Journal of ethnopharmacology · 2026
Polyphenolic profile and gastroprotective effect of Jatropha multifida Linn leaves against ethanol-induced gastric ulcer in rats: Involvement of the NLRP3/caspase-1/GSDMD pyroptotic pathway.
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Abdallah Arwa K, El-Sayed Elsayed K, Mohamed Esraa M, Khattab Mohamed A, Ahmed Asmaa A, Tawfik Nashwa F, Moharram Fatma A
Аннотация
ETHNOPHARMACOLOGICAL RELEVANCE: Gastric ulcer is a widespread gastrointestinal disorder that continues to warrant ongoing research into improved therapeutic strategies. Jatropha multifida (J. multifida) Linn has demonstrated efficacy in various diseases and is traditionally used for gastrointestinal and ulcer-related disorders.
AIM OF THE STUDY: To tentatively identify phenolic metabolites and measure the total phenolic (TPC) and flavonoids (TFC) in the defatted fraction (DF) of J. multifida and assess their in vivo antiulcer activity.
MATERIALS AND METHODS: The tentative identification of the phenolic compounds was performed using HPLC/MS. Additionally, the TPC and TFC were quantified using the Folin-Ciocalteu colorimetric method and the AlCl3 method. Rats were randomized into six groups (n = 6): control, ethanol-ulcer, sucralfate (100 mg/kg), and three J. multifida DF-treated groups (250, 500, 1000 mg/kg). Stomachs were collected for ulcer assessment, histology, and biochemical analyses.
RESULTS: Our findings showed that TPC and TFC in J. multifida are 26.066 ± 0.09 mg GAE/g and 10.161 ± 0.17 mg CE/g, respectively. A total of sixty-four compounds were tentatively identified by HPLC/MS, comprising phenolic acids, flavonoids, proanthocyanidins, lignans, coumarins, and stilbenes. J. multifida DF lowered ulcer score and gastric volume, increased gastric pH and mucin content, reduced oxidative stress, inflammatory and pyroptotic markers, and restored GSH and PGE2 levels, with histology confirming marked protection against ethanol-induced gastric injury.
CONCLUSION: The gastroprotective effect of J. multifida DF is mediated by enhanced mucus production and suppression of oxidative stress and inflammation through inhibition of the NLRP3/Caspase-1/GSDMD pyroptotic pathway, supporting its potential as a therapeutic candidate for peptic ulcer disease.
AIM OF THE STUDY: To tentatively identify phenolic metabolites and measure the total phenolic (TPC) and flavonoids (TFC) in the defatted fraction (DF) of J. multifida and assess their in vivo antiulcer activity.
MATERIALS AND METHODS: The tentative identification of the phenolic compounds was performed using HPLC/MS. Additionally, the TPC and TFC were quantified using the Folin-Ciocalteu colorimetric method and the AlCl3 method. Rats were randomized into six groups (n = 6): control, ethanol-ulcer, sucralfate (100 mg/kg), and three J. multifida DF-treated groups (250, 500, 1000 mg/kg). Stomachs were collected for ulcer assessment, histology, and biochemical analyses.
RESULTS: Our findings showed that TPC and TFC in J. multifida are 26.066 ± 0.09 mg GAE/g and 10.161 ± 0.17 mg CE/g, respectively. A total of sixty-four compounds were tentatively identified by HPLC/MS, comprising phenolic acids, flavonoids, proanthocyanidins, lignans, coumarins, and stilbenes. J. multifida DF lowered ulcer score and gastric volume, increased gastric pH and mucin content, reduced oxidative stress, inflammatory and pyroptotic markers, and restored GSH and PGE2 levels, with histology confirming marked protection against ethanol-induced gastric injury.
CONCLUSION: The gastroprotective effect of J. multifida DF is mediated by enhanced mucus production and suppression of oxidative stress and inflammation through inhibition of the NLRP3/Caspase-1/GSDMD pyroptotic pathway, supporting its potential as a therapeutic candidate for peptic ulcer disease.