Prostaglandins & other lipid mediators · 2025
Protection of lutein against the toxic effect of cisplatin on liver in male rat.
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Aktas Ibrahim, Gur Fatih Mehmet, Bilgiç Sedat
Аннотация
OBJECTIVE: A major challenge in cancer treatment is the detrimental effects of anticancer drugs on healthy organs and tissues. This study aims to investigate the protective effects of Lutein (LU) against Cisplatin (CT)-induced toxicity in rat liver, utilizing biochemical and histopathological assessments.
METHODS: In this study, CT was administered intraperitoneally (i.p.) at a dose of 10 mg/kg, while LU was administered orally at a dose of 100 mg/kg. The experiment was conducted over a 7-day period with 28 male Sprague-Dawley rats (weighing 210-265 g, aged 11 weeks), divided into four groups (n = 7): Control, LU, CT, and CT + LU.
RESULTS: CT-induced liver injury was identified as a dose-limiting side effect of CT. Compared to the CT group, the CT + LU group exhibited a significant decrease in malondialdehyde (MDA) levels and an increase in superoxide dismutase (SOD), catalase (CAT), and glutathione (GSH) levels. In the CT group, a significant increase in the levels of gamma-glutamyl transferase (GGT), alanine aminotransferase (ALT), aspartate aminotransferase (AST), and lactate dehydrogenase (LDH) was observed, compared to the control group (p < 0.05). When comparing the CT + LU group with the CT group, a significant reduction in the levels of GGT, ALT, AST, and LDH was observed (p < 0.05). Histopathological analysis revealed liver damage in the CT group, characterized by leukocyte infiltration, sinusoidal dilatation, Councilman body formation, and hepatocellular degeneration and steatosis. In contrast, the CT + LU group exhibited only mild sinusoidal dilatation, with no other significant lesions. Immunohistochemical analysis showed positive staining for tumour necrosis factor-alpha (TNF-α) and caspase-3 in the liver tissue of CT group rats, which was significantly reduced in the CT + LU group. The staining pattern in the CT + LU group was similar to that of the control and LU groups.
CONCLUSION: The results of this study suggest that LU mitigates oxidative stress, enhances antioxidant defences, and supports liver function. Furthermore, LU demonstrates a protective effect against CT-induced liver damage, indicating its potential as a pharmacological agent for preventing CT-induced hepatic injury.
METHODS: In this study, CT was administered intraperitoneally (i.p.) at a dose of 10 mg/kg, while LU was administered orally at a dose of 100 mg/kg. The experiment was conducted over a 7-day period with 28 male Sprague-Dawley rats (weighing 210-265 g, aged 11 weeks), divided into four groups (n = 7): Control, LU, CT, and CT + LU.
RESULTS: CT-induced liver injury was identified as a dose-limiting side effect of CT. Compared to the CT group, the CT + LU group exhibited a significant decrease in malondialdehyde (MDA) levels and an increase in superoxide dismutase (SOD), catalase (CAT), and glutathione (GSH) levels. In the CT group, a significant increase in the levels of gamma-glutamyl transferase (GGT), alanine aminotransferase (ALT), aspartate aminotransferase (AST), and lactate dehydrogenase (LDH) was observed, compared to the control group (p < 0.05). When comparing the CT + LU group with the CT group, a significant reduction in the levels of GGT, ALT, AST, and LDH was observed (p < 0.05). Histopathological analysis revealed liver damage in the CT group, characterized by leukocyte infiltration, sinusoidal dilatation, Councilman body formation, and hepatocellular degeneration and steatosis. In contrast, the CT + LU group exhibited only mild sinusoidal dilatation, with no other significant lesions. Immunohistochemical analysis showed positive staining for tumour necrosis factor-alpha (TNF-α) and caspase-3 in the liver tissue of CT group rats, which was significantly reduced in the CT + LU group. The staining pattern in the CT + LU group was similar to that of the control and LU groups.
CONCLUSION: The results of this study suggest that LU mitigates oxidative stress, enhances antioxidant defences, and supports liver function. Furthermore, LU demonstrates a protective effect against CT-induced liver damage, indicating its potential as a pharmacological agent for preventing CT-induced hepatic injury.