Effect of Glucocorticoid Nanoliposomes on Vascular Endothelial Growth Factor Expression in Brain Tissue of Rats with Tuberculous Meningitis.

To investigate the effect of human brain-targeted nanoliposomes encapsulating methylprednisolone sodium succinate on the level of vascular endothelial growth factor (VEGF) in brain tissue of rats with tuberculous meningitis (TBM), the nanoliposome DSPE-125I-AIBZM-MPS was prepared. 180 rats were divided into normal control, TBM infection, and TBM treatment groups. The brain water content, Evans blue (EB) content, VEGF, and the gene and protein expression of receptors (Flt-1, Flk-1) of rats after modeling were measured. The brain water content and EB content in the TBM treatment group were significantly lower than those in the TBM infection group at 4 and 7 days after modeling (P < 0.05). The expression of VEGF and its receptor Flt-1 mRNA in the brain tissue of rats in the TBM infection group was significantly higher than that in the normal control group at 1, 4, and 7 days after modeling (P < 0.05). The expression of VEGF and its receptor Flt-1 mRNA in the brain tissue of rats in the TBM treatment group was significantly higher than that in the TBM infection group at 1, 4, and 7 days after modeling (P < 0.05). In summary, the prepared DSPE-125I-AIBZM-MPS nanoliposomes can effectively reduce brain water content and EB content and reduce the release of inflammatory factors of brain tissue in rats, playing a role in the treatment of TBM in rats by regulating the expression of VEGF and its receptor Flt-1 mRNA.