[Equivalence and mechanism of standard decoction and formula granules of calcined oyster in treatment of rats with ethanol-induced gastric ulcers based on serum metabolomics].

An ethanol-induced gastric ulcer model was replicated to investigate the equivalence and metabolic regulatory mechanisms of formula granules and standard decoction of calcined oyster by using metabolomics. The ethanol-induced gastric ulcer model was established, and the dose-effect curve was fitted with the ulcer inhibition rate as the effect to compare the equal-effect dose(DEE) and equal-dose effect(EED) of standard decoction and formula granules of calcined oyster. The formula granule group and standard decoction group with the equivalent effect were selected for further pharmacodynamic index detection, and metabolomics analysis was performed on the serum to explore the metabolic regulation mechanism of formula granules and standard decoction of calcined oyster. According to the dose-effect curve equation, the DEE and EED of formula granules and standard decoction were calculated. Formula granules and standard decoction of calcined oyster could improve the body's oxidative stress and inflammatory factor level, increase the level of protective factors, and treat ethanol-induced gastric ulcers. Serum metabolomics analysis showed that formula granules and standard decoction of calcined oyster mainly treated ethanol-induced gastric ulcers through eight potential metabolic pathways, and the metabolic regulation of standard decoction and formula granules on gastric ulcer was different. Within the corresponding effective range, 1 g·kg~(-1) standard decoction was pharmacologically equivalent to 1.07 g·kg~(-1) formula granules in treating ethanol-induced gastric ulcers. Formula granules and standard decoction of calcined oyster mainly treat ethanol-induced gastric ulcers by regulating metabolic disorders in pathways such as primary bile acid biosynthesis.