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Metabolomic Evidence of Biological Overlap with Heart Failure with Preserved Ejection Fraction in a Subset of Pulmonary Arterial Hypertension.

Rationale: A subset of patients with group 1 pulmonary hypertension (PH) have superimposed left heart abnormalities with unclear metabolic i … ll, n = 193, t test). Linoleate (precursor to arachidonic acid and prostaglandins) and arginine and homoarginine (precursors to nitric oxide) were all lower in group 1 PH + high HFpEF probability (FDR P < 0.03 for all, n = 193, t test). Metabolome changes in group 1 PH + high HFpEF probability overlapped with clinical HFpEF (n = 240) but were abnormal relative …

Origin an importance of increased alkaline phosphatase activity in peritoneal fluids of horses with colic.

The origin of increased alkaline phosphatase (ALP) activity in peritoneal fluid (PF) of horses with clinical signs of abdominal pain was investigated to determine the usefulness of measuring ALP in PF in the diagnosis of small intestinal injury. The ALP isoenzymes in PF from 10 clinically normal horses and from 50 horses with clinical signs of acute abdominal pain were analyzed for their sensitivities to inhibition by L-phenylalanine, L-homoarginine, and levamisole and to inactivation by heat (56 C, 15 minutes). The enzymes also were discriminated by their patterns of migration during polyacry…

Retrovirus-mediated gene transfer into rat salivary gland cells in vitro and in vivo.

A retroviral vector DAP that encodes the human placental alkaline phosphatase (PLAP) and the neomycin-resistant gene was used to transduce the salivary gland-derived cell line A5 in vitro and acinar cells in rat submandibular gland in vivo. Expression of the transduced PLAP gene was established by histochemical staining for heat-resistant AP and by determination of enzyme activity. From the in vitro experiments, we concluded that the salivary gland-derived cell line A5 can be infected by the retroviral vector DAP. In the transduced cells the viral long terminal repeat (LTR) promoter was effect

Effects of treatment with SGLT-2 inhibitors on arginine-related cardiovascular and renal biomarkers.

BACKGROUND: In patients with type 2 diabetes (T2D) sodium-glucose cotransporter 2 (SGLT-2) inhibitors improve glycaemic control as well as cardiovascular and renal outcomes. Their effects on L-arginine (Arg) related risk markers asymmetric and symmetric dimethylarginine (ADMA and SDMA) and the protective biomarker L-homoarginine (hArg) linking T2D to cardiovascular and renal disease have not yet been reported. METHODS: Plasma and 24-h urine samples taken before and after 6 weeks of treatment were available from two prospective, randomized, double-blind, placebo-controlled, cross-over trials w…

Profile of urinary amino acids and their post-translational modifications (PTM) including advanced glycation end-products (AGEs) of lysine, arginine and cysteine in lean and obese ZSF1 rats.

Heart failure with preserved ejection fraction (HFpEF) is associated with high mortality and has an increasing prevalence associated with th … fferences in urinary amino acids excretion between L-ZSF1 and O-ZSF1 rats. The creatinine-corrected homoarginine excretion was twofold lower in the O-ZSF1 rats. We also observed distinct associations between the concentrations of serum glucose and urinary amino acids including their PTM and AGE metabolites in the L-ZSF1 and O-ZSF1 rats. Our study shows that PT …

Mechanism of age-dependent susceptibility and novel treatment strategy in glutaric acidemia type I.

Glutaric acidemia type I (GA-I) is an inherited disorder of lysine and tryptophan metabolism presenting with striatal lesions anatomically and symptomatically similar to Huntington disease. Affected children commonly suffer acute brain injury in the context of a catabolic state associated with nonspecific illness. The mechanisms underlying injury and age-dependent susceptibility have been unknown, and lack of a diagnostic marker heralding brain injury has impeded intervention efforts. Using a mouse model of GA-I, we show that pathologic events began in the neuronal compartment while enhanced l

Mass Spectrometry-Based Evaluation of the Bland-Altman Approach: Review, Discussion, and Proposal.

Reliable quantification in biological systems of endogenous low- and high-molecular substances, drugs and their metabolites, is of particula … considered. The results for several different endogenous substances, including nitrate, anandamide, homoarginine, creatinine and malondialdehyde in human plasma, serum and urine are discussed. In addition to the Bland-Altman approach, linear regression analysis and the Oldham-Eksborg method-comparison approaches were used and compared. Special emphasis was giv …

Transport of urokinase across the intestinal tract of normal human subjects with stimulation of synthesis and/or release of urokinase-type proteins.

