Auris, nasus, larynx · 2025
Effects of intratympanic administration of glucocorticoids on the guinea pig inner ear.
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Kakigi Akinobu, Kinoshita Makoto, Katsutani Yuki, Sahara Toshihito, Yamashita Shinji, Kondo Kenji
Аннотация
OBJECTIVE: This study investigates the effects of intratympanic glucocorticoid administration on the inner ear.
METHODS: Fifteen Hartley guinea pigs were used and divided into five groups: dexamethasone-treated (n = 5), contralateral dexamethasone-treated (n = 5), hydrocortisone-treated (n = 5), contralateral hydrocortisone-treated (n = 5), and control (n = 5). In the control group, 1 ml of saline was injected into the right tympanic cavity via a ventral approach. In the dexamethasone-treated group, 1 ml of dexamethasone at a concentration of 3.3 mg/ml was injected into the right tympanic cavity using the same method, while the left ear of the dexamethasone-treated group remained intact. In the hydrocortisone-treated group, 1 ml of hydrocortisone at a concentration of 50 mg/ml was injected into the right tympanic cavity using the same technique, with the left ear of the hydrocortisone-treated group also remaining intact. To assess the effects of glucocorticoids on the inner ear, we measured a cross-sectional area analysis of the scala media at three hours post-corticosteroids treatment using optical coherence tomography (OCT). The degree of a cross-sectional area change was quantitatively measured using a video digitizer.
RESULTS: Ears treated with either dexamethasone or hydrocortisone showed extension of Reissner's membrane and endolymphatic hydrops. Statistical analysis indicated that a cross-sectional area of the scala media increased significantly compared to the contralateral hydrocortisone-treated and control groups.
CONCLUSIONS: This is the first report to investigate the effects of intratympanic administration of glucocorticoids on the guinea pig cochlea. Dilated endolymphatic space was observed in the steroid-treated ears. Although the underlying mechanism of these histological changes remains unclear, great care must be taken when administering steroids to patients with endolymphatic hydrops-related diseases, such as Meniere's disease.
METHODS: Fifteen Hartley guinea pigs were used and divided into five groups: dexamethasone-treated (n = 5), contralateral dexamethasone-treated (n = 5), hydrocortisone-treated (n = 5), contralateral hydrocortisone-treated (n = 5), and control (n = 5). In the control group, 1 ml of saline was injected into the right tympanic cavity via a ventral approach. In the dexamethasone-treated group, 1 ml of dexamethasone at a concentration of 3.3 mg/ml was injected into the right tympanic cavity using the same method, while the left ear of the dexamethasone-treated group remained intact. In the hydrocortisone-treated group, 1 ml of hydrocortisone at a concentration of 50 mg/ml was injected into the right tympanic cavity using the same technique, with the left ear of the hydrocortisone-treated group also remaining intact. To assess the effects of glucocorticoids on the inner ear, we measured a cross-sectional area analysis of the scala media at three hours post-corticosteroids treatment using optical coherence tomography (OCT). The degree of a cross-sectional area change was quantitatively measured using a video digitizer.
RESULTS: Ears treated with either dexamethasone or hydrocortisone showed extension of Reissner's membrane and endolymphatic hydrops. Statistical analysis indicated that a cross-sectional area of the scala media increased significantly compared to the contralateral hydrocortisone-treated and control groups.
CONCLUSIONS: This is the first report to investigate the effects of intratympanic administration of glucocorticoids on the guinea pig cochlea. Dilated endolymphatic space was observed in the steroid-treated ears. Although the underlying mechanism of these histological changes remains unclear, great care must be taken when administering steroids to patients with endolymphatic hydrops-related diseases, such as Meniere's disease.