Life stage dependent effects of neonicotinoid exposure on honey bee hypopharyngeal gland development.

Functional Apis mellifera honey bee colonies rely on collaborative brood care typically performed by nurse bees with well-developed hypopharyngeal glands (HPGs). Neonicotinoids, widely used insecticides, have been shown to negatively affect HPG development when worker bees were exposed to field-realistic concentrations either as brood or adults. To date, it is unknown whether timing of neonicotinoid exposure influences the severity of these observed negative effects on HPGs. To address this, we conducted a fully-crossed field experiment assessing potential effects of a neonicotinoid blend (clothianidin and thiamethoxam combined) on worker HPGs when exposed during different life stages. We found that neonicotinoid exposure during the brood stage, but not the adult stage, significantly influenced subsequent HPG development. Since HPG morphogenesis begins during the brood stage, neonicotinoid-induced stress possibly impaired this process, resulting in smaller glands once these individuals became adult nurses. Because HPG productivity is correlated to their size, smaller glands as a result of neonicotinoid exposure could negatively affect colony functionality.