Medical mycology · 2019
Potassium iodide and miltefosine inhibit biofilms of Sporothrix schenckii species complex in yeast and filamentous forms.
СтатьяНазвание и аннотация: Перевод в очереди
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Brilhante Raimunda Sâmia Nogueira, Silva Maria Lucilene Queiroz da, Pereira Vandbergue Santos, de Oliveira Jonathas Sales, Maciel Juliana Maria, Silva Isaac Neto Goes da, Garcia Lana Glerieide Silva, Guedes Glaucia Morgana de Melo, Cordeiro Rossana de Aguiar, Pereira-Neto Waldemiro de Aquino, de Camargo Zoilo Pires, Rodrigues Anderson Messias, Sidrim José Júlio Costa, Castelo-Branco Débora de Souza Collares Maia, Rocha Marcos Fábio Gadelha
Аннотация
This study aimed to evaluate the yeast biofilm growth kinetics and ultrastructure of Sporothrix schenckii complex and assess their mature biofilm susceptibility in filamentous and yeast forms to potassium iodide (KI) and miltefosine (MIL). Yeast biofilms were evaluated by crystal violet staining, XTT reduction assay and microscopic techniques. Susceptibility of planktonic and sessile cells was analyzed by broth microdilution. S. schenckii complex in yeast form produced biofilms, with an optimum maturation at 96 h, showing multilayered blastoconidia embedded in extracellular matrix. KI and MIL minimum inhibitory concentration (MIC) ranges against planktonic cells were 62,500-250,000 μg/ml and 0.125-4 μg/ml, respectively. KI and MIL reduced biofilm metabolic activity by 75.4% and 67.7% for filamentous form and 55.1% and 51.6% for yeast form, respectively. This study demonstrated that S. schenckii complex forms biofilms in vitro, and potassium iodide and miltefosine inhibit Sporothrix spp. biofilms in both filamentous and yeast forms.