Advances in experimental medicine and biology · 2010
Effect of 3(')-daidzein sulfonic sodium on the anti-oxidation of retinal ischemia/reperfusion injury in rats.
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Zhihua Huang, Liangdong Li, Xiao Li, Fang Cheng, Jing Zeng
Аннотация
OBJECTIVE: To research the 3(')-daidzein sulfonic sodium's (DSS) effect on anti-oxidation of retinal ischemia/reperfusion (RI/R) injury in rats.
METHODS: RI/R in rats was made by reperfusion for 1 h after occlusion of common carotid artery (CCA) for 1 h and the rats were divided into four groups randomly, including sham control group, model group of RI/R injury, low-dosage DSS group and high-dosage DSS group. 1 ml/kg NS was injected through sublingual vein after CCA was dissociated in sham group. Other groups were treated with normal saline, low-dosage DSS (1 mg/kg) and high-dosage DSS (2 mg/kg) through sublingual vein respectively before occlusion of CCA. After occlusion of CCA for 1 h and reperfusion for 1 h, blood was put out and separated into the serum. Then detect the contents of MDA and NO, the activity of SOD and GSH-PX.
RESULT: (1) The content of MDA was increased in model group (P<0.05), and low-dosage DSS can reverse it (P<0.05). (2) The content of serum NO in model group was lower than in sham group (P<0.001). The content of serum NO in model group is lower than in low-dosage DSS group (P<0.05) and high-dosage DSS group (P<0.01) respectively. (3) The activity of GSH-PX in high-dosage group was higher than in model group (P<0.05). (4) The activity of SOD in low-dosage group was higher than in model group (P<0.05).
CONCLUSION: DSS can decrease the cellular damage and protect the optical function in RI/R injury through elevating the content of NO, the activity of SOD and GSH-PX, and increasing anti-oxidation.
METHODS: RI/R in rats was made by reperfusion for 1 h after occlusion of common carotid artery (CCA) for 1 h and the rats were divided into four groups randomly, including sham control group, model group of RI/R injury, low-dosage DSS group and high-dosage DSS group. 1 ml/kg NS was injected through sublingual vein after CCA was dissociated in sham group. Other groups were treated with normal saline, low-dosage DSS (1 mg/kg) and high-dosage DSS (2 mg/kg) through sublingual vein respectively before occlusion of CCA. After occlusion of CCA for 1 h and reperfusion for 1 h, blood was put out and separated into the serum. Then detect the contents of MDA and NO, the activity of SOD and GSH-PX.
RESULT: (1) The content of MDA was increased in model group (P<0.05), and low-dosage DSS can reverse it (P<0.05). (2) The content of serum NO in model group was lower than in sham group (P<0.001). The content of serum NO in model group is lower than in low-dosage DSS group (P<0.05) and high-dosage DSS group (P<0.01) respectively. (3) The activity of GSH-PX in high-dosage group was higher than in model group (P<0.05). (4) The activity of SOD in low-dosage group was higher than in model group (P<0.05).
CONCLUSION: DSS can decrease the cellular damage and protect the optical function in RI/R injury through elevating the content of NO, the activity of SOD and GSH-PX, and increasing anti-oxidation.