Oral administration of clinical-type high molecular weight urokinase (HMW-UK) in a single dose of 30,000-60,000 International Units (IU) in … a sequential two-step affinity chromatography method first with [N alpha-(epsilon-aminocaproyl)-DL-homoarginine hexylester]-Sepharose and second with a specific rabbit anti-HMW-UK-IgG-Sepharose. The yield of UK-type proteins from the 7-d group, 0.79 mg/dl, was approximately twofold greater than that obtained from either the placebo or 1-d groups. The specific …

Sanleng Wan inhibits endometriosis progression by regulating sphingolipid metabolism via the S1P/S1PR1-Akt axis based on serum metabolomics and network pharmacology.

ETHNOPHARMACOLOGICAL RELEVANCE: Endometriosis is a gynecological disorder characterized by chronic pelvic pain and infertility in women. The … the serum of the SLW-treated group, including L-threonine, (S)-homostachydrine, DL-arginine, and L-homoarginine, among others. These metabolites were involved in various metabolic pathways, including sphingolipid signaling, tyrosine metabolism, fatty acid degradation, arachidonic acid metabolism, pyruvate metabolism, linoleic acid metabolism, and glycerophosp …

Glyoxalase 1 sustains the metastatic phenotype of prostate cancer cells via EMT control.

Metastasis is the primary cause of death in prostate cancer (PCa) patients. Effective therapeutic intervention in metastatic PCa is undermined by our poor understanding of its molecular aetiology. Defining the mechanisms underlying PCa metastasis may lead to insights into how to decrease morbidity and mortality in this disease. Glyoxalase 1 (Glo1) is the detoxification enzyme of methylglyoxal (MG), a potent precursor of advanced glycation end products (AGEs). Hydroimidazolone (MG-H1) and argpyrimidine (AP) are AGEs originating from MG-mediated post-translational modification of proteins at arg

Longitudinal characterization of plasma amino acids, biogenic amines, and amino acid-related metabolites in heifers from birth to first calving in response to colostrum and transition milk feeding.

This study presents the first comprehensive longitudinal metabolomic profiling of plasma AA, biogenic amines, and AA-related metabolites in … al maturation. Nitrogen metabolism intermediates (citrulline and ornithine, decreasing postweaning; homoarginine, increasing at calving; phenylacetylglutamine, showing transient changes) reflected urea cycle and nitrogen disposal adjustments. Other metabolites, such as γ-aminobutyric acid, taurine, and lactate, displayed patterns indicative of neuroendocrine s …

Интеграция машинного обучения и бипло ГПЭ для идентификации пригодных для климата генотипов кустарников.

Граспея является богатой питательными веществами пищевой культурой, известной своей стойкостью в сложных агроэкосистемах. Вместе с тем информация о рекомендациях генотипов кузнепия относительно их пригодности как для общей, так и для конкретной адаптации является недостаточной. Главная цель исследования заключалась в определении стабильных генотипов кустарников путем сведения к минимуму влияния сложных взаимодействий между различными чертами окружающей среды. Кроме того, это исследование было направлено на выявление подходящих мест в различных агроклиматических зонах Индии для будущей оценки,

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… Notably, the presence of valuable nutraceuticals, such as L-homoarginine, further enhances its nutritional value ( Lambein, 2000 ; Das et al., 2021 ). …

Прогресс в развитии лекарственных средств на основе пептида: платформы для доставки, терапевтические средства и вакцины.

Успешное утверждение лекарств на основе пептида можно отнести на счет совместных усилий по целому ряду дисциплин. Интеграция новых методов разработки и синтеза лекарственных средств, отображение библиотечных технологий, систем доставки, достижений в области биоинженерии и искусственного интеллекта в значительной степени ускорила разработку новейших лекарств на основе пептида, эффективно устраняя препятствия, связанные с их характером, такие, как быстрое обезвреживание и деградация, необходимость субколичественной инъекции, ведущей к усилению дискомфорта пациентов, и в конечном итоге продвигая

Совпадение в полном тексте источника
… This substitution seeks to bring about advantages such as the augmentation of binding affinity and target selectivity. 201 Notable applications of side chain modifications include the incorporation of analogues like β-phenylalanine, 202 benzyloxytyrosine, 184 and homoarginine. 203 These modifications …

Артериальный тромбоэмболизм

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Совпадение в полном тексте источника
… ADMA, SDMA and L‐homoarginine, have emerged as biomarkers linked to cardiovascular outcomes. Aims : To investigate the association of SDMA with platelet reactivity and bleeding risk in patients with acute coronary syndrome (ACS) treated with potent P2Y12 inhibitors prasugrel and ticagrelor. …