Cytomorphological characteristics of the Nasopharyngeal zone mucosa in children with Covid-19.

OBJECTIVE

Aim: To investigate cytomorphological presentations of the mucous membrane damage of the nasopharyngeal zone in Coronavirus disease in children.

PATIENTS AND

METHODS

Materials and

Methods

A epithelium scraping from the surface of the Pharyngeal tonsils was performed with the following double fixation: the samples were fixed in a 2% solution of glutaraldehyde (25% Glutaraldehyde E. M. Grade Polyscieces Inc.) in 0.1M cacodylate buffer (Cacodylic acid Sodium salt. Fluka) at pH 7.2 for 2-2.5 hours in the cold. Washing (2 times) in cacodylate buffer was performed. The next stage was additional fixation of the samples in a 1.5% solution of OsO4 (Osmium Tetroxide. SPI - CHEM USA) in 0.1 M sodium cacodylate solution at pH 7.2 for 2 hours in the cold with subsequent washing (2 times) in buffer. Sections are made on an UMTP-6M ultramicrotome using a diamond knife (DIATOM). Sections were viewed using a TEM-100 transmission electron microscope (Ukraine).

RESULTS

Results

Based on the electronic presentation, we can observe all the processes of inflammation and their consequences. We will consider the pathogenetic processes which were monitored in our studies. Inflammation consists of three main types of tissue disorders, which include: alteration, exudation, proliferation. Presentation of a section of the Palatine Tonsil epithelial cell is marked by increased focal hemorrhage and degenerative changes in the membrane of papillary structures, reactive-degenerative changes in the stroma and increased hemorrhages in the vessels, partial sclerosis and microcalcification of the Tonsil epithelial cell tissue, pronounced sclerotic changes in the epithelial cell and partial destruction of the cell membrane were also visualized, as a result of which the semiconductivity in the cell is disturbed.

CONCLUSION

Conclusions

Cytological changes in the studied material of Nasopharyngeal zone in the children with Coronavirus disease can be considered as nonspecific common violation. These changes were characterized by the presence of diffuse inflammatory polymorpho-cellular infiltration, impaired hemodynamics and diffuse desquamative, dystrophic, sclerotic changes in epithelial cells, which are consistent with numerous data of scientists.