Cell stem cell · 2021
Patient-derived organoids model cervical tissue dynamics and viral oncogenesis in cervical cancer.
СтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод в очереди
Обработка статьи · этапы, ошибки и управление
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Lõhmussaar Kadi, Oka Rurika, Espejo Valle-Inclan Jose, Smits Milou H H, Wardak Hila, Korving Jeroen, Begthel Harry, Proost Natalie, van de Ven Marieke, Kranenburg Onno W, Jonges Trudy G N, Zweemer Ronald P, Veersema Sebastiaan, van Boxtel Ruben, Clevers Hans
Аннотация
Cervical cancer is a common gynecological malignancy often caused by high-risk human papillomavirus. There is a paucity of human-derived culture systems to study the cervical epithelium and the cancers derived thereof. Here we describe a long-term culturing protocol for ecto- and endocervical epithelia that generates 3D organoids that stably recapitulate the two tissues of origin. As evidenced for HSV-1, organoid-based cervical models may serve to study sexually transmitted infections. Starting from Pap brush material, a small biobank of tumoroids derived from affected individuals was established that retained the causative human papillomavirus (HPV) genomes. One of these uniquely carried the poorly characterized HPV30 subtype, implying a potential role in carcinogenesis. The tumoroids displayed differential responses to common chemotherapeutic agents and grew as xenografts in mice. This study describes an experimental platform for cervical (cancer) research and for future personalized medicine approaches.