …